LOCUS       BBa_J32008              1000 bp    DNA     linear       22-Dec-2024
DEFINITION  Sagar
ACCESSION   BBa_J32008
VERSION     BBa_J32008.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     misc_feature    85..101
                     /label=lac O1
     promoter        102..107
                     /label=-35
     misc_feature    108..124
                     /label=lac O1
     promoter        125..130
                     /label=-10
     misc_feature    83..136
                     /label=BBa_R0011
     promoter        83..136
                     /label=BBa_R0011
     CDS             144..863
                     /label=GFP protein
     CDS             144..863
                     /label=BBa_E0040
     stem_loop       883..926
                     /label=stem_loop
     misc_feature    872..951
                     /label=BBa_B0010
     terminator      872..951
                     /label=BBa_B0010
     stem_loop       967..986
                     /label=T7 TE
     misc_feature    993..993
                     /label=stop
     polyA_site      987..1000
                     /label=polya
     misc_feature    960..1000
                     /label=BBa_B0012
     terminator      960..1000
                     /label=BBa_B0012
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag agaattgtga gcggataaca attgacattg tgagcggata
      121 acaagatact gagcacatac tagatgcgta aaggagaaga acttttcact ggagttgtcc
      181 caattcttgt tgaattagat ggtgatgtta atgggcacaa attttctgtc agtggagagg
      241 gtgaaggtga tgcaacatac ggaaaactta cccttaaatt tatttgcact actggaaaac
      301 tacctgttcc atggccaaca cttgtcacta ctttcggtta tggtgttcaa tgctttgcga
      361 gatacccaga tcatatgaaa cagcatgact ttttcaagag tgccatgccc gaaggttatg
      421 tacaggaaag aactatattt ttcaaagatg acgggaacta caagacacgt gctgaagtca
      481 agtttgaagg tgataccctt gttaatagaa tcgagttaaa aggtattgat tttaaagaag
      541 atggaaacat tcttggacac aaattggaat acaactataa ctcacacaat gtatacatca
      601 tggcagacaa acaaaagaat ggaatcaaag ttaacttcaa aattagacac aacattgaag
      661 atggaagcgt tcaactagca gaccattatc aacaaaatac tccaattggc gatggccctg
      721 tccttttacc agacaaccat tacctgtcca cacaatctgc cctttcgaaa gatcccaacg
      781 aaaagagaga ccacatggtc cttcttgagt ttgtaacagc tgctgggatt acacatggca
      841 tggatgaact atacaaataa taatactaga gccaggcatc aaataaaacg aaaggctcag
      901 tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt
      961 cacactggct caccttcggg tgggcctttc tgcgtttata
//