BBa_J3104
type: ComponentDefinition
persistent identity: BBa J3104
display id: BBa_J3104
version: 1
was generated by: iGEM to SBOL conversion
description: pBad-RBS-HixC-TetF-HixC-TT-RE
created: 2006-07-31T11:00:00Z
modified: 2015-08-31T04:08:45Z
mutable provenance: None
owned by: james
owned by: myers
mutable description: This part contains the gene for tetracycline resistance flanked by HixC regions. This part will be used to test if the ribosome will be able to read through the Hix site.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _61_
m user id: 0
owner id: 919
owning group id: 61
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: This part as well as part BBa_J3103 are going to be used to find the optimal placement of the RBS.
star: false
creator: Davidson and Missouri Western
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_J3101
component: BBa_B0012
component: BBa_B0010
component: BBa_J44000
component: BBa_J31007
component: BBa_B0030
component: BBa_I13453
sequence annotation: BBa_I13453
sequence annotation: BBa_B0030
sequence annotation: BBa_J44000
sequence annotation: BBa_J31007
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_J3101
sequence: https://synbiohub.org/public/igem/BBa_J3104_sequence/1

BBa_J3101
type: Component
persistent identity: component1892436
display id: component1892436
version: 1
top level: BBa_J3104
definition: RE
access: public

BBa_B0012
type: Component
persistent identity: component1892426
display id: component1892426
version: 1
top level: BBa_J3104
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1892424
display id: component1892424
version: 1
top level: BBa_J3104
definition: BBa_B0010
access: public

BBa_J44000
type: Component
persistent identity: component1892423
display id: component1892423
version: 1
top level: BBa_J3104
definition: hixC
access: public

BBa_J31007
type: Component
persistent identity: component1892422
display id: component1892422
version: 1
top level: BBa_J3104
definition: tetA(C)f
access: public

BBa_J44000
type: Component
persistent identity: component1892420
display id: component1892420
version: 1
top level: BBa_J3104
definition: hixC
access: public

BBa_B0030
type: Component
persistent identity: component1892418
display id: component1892418
version: 1
top level: BBa_J3104
definition: BBa_B0030
access: public

BBa_I13453
type: Component
persistent identity: component1892416
display id: component1892416
version: 1
top level: BBa_J3104
definition: BBa_I13453
access: public

BBa_I13453
type: SequenceAnnotation
persistent identity: annotation1892416
display id: annotation1892416
version: 1
top level: BBa_J3104
location: https://synbiohub.org/public/igem/BBa_J3104/annotation1892416/range1892416/1
component: BBa_I13453

https://synbiohub.org/public/igem/BBa_J3104/annotation1892416/range1892416/1
type: Range
persistent identity: range1892416
display id: range1892416
version: 1
top level: BBa_J3104
direction: direction/reverse
start: 1
end: 130
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation1892418
display id: annotation1892418
version: 1
top level: BBa_J3104
location: https://synbiohub.org/public/igem/BBa_J3104/annotation1892418/range1892418/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_J3104/annotation1892418/range1892418/1
type: Range
persistent identity: range1892418
display id: range1892418
version: 1
top level: BBa_J3104
direction: direction/forward
start: 139
end: 153
orientation: inline

BBa_J44000
type: SequenceAnnotation
persistent identity: annotation1892420
display id: annotation1892420
version: 1
top level: BBa_J3104
location: https://synbiohub.org/public/igem/BBa_J3104/annotation1892420/range1892420/1
component: BBa_J44000

https://synbiohub.org/public/igem/BBa_J3104/annotation1892420/range1892420/1
type: Range
persistent identity: range1892420
display id: range1892420
version: 1
top level: BBa_J3104
direction: direction/reverse
start: 162
end: 187
orientation: inline

