##gff-version 3
##sequence-region BBa_J32005 1 4028
BBa_J32005	.	ribosome_entry_site	2035	2046	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_J32005	.	conserved_region	2039	2042	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J32005	.	ribosome_entry_site	63	74	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_J32005	.	conserved_region	67	70	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J32005	.	terminator	3900	4028	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_J32005	.	terminator	3988	4028	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_J32005	.	engineered_region	3988	4028	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J32005	.	stem_loop	3995	4014	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J32005	.	stop_codon	4021	4021	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J32005	.	polyA_site	4015	4028	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J32005	.	terminator	3900	3979	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_J32005	.	engineered_region	3900	3979	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J32005	.	stem_loop	3911	3954	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J32005	.	terminator	2280	2408	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_J32005	.	terminator	2368	2408	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_J32005	.	engineered_region	2368	2408	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J32005	.	stem_loop	2375	2394	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J32005	.	stop_codon	2401	2401	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J32005	.	polyA_site	2395	2408	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J32005	.	terminator	2280	2359	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_J32005	.	engineered_region	2280	2359	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J32005	.	stem_loop	2291	2334	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J32005	.	terminator	732	860	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_J32005	.	terminator	820	860	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_J32005	.	engineered_region	820	860	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J32005	.	stem_loop	827	846	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J32005	.	stop_codon	853	853	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J32005	.	polyA_site	847	860	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J32005	.	terminator	732	811	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_J32005	.	engineered_region	732	811	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J32005	.	stem_loop	743	786	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J32005	.	CDS	2053	2271	.	+	0	ID=BBa_J32004;Name=BBa_J32004
BBa_J32005	.	engineered_region	2053	2271	.	+	0	ID=annotation1884532;Name=J32004;Parent=BBa_J32004
BBa_J32005	.	ribosome_entry_site	3160	3171	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_J32005	.	conserved_region	3164	3167	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J32005	.	engineered_region	3178	3891	.	+	0	ID=BBa_J06504;Name=BBa_J06504
BBa_J32005	.	sequence_alteration	3529	3529	.	+	0	ID=annotation1585833;Name=C->T (removing PstI site);Parent=BBa_J06504
BBa_J32005	.	CDS	3178	3888	.	+	0	ID=annotation1585829;Name=mCherry;Parent=BBa_J06504
BBa_J32005	.	CDS	81	698	.	+	0	ID=BBa_C0061;Name=BBa_C0061
BBa_J32005	.	CDS	81	659	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_J32005	.	engineered_tag	699	723	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_J32005	.	tag	660	691	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_J32005	.	engineered_region	81	698	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_J32005	.	engineered_region	869	2026	.	+	0	ID=BBa_F2621;Name=BBa_F2621
BBa_J32005	.	CDS	1046	1801	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_F2621
BBa_J32005	.	CDS	1046	1795	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_J32005	.	engineered_region	1046	1801	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_J32005	.	engineered_region	1046	1047	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_J32005	.	point_mutation	1537	1537	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_J32005	.	engineered_tag	1802	1826	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_J32005	.	point_mutation	1219	1219	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_J32005	.	promoter	869	1019	.	+	0	ID=BBa_R0063;Name=BBa_R0063;Parent=BBa_F2621
BBa_J32005	.	non_covalent_binding_site	869	888	.	+	0	ID=annotation2051;Name=LuxR/HSL;Parent=BBa_R0063
BBa_J32005	.	engineered_region	869	1019	.	+	0	ID=annotation7071;Name=BBa_R0063;Parent=BBa_R0063
BBa_J32005	.	start_codon	996	996	.	+	0	ID=annotation2054;Name=start;Parent=BBa_R0063
BBa_J32005	.	promoter	983	990	.	+	0	ID=annotation2052;Name=-10;Parent=BBa_R0063
BBa_J32005	.	promoter	957	962	.	+	0	ID=annotation2053;Name=-35;Parent=BBa_R0063
BBa_J32005	.	non_covalent_binding_site	998	1017	.	+	0	ID=annotation2055;Name=Putative LuxR/HSL;Parent=BBa_R0063
BBa_J32005	.	ribosome_entry_site	1028	1039	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_F2621
BBa_J32005	.	conserved_region	1032	1035	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J32005	.	terminator	1923	1963	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_F2621
BBa_J32005	.	engineered_region	1923	1963	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J32005	.	stem_loop	1930	1949	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J32005	.	stop_codon	1956	1956	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J32005	.	polyA_site	1950	1963	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J32005	.	terminator	1835	1914	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_F2621
BBa_J32005	.	engineered_region	1835	1914	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J32005	.	stem_loop	1846	1889	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J32005	.	promoter	1972	2026	.	+	0	ID=BBa_R0062;Name=BBa_R0062;Parent=BBa_F2621
BBa_J32005	.	non_covalent_binding_site	1972	1991	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_J32005	.	engineered_region	1972	2026	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_J32005	.	promoter	2013	2018	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_J32005	.	start_codon	2024	2024	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_J32005	.	promoter	1991	1996	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_J32005	.	promoter	2417	2470	.	+	0	ID=BBa_R0011;Name=BBa_R0011
BBa_J32005	.	operator	2442	2458	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_J32005	.	operator	2419	2435	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_J32005	.	promoter	2459	2464	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_J32005	.	engineered_region	2417	2470	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_J32005	.	promoter	2436	2441	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_J32005	.	ribosome_entry_site	2480	2491	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_J32005	.	conserved_region	2484	2487	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J32005	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_J32005	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_J32005	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_J32005	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_J32005	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_J32005	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_J32005	.	CDS	2498	3151	.	+	0	ID=BBa_J32026;Name=BBa_J32026
>BBa_J32005
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatacta
gatgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttat
caagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatg
cagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatat
gctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgtttt
gctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaac
acgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagt
tccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaat
gaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgct
agtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgt
tgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagaga
cctgtacgatcctacaggtgcttatgttaagtaattgtattcccagcgatacaatagtgtgacaaaaatccaatttatt
agaatcaaatgtcaatccattaccgttttaatgatatataacacgcaaaacttgcgacaaacaataggtaatactagag
aaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaa
gcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcattta
tcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgct
aatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaaca
atgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctat
tcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttattttta
catgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaat
caaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaa
aatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgc
caaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagt
gtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgt
ttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacct
gtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaatactagagaaagaggagaaatactagat
gaagcagcgtattacagtgacagttgacagcgacagctatcagttgctcaaggcatatgatgtcaatatctccggtctg
gtaagcacaaccatgcagaatgaagcccgtcgtctgcgtgccgaacgctggaaagcggaaaatcaggaagggatggctg
aggtcgcccggtttattgaaatgaacggctcttttgctgacgagaacagggactggtgatactagagccaggcatcaaa
taaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtca
cactggctcaccttcgggtgggcctttctgcgtttatatactagagaattgtgagcggataacaattgacattgtgagc
ggataacaagatactgagcacatactagagaaagaggagaaatactagatgaccatgattacgccaagctatttaggtg
acactatagaatactcaagctatgcatcaagcttggtaccgagctcggatccgctagtaacggccgccagtgtgctgga
gttctgtagatatccatcacactggagaccgctcgagcatgcatccagagggcccaattcgccctatagtgagtcgtat
tacaattcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcac
atccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctatacgt
acggcagtttaaggtttacacctataaaagagagagccgttatcgtctgtttgtggatgtacagagtgatattattgac
acgccggggcgacggatggtgatccccctggccagtgcacgtctgctgtcagataaagtctcccgtgaactttacccgg
tggtgcatatcggggatgaaagctggcgcatgatgaccaccgatatggccagtgtgccggtctccgttatcggggaaga
agtggctgatctcagccaccgcgaaaatgacatcaaaaacgccattaacctgatgttctggggaatataatactagaga
aagaggagaaatactagatggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggt
gcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccag
accgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggct
ccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcg
cgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaag
gtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccg
agcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacga
cgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggac
atcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatgg
acgagctgtacaagtaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgtt
ttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata