##gff-version 3 ##sequence-region BBa_J31012 1 1589 BBa_J31012 . terminator 89 129 . + 0 ID=BBa_B0012;Name=BBa_B0012 BBa_J31012 . stem_loop 96 115 . + 0 ID=annotation1686;Name=T7 TE;Parent=BBa_B0012 BBa_J31012 . polyA_site 116 129 . + 0 ID=annotation1690;Name=polya;Parent=BBa_B0012 BBa_J31012 . stop_codon 122 122 . + 0 ID=annotation1687;Name=stop;Parent=BBa_B0012 BBa_J31012 . engineered_region 89 129 . + 0 ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012 BBa_J31012 . terminator 1 80 . + 0 ID=BBa_B0010;Name=BBa_B0010 BBa_J31012 . stem_loop 12 55 . + 0 ID=annotation4184;Name=stem_loop;Parent=BBa_B0010 BBa_J31012 . engineered_region 1 80 . + 0 ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010 BBa_J31012 . sequence_feature 310 335 . + 0 ID=BBa_J44000;Name=BBa_J44000 BBa_J31012 . promoter 172 301 . + 0 ID=BBa_I13453;Name=BBa_I13453 BBa_J31012 . sequence_feature 1564 1589 . + 0 ID=BBa_J44000;Name=BBa_J44000 BBa_J31012 . CDS 365 1555 . + 0 ID=BBa_J31007;Name=BBa_J31007 BBa_J31012 . primer_binding_site 365 388 . + 0 ID=annotation1910571;Name=Fwd primer J31007;Parent=BBa_J31007 BBa_J31012 . primer_binding_site 1536 1555 . + 0 ID=annotation1910572;Name=Rev primer J31007;Parent=BBa_J31007 BBa_J31012 . CDS 365 1555 . + 0 ID=annotation1885000;Name=tetA(C) forward;Parent=BBa_J31007 BBa_J31012 . stop_codon 1553 1555 . + 0 ID=annotation1910573;Name=stop;Parent=BBa_J31007 BBa_J31012 . ribosome_entry_site 344 358 . + 0 ID=BBa_B0030;Name=BBa_B0030 BBa_J31012 . engineered_region 344 358 . + 0 ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030 BBa_J31012 . ribosome_entry_site 351 355 . + 0 ID=annotation1702;Name=RBS;Parent=BBa_B0030 BBa_J31012 . ribosome_entry_site 344 358 . + 0 ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030 BBa_J31012 . sequence_feature 138 163 . + 0 ID=BBa_J44000;Name=BBa_J44000 >BBa_J31012 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctct ctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagttatcaaaaaccatggttttt gataatactagagacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcgg atcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagttatcaa aaaccatggtttttgataatactagagattaaagaggagaaatactagatgaaatctaacaatgcgctcatcgtcatcc tcggcaccgtcaccctggatgctgtaggcataggcttggttatgccggtactgccgggcctcttgcgggatatcgtcca ttccgacagcatcgccagtcactatggcgtgctgctagcgctatatgcgttgatgcaatttctatgcgcacccgttctc ggagcactgtccgaccgctttggccgccgcccagtcctgctcgcttcgctacttggagccactatcgactacgcgatca tggcgaccacacccgtcctgtggatcctctacgccggacgcatcgtggccggcatcaccggcgccacaggtgcggttgc tggcgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtg ggtatggtggcaggccccgtggccgggggactgttgggcgccatctccttgcatgcaccattccttgcggcggcggtgc tcaacggcctcaacctactactgggctgcttcctaatgcaggagtcgcataagggagagcgtcgaccgatgcccttgag agccttcaacccagtcagctccttccggtgggcgcggggcatgactatcgtcgccgcacttatgactgtcttctttatc atgcaactcgtaggacaggtgccggcagcgctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatga tcggcctgtcgcttgcggtattcggaatcttgcacgccctcgctcaagccttcgtcactggccccgccaccaaacgttt cggcgagaagcaggccattatcgccggcatggcggccgacgcgctgggctacgtcttgctggcgttcgcgacgcgaggc tggatggccttccccattatgattcttctcgcttccggcggcatcgggatgcccgcgttgcaggccatgctgtccaggc aggtagatgacgaccatcagggacagcttcaaggatcgctcgcggctcttaccagcctaacttcgatcattggaccgct gatcgtcacggcgatttatgccgcctcggcgagcacatggaacgggttggcatggattgtaggcgccgccctatacctt gtctgcctccccgcgttgcgtcgcggtgcatggagccgggccacctcgacctaatactagagttatcaaaaaccatggt ttttgataa