##gff-version 3
##sequence-region BBa_J3103 1 1640
BBa_J3103	.	terminator	1515	1555	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_J3103	.	stem_loop	1522	1541	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J3103	.	stop_codon	1548	1548	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J3103	.	polyA_site	1542	1555	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J3103	.	engineered_region	1515	1555	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J3103	.	promoter	1	130	.	+	0	ID=BBa_I13453;Name=BBa_I13453
BBa_J3103	.	sequence_feature	1564	1640	.	+	0	ID=BBa_J3101;Name=BBa_J3101
BBa_J3103	.	sequence_feature	1564	1640	.	+	0	ID=annotation1884988;Name=RE Sequence;Parent=BBa_J3101
BBa_J3103	.	non_covalent_binding_site	1569	1584	.	+	0	ID=annotation1884991;Name=Proximal Fis Binding Site;Parent=BBa_J3101
BBa_J3103	.	sequence_feature	1614	1619	.	+	0	ID=annotation1880471;Name=Former SpeI site;Parent=BBa_J3101
BBa_J3103	.	sequence_feature	1592	1592	.	+	0	ID=annotation1884989;Name=Originally a C;Parent=BBa_J3101
BBa_J3103	.	non_covalent_binding_site	1618	1632	.	+	0	ID=annotation1884992;Name=Distal Fis Binding Site;Parent=BBa_J3101
BBa_J3103	.	sequence_feature	1614	1614	.	+	0	ID=annotation1880472;Name=Mutation of SpeI site;Parent=BBa_J3101
BBa_J3103	.	sequence_feature	1640	1640	.	+	0	ID=annotation1884990;Name=Insertion right before biobrick ends;Parent=BBa_J3101
BBa_J3103	.	terminator	1427	1506	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_J3103	.	stem_loop	1438	1481	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J3103	.	engineered_region	1427	1506	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J3103	.	sequence_feature	139	164	.	+	0	ID=BBa_J44000;Name=BBa_J44000
BBa_J3103	.	CDS	194	1384	.	+	0	ID=BBa_J31007;Name=BBa_J31007
BBa_J3103	.	primer_binding_site	1365	1384	.	+	0	ID=annotation1910572;Name=Rev primer J31007;Parent=BBa_J31007
BBa_J3103	.	primer_binding_site	194	217	.	+	0	ID=annotation1910571;Name=Fwd primer J31007;Parent=BBa_J31007
BBa_J3103	.	CDS	194	1384	.	+	0	ID=annotation1885000;Name=tetA(C) forward;Parent=BBa_J31007
BBa_J3103	.	stop_codon	1382	1384	.	+	0	ID=annotation1910573;Name=stop;Parent=BBa_J31007
BBa_J3103	.	sequence_feature	1393	1418	.	+	0	ID=BBa_J44000;Name=BBa_J44000
BBa_J3103	.	ribosome_entry_site	173	187	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_J3103	.	ribosome_entry_site	180	184	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_J3103	.	engineered_region	173	187	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_J3103	.	ribosome_entry_site	173	187	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
>BBa_J3103
acattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacg
ctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagttatcaaaaaccatggtttt
tgataatactagagattaaagaggagaaatactagatgaaatctaacaatgcgctcatcgtcatcctcggcaccgtcac
cctggatgctgtaggcataggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagcatc
gccagtcactatggcgtgctgctagcgctatatgcgttgatgcaatttctatgcgcacccgttctcggagcactgtccg
accgctttggccgccgcccagtcctgctcgcttcgctacttggagccactatcgactacgcgatcatggcgaccacacc
cgtcctgtggatcctctacgccggacgcatcgtggccggcatcaccggcgccacaggtgcggttgctggcgcctatatc
gccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcag
gccccgtggccgggggactgttgggcgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaa
cctactactgggctgcttcctaatgcaggagtcgcataagggagagcgtcgaccgatgcccttgagagccttcaaccca
gtcagctccttccggtgggcgcggggcatgactatcgtcgccgcacttatgactgtcttctttatcatgcaactcgtag
gacaggtgccggcagcgctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgct
tgcggtattcggaatcttgcacgccctcgctcaagccttcgtcactggccccgccaccaaacgtttcggcgagaagcag
gccattatcgccggcatggcggccgacgcgctgggctacgtcttgctggcgttcgcgacgcgaggctggatggccttcc
ccattatgattcttctcgcttccggcggcatcgggatgcccgcgttgcaggccatgctgtccaggcaggtagatgacga
ccatcagggacagcttcaaggatcgctcgcggctcttaccagcctaacttcgatcattggaccgctgatcgtcacggcg
atttatgccgcctcggcgagcacatggaacgggttggcatggattgtaggcgccgccctataccttgtctgcctccccg
cgttgcgtcgcggtgcatggagccgggccacctcgacctaatactagagttatcaaaaaccatggtttttgataatact
agagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacg
ctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagttcgggtgtcaacaatt
gaccaaaatattgatttacagcgtaatgcgctttctagtgcaaattgtgaccgcattttg