X-Verter R
type: ComponentDefinition
persistent identity: BBa J32011
display id: BBa_J32011
version: 1
was generated by: iGEM to SBOL conversion
description: X-Verter Receiver Reporter
created: 2006-07-21T11:00:00Z
modified: 2015-08-31T04:08:46Z
mutable provenance: a
owned by: james
owned by: myers
mutable description: a
discontinued: false
dominant: false
experience: experience/None
group u list: _
m user id: 0
owner id: 495
owning group id: 50
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: a
star: false
creator: Sagar Indurkhya and Austen Heinz
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0015
component: BBa_E0032
component: BBa_B0034
component: BBa_R0078
component: BBa_C0062
component: BBa_C0077
component: BBa_E0022
component: BBa_J06505
component: BBa_R0071
component: BBa_R0062
component: BBa_C0071
sequence annotation: BBa_R0062
sequence annotation: BBa_B0034
sequence annotation: BBa_C0071
sequence annotation: BBa_J06505
sequence annotation: BBa_B0015
sequence annotation: BBa_R0071
sequence annotation: BBa_C0077
sequence annotation: BBa_E0022
sequence annotation: BBa_R0078
sequence annotation: BBa_C0062
sequence annotation: BBa_E0032
sequence: https://synbiohub.org/public/igem/BBa_J32011_sequence/1

BBa_B0015
type: Component
persistent identity: component2321297
display id: component2321297
version: 1
top level: X-Verter R
definition: BBa_B0015
access: public

BBa_E0032
type: Component
persistent identity: component2321287
display id: component2321287
version: 1
top level: X-Verter R
definition: YFP
access: public

BBa_B0034
type: Component
persistent identity: component2321277
display id: component2321277
version: 1
top level: X-Verter R
definition: BBa_B0034
access: public

BBa_R0078
type: Component
persistent identity: component2321275
display id: component2321275
version: 1
top level: X-Verter R
definition: cinR
access: public

BBa_B0034
type: Component
persistent identity: component2321285
display id: component2321285
version: 1
top level: X-Verter R
definition: BBa_B0034
access: public

BBa_C0062
type: Component
persistent identity: component2321280
display id: component2321280
version: 1
top level: X-Verter R
definition: luxr
access: public

BBa_B0034
type: Component
persistent identity: component2321259
display id: component2321259
version: 1
top level: X-Verter R
definition: BBa_B0034
access: public

BBa_C0077
type: Component
persistent identity: component2321257
display id: component2321257
version: 1
top level: X-Verter R
definition: cinr
access: public

BBa_B0015
type: Component
persistent identity: component2321271
display id: component2321271
version: 1
top level: X-Verter R
definition: BBa_B0015
access: public

BBa_E0022
type: Component
persistent identity: component2321261
display id: component2321261
version: 1
top level: X-Verter R
definition: ECFP
access: public

BBa_B0015
type: Component
persistent identity: component2321247
display id: component2321247
version: 1
top level: X-Verter R
definition: BBa_B0015
access: public

BBa_J06505
type: Component
persistent identity: component2321240
display id: component2321240
version: 1
top level: X-Verter R
definition: mCherry
access: public

BBa_B0034
type: Component
persistent identity: component2321253
display id: component2321253
version: 1
top level: X-Verter R
definition: BBa_B0034
access: public

BBa_R0071
type: Component
persistent identity: component2321251
display id: component2321251
version: 1
top level: X-Verter R
definition: RhlR+C4
access: public

BBa_B0034
type: Component
persistent identity: component2321230
display id: component2321230
version: 1
top level: X-Verter R
definition: BBa_B0034
access: public

BBa_R0062
type: Component
persistent identity: component2321224
display id: component2321224
version: 1
top level: X-Verter R
definition: lux pR
access: public

BBa_B0034
type: Component
persistent identity: component2321236
display id: component2321236
version: 1
top level: X-Verter R
definition: BBa_B0034
access: public

BBa_C0071
type: Component
persistent identity: component2321234
display id: component2321234
version: 1
top level: X-Verter R
definition: rhlR
access: public

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation2321224
display id: annotation2321224
version: 1
top level: X-Verter R
location: https://synbiohub.org/public/igem/BBa_J32011/annotation2321224/range2321224/1
component: BBa_R0062

https://synbiohub.org/public/igem/BBa_J32011/annotation2321224/range2321224/1
type: Range
persistent identity: range2321224
display id: range2321224
version: 1
top level: X-Verter R
direction: direction/forward
start: 1
end: 55
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2321230
display id: annotation2321230
version: 1
top level: X-Verter R
location: https://synbiohub.org/public/igem/BBa_J32011/annotation2321230/range2321230/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J32011/annotation2321230/range2321230/1
type: Range
persistent identity: range2321230
display id: range2321230
version: 1
top level: X-Verter R
direction: direction/reverse
start: 64
end: 75
orientation: inline

BBa_C0071
type: SequenceAnnotation
persistent identity: annotation2321234
display id: annotation2321234
version: 1
top level: X-Verter R
location: https://synbiohub.org/public/igem/BBa_J32011/annotation2321234/range2321234/1
component: BBa_C0071

https://synbiohub.org/public/igem/BBa_J32011/annotation2321234/range2321234/1
type: Range
persistent identity: range2321234
display id: range2321234
version: 1
top level: X-Verter R
direction: direction/reverse
start: 82
end: 868
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2321236
display id: annotation2321236
version: 1
top level: X-Verter R
location: https://synbiohub.org/public/igem/BBa_J32011/annotation2321236/range2321236/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J32011/annotation2321236/range2321236/1
type: Range
persistent identity: range2321236
display id: range2321236
version: 1
top level: X-Verter R
direction: direction/reverse
start: 877
end: 888
orientation: inline

BBa_J06505
type: SequenceAnnotation
persistent identity: annotation2321240
display id: annotation2321240
version: 1
top level: X-Verter R
location: https://synbiohub.org/public/igem/BBa_J32011/annotation2321240/range2321240/1
component: BBa_J06505

https://synbiohub.org/public/igem/BBa_J32011/annotation2321240/range2321240/1
type: Range
persistent identity: range2321240
display id: range2321240
version: 1
top level: X-Verter R
direction: direction/reverse
start: 895
end: 1617
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2321247
display id: annotation2321247
version: 1
top level: X-Verter R
location: https://synbiohub.org/public/igem/BBa_J32011/annotation2321247/range2321247/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_J32011/annotation2321247/range2321247/1
type: Range
persistent identity: range2321247
display id: range2321247
version: 1
top level: X-Verter R
direction: direction/reverse
start: 1626
end: 1754
orientation: inline

BBa_R0071
type: SequenceAnnotation
persistent identity: annotation2321251
display id: annotation2321251
version: 1
top level: X-Verter R
location: https://synbiohub.org/public/igem/BBa_J32011/annotation2321251/range2321251/1
component: BBa_R0071

https://synbiohub.org/public/igem/BBa_J32011/annotation2321251/range2321251/1
type: Range
persistent identity: range2321251
display id: range2321251
version: 1
top level: X-Verter R
direction: direction/reverse
start: 1763
end: 1815
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2321253
display id: annotation2321253
version: 1
top level: X-Verter R
location: https://synbiohub.org/public/igem/BBa_J32011/annotation2321253/range2321253/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J32011/annotation2321253/range2321253/1
type: Range
persistent identity: range2321253
display id: range2321253
version: 1
top level: X-Verter R
direction: direction/reverse
start: 1824
end: 1835
orientation: inline

BBa_C0077
type: SequenceAnnotation
persistent identity: annotation2321257
display id: annotation2321257
version: 1
top level: X-Verter R
location: https://synbiohub.org/public/igem/BBa_J32011/annotation2321257/range2321257/1
component: BBa_C0077

https://synbiohub.org/public/igem/BBa_J32011/annotation2321257/range2321257/1
type: Range
persistent identity: range2321257
display id: range2321257
version: 1
top level: X-Verter R
direction: direction/reverse
start: 1842
end: 2628
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2321259
display id: annotation2321259
version: 1
top level: X-Verter R
location: https://synbiohub.org/public/igem/BBa_J32011/annotation2321259/range2321259/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J32011/annotation2321259/range2321259/1
type: Range
persistent identity: range2321259
display id: range2321259
version: 1
top level: X-Verter R
direction: direction/reverse
start: 2637
end: 2648
orientation: inline

BBa_E0022
type: SequenceAnnotation
persistent identity: annotation2321261
display id: annotation2321261
version: 1
top level: X-Verter R
location: https://synbiohub.org/public/igem/BBa_J32011/annotation2321261/range2321261/1
component: BBa_E0022

https://synbiohub.org/public/igem/BBa_J32011/annotation2321261/range2321261/1
type: Range
persistent identity: range2321261
display id: range2321261
version: 1
top level: X-Verter R
direction: direction/forward
start: 2655
end: 3416
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2321271
display id: annotation2321271
version: 1
top level: X-Verter R
location: https://synbiohub.org/public/igem/BBa_J32011/annotation2321271/range2321271/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_J32011/annotation2321271/range2321271/1
type: Range
persistent identity: range2321271
display id: range2321271
version: 1
top level: X-Verter R
direction: direction/reverse
start: 3425
end: 3553
orientation: inline

BBa_R0078
type: SequenceAnnotation
persistent identity: annotation2321275
display id: annotation2321275
version: 1
top level: X-Verter R
location: https://synbiohub.org/public/igem/BBa_J32011/annotation2321275/range2321275/1
component: BBa_R0078

https://synbiohub.org/public/igem/BBa_J32011/annotation2321275/range2321275/1
type: Range
persistent identity: range2321275
display id: range2321275
version: 1
top level: X-Verter R
direction: direction/reverse
start: 3562
end: 3786
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2321277
display id: annotation2321277
version: 1
top level: X-Verter R
location: https://synbiohub.org/public/igem/BBa_J32011/annotation2321277/range2321277/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J32011/annotation2321277/range2321277/1
type: Range
persistent identity: range2321277
display id: range2321277
version: 1
top level: X-Verter R
direction: direction/reverse
start: 3795
end: 3806
orientation: inline

BBa_C0062
type: SequenceAnnotation
persistent identity: annotation2321280
display id: annotation2321280
version: 1
top level: X-Verter R
location: https://synbiohub.org/public/igem/BBa_J32011/annotation2321280/range2321280/1
component: BBa_C0062

https://synbiohub.org/public/igem/BBa_J32011/annotation2321280/range2321280/1
type: Range
persistent identity: range2321280
display id: range2321280
version: 1
top level: X-Verter R
direction: direction/forward
start: 3813
end: 4568
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2321285
display id: annotation2321285
version: 1
top level: X-Verter R
location: https://synbiohub.org/public/igem/BBa_J32011/annotation2321285/range2321285/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J32011/annotation2321285/range2321285/1
type: Range
persistent identity: range2321285
display id: range2321285
version: 1
top level: X-Verter R
direction: direction/reverse
start: 4602
end: 4613
orientation: inline

BBa_E0032
type: SequenceAnnotation
persistent identity: annotation2321287
display id: annotation2321287
version: 1
top level: X-Verter R
location: https://synbiohub.org/public/igem/BBa_J32011/annotation2321287/range2321287/1
component: BBa_E0032

https://synbiohub.org/public/igem/BBa_J32011/annotation2321287/range2321287/1
type: Range
persistent identity: range2321287
display id: range2321287
version: 1
top level: X-Verter R
direction: direction/forward
start: 4620
end: 5381
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2321297
display id: annotation2321297
version: 1
top level: X-Verter R
location: https://synbiohub.org/public/igem/BBa_J32011/annotation2321297/range2321297/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_J32011/annotation2321297/range2321297/1
type: Range
persistent identity: range2321297
display id: range2321297
version: 1
top level: X-Verter R
direction: direction/reverse
start: 5390
end: 5518
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

cinR
type: ComponentDefinition
persistent identity: BBa R0078
display id: BBa_R0078
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter (cinR and HSL regulated)
created: 2004-01-29T12:00:00Z
modified: 2015-05-08T01:14:15Z
mutable provenance: Rhizobium leguminosarum
owned by: james
owned by: myers
part status: Released HQ 2013
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Drew Endy
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: unidentified/uncharacterized CinR dependent
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_R0078_sequence/1

unidentified/uncharacterized CinR dependent
type: SequenceAnnotation
persistent identity: annotation318234
display id: annotation318234
version: 1
top level: cinR
location: https://synbiohub.org/public/igem/BBa_R0078/annotation318234/range318234/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_R0078/annotation318234/range318234/1
type: Range
persistent identity: range318234
display id: range318234
version: 1
top level: cinR
direction: direction/forward
start: 1
end: 220
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation318244
display id: annotation318244
version: 1
top level: cinR
location: https://synbiohub.org/public/igem/BBa_R0078/annotation318244/range318244/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_R0078/annotation318244/range318244/1
type: Range
persistent identity: range318244
display id: range318244
version: 1
top level: cinR
direction: direction/forward
start: 35
end: 74
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation318250
display id: annotation318250
version: 1
top level: cinR
location: https://synbiohub.org/public/igem/BBa_R0078/annotation318250/range318250/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_R0078/annotation318250/range318250/1
type: Range
persistent identity: range318250
display id: range318250
version: 1
top level: cinR
direction: direction/forward
start: 91
end: 136
orientation: inline

RhlR+C4
type: ComponentDefinition
persistent identity: BBa R0071
display id: BBa_R0071
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter (RhlR & C4-HSL regulated)
created: 2004-01-24T12:00:00Z
modified: 2015-05-08T01:14:15Z
mutable provenance: Pseudomonas aeruginosa rhlAB promoter
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Promoter activated by RhlR in concert with C4-HSL. C4-HSL is produced by RhlI, and acts as a quorum-sensing autoinducer. This is the natural sequence taken from Pseudomonas aeruginosa. Crosstalk: this promoter is also activated at a low level by LasR with its associated HSL.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: The -10 and -35 sites are labeled as identified in [Pearson97]. The part begins with the RhlR binding site, and (rather arbitrarily) ends with the first transcribed base (+1).
star: true
creator: Ronny Krashinsky
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: RhlR
sequence annotation: -35
sequence annotation: -10
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_R0071_sequence/1

RhlR
type: SequenceAnnotation
persistent identity: annotation297102
display id: annotation297102
version: 1
top level: RhlR+C4
location: https://synbiohub.org/public/igem/BBa_R0071/annotation297102/range297102/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0071/annotation297102/range297102/1
type: Range
persistent identity: range297102
display id: range297102
version: 1
top level: RhlR+C4
direction: direction/forward
start: 1
end: 20
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation297103
display id: annotation297103
version: 1
top level: RhlR+C4
location: https://synbiohub.org/public/igem/BBa_R0071/annotation297103/range297103/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0071/annotation297103/range297103/1
type: Range
persistent identity: range297103
display id: range297103
version: 1
top level: RhlR+C4
direction: direction/forward
start: 19
end: 24
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation297104
display id: annotation297104
version: 1
top level: RhlR+C4
location: https://synbiohub.org/public/igem/BBa_R0071/annotation297104/range297104/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0071/annotation297104/range297104/1
type: Range
persistent identity: range297104
display id: range297104
version: 1
top level: RhlR+C4
direction: direction/forward
start: 42
end: 47
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation297105
display id: annotation297105
version: 1
top level: RhlR+C4
location: https://synbiohub.org/public/igem/BBa_R0071/annotation297105/range297105/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_R0071/annotation297105/range297105/1
type: Range
persistent identity: range297105
display id: range297105
version: 1
top level: RhlR+C4
direction: direction/forward
start: 53
end: 53
orientation: inline

luxr
type: ComponentDefinition
persistent identity: BBa C0062
display id: BBa_C0062
version: 1
was generated by: iGEM to SBOL conversion
description: luxR repressor/activator, (no LVA?)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: V. fischeri <genbank>AF170104</genbank>
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: In complex with HSL, LuxR binds to the Lux promoter, activating transcription from Pr <bb_part>BBa_R0062</bb_part>, and repressing transcription from Pl <bb_part>BBa_R0063</bb_part>. The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the Lux activator, LuxR complexed to HSL. Two molecules of LuxR protein form a complex with two molecules the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription.
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: 2 silent point mutants were introduced in the coding sequence to remove internal XbaI and PstI sites. Mutation sites were chosen to replace codons commonly used in E. coli with codons used at a similar frequency.
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: luxR
sequence annotation: prefix
sequence annotation: BBa_C0062
sequence annotation: T
sequence annotation: A
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0062_sequence/1

luxR
type: SequenceAnnotation
persistent identity: annotation1766
display id: annotation1766
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation1766/range1766/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_C0062/annotation1766/range1766/1
type: Range
persistent identity: range1766
display id: range1766
version: 1
top level: luxr
direction: direction/forward
start: 1
end: 750
orientation: inline

prefix
type: SequenceAnnotation
persistent identity: annotation1762
display id: annotation1762
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation1762/range1762/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_C0062/annotation1762/range1762/1
type: Range
persistent identity: range1762
display id: range1762
version: 1
top level: luxr
direction: direction/forward
start: 1
end: 2
orientation: inline

BBa_C0062
type: SequenceAnnotation
persistent identity: annotation7039
display id: annotation7039
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation7039/range7039/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_C0062/annotation7039/range7039/1
type: Range
persistent identity: range7039
display id: range7039
version: 1
top level: luxr
direction: direction/forward
start: 1
end: 756
orientation: inline

T
type: SequenceAnnotation
persistent identity: annotation1764
display id: annotation1764
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation1764/range1764/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_C0062/annotation1764/range1764/1
type: Range
persistent identity: range1764
display id: range1764
version: 1
top level: luxr
direction: direction/forward
start: 174
end: 174
orientation: inline

A
type: SequenceAnnotation
persistent identity: annotation1765
display id: annotation1765
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation1765/range1765/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_C0062/annotation1765/range1765/1
type: Range
persistent identity: range1765
display id: range1765
version: 1
top level: luxr
direction: direction/forward
start: 492
end: 492
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213986
display id: annotation2213986
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation2213986/range2213986/1
role: feature/barcode
role: SO:0000807

https://synbiohub.org/public/igem/BBa_C0062/annotation2213986/range2213986/1
type: Range
persistent identity: range2213986
display id: range2213986
version: 1
top level: luxr
direction: direction/forward
start: 757
end: 781
orientation: inline

lux pR
type: ComponentDefinition
persistent identity: BBa R0062
display id: BBa_R0062
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter (luxR & HSL regulated -- lux pR)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:15Z
mutable provenance: V. fischeri
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Promoter activated by LuxR in concert with HSL The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the LuxR activator protein complexed with the autoinducer, 3-oxo-hexanoyl-HSL. Two molecules of LuxR protein form a complex with two molecules of the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription.
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: This promoter is based on the Vibrio fischeri quorum sensing gene promoters. Two genes LuxI and LuxR and transcribed in opposite directions as shown below. The original sequence from which the parts <bb_part>BBa_R0062</bb_part> and <bb_part>BBa_R0063</bb_part> were derived is shown in the picture below. <img src="<bb_file>Image1.gif</bb_file>" width="614" height="362">
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: BBa_R0062
sequence annotation: LuxR/HSL
sequence annotation: -35
sequence annotation: -10
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_R0062_sequence/1

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation7070
display id: annotation7070
version: 1
top level: lux pR
location: https://synbiohub.org/public/igem/BBa_R0062/annotation7070/range7070/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0062/annotation7070/range7070/1
type: Range
persistent identity: range7070
display id: range7070
version: 1
top level: lux pR
direction: direction/forward
start: 1
end: 55
orientation: inline

LuxR/HSL
type: SequenceAnnotation
persistent identity: annotation2045
display id: annotation2045
version: 1
top level: lux pR
location: https://synbiohub.org/public/igem/BBa_R0062/annotation2045/range2045/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0062/annotation2045/range2045/1
type: Range
persistent identity: range2045
display id: range2045
version: 1
top level: lux pR
direction: direction/forward
start: 1
end: 20
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2046
display id: annotation2046
version: 1
top level: lux pR
location: https://synbiohub.org/public/igem/BBa_R0062/annotation2046/range2046/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0062/annotation2046/range2046/1
type: Range
persistent identity: range2046
display id: range2046
version: 1
top level: lux pR
direction: direction/forward
start: 20
end: 25
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2047
display id: annotation2047
version: 1
top level: lux pR
location: https://synbiohub.org/public/igem/BBa_R0062/annotation2047/range2047/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0062/annotation2047/range2047/1
type: Range
persistent identity: range2047
display id: range2047
version: 1
top level: lux pR
direction: direction/forward
start: 42
end: 47
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2048
display id: annotation2048
version: 1
top level: lux pR
location: https://synbiohub.org/public/igem/BBa_R0062/annotation2048/range2048/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_R0062/annotation2048/range2048/1
type: Range
persistent identity: range2048
display id: range2048
version: 1
top level: lux pR
direction: direction/forward
start: 53
end: 53
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

cinr
type: ComponentDefinition
persistent identity: BBa C0077
display id: BBa_C0077
version: 1
was generated by: iGEM to SBOL conversion
description: cinR activator from Rhizobium leguminosarum (+LVA)
created: 2004-01-27T12:00:00Z
modified: 2015-08-31T04:07:24Z
mutable provenance: Rhizobium leguminosarum
owned by: myers
owned by: james
part status: Released HQ 2013
mutable description: In complex with O3-C14:1-HSL (made by BBa_C0076), CinR binds to the Cin promoter (BBa_R0077) and activates transcription.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: The regulatory locus cinRI in Rhizobium leguminosarum conrols a network of quorum-sensing loci Lithgow, JK; Wilkinson, A; Hardman, A; Rodelas, B; Wisniewski-Dye, F; Williams, P; Downie, AJ MOL. MICROBIOLOGY 37(1): 81-97, 2000 Change log: original STOP: tag -> taATAA
star: true
creator: crackdots
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: cinR
sequence annotation: LVA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0077_sequence/1

cinR
type: SequenceAnnotation
persistent identity: annotation301112
display id: annotation301112
version: 1
top level: cinr
location: https://synbiohub.org/public/igem/BBa_C0077/annotation301112/range301112/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_C0077/annotation301112/range301112/1
type: Range
persistent identity: range301112
display id: range301112
version: 1
top level: cinr
direction: direction/forward
start: 1
end: 723
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation306600
display id: annotation306600
version: 1
top level: cinr
location: https://synbiohub.org/public/igem/BBa_C0077/annotation306600/range306600/1
role: SO:0000324
role: feature/tag

https://synbiohub.org/public/igem/BBa_C0077/annotation306600/range306600/1
type: Range
persistent identity: range306600
display id: range306600
version: 1
top level: cinr
direction: direction/forward
start: 724
end: 756
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214000
display id: annotation2214000
version: 1
top level: cinr
location: https://synbiohub.org/public/igem/BBa_C0077/annotation2214000/range2214000/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_C0077/annotation2214000/range2214000/1
type: Range
persistent identity: range2214000
display id: range2214000
version: 1
top level: cinr
direction: direction/forward
start: 763
end: 787
orientation: inline

ECFP
type: ComponentDefinition
persistent identity: BBa E0022
display id: BBa_E0022
version: 1
was generated by: iGEM to SBOL conversion
description: enhanced cyan fluorescent protein derived from A. victoria GFP
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:25Z
mutable provenance: Modified from <bb_part>BBa_E0021</bb_part>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Cyan fluorescent protein (ECFP) reporter coding sequence without the Ribosome Binding Site. Modified with an LVA tail for rapid degradation of the protein and faster fall time for the emission.
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: BBa_E0022 cyan fluorescent protein is based on BioBrick part BBa_E0021. It has been modified to include a rapid degradation LVA tail, and includes the BioBrick standard assembly head and tail restriction sites. The RBS has been removed. The stop codon has been changed from TAA to a double stop codon TAATAA.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
type: DnaRegion
role: SO:0000804
role: partType/Reporter
sequence annotation: BBa_E0022
sequence annotation: CFP (LVA)
sequence annotation: A
sequence annotation: SsrA
sequence annotation: 2
sequence: https://synbiohub.org/public/igem/BBa_E0022_sequence/1

BBa_E0022
type: SequenceAnnotation
persistent identity: annotation7040
display id: annotation7040
version: 1
top level: ECFP
location: https://synbiohub.org/public/igem/BBa_E0022/annotation7040/range7040/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_E0022/annotation7040/range7040/1
type: Range
persistent identity: range7040
display id: range7040
version: 1
top level: ECFP
direction: direction/forward
start: 1
end: 762
orientation: inline

CFP (LVA)
type: SequenceAnnotation
persistent identity: annotation2150
display id: annotation2150
version: 1
top level: ECFP
location: https://synbiohub.org/public/igem/BBa_E0022/annotation2150/range2150/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_E0022/annotation2150/range2150/1
type: Range
persistent identity: range2150
display id: range2150
version: 1
top level: ECFP
direction: direction/forward
start: 1
end: 762
orientation: inline

A
type: SequenceAnnotation
persistent identity: annotation2155
display id: annotation2155
version: 1
top level: ECFP
location: https://synbiohub.org/public/igem/BBa_E0022/annotation2155/range2155/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_E0022/annotation2155/range2155/1
type: Range
persistent identity: range2155
display id: range2155
version: 1
top level: ECFP
direction: direction/forward
start: 69
end: 69
orientation: inline

SsrA
type: SequenceAnnotation
persistent identity: annotation2153
display id: annotation2153
version: 1
top level: ECFP
location: https://synbiohub.org/public/igem/BBa_E0022/annotation2153/range2153/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_E0022/annotation2153/range2153/1
type: Range
persistent identity: range2153
display id: range2153
version: 1
top level: ECFP
direction: direction/forward
start: 719
end: 756
orientation: inline

2
type: SequenceAnnotation
persistent identity: annotation2154
display id: annotation2154
version: 1
top level: ECFP
location: https://synbiohub.org/public/igem/BBa_E0022/annotation2154/range2154/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_E0022/annotation2154/range2154/1
type: Range
persistent identity: range2154
display id: range2154
version: 1
top level: ECFP
direction: direction/forward
start: 757
end: 762
orientation: inline

YFP
type: ComponentDefinition
persistent identity: BBa E0032
display id: BBa_E0032
version: 1
was generated by: iGEM to SBOL conversion
description: enhanced yellow fluorescent protein derived from A. victoria GFP
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:25Z
mutable provenance: Modified from <bb_part>BBa_E0031</bb_part>
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Yellow fluorescent protein (EYFP) reporter coding sequence without the Ribosome Binding Site. Modified with an LVA tail for rapid degradation of the protein and faster fall time for the emission. Annotated mutation cause a Q81L mutation that appears to not be near the active site.
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: BBa_E0032 yellow fluorescent protein is based on BioBrick part BBa_E0031. It has been modified to include a rapid degradation LVA tail, and includes the BioBrick standard assembly head and tail restriction sites. The RBS has been removed. The stop codon has been changed from TAA to a double stop codon TAATAA.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
type: DnaRegion
role: SO:0000804
role: partType/Reporter
sequence annotation: YFP (LVA)
sequence annotation: BBa_E0032
sequence annotation: A (Q->L)
sequence annotation: SsrA
sequence annotation: 2
sequence: https://synbiohub.org/public/igem/BBa_E0032_sequence/1

YFP (LVA)
type: SequenceAnnotation
persistent identity: annotation2156
display id: annotation2156
version: 1
top level: YFP
location: https://synbiohub.org/public/igem/BBa_E0032/annotation2156/range2156/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_E0032/annotation2156/range2156/1
type: Range
persistent identity: range2156
display id: range2156
version: 1
top level: YFP
direction: direction/forward
start: 1
end: 762
orientation: inline

BBa_E0032
type: SequenceAnnotation
persistent identity: annotation7041
display id: annotation7041
version: 1
top level: YFP
location: https://synbiohub.org/public/igem/BBa_E0032/annotation7041/range7041/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_E0032/annotation7041/range7041/1
type: Range
persistent identity: range7041
display id: range7041
version: 1
top level: YFP
direction: direction/forward
start: 1
end: 762
orientation: inline

A (Q->L)
type: SequenceAnnotation
persistent identity: annotation2161
display id: annotation2161
version: 1
top level: YFP
location: https://synbiohub.org/public/igem/BBa_E0032/annotation2161/range2161/1
role: feature/mutation
role: SO:0001059

https://synbiohub.org/public/igem/BBa_E0032/annotation2161/range2161/1
type: Range
persistent identity: range2161
display id: range2161
version: 1
top level: YFP
direction: direction/forward
start: 242
end: 242
orientation: inline

SsrA
type: SequenceAnnotation
persistent identity: annotation2160
display id: annotation2160
version: 1
top level: YFP
location: https://synbiohub.org/public/igem/BBa_E0032/annotation2160/range2160/1
role: SO:0000324
role: feature/tag

https://synbiohub.org/public/igem/BBa_E0032/annotation2160/range2160/1
type: Range
persistent identity: range2160
display id: range2160
version: 1
top level: YFP
direction: direction/forward
start: 718
end: 756
orientation: inline

2
type: SequenceAnnotation
persistent identity: annotation2159
display id: annotation2159
version: 1
top level: YFP
location: https://synbiohub.org/public/igem/BBa_E0032/annotation2159/range2159/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_E0032/annotation2159/range2159/1
type: Range
persistent identity: range2159
display id: range2159
version: 1
top level: YFP
direction: direction/forward
start: 757
end: 762
orientation: inline

mCherry
type: ComponentDefinition
persistent identity: BBa J06505
display id: BBa_J06505
version: 1
was generated by: iGEM to SBOL conversion
description: monomeric RFP optimized for bacteria
created: 2005-07-17T11:00:00Z
modified: 2015-08-31T04:08:18Z
mutable provenance: mCherry from Roger Y. Tsien's lab, altered to be BioBrick compatible and with an LVA tag added.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: mRFP (DsRed) derived, altered to be a biobrick by removing a PstI site and adding in the ends.
discontinued: false
dominant: false
experience: experience/None
group u list: _20_
m user id: 0
owner id: 340
owning group id: 20
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Made a point mutation to eliminate a PstI site in the middle and then added BioBrick ends with the LVA tag using PCR. Sequenced using primers that bind to the pSB1A2 plasmid on either side of the brick.
star: false
creator: ytwang
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: mCherry
sequence annotation: C->T (removing PstI site)
sequence annotation: LVA
sequence: https://synbiohub.org/public/igem/BBa_J06505_sequence/1

mCherry
type: SequenceAnnotation
persistent identity: annotation1585879
display id: annotation1585879
version: 1
top level: mCherry
location: https://synbiohub.org/public/igem/BBa_J06505/annotation1585879/range1585879/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_J06505/annotation1585879/range1585879/1
type: Range
persistent identity: range1585879
display id: range1585879
version: 1
top level: mCherry
direction: direction/forward
start: 1
end: 708
orientation: inline

C->T (removing PstI site)
type: SequenceAnnotation
persistent identity: annotation1585880
display id: annotation1585880
version: 1
top level: mCherry
location: https://synbiohub.org/public/igem/BBa_J06505/annotation1585880/range1585880/1
role: feature/mutation
role: SO:0001059

https://synbiohub.org/public/igem/BBa_J06505/annotation1585880/range1585880/1
type: Range
persistent identity: range1585880
display id: range1585880
version: 1
top level: mCherry
direction: direction/forward
start: 352
end: 352
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation1585887
display id: annotation1585887
version: 1
top level: mCherry
location: https://synbiohub.org/public/igem/BBa_J06505/annotation1585887/range1585887/1
role: SO:0000324
role: feature/tag

https://synbiohub.org/public/igem/BBa_J06505/annotation1585887/range1585887/1
type: Range
persistent identity: range1585887
display id: range1585887
version: 1
top level: mCherry
direction: direction/forward
start: 709
end: 717
orientation: inline

rhlR
type: ComponentDefinition
persistent identity: BBa C0071
display id: BBa_C0071
version: 1
was generated by: iGEM to SBOL conversion
description: rhlR repressor/activator from P. aeruginosa PA3477 (+LVA)
created: 2004-01-26T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: P. aeruginosa PA3477
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcriptional regulator, in complex with N-butyryl-HSL, RhlR binds to the Rhl promoter
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: (1/28/04 Edit Jamboree) (0) No BB sites (1) ATG (2) TAATAA (3) Blast checks out (4) No codon changes (5) Maybe chassis compatability (check by experiment) (6) Codon usage not optimal (but looks OK) (7) ssrA tag added
star: true
creator: Debra Lin, Srini Devadas, David Gray, Ronny Krashinsky, and Chris Zheng Liu, Boopers
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: rhlR
sequence annotation: LVA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0071_sequence/1

rhlR
type: SequenceAnnotation
persistent identity: annotation301192
display id: annotation301192
version: 1
top level: rhlR
location: https://synbiohub.org/public/igem/BBa_C0071/annotation301192/range301192/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_C0071/annotation301192/range301192/1
type: Range
persistent identity: range301192
display id: range301192
version: 1
top level: rhlR
direction: direction/forward
start: 1
end: 723
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation306523
display id: annotation306523
version: 1
top level: rhlR
location: https://synbiohub.org/public/igem/BBa_C0071/annotation306523/range306523/1
role: SO:0000324
role: feature/tag

https://synbiohub.org/public/igem/BBa_C0071/annotation306523/range306523/1
type: Range
persistent identity: range306523
display id: range306523
version: 1
top level: rhlR
direction: direction/forward
start: 724
end: 756
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213995
display id: annotation2213995
version: 1
top level: rhlR
location: https://synbiohub.org/public/igem/BBa_C0071/annotation2213995/range2213995/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_C0071/annotation2213995/range2213995/1
type: Range
persistent identity: range2213995
display id: range2213995
version: 1
top level: rhlR
direction: direction/forward
start: 763
end: 787
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

https://synbiohub.org/public/igem/BBa_J32011_sequence/1
type: Sequence
persistent identity: BBa J32011 sequence
display id: BBa_J32011_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaatactagagaaagaggagaaatactagatgaggaatgacggaggctttttgctgtggtgggacggtttgcgtagcgagatgcagccgatccacgacagccagggcgtgttcgccgtcctggaaaaggaagtgcggcgcctgggcttcgattactacgcctatggcgtgcgccacacgattcccttcacccggccgaagaccgaggtccatggcacctatcccaaggcctggctggagcgataccagatgcagaactacggggccgtggatccggcgatcctcaacggcctgcgctcctcggaaatggtggtctggagcgacagcctgttcgaccagagccggatgctctggaacgaggctcgcgattggggcctctgtgtcggcgcgaccttgccgatccgcgcgccgaacaatttgctcagcgtgctttccgtggcgcgcgaccagcagaacatctccagcttcgagcgcgaggaaatccgcctgcggctgcgttgcatgatcgagttgctgacccagaagctgaccgacctggagcatccgatgctgatgtccaacccggtctgcctgagccatcgcgaacgcgagatcctgcaatggaccgccgacggcaagagttccggggaaatcgccatcatcctgagcatctccgagagcacggtgaacttccaccacaagaacatccagaagaagttcgacgcgccgaacaagacgctggctgccgcctacgccgcggcgctgggtctcatcgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagaaagaggagaaatactagatggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaagttagtagcttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtcctgtgaaatctggcagttaccgttagctttcgaattggctaaaaagtgttctactagagaaagaggagaaatactagatgattgagaatacctatagcgaaaagttcgagtccgcgttcgaacagatcaaggcggcggccaacgtggatgccgccatccgtattctccaggcggaatataacctcgatttcgtcacctaccatctcgcccagacgatcgcgagcaagatcgattcg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0078_sequence/1
type: Sequence
persistent identity: BBa R0078 sequence
display id: BBa_R0078_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccctttgtgcgtccaaacggacgcacggcgctctaaagcgggtcgcgatctttcagattcgctcctcgcgctttcagtctttgttttggcgcatgtcgttatcgcaaaaccgctgcacacttttgcgcgacatgctctgatccccctcatctgggggggcctatctgagggaatttccgatccggctcgcctgaaccattctgctttccacgaacttgaaaacgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0071_sequence/1
type: Sequence
persistent identity: BBa R0071 sequence
display id: BBa_R0071_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcctgtgaaatctggcagttaccgttagctttcgaattggctaaaaagtgttc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0062_sequence/1
type: Sequence
persistent identity: BBa C0062 sequence
display id: BBa_C0062_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0062_sequence/1
type: Sequence
persistent identity: BBa R0062 sequence
display id: BBa_R0062_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0077_sequence/1
type: Sequence
persistent identity: BBa C0077 sequence
display id: BBa_C0077_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgattgagaatacctatagcgaaaagttcgagtccgcgttcgaacagatcaaggcggcggccaacgtggatgccgccatccgtattctccaggcggaatataacctcgatttcgtcacctaccatctcgcccagacgatcgcgagcaagatcgattcgcccttcgtgcgcaccacctatccggatgcctgggtttcccgctacctcctcaacagctatgtgaaggtcgatccgatcgtcaagcagggcttcgaacgccagctgcccttcgactggagcgaggtcgaaccgacgccggaggcctatgccatgctggtcgacgcccagaaacacggcatcggtggcaatggctactccatccccgtcgccgacaaggcgcagcgccgcgccctgctgtcgctgaatgcccgtataccggccgacgaatggaccgagctcgtgcgccgctgccgcaacgagtggatcgagatcgcccatctgatccaccgcaaggccgtctatgagctgcatggcgaaaacgatccggtgccggcattgtcgccgcgcgagatcgagtgtctgcactggaccgccctcggcaaggattacaaggatatttcggtcatcctgggcatatcagagcataccacacgcgattacctgaagaccgcccgcttcaagctcggctgcgccacgatctcggccgccgcgtcgcgggctgttcaattgcgcatcatcaatcccgctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0022_sequence/1
type: Sequence
persistent identity: BBa E0022 sequence
display id: BBa_E0022_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtgaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctggggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacatcagccacaacgtctatatcaccgccgacaagcagaagaacggcatcaaggccaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagaggcctgctgcaaacgacgaaaactacgctttagtagcttaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0032_sequence/1
type: Sequence
persistent identity: BBa E0032 sequence
display id: BBa_E0032_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagaggcctgctgcaaacgacgaaaactacgctttagtagcttaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J06505_sequence/1
type: Sequence
persistent identity: BBa J06505 sequence
display id: BBa_J06505_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaagttagtagcttaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0071_sequence/1
type: Sequence
persistent identity: BBa C0071 sequence
display id: BBa_C0071_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaggaatgacggaggctttttgctgtggtgggacggtttgcgtagcgagatgcagccgatccacgacagccagggcgtgttcgccgtcctggaaaaggaagtgcggcgcctgggcttcgattactacgcctatggcgtgcgccacacgattcccttcacccggccgaagaccgaggtccatggcacctatcccaaggcctggctggagcgataccagatgcagaactacggggccgtggatccggcgatcctcaacggcctgcgctcctcggaaatggtggtctggagcgacagcctgttcgaccagagccggatgctctggaacgaggctcgcgattggggcctctgtgtcggcgcgaccttgccgatccgcgcgccgaacaatttgctcagcgtgctttccgtggcgcgcgaccagcagaacatctccagcttcgagcgcgaggaaatccgcctgcggctgcgttgcatgatcgagttgctgacccagaagctgaccgacctggagcatccgatgctgatgtccaacccggtctgcctgagccatcgcgaacgcgagatcctgcaatggaccgccgacggcaagagttccggggaaatcgccatcatcctgagcatctccgagagcacggtgaacttccaccacaagaacatccagaagaagttcgacgcgccgaacaagacgctggctgccgcctacgccgcggcgctgggtctcatcgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z