##gff-version 3
##sequence-region BBa_J31000 1 573
BBa_J31000	.	sequence_alteration	70	75	.	+	0	ID=annotation1880459;Name=Former SpeI site
BBa_J31000	.	CDS	1	573	.	+	0	ID=annotation1884987;Name=Hin sequence
BBa_J31000	.	sequence_alteration	72	72	.	+	0	ID=annotation1880460;Name=Mutation to delete the SpeI site
BBa_J31000	.	primer_binding_site	1	3	.	+	0	ID=annotation1910568;Name=Fwd primer J31001
BBa_J31000	.	primer_binding_site	551	570	.	+	0	ID=annotation1910569;Name=Rev primer J31001
>BBa_J31000
atggctactattgggtatattcgggtgtcaacaattgaccaaaatatcgatttacagcgtaatgcgcttaccagtgcaa
attgtgaccgcatttttgaagaccgtatcagtggcaagattgcaaaccgccccggcctgaaacgggcgttaaagtatgt
aaataaaggcgatactcttgtcgtctggaaattagacagactgggccgtagcgtgaaaaatctggtggcgttaatatca
gaattacatgaacgtggagctcacttccattctttaaccgatagtattgataccagtagcgcgatggggcgattctttt
ttcatgtaatgtcagcactggccgagatggagcgagaattaatcgtcgagcgaacccttgccggactggctgccgccag
agcgcaaggacgactgggagggcgccctcgggcgatcaacaaacatgaacaggaacagattagtcggctattagagaaa
ggccatcctcggcagcaattagctattatttttggtattggcgtatccaccttatacagatactttccggcaagcagta
taaaaaaacgaatgaattaa