BBa_J31007
type: SequenceAnnotation
persistent identity: annotation1892422
display id: annotation1892422
version: 1
top level: BBa_J3104
location: https://synbiohub.org/public/igem/BBa_J3104/annotation1892422/range1892422/1
component: BBa_J31007

https://synbiohub.org/public/igem/BBa_J3104/annotation1892422/range1892422/1
type: Range
persistent identity: range1892422
display id: range1892422
version: 1
top level: BBa_J3104
direction: direction/reverse
start: 194
end: 1384
orientation: inline

BBa_J44000
type: SequenceAnnotation
persistent identity: annotation1892423
display id: annotation1892423
version: 1
top level: BBa_J3104
location: https://synbiohub.org/public/igem/BBa_J3104/annotation1892423/range1892423/1
component: BBa_J44000

https://synbiohub.org/public/igem/BBa_J3104/annotation1892423/range1892423/1
type: Range
persistent identity: range1892423
display id: range1892423
version: 1
top level: BBa_J3104
direction: direction/reverse
start: 1393
end: 1418
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1892424
display id: annotation1892424
version: 1
top level: BBa_J3104
location: https://synbiohub.org/public/igem/BBa_J3104/annotation1892424/range1892424/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J3104/annotation1892424/range1892424/1
type: Range
persistent identity: range1892424
display id: range1892424
version: 1
top level: BBa_J3104
direction: direction/forward
start: 1427
end: 1506
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1892426
display id: annotation1892426
version: 1
top level: BBa_J3104
location: https://synbiohub.org/public/igem/BBa_J3104/annotation1892426/range1892426/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J3104/annotation1892426/range1892426/1
type: Range
persistent identity: range1892426
display id: range1892426
version: 1
top level: BBa_J3104
direction: direction/forward
start: 1515
end: 1555
orientation: inline

BBa_J3101
type: SequenceAnnotation
persistent identity: annotation1892436
display id: annotation1892436
version: 1
top level: BBa_J3104
location: https://synbiohub.org/public/igem/BBa_J3104/annotation1892436/range1892436/1
component: BBa_J3101

https://synbiohub.org/public/igem/BBa_J3104/annotation1892436/range1892436/1
type: Range
persistent identity: range1892436
display id: range1892436
version: 1
top level: BBa_J3104
direction: direction/reverse
start: 1564
end: 1640
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

BBa_B0030
type: ComponentDefinition
persistent identity: BBa B0030
display id: BBa_B0030
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.1 (strong) -- modified from R. Weiss
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _44_46_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: RBS-1\Strong
sequence annotation: BBa_B0030
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0030_sequence/1

RBS-1\Strong
type: SequenceAnnotation
persistent identity: annotation1701
display id: annotation1701
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
type: Range
persistent identity: range1701
display id: range1701
version: 1
top level: BBa_B0030
direction: direction/forward
start: 1
end: 15
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation7025
display id: annotation7025
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
type: Range
persistent identity: range7025
display id: range7025
version: 1
top level: BBa_B0030
direction: direction/forward
start: 1
end: 15
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1702
display id: annotation1702
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
type: Range
persistent identity: range1702
display id: range1702
version: 1
top level: BBa_B0030
direction: direction/forward
start: 8
end: 12
orientation: inline

hixC
type: ComponentDefinition
persistent identity: BBa J44000
display id: BBa_J44000
version: 1
was generated by: iGEM to SBOL conversion
description: hixC binding site for Salmonella typhimurium Hin recombinase
created: 2006-06-05T11:00:00Z
modified: 2015-08-31T04:08:48Z
mutable provenance: Nanassy and Hughes. 1998. In Vivo Identification of Intermediate Stages of the DNA Inversion Reaction Catlyzed by the Salmonella Hin Recombinase [http://www.genetics.org/cgi/content/abstract/149/4/1649]
owned by: james
owned by: myers
mutable description: A 26 bp sequence of DNA composed of 12 bp inverted repeats and a 2 bp core that operates in Salmonella paired with a hixR binding site to recombine DNA. A second hix site is required for recombination to occur. The two sites bind Hin recombinase in the formation of an invertasome.
discontinued: false
dominant: true
experience: experience/Works
group u list: _71_
m user id: 0
owner id: 606
owning group id: 61
sample status: In stock
status: status/Available
bookmark: true
mutable notes: Standard BioBrick prefix and suffix were added to the 26 bp sequence.
star: true
creator: Missouri Western and Davidson Groups, Todd Eckdahl
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_J44000_sequence/1

