##gff-version 3
##sequence-region BBa_J3105 1 1642
BBa_J3105	.	promoter	1	153	.	+	0	ID=BBa_J3102;Name=BBa_J3102
BBa_J3105	.	promoter	1	130	.	+	0	ID=BBa_I13453;Name=BBa_I13453;Parent=BBa_J3102
BBa_J3105	.	ribosome_entry_site	139	153	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_J3102
BBa_J3105	.	ribosome_entry_site	139	153	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_J3105	.	engineered_region	139	153	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_J3105	.	ribosome_entry_site	146	150	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_J3105	.	engineered_region	162	1642	.	+	0	ID=BBa_S03528;Name=BBa_S03528
BBa_J3105	.	sequence_feature	162	187	.	+	0	ID=BBa_J44000;Name=BBa_J44000;Parent=BBa_S03528
BBa_J3105	.	engineered_region	196	1642	.	+	0	ID=BBa_S03526;Name=BBa_S03526;Parent=BBa_S03528
BBa_J3105	.	engineered_region	196	1420	.	+	0	ID=BBa_S03525;Name=BBa_S03525;Parent=BBa_S03526
BBa_J3105	.	sequence_feature	1395	1420	.	+	0	ID=BBa_J44000;Name=BBa_J44000;Parent=BBa_S03525
BBa_J3105	.	CDS	196	1386	.	+	0	ID=BBa_J31006;Name=BBa_J31006;Parent=BBa_S03525
BBa_J3105	.	CDS	196	1386	.	+	0	ID=annotation1884998;Name=tetA(C) backwards;Parent=BBa_J31006
BBa_J3105	.	primer_binding_site	1363	1386	.	+	0	ID=annotation1910576;Name=Fwd primer J31006;Parent=BBa_J31006
BBa_J3105	.	primer_binding_site	196	215	.	+	0	ID=annotation1910575;Name=Rev primer J31006;Parent=BBa_J31006
BBa_J3105	.	stop_codon	196	198	.	+	0	ID=annotation1910574;Name=stop;Parent=BBa_J31006
BBa_J3105	.	engineered_region	1429	1642	.	+	0	ID=BBa_S03516;Name=BBa_S03516;Parent=BBa_S03526
BBa_J3105	.	sequence_feature	1566	1642	.	+	0	ID=BBa_J3101;Name=BBa_J3101;Parent=BBa_S03516
BBa_J3105	.	non_covalent_binding_site	1571	1586	.	+	0	ID=annotation1884991;Name=Proximal Fis Binding Site;Parent=BBa_J3101
BBa_J3105	.	sequence_feature	1616	1621	.	+	0	ID=annotation1880471;Name=Former SpeI site;Parent=BBa_J3101
BBa_J3105	.	sequence_feature	1616	1616	.	+	0	ID=annotation1880472;Name=Mutation of SpeI site;Parent=BBa_J3101
BBa_J3105	.	non_covalent_binding_site	1620	1634	.	+	0	ID=annotation1884992;Name=Distal Fis Binding Site;Parent=BBa_J3101
BBa_J3105	.	sequence_feature	1566	1642	.	+	0	ID=annotation1884988;Name=RE Sequence;Parent=BBa_J3101
BBa_J3105	.	sequence_feature	1594	1594	.	+	0	ID=annotation1884989;Name=Originally a C;Parent=BBa_J3101
BBa_J3105	.	sequence_feature	1642	1642	.	+	0	ID=annotation1884990;Name=Insertion right before biobrick ends;Parent=BBa_J3101
BBa_J3105	.	terminator	1429	1508	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_S03516
BBa_J3105	.	engineered_region	1429	1508	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J3105	.	stem_loop	1440	1483	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J3105	.	terminator	1517	1557	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_S03516
BBa_J3105	.	engineered_region	1517	1557	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J3105	.	stem_loop	1524	1543	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J3105	.	polyA_site	1544	1557	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J3105	.	stop_codon	1550	1550	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
>BBa_J3105
acattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacg
ctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagattaaagaggagaaatacta
gagttatcaaaaaccatggtttttgataatactagagttaggtcgaggtggcccggctccatgcaccgcgacgcaacgc
ggggaggcagacaaggtatagggcggcgcctacaatccatgccaacccgttccatgtgctcgccgaggcggcataaatc
gccgtgacgatcagcggtccaatgatcgaagttaggctggtaagagccgcgagcgatccttgaagctgtccctgatggt
cgtcatctacctgcctggacagcatggcctgcaacgcgggcatcccgatgccgccggaagcgagaagaatcataatggg
gaaggccatccagcctcgcgtcgcgaacgccagcaagacgtagcccagcgcgtcggccgccatgccggcgataatggcc
tgcttctcgccgaaacgtttggtggcggggccagtgacgaaggcttgagcgagggcgtgcaagattccgaataccgcaa
gcgacaggccgatcatcgtcgcgctccagcgaaagcggtcctcgccgaaaatgacccagagcgctgccggcacctgtcc
tacgagttgcatgataaagaagacagtcataagtgcggcgacgatagtcatgccccgcgcccaccggaaggagctgact
gggttgaaggctctcaagggcatcggtcgacgctctcccttatgcgactcctgcattaggaagcagcccagtagtaggt
tgaggccgttgagcaccgccgccgcaaggaatggtgcatgcaaggagatggcgcccaacagtcccccggccacggggcc
tgccaccatacccacgccgaaacaagcgctcatgagcccgaagtggcgagcccgatcttccccatcggtgatgtcggcg
atataggcgccagcaaccgcacctgtggcgccggtgatgccggccacgatgcgtccggcgtagaggatccacaggacgg
gtgtggtcgccatgatcgcgtagtcgatagtggctccaagtagcgaagcgagcaggactgggcggcggccaaagcggtc
ggacagtgctccgagaacgggtgcgcatagaaattgcatcaacgcatatagcgctagcagcacgccatagtgactggcg
atgctgtcggaatggacgatatcccgcaagaggcccggcagtaccggcataaccaagcctatgcctacagcatccaggg
tgacggtgccgaggatgacgatgagcgcattgttagatttcattactagagttatcaaaaaccatggtttttgataata
ctagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaa
cgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagttcgggtgtcaacaa
ttgaccaaaatattgatttacagcgtaatgcgctttctagtgcaaattgtgaccgcattttg