LOCUS       BBa_J3105               1642 bp    DNA     linear       16-Jun-2024
DEFINITION  pBad-RBS-HixC-TetB-HixC-TT-RE
ACCESSION   BBa_J3105
VERSION     BBa_J3105.1  
FEATURES             Location/Qualifiers
     promoter        1..130
                     /label=BBa_I13453
     RBS             139..153
                     /label=RBS-1\Strong
     RBS             146..150
                     /label=RBS
     misc_feature    139..153
                     /label=BBa_B0030
     RBS             139..153
                     /label=BBa_B0030
     promoter        1..153
                     /label=BBa_J3102
     misc_feature    162..187
                     /label=BBa_J44000
     CDS             196..1386
                     /label=tetA(C) backwards
     misc_feature    196..198
                     /label=stop
     primer_bind     196..215
                     /label=Rev primer J31006
     primer_bind     1363..1386
                     /label=Fwd primer J31006
     CDS             196..1386
                     /label=BBa_J31006
     misc_feature    1395..1420
                     /label=BBa_J44000
     misc_feature    196..1420
                     /label=BBa_S03525
     stem_loop       1440..1483
                     /label=stem_loop
     misc_feature    1429..1508
                     /label=BBa_B0010
     terminator      1429..1508
                     /label=BBa_B0010
     stem_loop       1524..1543
                     /label=T7 TE
     misc_feature    1550..1550
                     /label=stop
     polyA_site      1544..1557
                     /label=polya
     misc_feature    1517..1557
                     /label=BBa_B0012
     terminator      1517..1557
                     /label=BBa_B0012
     misc_feature    1616..1621
                     /label=Former SpeI site
     misc_feature    1616..1616
                     /label=Mutation of SpeI site
     misc_feature    1566..1642
                     /label=RE Sequence
     misc_feature    1594..1594
                     /label=Originally a C
     misc_feature    1642..1642
                     /label=Insertion right before biobrick ends
     misc_feature    1571..1586
                     /label=Proximal Fis Binding Site
     misc_feature    1620..1634
                     /label=Distal Fis Binding Site
     misc_feature    1566..1642
                     /label=BBa_J3101
     misc_feature    1429..1642
                     /label=BBa_S03516
     misc_feature    196..1642
                     /label=BBa_S03526
     misc_feature    162..1642
                     /label=BBa_S03528
ORIGIN
        1 acattgatta tttgcacggc gtcacacttt gctatgccat agcattttta tccataagat
       61 tagcggatcc tacctgacgc tttttatcgc aactctctac tgtttctcca taccgttttt
      121 ttgggctagc tactagagat taaagaggag aaatactaga gttatcaaaa accatggttt
      181 ttgataatac tagagttagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg
      241 gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc
      301 cgaggcggca taaatcgccg tgacgatcag cggtccaatg atcgaagtta ggctggtaag
      361 agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat
      421 ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc
      481 catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc
      541 ggcgataatg gcctgcttct cgccgaaacg tttggtggcg gggccagtga cgaaggcttg
      601 agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca
      661 gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg
      721 catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa
      781 ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc
      841 ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa
      901 tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc
      961 cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat
     1021 gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg
     1081 tccggcgtag aggatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc
     1141 tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag
     1201 aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact
     1261 ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa
     1321 gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga
     1381 tttcattact agagttatca aaaaccatgg tttttgataa tactagagcc aggcatcaaa
     1441 taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga
     1501 acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttatatac
     1561 tagagttcgg gtgtcaacaa ttgaccaaaa tattgattta cagcgtaatg cgctttctag
     1621 tgcaaattgt gaccgcattt tg
//