BBa_I13453
type: ComponentDefinition
persistent identity: BBa I13453
display id: BBa_I13453
version: 1
was generated by: iGEM to SBOL conversion
description: Pbad promoter
created: 2005-05-24T11:00:00Z
modified: 2015-08-31T04:07:34Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: PBad promoter from I0500 without AraC.
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_
m user id: 0
owner id: 253
owning group id: 6
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: jkm
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I13453_sequence/1

tetA(C)f
type: ComponentDefinition
persistent identity: BBa J31007
display id: BBa_J31007
version: 1
was generated by: iGEM to SBOL conversion
description: tetracycline resistance protein TetA(C) (forward), [cf. BBa_J31006]
created: 2006-07-11T11:00:00Z
modified: 2015-08-31T04:08:45Z
mutable provenance: PsB1AT3
owned by: james
owned by: myers
mutable description: This part is the coding region of the TetR gene. It requires a promoter and RBS for the cell to be able to express tetracyline resistance.
discontinued: false
dominant: false
experience: experience/Works
group u list: _61_
m user id: 0
owner id: 919
owning group id: 61
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: It was cloned into PsB1A2 plamsid.
star: true
creator: Sabriya Rosemond, Erin Zwack
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: tetA(C) forward
sequence annotation: Fwd primer J31007
sequence annotation: Rev primer J31007
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_J31007_sequence/1

tetA(C) forward
type: SequenceAnnotation
persistent identity: annotation1885000
display id: annotation1885000
version: 1
top level: tetA(C)f
location: https://synbiohub.org/public/igem/BBa_J31007/annotation1885000/range1885000/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_J31007/annotation1885000/range1885000/1
type: Range
persistent identity: range1885000
display id: range1885000
version: 1
top level: tetA(C)f
direction: direction/forward
start: 1
end: 1191
orientation: inline

Fwd primer J31007
type: SequenceAnnotation
persistent identity: annotation1910571
display id: annotation1910571
version: 1
top level: tetA(C)f
location: https://synbiohub.org/public/igem/BBa_J31007/annotation1910571/range1910571/1
role: feature/primer binding
role: SO:0005850

https://synbiohub.org/public/igem/BBa_J31007/annotation1910571/range1910571/1
type: Range
persistent identity: range1910571
display id: range1910571
version: 1
top level: tetA(C)f
direction: direction/forward
start: 1
end: 24
orientation: inline

Rev primer J31007
type: SequenceAnnotation
persistent identity: annotation1910572
display id: annotation1910572
version: 1
top level: tetA(C)f
location: https://synbiohub.org/public/igem/BBa_J31007/annotation1910572/range1910572/1
role: feature/primer binding
role: SO:0005850

https://synbiohub.org/public/igem/BBa_J31007/annotation1910572/range1910572/1
type: Range
persistent identity: range1910572
display id: range1910572
version: 1
top level: tetA(C)f
direction: direction/reverse
start: 1172
end: 1191
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1910573
display id: annotation1910573
version: 1
top level: tetA(C)f
location: https://synbiohub.org/public/igem/BBa_J31007/annotation1910573/range1910573/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_J31007/annotation1910573/range1910573/1
type: Range
persistent identity: range1910573
display id: range1910573
version: 1
top level: tetA(C)f
direction: direction/forward
start: 1189
end: 1191
orientation: inline

RE
type: ComponentDefinition
persistent identity: BBa J3101
display id: BBa_J3101
version: 1
was generated by: iGEM to SBOL conversion
description: Recombinational Enhancer (RE) for Hin/Hix inverting
created: 2006-06-01T11:00:00Z
modified: 2015-08-31T04:08:45Z
owned by: james
owned by: myers
discontinued: false
dominant: false
experience: experience/Works
group u list: _61_
m user id: 0
owner id: 918
owning group id: 61
sample status: In stock
status: status/Available
bookmark: true
star: true
creator: Erin Zwack, Sabriya Rosemond
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence annotation: RE Sequence
sequence annotation: Proximal Fis Binding Site
sequence annotation: Originally a C
sequence annotation: Former SpeI site
sequence annotation: Mutation of SpeI site
sequence annotation: Distal Fis Binding Site
sequence annotation: Insertion right before biobrick ends
sequence: https://synbiohub.org/public/igem/BBa_J3101_sequence/1

RE Sequence
type: SequenceAnnotation
persistent identity: annotation1884988
display id: annotation1884988
version: 1
top level: RE
location: https://synbiohub.org/public/igem/BBa_J3101/annotation1884988/range1884988/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_J3101/annotation1884988/range1884988/1
type: Range
persistent identity: range1884988
display id: range1884988
version: 1
top level: RE
direction: direction/forward
start: 1
end: 77
orientation: inline

Proximal Fis Binding Site
type: SequenceAnnotation
persistent identity: annotation1884991
display id: annotation1884991
version: 1
top level: RE
location: https://synbiohub.org/public/igem/BBa_J3101/annotation1884991/range1884991/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_J3101/annotation1884991/range1884991/1
type: Range
persistent identity: range1884991
display id: range1884991
version: 1
top level: RE
direction: direction/forward
start: 6
end: 21
orientation: inline

Originally a C
type: SequenceAnnotation
persistent identity: annotation1884989
display id: annotation1884989
version: 1
top level: RE
location: https://synbiohub.org/public/igem/BBa_J3101/annotation1884989/range1884989/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_J3101/annotation1884989/range1884989/1
type: Range
persistent identity: range1884989
display id: range1884989
version: 1
top level: RE
direction: direction/forward
start: 29
end: 29
orientation: inline

Former SpeI site
type: SequenceAnnotation
persistent identity: annotation1880471
display id: annotation1880471
version: 1
top level: RE
location: https://synbiohub.org/public/igem/BBa_J3101/annotation1880471/range1880471/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_J3101/annotation1880471/range1880471/1
type: Range
persistent identity: range1880471
display id: range1880471
version: 1
top level: RE
direction: direction/forward
start: 51
end: 56
orientation: inline

Mutation of SpeI site
type: SequenceAnnotation
persistent identity: annotation1880472
display id: annotation1880472
version: 1
top level: RE
location: https://synbiohub.org/public/igem/BBa_J3101/annotation1880472/range1880472/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_J3101/annotation1880472/range1880472/1
type: Range
persistent identity: range1880472
display id: range1880472
version: 1
top level: RE
direction: direction/forward
start: 51
end: 51
orientation: inline

Distal Fis Binding Site
type: SequenceAnnotation
persistent identity: annotation1884992
display id: annotation1884992
version: 1
top level: RE
location: https://synbiohub.org/public/igem/BBa_J3101/annotation1884992/range1884992/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_J3101/annotation1884992/range1884992/1
type: Range
persistent identity: range1884992
display id: range1884992
version: 1
top level: RE
direction: direction/forward
start: 55
end: 69
orientation: inline

Insertion right before biobrick ends
type: SequenceAnnotation
persistent identity: annotation1884990
display id: annotation1884990
version: 1
top level: RE
location: https://synbiohub.org/public/igem/BBa_J3101/annotation1884990/range1884990/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_J3101/annotation1884990/range1884990/1
type: Range
persistent identity: range1884990
display id: range1884990
version: 1
top level: RE
direction: direction/forward
start: 77
end: 77
orientation: inline

