BBa_J32001
type: ComponentDefinition
persistent identity: BBa J32001
display id: BBa_J32001
version: 1
was generated by: iGEM to SBOL conversion
description: Targeted Localization
created: 2006-07-10T11:00:00Z
modified: 2015-08-31T04:08:45Z
mutable provenance: The signal sequence PelB and S*Tag sequence came from Novagen. The antibody sequence is for cAb-CEA5 nanobody from Ablynx. The C-terminus of Neisseria gonorrhoeae IgA Protease NCBI accession number AE004969
owned by: james
owned by: myers
mutable description: Neisseria gonorrhoeae IgA Protease antibody display.
discontinued: false
dominant: false
experience: experience/None
group u list: _50_
m user id: 0
owner id: 495
owning group id: 50
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: It is a fusion protein so biobrick prefixes and suffixes have been modified to 10 and 24 bp, removing the bp between the flanking restriction sites and the sequence. Otherwise it would read out of frame with 8bp overlap
star: false
creator: Austen Heinz
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_R0040
component: BBa_E0040
component: BBa_B0034
component: BBa_B0012
component: BBa_B0010
component: BBa_J32016
component: BBa_J32015
component: BBa_J32018
component: BBa_J32017
sequence annotation: BBa_R0040
sequence annotation: BBa_B0034
sequence annotation: BBa_E0040
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_J32015
sequence annotation: BBa_J32016
sequence annotation: BBa_J32017
sequence annotation: BBa_J32018
sequence: https://synbiohub.org/public/igem/BBa_J32001_sequence/1

BBa_R0040
type: Component
persistent identity: component1902903
display id: component1902903
version: 1
top level: BBa_J32001
definition: p(tetR)
access: public

BBa_E0040
type: Component
persistent identity: component1902909
display id: component1902909
version: 1
top level: BBa_J32001
definition: GFP
access: public

BBa_B0034
type: Component
persistent identity: component1902908
display id: component1902908
version: 1
top level: BBa_J32001
definition: BBa_B0034
access: public

BBa_B0012
type: Component
persistent identity: component1902912
display id: component1902912
version: 1
top level: BBa_J32001
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1902910
display id: component1902910
version: 1
top level: BBa_J32001
definition: BBa_B0010
access: public

BBa_B0034
type: Component
persistent identity: component1902922
display id: component1902922
version: 1
top level: BBa_J32001
definition: BBa_B0034
access: public

BBa_R0040
type: Component
persistent identity: component1902917
display id: component1902917
version: 1
top level: BBa_J32001
definition: p(tetR)
access: public

BBa_J32016
type: Component
persistent identity: component1902926
display id: component1902926
version: 1
top level: BBa_J32001
definition: AntiCEA
access: public

BBa_J32015
type: Component
persistent identity: component1902924
display id: component1902924
version: 1
top level: BBa_J32001
definition: PelB
access: public

BBa_J32018
type: Component
persistent identity: component1902930
display id: component1902930
version: 1
top level: BBa_J32001
definition: C-IgAP
access: public

BBa_J32017
type: Component
persistent identity: component1902928
display id: component1902928
version: 1
top level: BBa_J32001
definition: S*Tag
access: public

BBa_B0012
type: Component
persistent identity: component1902933
display id: component1902933
version: 1
top level: BBa_J32001
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1902931
display id: component1902931
version: 1
top level: BBa_J32001
definition: BBa_B0010
access: public

