LOCUS BBa_J32001 2942 bp DNA linear 22-Nov-2024 DEFINITION Targeted Localization ACCESSION BBa_J32001 VERSION BBa_J32001.1 FEATURES Location/Qualifiers misc_feature 1..19 /label=TetR 1 misc_feature 1..54 /label=BBa_R0040 promoter 20..25 /label=-35 misc_feature 26..44 /label=TetR 2 promoter 43..48 /label=-10 promoter 1..54 /label=BBa_R0040 misc_feature 67..70 /label=conserved RBS 63..74 /label=BBa_B0034 CDS 81..800 /label=GFP protein CDS 81..800 /label=BBa_E0040 stem_loop 820..863 /label=stem_loop misc_feature 809..888 /label=BBa_B0010 terminator 809..888 /label=BBa_B0010 stem_loop 904..923 /label=T7 TE misc_feature 930..930 /label=stop polyA_site 924..937 /label=polya misc_feature 897..937 /label=BBa_B0012 terminator 897..937 /label=BBa_B0012 misc_feature 946..964 /label=TetR 1 misc_feature 946..999 /label=BBa_R0040 promoter 965..970 /label=-35 misc_feature 971..989 /label=TetR 2 promoter 988..993 /label=-10 promoter 946..999 /label=BBa_R0040 misc_feature 1012..1015 /label=conserved RBS 1008..1019 /label=BBa_B0034 misc_feature 1026..1091 /label=PelB misc_feature 1026..1091 /label=BBa_J32015 CDS 1100..1486 /label=AntiCEA CDS 1100..1486 /label=BBa_J32016 misc_feature 1495..1540 /label=S-Tag misc_feature 1495..1540 /label=BBa_J32017 CDS 1549..2805 /label=C-IgAP CDS 1549..2805 /label=BBa_J32018 stem_loop 2825..2868 /label=stem_loop misc_feature 2814..2893 /label=BBa_B0010 terminator 2814..2893 /label=BBa_B0010 stem_loop 2909..2928 /label=T7 TE misc_feature 2935..2935 /label=stop polyA_site 2929..2942 /label=polya misc_feature 2902..2942 /label=BBa_B0012 terminator 2902..2942 /label=BBa_B0012 ORIGIN 1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag 61 agaaagagga gaaatactag atgcgtaaag gagaagaact tttcactgga gttgtcccaa 121 ttcttgttga attagatggt gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg 181 aaggtgatgc aacatacgga aaacttaccc ttaaatttat ttgcactact ggaaaactac 241 ctgttccatg gccaacactt gtcactactt tcggttatgg tgttcaatgc tttgcgagat 301 acccagatca tatgaaacag catgactttt tcaagagtgc catgcccgaa ggttatgtac 361 aggaaagaac tatatttttc aaagatgacg ggaactacaa gacacgtgct gaagtcaagt 421 ttgaaggtga tacccttgtt aatagaatcg agttaaaagg tattgatttt aaagaagatg 481 gaaacattct tggacacaaa ttggaataca actataactc acacaatgta tacatcatgg 541 cagacaaaca aaagaatgga atcaaagtta acttcaaaat tagacacaac attgaagatg 601 gaagcgttca actagcagac cattatcaac aaaatactcc aattggcgat ggccctgtcc 661 ttttaccaga caaccattac ctgtccacac aatctgccct ttcgaaagat cccaacgaaa 721 agagagacca catggtcctt cttgagtttg taacagctgc tgggattaca catggcatgg 781 atgaactata caaataataa tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg 841 aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac 901 actggctcac cttcgggtgg gcctttctgc gtttatatac tagagtccct atcagtgata 961 gagattgaca tccctatcag tgatagagat actgagcact actagagaaa gaggagaaat 1021 actagatgaa atacctgctg ccgaccgctg ctgctggtct gctgctcctc gctgcccagc 1081 cggcgatggc ctactagagc aggtgcagct ggtggagtct gggggaggct cggtgcaggc 1141 tggagggtct ctgagactct cctgtgcggc ctctggagac acctacggta gctactggat 1201 gggctggttc cgccaggctc cagggaagga gcgtgagggg gtcgcagcta ttaatagggg 1261 tggtggctat acagtctacg ccgactccgt gaagggccga ttcaccatct cccgagacac 1321 cgccaagaac acggtgtatc tgcaaatgaa cagcctgaga cctgacgaca cggccgacta 1381 ttactgtgcg gctagcgggg tactaggtgg tttacatgag gactggttta actactgggg 1441 ccaggggacc caggtcaccg tctcctcaca ccaccatcac catcactact agagaaaaga 1501 aaccgctgct gctaaattcg aacgccagca catggacagc tactagagaa agctgaggaa 1561 gaagagcacc gtcaaacagc ccaatcccag ccgcaacgcc gcaaacgccg tgccgcaccg 1621 caggattata tggcagtttc ccaagaccgt ccgaaacgtc gcggacgcag atctactctg 1681 ccggcaccgc cctcgccatc atttgattca tcagcttacg cagcacccag ggccttgcat 1741 aatccggact ggtatgaaaa tgattatgaa gaaatcccct tggatgcgct ggaagatgaa 1801 gatgtatccg aatcggttga cacatcagac aaacagcctc aagacaatac ggaacttcat 1861 gaaaaagttg aggcagtgag tttgcaacca agagccgcgc agccgcgaac ccaagccgcc 1921 gcgcaagccg atgcagtcag caccaatact aactcggctt tatctgacgc aatggcaagc 1981 acgcaatcta tcttgttgga tacaggtgct tcattaacac ggcacattgc acaaaaatca 2041 cgcgctgatg ccgaaaaaaa cagtgtttgg atgtcaaaca ccggttatgg ccgtgattat 2101 gcttccgcac aatatcgccg gtttagttcg aaacgcacgc aaacacaaat cggcattgac 2161 cgcagcttgt ccgaaaatat gcagataggc ggagtattga cttactctga cagtcagcat 2221 acttttgatc aggcgggcgg caaaaatact tttgtgcaag ccaaccttta tggtaagtat 2281 tatttaaatg atgcttggta tgtggccggc gatattggtg cgggcagctt gagaagccgg 2341 ttacaaacgc agcaaaaagc aaactttaac cgaacaagca tccaaaccgg ccttactttg 2401 ggcaatacgc tgaaaatcaa tcaattcgag attgtcccta gtgcgggtat ccgttacagc 2461 cgcctgtcat ctgcagatta caagttgggt gacgacagtg ttaaagtaag ttctatggca 2521 gtgaaaacac taacggccgg actggatttt gcttatcggt ttaaagtcgg caaccttacc 2581 gtaaaaccct tgttatctgc agcttacttt gccaattatg gcaaaggcgg cgtgaatgtg 2641 ggcggtaaat ccttcgccta taaagcagat aatcaacagc aatattcagc aggcgccgcg 2701 ttactgtacc gtaatgttac attaaacgta aatggcagta ttacaaaagg aaaacaattg 2761 gaaaaacaaa aatccggaca aattaaaata cagattcgtt tctaatacta gagccaggca 2821 tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc 2881 ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta 2941 ta //