BBa_J44001 1 BBa_J44001 Reverse RBS (RBS_rev) -- corresponds to BBa_B0030 2006-08-01T11:00:00Z 2015-08-31T04:08:48Z Cloned from synthetic oligos Released HQ 2013 Reverse version of RBS BBa_B0030 false false _61_71_ 0 606 61 In stock true Repeats in oligos caused unusual products during cloning true Todd Eckdahl annotation1893199 1 Reverse RBS range1893199 1 1 15 BBa_J31010 1 BBa_J31010 KanB : RBSrev 2006-10-27T11:00:00Z 2015-08-31T04:08:45Z Parts BBa_J31002 and BBa_J44001 Kanamyin resistance backwards, followed by RBS backwards true false _61_ 0 1144 61 Discontinued false Kan was PCR-amplified with primers designed to introduce a SpeI site to the left and an XbaI site to the right (reversing the normal order of these two BioBrick cut sites. The product was cut with SpeI and XbaI and ligated into S/X cut pSB1A2. The source for this vector was pSB1A2 carrying RFP. After cloning, we used a S/X digest to check for insertion in the desired oreitnation (reverse). Clones where Kan did not excise inserted in the wrong way (failure to cut is due to S/X mixed sites) and clones where Kan excised successfully inserted in the desired orientation (restoration of S and X sites). false Karmella Haynes component1908107 1 BBa_J31002 component1908109 1 BBa_J44001 annotation1908107 1 BBa_J31002 range1908107 1 1 816 annotation1908109 1 BBa_J44001 range1908109 1 825 839 BBa_J31002 1 KanR Backw kanamycin resistance backwards (KanB) [cf. BBa_J23012 & BBa_J31003] 2006-07-11T11:00:00Z 2015-08-31T04:08:45Z pSB1AK3 Codes the reverse of the Kanamycin Resistance gene. When the region is flipped, Kanamycin resistance will be expressed as long as a promoter and RBS are in front of the region. false false _61_ 0 918 61 It's complicated false None true Erin Zwack, Sabriya Rosemond annotation1884995 1 Reverse of KanR gene range1884995 1 1 816 BBa_J44001_sequence 1 tttctcctctttaat BBa_J31010_sequence 1 aatctttttgagtagctcgtagtttactttgacgttaaataagtatagtcctaatagttatggtataaaaactttttcggcaaagacattacttcctcttttgagtggctccgtcaaggtatcctaccgttctaggaccatagccagacgctaaggctgagcaggttgtagttatgttggataattaaaggggagcagtttttattccaatagttcactctttagtggtactcactgctgacttaggccactcttaccgttttcgaatacgtaaagaaaggtctgaacaagttgtccggtcggtaatgcgagcagtagttttagtgagcgtagttggtttggcaataagtaagcactaacgcggactcgctctgctttatgcgctagcgacaattttcctgttaatgtttgtccttagcttacgttggccgcgtccttgtgacggtcgcgtagttgttataaaagtggacttagtcctataagaagattatggaccttacgacaaaagggcccctagcgtcaccactcattggtacgtagtagtcctcatgcctattttacgaactaccagccttctccgtatttaaggcagtcggtcaaatcagactggtagagtagacattgtagtaaccgttgcgatggaaacggtacaaagtctttgttgagaccgcgtagcccgaagggtatgttagctatctaacagcgtggactaacgggctgtaatagcgctcgggtaaatatgggtatatttagtcgtaggtacaaccttaaattagcgccggacctcgttctgcaaagggcaacttataccgagtatactagagtttctcctctttaat BBa_J31002_sequence 1 ttagaaaaactcatcgagcatcaaatgaaactgcaatttattcatatcaggattatcaataccatatttttgaaaaagccgtttctgtaatgaaggagaaaactcaccgaggcagttccataggatggcaagatcctggtatcggtctgcgattccgactcgtccaacatcaatacaacctattaatttcccctcgtcaaaaataaggttatcaagtgagaaatcaccatgagtgacgactgaatccggtgagaatggcaaaagcttatgcatttctttccagacttgttcaacaggccagccattacgctcgtcatcaaaatcactcgcatcaaccaaaccgttattcattcgtgattgcgcctgagcgagacgaaatacgcgatcgctgttaaaaggacaattacaaacaggaatcgaatgcaaccggcgcaggaacactgccagcgcatcaacaatattttcacctgaatcaggatattcttctaatacctggaatgctgttttcccggggatcgcagtggtgagtaaccatgcatcatcaggagtacggataaaatgcttgatggtcggaagaggcataaattccgtcagccagtttagtctgaccatctcatctgtaacatcattggcaacgctacctttgccatgtttcagaaacaactctggcgcatcgggcttcccatacaatcgatagattgtcgcacctgattgcccgacattatcgcgagcccatttatacccatataaatcagcatccatgttggaatttaatcgcggcctggagcaagacgtttcccgttgaatatggctcat igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z