BBa_J3107
type: ComponentDefinition
persistent identity: BBa J3107
display id: BBa_J3107
version: 1
was generated by: iGEM to SBOL conversion
description: AraC-PC, pLac-Hin
created: 2006-10-11T11:00:00Z
modified: 2015-08-31T04:08:45Z
mutable provenance: AraC-PC-TT - part BBa_S03591, a composite of BBa_I13458 and part BBa_B0015 pLac-RBS-Hin-TT - part BBa_S03537
owned by: james
owned by: myers
mutable description: AraC-PC (part BBa_I13458) should constitutively express the AraC protein (inhibitor of the pBad promoter). Expression of Hin invertase is under control of the pLac promoter. Therefore, Hin should be expressed in the presence of IPTG. This part was designed for two reasons: 1. To regulate DNA inversion (via controlled Hin invertase expression) on a second plasmid 2. To regulate the transcription of a pBad driven reporter (via the addition of arabinose) on the second plasmid
discontinued: false
dominant: false
experience: experience/Works
group u list: _61_
m user id: 0
owner id: 1144
owning group id: 61
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: A double forward terminator is placed upstream of "pLac-Hin" to block forward read-through transcription from the pSB1AK3 vector. This should allow tighter control over Hin expression.
star: false
creator: Karmella Haynes
type: DnaRegion
role: partType/Generator
role: SO:0000804
component: BBa_B0010
component: BBa_I13458
component: BBa_B0012
component: BBa_R0010
component: BBa_J31000
component: BBa_B0030
sequence annotation: BBa_I13458
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_R0010
sequence annotation: BBa_B0030
sequence annotation: BBa_J31000
sequence: https://synbiohub.org/public/igem/BBa_J3107_sequence/1

BBa_B0010
type: Component
persistent identity: component1902758
display id: component1902758
version: 1
top level: BBa_J3107
definition: BBa_B0010
access: public

BBa_I13458
type: Component
persistent identity: component1902757
display id: component1902757
version: 1
top level: BBa_J3107
definition: BBa_I13458
access: public

BBa_B0012
type: Component
persistent identity: component1902780
display id: component1902780
version: 1
top level: BBa_J3107
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1902778
display id: component1902778
version: 1
top level: BBa_J3107
definition: BBa_B0010
access: public

BBa_R0010
type: Component
persistent identity: component1902764
display id: component1902764
version: 1
top level: BBa_J3107
definition: LacI
access: public

BBa_B0012
type: Component
persistent identity: component1902760
display id: component1902760
version: 1
top level: BBa_J3107
definition: BBa_B0012
access: public

BBa_J31000
type: Component
persistent identity: component1902777
display id: component1902777
version: 1
top level: BBa_J3107
definition: Hin
access: public

BBa_B0030
type: Component
persistent identity: component1902772
display id: component1902772
version: 1
top level: BBa_J3107
definition: BBa_B0030
access: public

BBa_I13458
type: SequenceAnnotation
persistent identity: annotation1902757
display id: annotation1902757
version: 1
top level: BBa_J3107
location: https://synbiohub.org/public/igem/BBa_J3107/annotation1902757/range1902757/1
component: BBa_I13458

https://synbiohub.org/public/igem/BBa_J3107/annotation1902757/range1902757/1
type: Range
persistent identity: range1902757
display id: range1902757
version: 1
top level: BBa_J3107
direction: direction/reverse
start: 1
end: 1114
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1902758
display id: annotation1902758
version: 1
top level: BBa_J3107
location: https://synbiohub.org/public/igem/BBa_J3107/annotation1902758/range1902758/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J3107/annotation1902758/range1902758/1
type: Range
persistent identity: range1902758
display id: range1902758
version: 1
top level: BBa_J3107
direction: direction/forward
start: 1123
end: 1202
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1902760
display id: annotation1902760
version: 1
top level: BBa_J3107
location: https://synbiohub.org/public/igem/BBa_J3107/annotation1902760/range1902760/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J3107/annotation1902760/range1902760/1
type: Range
persistent identity: range1902760
display id: range1902760
version: 1
top level: BBa_J3107
direction: direction/forward
start: 1211
end: 1251
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation1902764
display id: annotation1902764
version: 1
top level: BBa_J3107
location: https://synbiohub.org/public/igem/BBa_J3107/annotation1902764/range1902764/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_J3107/annotation1902764/range1902764/1
type: Range
persistent identity: range1902764
display id: range1902764
version: 1
top level: BBa_J3107
direction: direction/forward
start: 1260
end: 1459
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation1902772
display id: annotation1902772
version: 1
top level: BBa_J3107
location: https://synbiohub.org/public/igem/BBa_J3107/annotation1902772/range1902772/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_J3107/annotation1902772/range1902772/1
type: Range
persistent identity: range1902772
display id: range1902772
version: 1
top level: BBa_J3107
direction: direction/forward
start: 1468
end: 1482
orientation: inline

BBa_J31000
type: SequenceAnnotation
persistent identity: annotation1902777
display id: annotation1902777
version: 1
top level: BBa_J3107
location: https://synbiohub.org/public/igem/BBa_J3107/annotation1902777/range1902777/1
component: BBa_J31000

https://synbiohub.org/public/igem/BBa_J3107/annotation1902777/range1902777/1
type: Range
persistent identity: range1902777
display id: range1902777
version: 1
top level: BBa_J3107
direction: direction/reverse
start: 1489
end: 2061
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1902778
display id: annotation1902778
version: 1
top level: BBa_J3107
location: https://synbiohub.org/public/igem/BBa_J3107/annotation1902778/range1902778/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J3107/annotation1902778/range1902778/1
type: Range
persistent identity: range1902778
display id: range1902778
version: 1
top level: BBa_J3107
direction: direction/forward
start: 2070
end: 2149
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1902780
display id: annotation1902780
version: 1
top level: BBa_J3107
location: https://synbiohub.org/public/igem/BBa_J3107/annotation1902780/range1902780/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J3107/annotation1902780/range1902780/1
type: Range
persistent identity: range1902780
display id: range1902780
version: 1
top level: BBa_J3107
direction: direction/forward
start: 2158
end: 2198
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

LacI
type: ComponentDefinition
persistent identity: BBa R0010
display id: BBa_R0010
version: 1
was generated by: iGEM to SBOL conversion
description: promoter (lacI regulated)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: The Plac insert was PCR'd from the MG1655 strain of E.coli K12.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs.
star: true
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: end of LacI coding region (inactive)
sequence annotation: BBa_R0010
sequence annotation: CAP binding site
sequence annotation: -35
sequence annotation: -10
sequence annotation: LacI binding site
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_R0010_sequence/1

end of LacI coding region (inactive)
type: SequenceAnnotation
persistent identity: annotation1961221
display id: annotation1961221
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
type: Range
persistent identity: range1961221
display id: range1961221
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 88
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation1961222
display id: annotation1961222
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
type: Range
persistent identity: range1961222
display id: range1961222
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 200
orientation: inline

CAP binding site
type: SequenceAnnotation
persistent identity: annotation1961223
display id: annotation1961223
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
type: Range
persistent identity: range1961223
display id: range1961223
version: 1
top level: LacI
direction: direction/forward
start: 89
end: 126
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1961224
display id: annotation1961224
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
type: Range
persistent identity: range1961224
display id: range1961224
version: 1
top level: LacI
direction: direction/forward
start: 137
end: 142
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1961225
display id: annotation1961225
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
type: Range
persistent identity: range1961225
display id: range1961225
version: 1
top level: LacI
direction: direction/forward
start: 161
end: 166
orientation: inline

LacI binding site
type: SequenceAnnotation
persistent identity: annotation1961226
display id: annotation1961226
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
type: Range
persistent identity: range1961226
display id: range1961226
version: 1
top level: LacI
direction: direction/forward
start: 166
end: 200
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1961227
display id: annotation1961227
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
type: Range
persistent identity: range1961227
display id: range1961227
version: 1
top level: LacI
direction: direction/forward
start: 173
end: 173
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

BBa_B0030
type: ComponentDefinition
persistent identity: BBa B0030
display id: BBa_B0030
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.1 (strong) -- modified from R. Weiss
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _44_46_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: RBS-1\Strong
sequence annotation: BBa_B0030
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0030_sequence/1

RBS-1\Strong
type: SequenceAnnotation
persistent identity: annotation1701
display id: annotation1701
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
type: Range
persistent identity: range1701
display id: range1701
version: 1
top level: BBa_B0030
direction: direction/forward
start: 1
end: 15
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation7025
display id: annotation7025
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
type: Range
persistent identity: range7025
display id: range7025
version: 1
top level: BBa_B0030
direction: direction/forward
start: 1
end: 15
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1702
display id: annotation1702
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
type: Range
persistent identity: range1702
display id: range1702
version: 1
top level: BBa_B0030
direction: direction/forward
start: 8
end: 12
orientation: inline

Hin
type: ComponentDefinition
persistent identity: BBa J31000
display id: BBa_J31000
version: 1
was generated by: iGEM to SBOL conversion
description: DNA-invertase Hin from Salmonella typhimurium
created: 2006-06-04T11:00:00Z
modified: 2015-08-31T04:08:45Z
mutable provenance: Salmonella typhimurium
owned by: myers
owned by: james
mutable description: Generates the Salmonella typhimurium Hin protein. This protein is used to invert DNA that is flanked by Hix siteds.
discontinued: false
dominant: false
experience: experience/Works
group u list: _61_
m user id: 0
owner id: 918
owning group id: 61
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: none really
star: false
creator: Erin Zwack, Sabriya Rosemond
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: Fwd primer J31001
sequence annotation: Hin sequence
sequence annotation: Former SpeI site
sequence annotation: Mutation to delete the SpeI site
sequence annotation: Rev primer J31001
sequence: https://synbiohub.org/public/igem/BBa_J31000_sequence/1

Fwd primer J31001
type: SequenceAnnotation
persistent identity: annotation1910568
display id: annotation1910568
version: 1
top level: Hin
location: https://synbiohub.org/public/igem/BBa_J31000/annotation1910568/range1910568/1
role: feature/primer binding
role: SO:0005850

https://synbiohub.org/public/igem/BBa_J31000/annotation1910568/range1910568/1
type: Range
persistent identity: range1910568
display id: range1910568
version: 1
top level: Hin
direction: direction/forward
start: 1
end: 3
orientation: inline

Hin sequence
type: SequenceAnnotation
persistent identity: annotation1884987
display id: annotation1884987
version: 1
top level: Hin
location: https://synbiohub.org/public/igem/BBa_J31000/annotation1884987/range1884987/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_J31000/annotation1884987/range1884987/1
type: Range
persistent identity: range1884987
display id: range1884987
version: 1
top level: Hin
direction: direction/forward
start: 1
end: 573
orientation: inline

Former SpeI site
type: SequenceAnnotation
persistent identity: annotation1880459
display id: annotation1880459
version: 1
top level: Hin
location: https://synbiohub.org/public/igem/BBa_J31000/annotation1880459/range1880459/1
role: feature/mutation
role: SO:0001059

https://synbiohub.org/public/igem/BBa_J31000/annotation1880459/range1880459/1
type: Range
persistent identity: range1880459
display id: range1880459
version: 1
top level: Hin
direction: direction/forward
start: 70
end: 75
orientation: inline

Mutation to delete the SpeI site
type: SequenceAnnotation
persistent identity: annotation1880460
display id: annotation1880460
version: 1
top level: Hin
location: https://synbiohub.org/public/igem/BBa_J31000/annotation1880460/range1880460/1
role: feature/mutation
role: SO:0001059

https://synbiohub.org/public/igem/BBa_J31000/annotation1880460/range1880460/1
type: Range
persistent identity: range1880460
display id: range1880460
version: 1
top level: Hin
direction: direction/forward
start: 72
end: 72
orientation: inline

Rev primer J31001
type: SequenceAnnotation
persistent identity: annotation1910569
display id: annotation1910569
version: 1
top level: Hin
location: https://synbiohub.org/public/igem/BBa_J31000/annotation1910569/range1910569/1
role: feature/primer binding
role: SO:0005850

https://synbiohub.org/public/igem/BBa_J31000/annotation1910569/range1910569/1
type: Range
persistent identity: range1910569
display id: range1910569
version: 1
top level: Hin
direction: direction/reverse
start: 551
end: 570
orientation: inline

BBa_I13458
type: ComponentDefinition
persistent identity: BBa I13458
display id: BBa_I13458
version: 1
was generated by: iGEM to SBOL conversion
description: promoter C and arabinose C operon
created: 2005-08-16T11:00:00Z
modified: 2015-08-31T04:07:34Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: PC and AraC from the AraBAD operator. PC and AraC are on the complementary strand, reading right to left.
discontinued: false
dominant: false
group u list: _11_
m user id: 0
owner id: 253
owning group id: 6
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Josh Michener
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence: https://synbiohub.org/public/igem/BBa_I13458_sequence/1

https://synbiohub.org/public/igem/BBa_J3107_sequence/1
type: Sequence
persistent identity: BBa J3107 sequence
display id: BBa_J3107_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctattactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagcaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagattaaagaggagaaatactagatggctactattgggtatattcgggtgtcaacaattgaccaaaatatcgatttacagcgtaatgcgcttaccagtgcaaattgtgaccgcatttttgaagaccgtatcagtggcaagattgcaaaccgccccggcctgaaacgggcgttaaagtatgtaaataaaggcgatactcttgtcgtctggaaattagacagactgggccgtagcgtgaaaaatctggtggcgttaatatcagaattacatgaacgtggagctcacttccattctttaaccgatagtattgataccagtagcgcgatggggcgattcttttttcatgtaatgtcagcactggccgagatggagcgagaattaatcgtcgagcgaacccttgccggactggctgccgccagagcgcaaggacgactgggagggcgccctcgggcgatcaacaaacatgaacaggaacagattagtcggctattagagaaaggccatcctcggcagcaattagctattatttttggtat…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0010_sequence/1
type: Sequence
persistent identity: BBa R0010 sequence
display id: BBa_R0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0030_sequence/1
type: Sequence
persistent identity: BBa B0030 sequence
display id: BBa_B0030_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attaaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J31000_sequence/1
type: Sequence
persistent identity: BBa J31000 sequence
display id: BBa_J31000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggctactattgggtatattcgggtgtcaacaattgaccaaaatatcgatttacagcgtaatgcgcttaccagtgcaaattgtgaccgcatttttgaagaccgtatcagtggcaagattgcaaaccgccccggcctgaaacgggcgttaaagtatgtaaataaaggcgatactcttgtcgtctggaaattagacagactgggccgtagcgtgaaaaatctggtggcgttaatatcagaattacatgaacgtggagctcacttccattctttaaccgatagtattgataccagtagcgcgatggggcgattcttttttcatgtaatgtcagcactggccgagatggagcgagaattaatcgtcgagcgaacccttgccggactggctgccgccagagcgcaaggacgactgggagggcgccctcgggcgatcaacaaacatgaacaggaacagattagtcggctattagagaaaggccatcctcggcagcaattagctattatttttggtattggcgtatccaccttatacagatactttccggcaagcagtataaaaaaacgaatgaattaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I13458_sequence/1
type: Sequence
persistent identity: BBa I13458 sequence
display id: BBa_I13458_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctat
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z