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1902903
display id: annotation1902903
version: 1
top level: BBa_J32001
location: https://synbiohub.org/public/igem/BBa_J32001/annotation1902903/range1902903/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_J32001/annotation1902903/range1902903/1
type: Range
persistent identity: range1902903
display id: range1902903
version: 1
top level: BBa_J32001
direction: direction/forward
start: 1
end: 54
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1902908
display id: annotation1902908
version: 1
top level: BBa_J32001
location: https://synbiohub.org/public/igem/BBa_J32001/annotation1902908/range1902908/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J32001/annotation1902908/range1902908/1
type: Range
persistent identity: range1902908
display id: range1902908
version: 1
top level: BBa_J32001
direction: direction/reverse
start: 63
end: 74
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1902909
display id: annotation1902909
version: 1
top level: BBa_J32001
location: https://synbiohub.org/public/igem/BBa_J32001/annotation1902909/range1902909/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_J32001/annotation1902909/range1902909/1
type: Range
persistent identity: range1902909
display id: range1902909
version: 1
top level: BBa_J32001
direction: direction/reverse
start: 81
end: 800
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1902910
display id: annotation1902910
version: 1
top level: BBa_J32001
location: https://synbiohub.org/public/igem/BBa_J32001/annotation1902910/range1902910/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J32001/annotation1902910/range1902910/1
type: Range
persistent identity: range1902910
display id: range1902910
version: 1
top level: BBa_J32001
direction: direction/forward
start: 809
end: 888
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1902912
display id: annotation1902912
version: 1
top level: BBa_J32001
location: https://synbiohub.org/public/igem/BBa_J32001/annotation1902912/range1902912/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J32001/annotation1902912/range1902912/1
type: Range
persistent identity: range1902912
display id: range1902912
version: 1
top level: BBa_J32001
direction: direction/forward
start: 897
end: 937
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1902917
display id: annotation1902917
version: 1
top level: BBa_J32001
location: https://synbiohub.org/public/igem/BBa_J32001/annotation1902917/range1902917/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_J32001/annotation1902917/range1902917/1
type: Range
persistent identity: range1902917
display id: range1902917
version: 1
top level: BBa_J32001
direction: direction/forward
start: 946
end: 999
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1902922
display id: annotation1902922
version: 1
top level: BBa_J32001
location: https://synbiohub.org/public/igem/BBa_J32001/annotation1902922/range1902922/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J32001/annotation1902922/range1902922/1
type: Range
persistent identity: range1902922
display id: range1902922
version: 1
top level: BBa_J32001
direction: direction/reverse
start: 1008
end: 1019
orientation: inline

BBa_J32015
type: SequenceAnnotation
persistent identity: annotation1902924
display id: annotation1902924
version: 1
top level: BBa_J32001
location: https://synbiohub.org/public/igem/BBa_J32001/annotation1902924/range1902924/1
component: BBa_J32015

https://synbiohub.org/public/igem/BBa_J32001/annotation1902924/range1902924/1
type: Range
persistent identity: range1902924
display id: range1902924
version: 1
top level: BBa_J32001
direction: direction/reverse
start: 1026
end: 1091
orientation: inline

BBa_J32016
type: SequenceAnnotation
persistent identity: annotation1902926
display id: annotation1902926
version: 1
top level: BBa_J32001
location: https://synbiohub.org/public/igem/BBa_J32001/annotation1902926/range1902926/1
component: BBa_J32016

https://synbiohub.org/public/igem/BBa_J32001/annotation1902926/range1902926/1
type: Range
persistent identity: range1902926
display id: range1902926
version: 1
top level: BBa_J32001
direction: direction/reverse
start: 1100
end: 1486
orientation: inline

BBa_J32017
type: SequenceAnnotation
persistent identity: annotation1902928
display id: annotation1902928
version: 1
top level: BBa_J32001
location: https://synbiohub.org/public/igem/BBa_J32001/annotation1902928/range1902928/1
component: BBa_J32017

https://synbiohub.org/public/igem/BBa_J32001/annotation1902928/range1902928/1
type: Range
persistent identity: range1902928
display id: range1902928
version: 1
top level: BBa_J32001
direction: direction/reverse
start: 1495
end: 1540
orientation: inline

BBa_J32018
type: SequenceAnnotation
persistent identity: annotation1902930
display id: annotation1902930
version: 1
top level: BBa_J32001
location: https://synbiohub.org/public/igem/BBa_J32001/annotation1902930/range1902930/1
component: BBa_J32018

https://synbiohub.org/public/igem/BBa_J32001/annotation1902930/range1902930/1
type: Range
persistent identity: range1902930
display id: range1902930
version: 1
top level: BBa_J32001
direction: direction/reverse
start: 1549
end: 2805
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1902931
display id: annotation1902931
version: 1
top level: BBa_J32001
location: https://synbiohub.org/public/igem/BBa_J32001/annotation1902931/range1902931/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J32001/annotation1902931/range1902931/1
type: Range
persistent identity: range1902931
display id: range1902931
version: 1
top level: BBa_J32001
direction: direction/forward
start: 2814
end: 2893
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1902933
display id: annotation1902933
version: 1
top level: BBa_J32001
location: https://synbiohub.org/public/igem/BBa_J32001/annotation1902933/range1902933/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J32001/annotation1902933/range1902933/1
type: Range
persistent identity: range1902933
display id: range1902933
version: 1
top level: BBa_J32001
direction: direction/forward
start: 2902
end: 2942
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

p(tetR)
type: ComponentDefinition
persistent identity: BBa R0040
display id: BBa_R0040
version: 1
was generated by: iGEM to SBOL conversion
description: TetR repressible promoter
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: Lutz, R., Bujard, H., Nucleic Acids Research (1997) 25, 1203-1210.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Sequence for pTet inverting regulator driven by the TetR protein.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0040
sequence annotation: TetR 1
sequence annotation: -35
sequence annotation: TetR 2
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0040_sequence/1

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1986784
display id: annotation1986784
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
type: Range
persistent identity: range1986784
display id: range1986784
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 54
orientation: inline

TetR 1
type: SequenceAnnotation
persistent identity: annotation1986783
display id: annotation1986783
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
type: Range
persistent identity: range1986783
display id: range1986783
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 19
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1986785
display id: annotation1986785
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
type: Range
persistent identity: range1986785
display id: range1986785
version: 1
top level: p(tetR)
direction: direction/forward
start: 20
end: 25
orientation: inline

TetR 2
type: SequenceAnnotation
persistent identity: annotation1986786
display id: annotation1986786
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
type: Range
persistent identity: range1986786
display id: range1986786
version: 1
top level: p(tetR)
direction: direction/forward
start: 26
end: 44
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1986787
display id: annotation1986787
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
type: Range
persistent identity: range1986787
display id: range1986787
version: 1
top level: p(tetR)
direction: direction/forward
start: 43
end: 48
orientation: inline

GFP
type: ComponentDefinition
persistent identity: BBa E0040
display id: BBa_E0040
version: 1
was generated by: iGEM to SBOL conversion
description: green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212
created: 2004-09-29T11:00:00Z
modified: 2016-01-26T02:09:38Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: GFP (mut3b) [note that this part does not have a barcode]
discontinued: false
dominant: true
experience: experience/Works
group u list: _11_1_
m user id: 4206
owner id: 61
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: jcbraff
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: GFP protein
sequence: https://synbiohub.org/public/igem/BBa_E0040_sequence/1

GFP protein
type: SequenceAnnotation
persistent identity: annotation1934520
display id: annotation1934520
version: 1
top level: GFP
location: https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
type: Range
persistent identity: range1934520
display id: range1934520
version: 1
top level: GFP
direction: direction/forward
start: 1
end: 720
orientation: inline

PelB
type: ComponentDefinition
persistent identity: BBa J32015
display id: BBa_J32015
version: 1
was generated by: iGEM to SBOL conversion
description: PelB leader sequence; directs protein to E. coli periplasmic membrane
created: 2006-08-24T11:00:00Z
modified: 2015-08-31T04:08:46Z
mutable provenance: Novagen
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: The pelB leader sequence is a sequence of amino acids which when attached to a protein, directs the protein to the periplasmic membrane of E. coli, where the sequence is removed by pelB peptidase. It is used to direct coat protein-antigen fusions to the cell surface.
discontinued: false
dominant: true
experience: experience/None
group u list: _50_
m user id: 0
owner id: 495
owning group id: 50
sample status: In stock
status: status/Available
bookmark: true
mutable notes: .
star: true
creator: Austen Heinz
type: DnaRegion
role: partType/Tag
role: SO:0000324
sequence annotation: PelB
sequence: https://synbiohub.org/public/igem/BBa_J32015_sequence/1

PelB
type: SequenceAnnotation
persistent identity: annotation1898182
display id: annotation1898182
version: 1
top level: PelB
location: https://synbiohub.org/public/igem/BBa_J32015/annotation1898182/range1898182/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_J32015/annotation1898182/range1898182/1
type: Range
persistent identity: range1898182
display id: range1898182
version: 1
top level: PelB
direction: direction/forward
start: 1
end: 66
orientation: inline

AntiCEA
type: ComponentDefinition
persistent identity: BBa J32016
display id: BBa_J32016
version: 1
was generated by: iGEM to SBOL conversion
description: AntiCEA
created: 2006-08-24T11:00:00Z
modified: 2015-08-31T04:08:46Z
mutable provenance: Ablynx
owned by: james
owned by: myers
mutable description: Carcenogenic Embryonic Antigen (CEA) antibody
discontinued: false
dominant: false
experience: experience/None
group u list: _50_
m user id: 0
owner id: 495
owning group id: 50
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: .
star: false
creator: Austen Heinz
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: AntiCEA
sequence: https://synbiohub.org/public/igem/BBa_J32016_sequence/1

AntiCEA
type: SequenceAnnotation
persistent identity: annotation1898183
display id: annotation1898183
version: 1
top level: AntiCEA
location: https://synbiohub.org/public/igem/BBa_J32016/annotation1898183/range1898183/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_J32016/annotation1898183/range1898183/1
type: Range
persistent identity: range1898183
display id: range1898183
version: 1
top level: AntiCEA
direction: direction/forward
start: 1
end: 387
orientation: inline

S*Tag
type: ComponentDefinition
persistent identity: BBa J32017
display id: BBa_J32017
version: 1
was generated by: iGEM to SBOL conversion
description: S-Tag
created: 2006-08-24T11:00:00Z
modified: 2015-08-31T04:08:46Z
mutable provenance: Novagen
owned by: james
owned by: myers
mutable description: The S???Tag sequence is a novel fusion peptide tag for recombinant proteins that allows detection by a rapid, sensitive homogeneous assay or by colorimetric detection in Western blots. Proteins can also be rapidly purified from crude extracts using a one step affinity separation method. The system is based on the strong interaction between the 15aa S???Tag and 103aa S-protein.
discontinued: false
dominant: false
experience: experience/None
group u list: _50_
m user id: 0
owner id: 495
owning group id: 50
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: .
star: false
creator: Austen Heinz
type: DnaRegion
role: SO:0000324
role: partType/Tag
sequence annotation: S-Tag
sequence: https://synbiohub.org/public/igem/BBa_J32017_sequence/1

S-Tag
type: SequenceAnnotation
persistent identity: annotation1898184
display id: annotation1898184
version: 1
top level: S*Tag
location: https://synbiohub.org/public/igem/BBa_J32017/annotation1898184/range1898184/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_J32017/annotation1898184/range1898184/1
type: Range
persistent identity: range1898184
display id: range1898184
version: 1
top level: S*Tag
direction: direction/forward
start: 1
end: 46
orientation: inline

C-IgAP
type: ComponentDefinition
persistent identity: BBa J32018
display id: BBa_J32018
version: 1
was generated by: iGEM to SBOL conversion
description: C-IgAP
created: 2006-08-24T11:00:00Z
modified: 2015-08-31T04:08:46Z
mutable provenance: NCBI Accession AE004969.
owned by: james
owned by: myers
mutable description: C Terminal Neisseria gonorrhoeae IgA Protease Allows for suface display of antibodies.
discontinued: false
dominant: false
experience: experience/None
group u list: _50_
m user id: 0
owner id: 495
owning group id: 50
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Removal of 2 PstI sites
star: false
creator: Austen Heinz
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: C-IgAP
sequence: https://synbiohub.org/public/igem/BBa_J32018_sequence/1

C-IgAP
type: SequenceAnnotation
persistent identity: annotation1898185
display id: annotation1898185
version: 1
top level: C-IgAP
location: https://synbiohub.org/public/igem/BBa_J32018/annotation1898185/range1898185/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_J32018/annotation1898185/range1898185/1
type: Range
persistent identity: range1898185
display id: range1898185
version: 1
top level: C-IgAP
direction: direction/forward
start: 1
end: 1257
orientation: inline

https://synbiohub.org/public/igem/BBa_J32001_sequence/1
type: Sequence
persistent identity: BBa J32001 sequence
display id: BBa_J32001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatgaaatacctgctgccgaccgctgctgctggtctgctgctcctcgctgcccagccggcgatggcctactagagcaggtgcagctggtggagtctgggggaggctcggtgcaggctggagggtctctgagactctcctgtgcggcctctggagacacctacggtagctactggatgggctggttccgccaggctccagggaaggagcgtgagggggtcgcagctattaataggggtggtggctatacagtctacgccgactccgtgaagggccgattcaccatctcccgagacaccgccaagaacacggtgtatctgcaaatgaacagcctgagacctgacgacacggccgactattactgtgcggctagcggggtactaggtggtttacatgaggactggtttaactactggggccaggggacccaggtcaccgtctcctcacaccaccatcaccatcactactagagaaaagaaaccgctgctgctaaattcgaacgccagcacatggacagctactagagaaagctgaggaagaagagcaccgtcaaacagcccaatcccagccgcaacgccgcaaacgccgtgccgcaccgcaggattatatggcagtttcccaagaccgtccgaaacgtcgcggacgcagatctactctgccggcaccgccctcgccatcatttgattcatcagcttacgcagcacccagggccttgcataatccggactggtatgaaaatgattatgaagaaatccccttggatgcgctggaagatgaagatgtatccgaatcggttgacacatcagacaaacagcctcaagacaatacggaacttcatgaaaaagttgaggcagtgagtttgcaaccaagagccgcgcagccgcgaacccaagccgccgcgcaagccgatgcagtcagcaccaatactaactcggctttatctgacgcaatggcaagcacgcaatctatcttgttgga…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J32016_sequence/1
type: Sequence
persistent identity: BBa J32016 sequence
display id: BBa_J32016_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caggtgcagctggtggagtctgggggaggctcggtgcaggctggagggtctctgagactctcctgtgcggcctctggagacacctacggtagctactggatgggctggttccgccaggctccagggaaggagcgtgagggggtcgcagctattaataggggtggtggctatacagtctacgccgactccgtgaagggccgattcaccatctcccgagacaccgccaagaacacggtgtatctgcaaatgaacagcctgagacctgacgacacggccgactattactgtgcggctagcggggtactaggtggtttacatgaggactggtttaactactggggccaggggacccaggtcaccgtctcctcacaccaccatcaccatcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0040_sequence/1
type: Sequence
persistent identity: BBa R0040 sequence
display id: BBa_R0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0040_sequence/1
type: Sequence
persistent identity: BBa E0040 sequence
display id: BBa_E0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: myers
owned by: james
elements: atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J32015_sequence/1
type: Sequence
persistent identity: BBa J32015 sequence
display id: BBa_J32015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaaatacctgctgccgaccgctgctgctggtctgctgctcctcgctgcccagccggcgatggcc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J32017_sequence/1
type: Sequence
persistent identity: BBa J32017 sequence
display id: BBa_J32017_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaaagaaaccgctgctgctaaattcgaacgccagcacatggacagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J32018_sequence/1
type: Sequence
persistent identity: BBa J32018 sequence
display id: BBa_J32018_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagctgaggaagaagagcaccgtcaaacagcccaatcccagccgcaacgccgcaaacgccgtgccgcaccgcaggattatatggcagtttcccaagaccgtccgaaacgtcgcggacgcagatctactctgccggcaccgccctcgccatcatttgattcatcagcttacgcagcacccagggccttgcataatccggactggtatgaaaatgattatgaagaaatccccttggatgcgctggaagatgaagatgtatccgaatcggttgacacatcagacaaacagcctcaagacaatacggaacttcatgaaaaagttgaggcagtgagtttgcaaccaagagccgcgcagccgcgaacccaagccgccgcgcaagccgatgcagtcagcaccaatactaactcggctttatctgacgcaatggcaagcacgcaatctatcttgttggatacaggtgcttcattaacacggcacattgcacaaaaatcacgcgctgatgccgaaaaaaacagtgtttggatgtcaaacaccggttatggccgtgattatgcttccgcacaatatcgccggtttagttcgaaacgcacgcaaacacaaatcggcattgaccgcagcttgtccgaaaatatgcagataggcggagtattgacttactctgacagtcagcatacttttgatcaggcgggcggcaaaaatacttttgtgcaagccaacctttatggtaagtattatttaaatgatgcttggtatgtggccggcgatattggtgcgggcagcttgagaagccggttacaaacgcagcaaaaagcaaactttaaccgaacaagcatccaaaccggccttactttgggcaatacgctgaaaatcaatcaattcgagattgtccctagtgcgggtatccgttacagccgcctgtcatctgcagattacaagttgggtgacgacagtgttaaagtaagttctatggcagtgaaaacactaacggccggactggattttgcttatcggtttaaagtcggcaaccttaccgtaaaacccttgttatctgcagcttactttgccaattatggcaaaggcggcgtgaatgtgggcggtaaatccttcgcctataaagcagataatcaacagcaatattcagcaggcgccgcgttactgtaccgtaatgttacattaaacgtaaatggcagtattacaaaaggaaaacaattggaaaaacaaaaatccggacaaattaaaatacagattcgtttctaa
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z