https://synbiohub.org/public/igem/BBa_J3104_sequence/1
type: Sequence
persistent identity: BBa J3104 sequence
display id: BBa_J3104_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagattaaagaggagaaatactagagttatcaaaaaccatggtttttgataatactagatgaaatctaacaatgcgctcatcgtcatcctcggcaccgtcaccctggatgctgtaggcataggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagcatcgccagtcactatggcgtgctgctagcgctatatgcgttgatgcaatttctatgcgcacccgttctcggagcactgtccgaccgctttggccgccgcccagtcctgctcgcttcgctacttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggatcctctacgccggacgcatcgtggccggcatcaccggcgccacaggtgcggttgctggcgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttgggcgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaacctactactgggctgcttcctaatgcaggagtcgcataagggagagcgtcgaccgatgcccttgagagccttcaacccagtcagctccttccggtgggcgcggggcatgactatcgtcgccgcacttatgactgtcttctttatcatgcaactcgtaggacaggtgccggcagcgctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatcttgcacgccctcgctcaagccttcgtcactggccccgccaccaaacgtttcggcgagaagcaggccattatcgccggcatggcggccgacgcgctgggctacgtcttgctggcgttcgcgacgcgaggctggatggccttccccattatgattcttctcgcttccggcggcatcgggatgcccgcgttgcaggccatgctgtccaggcaggtagatgacgaccatcagggacagcttcaaggatcgctcgcggctcttaccagcctaacttcgatcattggaccgctgatcgtcacggcgatttatgccgcctcggcgagcacatggaacgggttggcatggattgtaggcgccgccctataccttgtctgcctccccgcgttgcgtcgcggtgcatggagccgggccacctcgacctaatactagagttatcaaaaaccatggtttttgataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagttcgggtgtcaacaattgaccaaaatattgatttacagcgtaatgcgctttctagtgcaaattgtgaccgcattttg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0030_sequence/1
type: Sequence
persistent identity: BBa B0030 sequence
display id: BBa_B0030_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attaaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J44000_sequence/1
type: Sequence
persistent identity: BBa J44000 sequence
display id: BBa_J44000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttatcaaaaaccatggtttttgataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I13453_sequence/1
type: Sequence
persistent identity: BBa I13453 sequence
display id: BBa_I13453_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J31007_sequence/1
type: Sequence
persistent identity: BBa J31007 sequence
display id: BBa_J31007_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaaatctaacaatgcgctcatcgtcatcctcggcaccgtcaccctggatgctgtaggcataggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagcatcgccagtcactatggcgtgctgctagcgctatatgcgttgatgcaatttctatgcgcacccgttctcggagcactgtccgaccgctttggccgccgcccagtcctgctcgcttcgctacttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggatcctctacgccggacgcatcgtggccggcatcaccggcgccacaggtgcggttgctggcgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttgggcgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaacctactactgggctgcttcctaatgcaggagtcgcataagggagagcgtcgaccgatgcccttgagagccttcaacccagtcagctccttccggtgggcgcggggcatgactatcgtcgccgcacttatgactgtcttctttatcatgcaactcgtaggacaggtgccggcagcgctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatcttgcacgccctcgctcaagccttcgtcactggccccgccaccaaacgtttcggcgagaagcaggccattatcgccggcatggcggccgacgcgctgggctacgtcttgctggcgttcgcgacgcgaggctggatggccttccccattatgattcttctcgcttccggcggcatcgggatgcccgcgttgcaggccatgctgtccaggcaggtagatgacgaccatcagggacagcttcaaggatcgctcgcggctcttaccagcctaacttcgatcattggaccgctgatcgtcacggcgatttatgccgcctcggcgagcacatggaacgggttggcatggattgtaggcgccgccctataccttgtctgcctccccgcgttgcgtcgcggtgcatggagccgggccacctcgacctaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J3101_sequence/1
type: Sequence
persistent identity: BBa J3101 sequence
display id: BBa_J3101_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttcgggtgtcaacaattgaccaaaatattgatttacagcgtaatgcgctttctagtgcaaattgtgaccgcattttg
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z