##gff-version 3
##sequence-region BBa_J32011 1 5518
BBa_J32011	.	ribosome_entry_site	1824	1835	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_J32011	.	conserved_region	1828	1831	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J32011	.	terminator	5390	5518	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_J32011	.	terminator	5390	5469	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_J32011	.	stem_loop	5401	5444	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J32011	.	engineered_region	5390	5469	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J32011	.	terminator	5478	5518	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_J32011	.	engineered_region	5478	5518	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J32011	.	stop_codon	5511	5511	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J32011	.	stem_loop	5485	5504	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J32011	.	polyA_site	5505	5518	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J32011	.	terminator	3425	3553	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_J32011	.	terminator	3425	3504	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_J32011	.	stem_loop	3436	3479	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J32011	.	engineered_region	3425	3504	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J32011	.	terminator	3513	3553	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_J32011	.	engineered_region	3513	3553	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J32011	.	stop_codon	3546	3546	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J32011	.	stem_loop	3520	3539	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J32011	.	polyA_site	3540	3553	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J32011	.	ribosome_entry_site	64	75	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_J32011	.	conserved_region	68	71	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J32011	.	engineered_region	4620	5381	.	+	0	ID=BBa_E0032;Name=BBa_E0032
BBa_J32011	.	CDS	4620	5381	.	+	0	ID=annotation2156;Name=YFP (LVA);Parent=BBa_E0032
BBa_J32011	.	tag	5337	5375	.	+	0	ID=annotation2160;Name=SsrA;Parent=BBa_E0032
BBa_J32011	.	engineered_region	4620	5381	.	+	0	ID=annotation7041;Name=BBa_E0032;Parent=BBa_E0032
BBa_J32011	.	stop_codon	5376	5381	.	+	0	ID=annotation2159;Name=2;Parent=BBa_E0032
BBa_J32011	.	sequence_alteration	4861	4861	.	+	0	ID=annotation2161;Name=A (Q->L);Parent=BBa_E0032
BBa_J32011	.	ribosome_entry_site	877	888	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_J32011	.	conserved_region	881	884	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J32011	.	ribosome_entry_site	3795	3806	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_J32011	.	conserved_region	3799	3802	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J32011	.	terminator	1626	1754	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_J32011	.	terminator	1626	1705	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_J32011	.	stem_loop	1637	1680	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J32011	.	engineered_region	1626	1705	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J32011	.	terminator	1714	1754	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_J32011	.	engineered_region	1714	1754	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J32011	.	stop_codon	1747	1747	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J32011	.	stem_loop	1721	1740	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J32011	.	polyA_site	1741	1754	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J32011	.	engineered_region	2655	3416	.	+	0	ID=BBa_E0022;Name=BBa_E0022
BBa_J32011	.	CDS	2655	3416	.	+	0	ID=annotation2150;Name=CFP (LVA);Parent=BBa_E0022
BBa_J32011	.	tag	3373	3410	.	+	0	ID=annotation2153;Name=SsrA;Parent=BBa_E0022
BBa_J32011	.	engineered_region	2655	3416	.	+	0	ID=annotation7040;Name=BBa_E0022;Parent=BBa_E0022
BBa_J32011	.	point_mutation	2723	2723	.	+	0	ID=annotation2155;Name=A;Parent=BBa_E0022
BBa_J32011	.	stop_codon	3411	3416	.	+	0	ID=annotation2154;Name=2;Parent=BBa_E0022
BBa_J32011	.	CDS	1842	2628	.	+	0	ID=BBa_C0077;Name=BBa_C0077
BBa_J32011	.	tag	2565	2597	.	+	0	ID=annotation306600;Name=LVA;Parent=BBa_C0077
BBa_J32011	.	engineered_tag	2604	2628	.	+	0	ID=annotation2214000;Name=Help:Barcodes;Parent=BBa_C0077
BBa_J32011	.	CDS	1842	2564	.	+	0	ID=annotation301112;Name=cinR;Parent=BBa_C0077
BBa_J32011	.	promoter	1763	1815	.	+	0	ID=BBa_R0071;Name=BBa_R0071
BBa_J32011	.	promoter	1804	1809	.	+	0	ID=annotation297104;Name=-10;Parent=BBa_R0071
BBa_J32011	.	start_codon	1815	1815	.	+	0	ID=annotation297105;Name=start;Parent=BBa_R0071
BBa_J32011	.	non_covalent_binding_site	1763	1782	.	+	0	ID=annotation297102;Name=RhlR;Parent=BBa_R0071
BBa_J32011	.	promoter	1781	1786	.	+	0	ID=annotation297103;Name=-35;Parent=BBa_R0071
BBa_J32011	.	CDS	82	868	.	+	0	ID=BBa_C0071;Name=BBa_C0071
BBa_J32011	.	engineered_tag	844	868	.	+	0	ID=annotation2213995;Name=Help:Barcodes;Parent=BBa_C0071
BBa_J32011	.	CDS	82	804	.	+	0	ID=annotation301192;Name=rhlR;Parent=BBa_C0071
BBa_J32011	.	tag	805	837	.	+	0	ID=annotation306523;Name=LVA;Parent=BBa_C0071
BBa_J32011	.	ribosome_entry_site	2637	2648	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_J32011	.	conserved_region	2641	2644	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J32011	.	promoter	3562	3786	.	+	0	ID=BBa_R0078;Name=BBa_R0078
BBa_J32011	.	stem_loop	3652	3697	.	+	0	ID=annotation318250;Name=stem_loop;Parent=BBa_R0078
BBa_J32011	.	stem_loop	3596	3635	.	+	0	ID=annotation318244;Name=stem_loop;Parent=BBa_R0078
BBa_J32011	.	sequence_feature	3562	3781	.	+	0	ID=annotation318234;Name=unidentified/uncharacterized CinR dependent;Parent=BBa_R0078
BBa_J32011	.	engineered_region	895	1617	.	+	0	ID=BBa_J06505;Name=BBa_J06505
BBa_J32011	.	CDS	895	1602	.	+	0	ID=annotation1585879;Name=mCherry;Parent=BBa_J06505
BBa_J32011	.	tag	1603	1611	.	+	0	ID=annotation1585887;Name=LVA;Parent=BBa_J06505
BBa_J32011	.	sequence_alteration	1246	1246	.	+	0	ID=annotation1585880;Name=C->T (removing PstI site);Parent=BBa_J06505
BBa_J32011	.	promoter	1	55	.	+	0	ID=BBa_R0062;Name=BBa_R0062
BBa_J32011	.	non_covalent_binding_site	1	20	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_J32011	.	promoter	42	47	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_J32011	.	engineered_region	1	55	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_J32011	.	start_codon	53	53	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_J32011	.	promoter	20	25	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_J32011	.	CDS	3813	4568	.	+	0	ID=BBa_C0062;Name=BBa_C0062
BBa_J32011	.	CDS	3813	4562	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_J32011	.	engineered_region	3813	4568	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_J32011	.	engineered_tag	4569	4593	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_J32011	.	point_mutation	3986	3986	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_J32011	.	engineered_region	3813	3814	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_J32011	.	point_mutation	4304	4304	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_J32011	.	ribosome_entry_site	4602	4613	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_J32011	.	conserved_region	4606	4609	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
>BBa_J32011
acctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaatactagagaaagaggagaaatact
agatgaggaatgacggaggctttttgctgtggtgggacggtttgcgtagcgagatgcagccgatccacgacagccaggg
cgtgttcgccgtcctggaaaaggaagtgcggcgcctgggcttcgattactacgcctatggcgtgcgccacacgattccc
ttcacccggccgaagaccgaggtccatggcacctatcccaaggcctggctggagcgataccagatgcagaactacgggg
ccgtggatccggcgatcctcaacggcctgcgctcctcggaaatggtggtctggagcgacagcctgttcgaccagagccg
gatgctctggaacgaggctcgcgattggggcctctgtgtcggcgcgaccttgccgatccgcgcgccgaacaatttgctc
agcgtgctttccgtggcgcgcgaccagcagaacatctccagcttcgagcgcgaggaaatccgcctgcggctgcgttgca
tgatcgagttgctgacccagaagctgaccgacctggagcatccgatgctgatgtccaacccggtctgcctgagccatcg
cgaacgcgagatcctgcaatggaccgccgacggcaagagttccggggaaatcgccatcatcctgagcatctccgagagc
acggtgaacttccaccacaagaacatccagaagaagttcgacgcgccgaacaagacgctggctgccgcctacgccgcgg
cgctgggtctcatcgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcact
actagagaaagaggagaaatactagatggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgc
ttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagg
gcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcat
gtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaag
tgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttca
tctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggc
ctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggc
cactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatca
agttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccgg
cggcatggacgagctgtacaagttagtagcttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaa
agactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtggg
cctttctgcgtttatatactagagtcctgtgaaatctggcagttaccgttagctttcgaattggctaaaaagtgttcta
ctagagaaagaggagaaatactagatgattgagaatacctatagcgaaaagttcgagtccgcgttcgaacagatcaagg
cggcggccaacgtggatgccgccatccgtattctccaggcggaatataacctcgatttcgtcacctaccatctcgccca
gacgatcgcgagcaagatcgattcgcccttcgtgcgcaccacctatccggatgcctgggtttcccgctacctcctcaac
agctatgtgaaggtcgatccgatcgtcaagcagggcttcgaacgccagctgcccttcgactggagcgaggtcgaaccga
cgccggaggcctatgccatgctggtcgacgcccagaaacacggcatcggtggcaatggctactccatccccgtcgccga
caaggcgcagcgccgcgccctgctgtcgctgaatgcccgtataccggccgacgaatggaccgagctcgtgcgccgctgc
cgcaacgagtggatcgagatcgcccatctgatccaccgcaaggccgtctatgagctgcatggcgaaaacgatccggtgc
cggcattgtcgccgcgcgagatcgagtgtctgcactggaccgccctcggcaaggattacaaggatatttcggtcatcct
gggcatatcagagcataccacacgcgattacctgaagaccgcccgcttcaagctcggctgcgccacgatctcggccgcc
gcgtcgcgggctgttcaattgcgcatcatcaatcccgctgcaaacgacgaaaactacgctttagtagcttaataacgct
gatagtgctagtgtagatcgctactagagaaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccgg
ggtggtgcccatcctggtcgagctggacggcgacgtgaacggccacaagttcagcgtgtccggcgagggcgagggcgat
gccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgacca
ccctgacctggggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcc
cgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgag
ggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctgg
agtacaactacatcagccacaacgtctatatcaccgccgacaagcagaagaacggcatcaaggccaacttcaagatccg
ccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctg
ctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgc
tggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagaggcctgctgcaaacgacgaaaacta
cgctttagtagcttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttt
tatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatata
ctagagccctttgtgcgtccaaacggacgcacggcgctctaaagcgggtcgcgatctttcagattcgctcctcgcgctt
tcagtctttgttttggcgcatgtcgttatcgcaaaaccgctgcacacttttgcgcgacatgctctgatccccctcatct
gggggggcctatctgagggaatttccgatccggctcgcctgaaccattctgctttccacgaacttgaaaacgctactag
agaaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtag
aagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatt
tatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacg
ctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaa
caatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccct
attcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttt
tacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataa
atcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttca
aaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgct
gccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgcta
gtgtagatcactactagagaaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccggggtggtgccc
atcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacg
gcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggcta
cggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaaggctac
gtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccc
tggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaacta
caacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatc
gaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgaca
accactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgt
gaccgccgccgggatcactctcggcatggacgagctgtacaagaggcctgctgcaaacgacgaaaactacgctttagta
gcttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgt
ttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata