##gff-version 3
##sequence-region BBa_J32003 1 6371
BBa_J32003	.	terminator	6243	6371	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_J32003	.	terminator	6243	6322	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_J32003	.	engineered_region	6243	6322	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J32003	.	stem_loop	6254	6297	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J32003	.	terminator	6331	6371	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_J32003	.	stop_codon	6364	6364	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J32003	.	polyA_site	6358	6371	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J32003	.	stem_loop	6338	6357	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J32003	.	engineered_region	6331	6371	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J32003	.	CDS	1185	4175	.	+	0	ID=BBa_J32000;Name=BBa_J32000
BBa_J32003	.	engineered_region	1185	4175	.	+	0	ID=annotation1884317;Name=BBa_J3200;Parent=BBa_J32000
BBa_J32003	.	CDS	1185	4175	.	+	0	ID=annotation1884734;Name=invasin;Parent=BBa_J32000
BBa_J32003	.	ribosome_entry_site	4184	4195	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_J32003	.	conserved_region	4188	4191	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J32003	.	engineered_region	1	1158	.	+	0	ID=BBa_F2621;Name=BBa_F2621
BBa_J32003	.	terminator	967	1046	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_F2621
BBa_J32003	.	engineered_region	967	1046	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J32003	.	stem_loop	978	1021	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J32003	.	ribosome_entry_site	160	171	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_F2621
BBa_J32003	.	conserved_region	164	167	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J32003	.	promoter	1	151	.	+	0	ID=BBa_R0063;Name=BBa_R0063;Parent=BBa_F2621
BBa_J32003	.	promoter	115	122	.	+	0	ID=annotation2052;Name=-10;Parent=BBa_R0063
BBa_J32003	.	non_covalent_binding_site	130	149	.	+	0	ID=annotation2055;Name=Putative LuxR/HSL;Parent=BBa_R0063
BBa_J32003	.	non_covalent_binding_site	1	20	.	+	0	ID=annotation2051;Name=LuxR/HSL;Parent=BBa_R0063
BBa_J32003	.	engineered_region	1	151	.	+	0	ID=annotation7071;Name=BBa_R0063;Parent=BBa_R0063
BBa_J32003	.	start_codon	128	128	.	+	0	ID=annotation2054;Name=start;Parent=BBa_R0063
BBa_J32003	.	promoter	89	94	.	+	0	ID=annotation2053;Name=-35;Parent=BBa_R0063
BBa_J32003	.	promoter	1104	1158	.	+	0	ID=BBa_R0062;Name=BBa_R0062;Parent=BBa_F2621
BBa_J32003	.	start_codon	1156	1156	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_J32003	.	promoter	1145	1150	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_J32003	.	promoter	1123	1128	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_J32003	.	engineered_region	1104	1158	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_J32003	.	non_covalent_binding_site	1104	1123	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_J32003	.	CDS	178	933	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_F2621
BBa_J32003	.	engineered_tag	934	958	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_J32003	.	engineered_region	178	933	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_J32003	.	engineered_region	178	179	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_J32003	.	point_mutation	351	351	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_J32003	.	CDS	178	927	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_J32003	.	point_mutation	669	669	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_J32003	.	terminator	1055	1095	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_F2621
