LOCUS BBa_J32011 5518 bp DNA linear 22-Nov-2024 DEFINITION X-Verter Receiver Reporter ACCESSION BBa_J32011 VERSION BBa_J32011.1 FEATURES Location/Qualifiers misc_feature 1..20 /label=LuxR/HSL promoter 20..25 /label=-35 promoter 42..47 /label=-10 misc_feature 53..53 /label=start misc_feature 1..55 /label=BBa_R0062 promoter 1..55 /label=BBa_R0062 misc_feature 68..71 /label=conserved RBS 64..75 /label=BBa_B0034 CDS 82..804 /label=rhlR misc_feature 805..837 /label=LVA misc_feature 844..868 /label=Help:Barcodes CDS 82..868 /label=BBa_C0071 misc_feature 881..884 /label=conserved RBS 877..888 /label=BBa_B0034 CDS 895..1602 /label=mCherry misc_feature 1246..1246 /label=C->T (removing PstI site) misc_feature 1603..1611 /label=LVA misc_feature 895..1617 /label=BBa_J06505 stem_loop 1637..1680 /label=stem_loop misc_feature 1626..1705 /label=BBa_B0010 terminator 1626..1705 /label=BBa_B0010 stem_loop 1721..1740 /label=T7 TE misc_feature 1747..1747 /label=stop polyA_site 1741..1754 /label=polya misc_feature 1714..1754 /label=BBa_B0012 terminator 1714..1754 /label=BBa_B0012 terminator 1626..1754 /label=BBa_B0015 misc_feature 1763..1782 /label=RhlR promoter 1781..1786 /label=-35 promoter 1804..1809 /label=-10 misc_feature 1815..1815 /label=start promoter 1763..1815 /label=BBa_R0071 misc_feature 1828..1831 /label=conserved RBS 1824..1835 /label=BBa_B0034 CDS 1842..2564 /label=cinR misc_feature 2565..2597 /label=LVA misc_feature 2604..2628 /label=Help:Barcodes CDS 1842..2628 /label=BBa_C0077 misc_feature 2641..2644 /label=conserved RBS 2637..2648 /label=BBa_B0034 CDS 2655..3416 /label=CFP (LVA) misc_feature 3373..3410 /label=SsrA misc_feature 3411..3416 /label=2 misc_feature 2723..2723 /label=A misc_feature 2655..3416 /label=BBa_E0022 misc_feature 2655..3416 /label=BBa_E0022 stem_loop 3436..3479 /label=stem_loop misc_feature 3425..3504 /label=BBa_B0010 terminator 3425..3504 /label=BBa_B0010 stem_loop 3520..3539 /label=T7 TE misc_feature 3546..3546 /label=stop polyA_site 3540..3553 /label=polya misc_feature 3513..3553 /label=BBa_B0012 terminator 3513..3553 /label=BBa_B0012 terminator 3425..3553 /label=BBa_B0015 misc_feature 3562..3781 /label=unidentified/uncharacterized CinR dependent stem_loop 3596..3635 /label=stem_loop stem_loop 3652..3697 /label=stem_loop promoter 3562..3786 /label=BBa_R0078 misc_feature 3799..3802 /label=conserved RBS 3795..3806 /label=BBa_B0034 misc_feature 3813..3814 /label=prefix misc_feature 3986..3986 /label=T misc_feature 4304..4304 /label=A CDS 3813..4562 /label=luxR misc_feature 3813..4568 /label=BBa_C0062 misc_feature 4569..4593 /label=Help:Barcodes CDS 3813..4568 /label=BBa_C0062 misc_feature 4606..4609 /label=conserved RBS 4602..4613 /label=BBa_B0034 CDS 4620..5381 /label=YFP (LVA) misc_feature 5376..5381 /label=2 misc_feature 5337..5375 /label=SsrA misc_feature 4861..4861 /label=A (Q->L) misc_feature 4620..5381 /label=BBa_E0032 misc_feature 4620..5381 /label=BBa_E0032 stem_loop 5401..5444 /label=stem_loop misc_feature 5390..5469 /label=BBa_B0010 terminator 5390..5469 /label=BBa_B0010 stem_loop 5485..5504 /label=T7 TE misc_feature 5511..5511 /label=stop polyA_site 5505..5518 /label=polya misc_feature 5478..5518 /label=BBa_B0012 terminator 5478..5518 /label=BBa_B0012 terminator 5390..5518 /label=BBa_B0015 ORIGIN 1 acctgtagga tcgtacaggt ttacgcaaga aaatggtttg ttatagtcga ataaatacta 61 gagaaagagg agaaatacta gatgaggaat gacggaggct ttttgctgtg gtgggacggt 121 ttgcgtagcg agatgcagcc gatccacgac agccagggcg tgttcgccgt cctggaaaag 181 gaagtgcggc gcctgggctt cgattactac gcctatggcg tgcgccacac gattcccttc 241 acccggccga agaccgaggt ccatggcacc tatcccaagg cctggctgga gcgataccag 301 atgcagaact acggggccgt ggatccggcg atcctcaacg gcctgcgctc ctcggaaatg 361 gtggtctgga gcgacagcct gttcgaccag agccggatgc tctggaacga ggctcgcgat 421 tggggcctct gtgtcggcgc gaccttgccg atccgcgcgc cgaacaattt gctcagcgtg 481 ctttccgtgg cgcgcgacca gcagaacatc tccagcttcg agcgcgagga aatccgcctg 541 cggctgcgtt gcatgatcga gttgctgacc cagaagctga ccgacctgga gcatccgatg 601 ctgatgtcca acccggtctg cctgagccat cgcgaacgcg agatcctgca atggaccgcc 661 gacggcaaga gttccgggga aatcgccatc atcctgagca tctccgagag cacggtgaac 721 ttccaccaca agaacatcca gaagaagttc gacgcgccga acaagacgct ggctgccgcc 781 tacgccgcgg cgctgggtct catcgctgca aacgacgaaa actacgcttt agtagcttaa 841 taacactgat agtgctagtg tagatcacta ctagagaaag aggagaaata ctagatggtg 901 agcaagggcg aggaggataa catggccatc atcaaggagt tcatgcgctt caaggtgcac 961 atggagggct ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc 1021 tacgagggca cccagaccgc caagctgaag gtgaccaagg gtggccccct gcccttcgcc 1081 tgggacatcc tgtcccctca gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc 1141 gacatccccg actacttgaa gctgtccttc cccgagggct tcaagtggga gcgcgtgatg 1201 aacttcgagg acggcggcgt ggtgaccgtg acccaggact cctccttgca ggacggcgag 1261 ttcatctaca aggtgaagct gcgcggcacc aacttcccct ccgacggccc cgtaatgcag 1321 aagaagacca tgggctggga ggcctcctcc gagcggatgt accccgagga cggcgccctg 1381 aagggcgaga tcaagcagag gctgaagctg aaggacggcg gccactacga cgctgaggtc 1441 aagaccacct acaaggccaa gaagcccgtg cagctgcccg gcgcctacaa cgtcaacatc 1501 aagttggaca tcacctccca caacgaggac tacaccatcg tggaacagta cgaacgcgcc 1561 gagggccgcc actccaccgg cggcatggac gagctgtaca agttagtagc ttaataatac 1621 tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat 1681 ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc 1741 tttctgcgtt tatatactag agtcctgtga aatctggcag ttaccgttag ctttcgaatt 1801 ggctaaaaag tgttctacta gagaaagagg agaaatacta gatgattgag aatacctata 1861 gcgaaaagtt cgagtccgcg ttcgaacaga tcaaggcggc ggccaacgtg gatgccgcca 1921 tccgtattct ccaggcggaa tataacctcg atttcgtcac ctaccatctc gcccagacga 1981 tcgcgagcaa gatcgattcg cccttcgtgc gcaccaccta tccggatgcc tgggtttccc 2041 gctacctcct caacagctat gtgaaggtcg atccgatcgt caagcagggc ttcgaacgcc 2101 agctgccctt cgactggagc gaggtcgaac cgacgccgga ggcctatgcc atgctggtcg 2161 acgcccagaa acacggcatc ggtggcaatg gctactccat ccccgtcgcc gacaaggcgc 2221 agcgccgcgc cctgctgtcg ctgaatgccc gtataccggc cgacgaatgg accgagctcg 2281 tgcgccgctg ccgcaacgag tggatcgaga tcgcccatct gatccaccgc aaggccgtct 2341 atgagctgca tggcgaaaac gatccggtgc cggcattgtc gccgcgcgag atcgagtgtc 2401 tgcactggac cgccctcggc aaggattaca aggatatttc ggtcatcctg ggcatatcag 2461 agcataccac acgcgattac ctgaagaccg cccgcttcaa gctcggctgc gccacgatct 2521 cggccgccgc gtcgcgggct gttcaattgc gcatcatcaa tcccgctgca aacgacgaaa 2581 actacgcttt agtagcttaa taacgctgat agtgctagtg tagatcgcta ctagagaaag 2641 aggagaaata ctagatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc 2701 tggtcgagct ggacggcgac gtgaacggcc acaagttcag cgtgtccggc gagggcgagg 2761 gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg 2821 tgccctggcc caccctcgtg accaccctga cctggggcgt gcagtgcttc agccgctacc 2881 ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg 2941 agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg 3001 agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca 3061 acatcctggg gcacaagctg gagtacaact acatcagcca caacgtctat atcaccgccg 3121 acaagcagaa gaacggcatc aaggccaact tcaagatccg ccacaacatc gaggacggca 3181 gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc 3241 tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc 3301 gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg 3361 agctgtacaa gaggcctgct gcaaacgacg aaaactacgc tttagtagct taataatact 3421 agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc 3481 tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct 3541 ttctgcgttt atatactaga gccctttgtg cgtccaaacg gacgcacggc gctctaaagc 3601 gggtcgcgat ctttcagatt cgctcctcgc gctttcagtc tttgttttgg cgcatgtcgt 3661 tatcgcaaaa ccgctgcaca cttttgcgcg acatgctctg atccccctca tctggggggg 3721 cctatctgag ggaatttccg atccggctcg cctgaaccat tctgctttcc acgaacttga 3781 aaacgctact agagaaagag gagaaatact agatgaaaaa cataaatgcc gacgacacat 3841 acagaataat taataaaatt aaagcttgta gaagcaataa tgatattaat caatgcttat 3901 ctgatatgac taaaatggta cattgtgaat attatttact cgcgatcatt tatcctcatt 3961 ctatggttaa atctgatatt tcaatcctag ataattaccc taaaaaatgg aggcaatatt 4021 atgatgacgc taatttaata aaatatgatc ctatagtaga ttattctaac tccaatcatt 4081 caccaattaa ttggaatata tttgaaaaca atgctgtaaa taaaaaatct ccaaatgtaa 4141 ttaaagaagc gaaaacatca ggtcttatca ctgggtttag tttccctatt catacggcta 4201 acaatggctt cggaatgctt agttttgcac attcagaaaa agacaactat atagatagtt 4261 tatttttaca tgcgtgtatg aacataccat taattgttcc ttctctagtt gataattatc 4321 gaaaaataaa tatagcaaat aataaatcaa acaacgattt aaccaaaaga gaaaaagaat 4381 gtttagcgtg ggcatgcgaa ggaaaaagct cttgggatat ttcaaaaata ttaggttgca 4441 gtgagcgtac tgtcactttc catttaacca atgcgcaaat gaaactcaat acaacaaacc 4501 gctgccaaag tatttctaaa gcaattttaa caggagcaat tgattgccca tactttaaaa 4561 attaataaca ctgatagtgc tagtgtagat cactactaga gaaagaggag aaatactaga 4621 tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg 4681 gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg 4741 gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc 4801 tcgtgaccac cttcggctac ggcctgcaat gcttcgcccg ctaccccgac cacatgaagc 4861 tgcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct 4921 tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg 4981 tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca 5041 agctggagta caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg 5101 gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg 5161 accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact 5221 acctgagcta ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc 5281 tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagaggc 5341 ctgctgcaaa cgacgaaaac tacgctttag tagcttaata atactagagc caggcatcaa 5401 ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg 5461 aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg cgtttata //