BBa_J31011 1 BBa_J31011 RBS-RFP reverse 2006-10-28T11:00:00Z 2015-08-31T04:08:45Z BBa_I13507 BBa_J31010 was created by PCR amplification of the RBS-RFP portion of BBa_I13507. true false _61_ 0 1144 61 Discontinued false The primers were designed to add PstI-NotI-SpeI to the left and XbaI-NotI-EcoRI to the right (reversal of standard BioBrick cloning sites) so that this part could be cloned in the reverse orientation. Placement of a promoter (reverse) to the right of this part should convey RFP expression. false Karmella Haynes annotation1908409 1 mRFP 1 range1908409 1 4 678 annotation1908411 1 misc range1908411 1 689 692 annotation1908410 1 B0034 range1908410 1 685 696 BBa_J31011_sequence 1 ttaagcaccggtggagtgacgaccttcagcacgttcgtactgttcaacgatggtgtagtcttcgttgtgggaggtgatgtccagtttgatgtcggttttgtaagcacccggcagctgaaccggttttttagccatgtaggtggttttaacttcagcgtcgtagtgaccaccgtctttcagtttcagacgcattttgatttcacctttcagagcaccgtcttccgggtacatacgttcggtggaagcttcccaacccatggtttttttctgcataaccggaccgtcggacgggaagttggtaccacgcagtttaactttgtagatgaactcaccgtcttgcagggaggagtcctgggtaacggtaacaacaccaccgtcttcgaagttcataacacgttcccatttgaaaccttccgggaaggacagtttcaggtagtccgggatgtcagccgggtgtttaacgtaagctttggaaccgtactggaactgcggggacaggatgtcccaagcgaacggcagcggaccacctttggtaactttcagtttagcggtctgggtaccttcgtacggacgaccttcaccttcaccttcgatttcgaactcgtgaccgttaacggaaccttccatacgaactttgaaacgcatgaactctttgataacgtcttcggaggaagccatctagtatttctcctcttt igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z