BBa_J32003	.	stop_codon	1088	1088	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J32003	.	polyA_site	1082	1095	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J32003	.	stem_loop	1062	1081	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J32003	.	engineered_region	1055	1095	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J32003	.	CDS	4202	5485	.	+	0	ID=BBa_J32002;Name=BBa_J32002
BBa_J32003	.	engineered_region	4202	5485	.	+	0	ID=annotation1884318;Name=J32002;Parent=BBa_J32002
BBa_J32003	.	CDS	4202	5485	.	+	0	ID=annotation1885001;Name=Cytosine Deaminase;Parent=BBa_J32002
BBa_J32003	.	ribosome_entry_site	5494	5505	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_J32003	.	conserved_region	5498	5501	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J32003	.	ribosome_entry_site	1167	1178	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_J32003	.	conserved_region	1171	1174	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J32003	.	CDS	5512	6234	.	+	0	ID=BBa_E0020;Name=BBa_E0020
>BBa_J32003
acctgtacgatcctacaggtgcttatgttaagtaattgtattcccagcgatacaatagtgtgacaaaaatccaatttat
tagaatcaaatgtcaatccattaccgttttaatgatatataacacgcaaaacttgcgacaaacaataggtaatactaga
gaaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtaga
agcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcattt
atcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgc
taatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaac
aatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttcccta
ttcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttattttt
acatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaa
tcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaa
aaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctg
ccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctag
tgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttg
tttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacc
tgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaatactagagaaagaggagaaatactaga
tgacaggtatgctttatttcatttaaattatgatggttttccagccaatcagtgagtttctcttgataaggaatgcggg
aatgtctatgtattttaataaaataatttcatttaatattatttcacgaatagttatttgtatctttttgatatgtgga
atgttcatggctggggcttcagaaaaatatgatgctaacgcaccgcaacaggtccagccttattctgtctcttcatctg
catttgaaaatctccatcctaataatgaaatggagagttcaatcaatcccttttccgcatcggatacagaaagaaatgc
tgcaataatagatcgcgccaataaggagcaggagactgaagcggtgaataagatgataagcaccggggccaggttagct
gcatcaggcagggcatctgatgttgctcactcaatggtgggcgatgcggttaatcaagaaatcaaacagtggttaaatc
gattcggtacggctcaagttaatctgaattttgacaaaaatttttcgctaaaagaaagctctcttgattggctggctcc
ttggtatgactctgcttcattcctcttttttagtcagttaggtattcgcaataaagacagccgcaacacacttaacctt
ggcgtcgggatacgtacattggagaacggttggctgtacggacttaatactttttatgataatgatttgaccggccaca
accaccgtatcggtcttggtgccgaggcctggaccgattatttacagttggctgccaatgggtattttcgcctcaatgg
atggcactcgtcgcgtgatttctccgactataaagagcgcccagccactgggggggatttgcgcgcgaatgcttattta
cctgcactcccacaactgggggggaagttgatgtatgagcaatacaccggtgagcgtgttgctttatttggtaaagata
atctgcaacgcaacccttatgccgtgactgccgggatcaattacacccccgtgcctctactcactgtcggggtagatca
gcgtatggggaaaagcagtaagcatgaaacacagtggaacctccaaatgaactatcgcctgggcgagagttttcagtcg
caacttagcccttcagcggtggcaggaacacgtctactggcggagagccgctataaccttgtcgatcgtaacaataata
tcgtgttggagtatcagaaacagcaggtggttaaactgacattatcgccagcaactatctccggcctgccgggtcaggt
ttatcaggtgaacgcacaagtacaaggggcatctgctgtaagggaaattgtctggagtgatgccgaactgattgccgct
ggcggcacattaacaccactgagtaccacacaattcaacttggttttaccgccttataaacgcacagcacaagtgagtc
gggtaacggacgacctgacagccaacttttattcgcttagtgcgctcgcggttgatcaccaaggaaaccgatctaactc
attcacattgagcgtcaccgttcagcagcctcagttgacattaacggcggccgtcattggtgatggcgcaccggctaat
gggaaaactgcaatcaccgttgagttcaccgttgctgattttgaggggaaacccttagccgggcaggaggtggtgataa
ccaccaataatggtgcgctaccgaataaaatcacggaaaagacagatgcaaatggcgtcgcgcgcattgcattaaccaa
tacgacagatggcgtgacggtagtcacagcagaagtggaggggcaacggcaaagtgttgatacccactttgttaagggt
actatcgcggcggataaatccactctggctgcggtaccgacatctatcatcgctgatggtctaatggcttcaaccatca
cgttggagttgaaggatacctatggggacccgcaggctggcgcgaatgtggcttttgacacaaccttaggcaatatggg
cgttatcacggatcacaatgacggcacttatagcgcaccattgaccagtaccacgttgggggtagcaacagtaacggtg
aaagtggatggggctgcgttcagtgtgccgagtgtgacggttaatttcacggcagatcctattccagatgctggccgct
ccagtttcaccgtctccacaccggatatcttggctgatggcacgatgagttccacattatcctttgtccctgtcgataa
gaatggccattttatcagtgggatgcagggcttgagttttactcaaaacggtgtgccggtgagtattagccccattacc
gagcagccagatagctataccgcgacggtggttgggaatagtgtcggtgatgtcacaatcacgccgcaggttgataccc
tgatactgagtacattgcagaaaaaaatatccctattcccggtacctacgctgaccggtattctggttaacgggcaaaa
tttcgctacggataaagggttcccgaaaacgatctttaaaaacgccacattccagttacagatggataacgatgttgct
aataatactcagtatgagtggtcgtcgtcattcacacccaatgtatcggttaacgatcagggtcaggtgacgattacct
accaaacctatagcgaagtggctgtgacggcgaaaagtaaaaaattcccaagttattcggtgagttatcggttctaccc
aaatcggtggatatacgatggcggcagatcgctggtatccagtctcgaggccagcagacaatgccaaggttcagatatg
tctgcggttcttgaatcctcacgtgcaaccaacggaacgcgtgcgcctgacgggacattgtggggcgagtgggggagct
tgaccgcgtatagttctgattggcaatctggtgaatattgggtcaaaaagaccagcacggattttgaaaccatgaatat
ggacacaggcgcactgcaaccagggcctgcatacttggcgttcccgctctgtgcgctgtcaatataatactagagaaag
aggagaaatactagatgtcgaataacgctttacaaacaattattaacgcccggttaccaggcgaagaggggctgtggca
gattcatctgcaggacggaaaaatcagcgccattgatgcgcaatccggcgtgatgcccataactgaaaacagcctggat
gccgaacaaggtttagttataccgccgtttgtggagccacatattcacctggacaccacgcaaaccgccggacaaccga
actggaatcagtccggcacgctgtttgaaggcattgaacgctgggccgagcgcaaagcgttattaacccatgacgatgt
gaaacaacgcgcatggcaaacgctgaaatggcagattgccaacggcattcagcatgtgcgtacccatgtcgatgtttcg
gatgcaacgctaactgcgctgaaagcaatgctggaagtgaagcaggaagtcgcgccgtggattgatctgcaaatcgtcg
ccttccctcaggaagggattttgtcgtatcccaacggtgaagcgttgctggaagaggcgttacgcttaggggcagatgt
agtgggggcgattccgcattttgaatttacccgtgaatacggcgtggagtcgctgcataaaaccttcgccctggcgcaa
aaatacgaccgtctcatcgacgttcactgtgatgagatcgatgacgagcagtcgcgctttgtcgaaaccgttgctgccc
tggcgcaccatgaaggcatgggcgcgcgagtcaccgccagccacaccacggcaatgcactcctataacggggcgtatac
ctcacgcctgttccgcttgctgaaaatgtccggtattaactttgtcgccaacccgctggtcaatattcatctgcaagga
cgtttcgatacgtatccaaaacgtcgcggcatcacgcgcgttaaagagatgctggagtccggcattaacgtctgctttg
gtcacgatgatgtcttcgatccgtggtatccgctgggaacggcgaatatgctgcaagtgctgcatatggggctgcatgt
ttgccagttgatgggctacgggcagattaacgatggcctgaatttaatcacccaccacagcgcaaggacgttgaatttg
caggattacggcattgccgccggaaacagcgccaacctgattatcctgccggctgaaaatgggtttgatgcgctgcgcc
gtcaggttccggtacgttattcggtacgtggcggcaaggtgattgccagcacacaaccggcacaaaccaccgtatatct
ggagcagccagaagccatcgattacaaacgttgatactagagaaagaggagaaatactagatggtgagcaagggcgagg
agctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtgaacggccacaagttcagcgtgtccggcga
gggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggccc
accctcgtgaccaccctgacctggggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttca
agtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccga
ggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctg
gggcacaagctggagtacaactacatcagccacaacgtctatatcaccgccgacaagcagaagaacggcatcaaggcca
acttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcga
cggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgat
cacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaataatactaga
gccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctc
tctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata