BBa_E0040 1 GFP green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212 2004-09-29T11:00:00Z 2016-01-26T02:09:38Z Released HQ 2013 GFP (mut3b) [note that this part does not have a barcode] false true _11_1_ 4206 61 7 In stock false true jcbraff annotation1934520 1 GFP protein range1934520 1 1 720 BBa_J32016 1 AntiCEA AntiCEA 2006-08-24T11:00:00Z 2015-08-31T04:08:46Z Ablynx Carcenogenic Embryonic Antigen (CEA) antibody false false _50_ 0 495 50 Not in stock false . false Austen Heinz annotation1898183 1 AntiCEA range1898183 1 1 387 BBa_J32018 1 C-IgAP C-IgAP 2006-08-24T11:00:00Z 2015-08-31T04:08:46Z NCBI Accession AE004969. C Terminal Neisseria gonorrhoeae IgA Protease Allows for suface display of antibodies. false false _50_ 0 495 50 Not in stock false Removal of 2 PstI sites false Austen Heinz annotation1898185 1 C-IgAP range1898185 1 1 1257 BBa_J32001 1 BBa_J32001 Targeted Localization 2006-07-10T11:00:00Z 2015-08-31T04:08:45Z The signal sequence PelB and S*Tag sequence came from Novagen. The antibody sequence is for cAb-CEA5 nanobody from Ablynx. The C-terminus of Neisseria gonorrhoeae IgA Protease NCBI accession number AE004969 Neisseria gonorrhoeae IgA Protease antibody display. false false _50_ 0 495 50 Not in stock false It is a fusion protein so biobrick prefixes and suffixes have been modified to 10 and 24 bp, removing the bp between the flanking restriction sites and the sequence. Otherwise it would read out of frame with 8bp overlap false Austen Heinz component1902912 1 BBa_B0012 component1902931 1 BBa_B0010 component1902924 1 BBa_J32015 component1902917 1 BBa_R0040 component1902928 1 BBa_J32017 component1902922 1 BBa_B0034 component1902909 1 BBa_E0040 component1902926 1 BBa_J32016 component1902933 1 BBa_B0012 component1902930 1 BBa_J32018 component1902908 1 BBa_B0034 component1902903 1 BBa_R0040 component1902910 1 BBa_B0010 annotation1902917 1 BBa_R0040 range1902917 1 946 999 annotation1902910 1 BBa_B0010 range1902910 1 809 888 annotation1902930 1 BBa_J32018 range1902930 1 1549 2805 annotation1902922 1 BBa_B0034 range1902922 1 1008 1019 annotation1902928 1 BBa_J32017 range1902928 1 1495 1540 annotation1902931 1 BBa_B0010 range1902931 1 2814 2893 annotation1902903 1 BBa_R0040 range1902903 1 1 54 annotation1902912 1 BBa_B0012 range1902912 1 897 937 annotation1902933 1 BBa_B0012 range1902933 1 2902 2942 annotation1902926 1 BBa_J32016 range1902926 1 1100 1486 annotation1902924 1 BBa_J32015 range1902924 1 1026 1091 annotation1902908 1 BBa_B0034 range1902908 1 63 74 annotation1902909 1 BBa_E0040 range1902909 1 81 800 BBa_J32015 1 PelB PelB leader sequence; directs protein to E. coli periplasmic membrane 2006-08-24T11:00:00Z 2015-08-31T04:08:46Z Novagen Released HQ 2013 The pelB leader sequence is a sequence of amino acids which when attached to a protein, directs the protein to the periplasmic membrane of E. coli, where the sequence is removed by pelB peptidase. It is used to direct coat protein-antigen fusions to the cell surface. false true _50_ 0 495 50 In stock true . true Austen Heinz annotation1898182 1 PelB range1898182 1 1 66 BBa_J32017 1 S*Tag S-Tag 2006-08-24T11:00:00Z 2015-08-31T04:08:46Z Novagen The S???Tag sequence is a novel fusion peptide tag for recombinant proteins that allows detection by a rapid, sensitive homogeneous assay or by colorimetric detection in Western blots. Proteins can also be rapidly purified from crude extracts using a one step affinity separation method. The system is based on the strong interaction between the 15aa S???Tag and 103aa S-protein. false false _50_ 0 495 50 It's complicated true . false Austen Heinz annotation1898184 1 S-Tag range1898184 1 1 46 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation7018 1 BBa_B0010 range7018 1 1 80 annotation4184 1 stem_loop range4184 1 12 55 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1687 1 stop range1687 1 34 34 annotation1690 1 polya range1690 1 28 41 annotation1686 1 T7 TE range1686 1 8 27 annotation7020 1 BBa_B0012 range7020 1 1 41 BBa_R0040 1 p(tetR) TetR repressible promoter 2003-01-31T12:00:00Z 2015-05-08T01:14:14Z Lutz, R., Bujard, H., <em>Nucleic Acids Research</em> (1997) 25, 1203-1210. Released HQ 2013 Sequence for pTet inverting regulator driven by the TetR protein.</P> false true _1_ 0 24 7 In stock false <P> <P>BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.<P> true June Rhee, Connie Tao, Ty Thomson, Louis Waldman annotation1986784 1 BBa_R0040 range1986784 1 1 54 annotation1986785 1 -35 range1986785 1 20 25 annotation1986783 1 TetR 1 range1986783 1 1 19 annotation1986787 1 -10 range1986787 1 43 48 annotation1986786 1 TetR 2 range1986786 1 26 44 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_J32018_sequence 1 aaagctgaggaagaagagcaccgtcaaacagcccaatcccagccgcaacgccgcaaacgccgtgccgcaccgcaggattatatggcagtttcccaagaccgtccgaaacgtcgcggacgcagatctactctgccggcaccgccctcgccatcatttgattcatcagcttacgcagcacccagggccttgcataatccggactggtatgaaaatgattatgaagaaatccccttggatgcgctggaagatgaagatgtatccgaatcggttgacacatcagacaaacagcctcaagacaatacggaacttcatgaaaaagttgaggcagtgagtttgcaaccaagagccgcgcagccgcgaacccaagccgccgcgcaagccgatgcagtcagcaccaatactaactcggctttatctgacgcaatggcaagcacgcaatctatcttgttggatacaggtgcttcattaacacggcacattgcacaaaaatcacgcgctgatgccgaaaaaaacagtgtttggatgtcaaacaccggttatggccgtgattatgcttccgcacaatatcgccggtttagttcgaaacgcacgcaaacacaaatcggcattgaccgcagcttgtccgaaaatatgcagataggcggagtattgacttactctgacagtcagcatacttttgatcaggcgggcggcaaaaatacttttgtgcaagccaacctttatggtaagtattatttaaatgatgcttggtatgtggccggcgatattggtgcgggcagcttgagaagccggttacaaacgcagcaaaaagcaaactttaaccgaacaagcatccaaaccggccttactttgggcaatacgctgaaaatcaatcaattcgagattgtccctagtgcgggtatccgttacagccgcctgtcatctgcagattacaagttgggtgacgacagtgttaaagtaagttctatggcagtgaaaacactaacggccggactggattttgcttatcggtttaaagtcggcaaccttaccgtaaaacccttgttatctgcagcttactttgccaattatggcaaaggcggcgtgaatgtgggcggtaaatccttcgcctataaagcagataatcaacagcaatattcagcaggcgccgcgttactgtaccgtaatgttacattaaacgtaaatggcagtattacaaaaggaaaacaattggaaaaacaaaaatccggacaaattaaaatacagattcgtttctaa BBa_B0034_sequence 1 aaagaggagaaa BBa_R0040_sequence 1 tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac BBa_J32016_sequence 1 caggtgcagctggtggagtctgggggaggctcggtgcaggctggagggtctctgagactctcctgtgcggcctctggagacacctacggtagctactggatgggctggttccgccaggctccagggaaggagcgtgagggggtcgcagctattaataggggtggtggctatacagtctacgccgactccgtgaagggccgattcaccatctcccgagacaccgccaagaacacggtgtatctgcaaatgaacagcctgagacctgacgacacggccgactattactgtgcggctagcggggtactaggtggtttacatgaggactggtttaactactggggccaggggacccaggtcaccgtctcctcacaccaccatcaccatcac BBa_J32001_sequence 1 tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatgaaatacctgctgccgaccgctgctgctggtctgctgctcctcgctgcccagccggcgatggcctactagagcaggtgcagctggtggagtctgggggaggctcggtgcaggctggagggtctctgagactctcctgtgcggcctctggagacacctacggtagctactggatgggctggttccgccaggctccagggaaggagcgtgagggggtcgcagctattaataggggtggtggctatacagtctacgccgactccgtgaagggccgattcaccatctcccgagacaccgccaagaacacggtgtatctgcaaatgaacagcctgagacctgacgacacggccgactattactgtgcggctagcggggtactaggtggtttacatgaggactggtttaactactggggccaggggacccaggtcaccgtctcctcacaccaccatcaccatcactactagagaaaagaaaccgctgctgctaaattcgaacgccagcacatggacagctactagagaaagctgaggaagaagagcaccgtcaaacagcccaatcccagccgcaacgccgcaaacgccgtgccgcaccgcaggattatatggcagtttcccaagaccgtccgaaacgtcgcggacgcagatctactctgccggcaccgccctcgccatcatttgattcatcagcttacgcagcacccagggccttgcataatccggactggtatgaaaatgattatgaagaaatccccttggatgcgctggaagatgaagatgtatccgaatcggttgacacatcagacaaacagcctcaagacaatacggaacttcatgaaaaagttgaggcagtgagtttgcaaccaagagccgcgcagccgcgaacccaagccgccgcgcaagccgatgcagtcagcaccaatactaactcggctttatctgacgcaatggcaagcacgcaatctatcttgttggatacaggtgcttcattaacacggcacattgcacaaaaatcacgcgctgatgccgaaaaaaacagtgtttggatgtcaaacaccggttatggccgtgattatgcttccgcacaatatcgccggtttagttcgaaacgcacgcaaacacaaatcggcattgaccgcagcttgtccgaaaatatgcagataggcggagtattgacttactctgacagtcagcatacttttgatcaggcgggcggcaaaaatacttttgtgcaagccaacctttatggtaagtattatttaaatgatgcttggtatgtggccggcgatattggtgcgggcagcttgagaagccggttacaaacgcagcaaaaagcaaactttaaccgaacaagcatccaaaccggccttactttgggcaatacgctgaaaatcaatcaattcgagattgtccctagtgcgggtatccgttacagccgcctgtcatctgcagattacaagttgggtgacgacagtgttaaagtaagttctatggcagtgaaaacactaacggccggactggattttgcttatcggtttaaagtcggcaaccttaccgtaaaacccttgttatctgcagcttactttgccaattatggcaaaggcggcgtgaatgtgggcggtaaatccttcgcctataaagcagataatcaacagcaatattcagcaggcgccgcgttactgtaccgtaatgttacattaaacgtaaatggcagtattacaaaaggaaaacaattggaaaaacaaaaatccggacaaattaaaatacagattcgtttctaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_J32015_sequence 1 atgaaatacctgctgccgaccgctgctgctggtctgctgctcctcgctgcccagccggcgatggcc BBa_E0040_sequence 1 atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa BBa_J32017_sequence 1 aaaagaaaccgctgctgctaaattcgaacgccagcacatggacagc BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z