##gff-version 3
##sequence-region BBa_J3106 1 1608
BBa_J3106	.	engineered_region	1395	1608	.	+	0	ID=BBa_S03516;Name=BBa_S03516
BBa_J3106	.	sequence_feature	1532	1608	.	+	0	ID=BBa_J3101;Name=BBa_J3101;Parent=BBa_S03516
BBa_J3106	.	non_covalent_binding_site	1586	1600	.	+	0	ID=annotation1884992;Name=Distal Fis Binding Site;Parent=BBa_J3101
BBa_J3106	.	sequence_feature	1608	1608	.	+	0	ID=annotation1884990;Name=Insertion right before biobrick ends;Parent=BBa_J3101
BBa_J3106	.	non_covalent_binding_site	1537	1552	.	+	0	ID=annotation1884991;Name=Proximal Fis Binding Site;Parent=BBa_J3101
BBa_J3106	.	sequence_feature	1582	1582	.	+	0	ID=annotation1880472;Name=Mutation of SpeI site;Parent=BBa_J3101
BBa_J3106	.	sequence_feature	1532	1608	.	+	0	ID=annotation1884988;Name=RE Sequence;Parent=BBa_J3101
BBa_J3106	.	sequence_feature	1560	1560	.	+	0	ID=annotation1884989;Name=Originally a C;Parent=BBa_J3101
BBa_J3106	.	sequence_feature	1582	1587	.	+	0	ID=annotation1880471;Name=Former SpeI site;Parent=BBa_J3101
BBa_J3106	.	terminator	1483	1523	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_S03516
BBa_J3106	.	engineered_region	1483	1523	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J3106	.	stem_loop	1490	1509	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J3106	.	stop_codon	1516	1516	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J3106	.	polyA_site	1510	1523	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J3106	.	terminator	1395	1474	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_S03516
BBa_J3106	.	engineered_region	1395	1474	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J3106	.	stem_loop	1406	1449	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J3106	.	engineered_region	1	1386	.	+	0	ID=BBa_S03533;Name=BBa_S03533
BBa_J3106	.	engineered_region	1	164	.	+	0	ID=BBa_S03529;Name=BBa_S03529;Parent=BBa_S03533
BBa_J3106	.	sequence_feature	139	164	.	+	0	ID=BBa_J44000;Name=BBa_J44000;Parent=BBa_S03529
BBa_J3106	.	promoter	1	130	.	+	0	ID=BBa_I13453;Name=BBa_I13453;Parent=BBa_S03529
BBa_J3106	.	engineered_region	173	1386	.	+	0	ID=BBa_S03532;Name=BBa_S03532;Parent=BBa_S03533
BBa_J3106	.	CDS	173	1363	.	+	0	ID=BBa_J31006;Name=BBa_J31006;Parent=BBa_S03532
BBa_J3106	.	CDS	173	1363	.	+	0	ID=annotation1884998;Name=tetA(C) backwards;Parent=BBa_J31006
BBa_J3106	.	primer_binding_site	1340	1363	.	+	0	ID=annotation1910576;Name=Fwd primer J31006;Parent=BBa_J31006
BBa_J3106	.	primer_binding_site	173	192	.	+	0	ID=annotation1910575;Name=Rev primer J31006;Parent=BBa_J31006
BBa_J3106	.	stop_codon	173	175	.	+	0	ID=annotation1910574;Name=stop;Parent=BBa_J31006
BBa_J3106	.	ribosome_entry_site	1372	1386	.	+	0	ID=BBa_J44001;Name=BBa_J44001;Parent=BBa_S03532
BBa_J3106	.	ribosome_entry_site	1372	1386	.	+	0	ID=annotation1893199;Name=Reverse RBS;Parent=BBa_J44001
>BBa_J3106
acattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacg
ctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagttatcaaaaaccatggtttt
tgataatactagagttaggtcgaggtggcccggctccatgcaccgcgacgcaacgcggggaggcagacaaggtataggg
cggcgcctacaatccatgccaacccgttccatgtgctcgccgaggcggcataaatcgccgtgacgatcagcggtccaat
gatcgaagttaggctggtaagagccgcgagcgatccttgaagctgtccctgatggtcgtcatctacctgcctggacagc
atggcctgcaacgcgggcatcccgatgccgccggaagcgagaagaatcataatggggaaggccatccagcctcgcgtcg
cgaacgccagcaagacgtagcccagcgcgtcggccgccatgccggcgataatggcctgcttctcgccgaaacgtttggt
ggcggggccagtgacgaaggcttgagcgagggcgtgcaagattccgaataccgcaagcgacaggccgatcatcgtcgcg
ctccagcgaaagcggtcctcgccgaaaatgacccagagcgctgccggcacctgtcctacgagttgcatgataaagaaga
cagtcataagtgcggcgacgatagtcatgccccgcgcccaccggaaggagctgactgggttgaaggctctcaagggcat
cggtcgacgctctcccttatgcgactcctgcattaggaagcagcccagtagtaggttgaggccgttgagcaccgccgcc
gcaaggaatggtgcatgcaaggagatggcgcccaacagtcccccggccacggggcctgccaccatacccacgccgaaac
aagcgctcatgagcccgaagtggcgagcccgatcttccccatcggtgatgtcggcgatataggcgccagcaaccgcacc
tgtggcgccggtgatgccggccacgatgcgtccggcgtagaggatccacaggacgggtgtggtcgccatgatcgcgtag
tcgatagtggctccaagtagcgaagcgagcaggactgggcggcggccaaagcggtcggacagtgctccgagaacgggtg
cgcatagaaattgcatcaacgcatatagcgctagcagcacgccatagtgactggcgatgctgtcggaatggacgatatc
ccgcaagaggcccggcagtaccggcataaccaagcctatgcctacagcatccagggtgacggtgccgaggatgacgatg
agcgcattgttagatttcattactagagtttctcctctttaattactagagccaggcatcaaataaaacgaaaggctca
gtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcg
ggtgggcctttctgcgtttatatactagagttcgggtgtcaacaattgaccaaaatattgatttacagcgtaatgcgct
ttctagtgcaaattgtgaccgcattttg