LOCUS BBa_J32012 5748 bp DNA linear 21-Mar-2025 DEFINITION X-Verter Sender ACCESSION BBa_J32012 VERSION BBa_J32012.1 FEATURES Location/Qualifiers misc_feature 1..20 /label=LuxR/HSL promoter 20..25 /label=-35 promoter 42..47 /label=-10 misc_feature 53..53 /label=start misc_feature 1..55 /label=BBa_R0062 promoter 1..55 /label=BBa_R0062 misc_feature 68..71 /label=conserved RBS 64..75 /label=BBa_B0034 CDS 85..792 /label=cI lambda misc_feature 793..825 /label=LVA misc_feature 832..856 /label=Help:Barcodes CDS 82..856 /label=BBa_C0051 misc_feature 869..872 /label=conserved RBS 865..876 /label=BBa_B0034 misc_feature 885..903 /label=TetR 1 misc_feature 885..938 /label=BBa_R0040 promoter 904..909 /label=-35 misc_feature 910..928 /label=TetR 2 promoter 927..932 /label=-10 promoter 885..938 /label=BBa_R0040 CDS 945..1547 /label=rhlL misc_feature 1548..1580 /label=LVA misc_feature 1587..1611 /label=Help:Barcodes CDS 945..1611 /label=BBa_C0070 stem_loop 1631..1674 /label=stem_loop misc_feature 1620..1699 /label=BBa_B0010 terminator 1620..1699 /label=BBa_B0010 stem_loop 1715..1734 /label=T7 TE misc_feature 1741..1741 /label=stop polyA_site 1735..1748 /label=polya misc_feature 1708..1748 /label=BBa_B0012 terminator 1708..1748 /label=BBa_B0012 terminator 1620..1748 /label=BBa_B0015 misc_feature 1757..1776 /label=RhlR promoter 1775..1780 /label=-35 promoter 1798..1803 /label=-10 misc_feature 1809..1809 /label=start promoter 1757..1809 /label=BBa_R0071 misc_feature 1822..1825 /label=conserved RBS 1818..1829 /label=BBa_B0034 CDS 1839..2455 /label=tetR misc_feature 2456..2489 /label=SsrA misc_feature 2496..2520 /label=Help:Barcodes CDS 1836..2520 /label=BBa_C0040 misc_feature 2533..2536 /label=conserved RBS 2529..2540 /label=BBa_B0034 misc_feature 2551..2567 /label=lac O1 promoter 2568..2573 /label=-35 misc_feature 2574..2590 /label=lac O1 promoter 2591..2596 /label=-10 misc_feature 2549..2602 /label=BBa_R0011 promoter 2549..2602 /label=BBa_R0011 CDS 2610..3272 /label=CinI misc_feature 3273..3305 /label=LVA misc_feature 3312..3336 /label=Help:Barcodes CDS 2610..3336 /label=BBa_C0076 stem_loop 3356..3399 /label=stem_loop misc_feature 3345..3424 /label=BBa_B0010 terminator 3345..3424 /label=BBa_B0010 stem_loop 3440..3459 /label=T7 TE misc_feature 3466..3466 /label=stop polyA_site 3460..3473 /label=polya misc_feature 3433..3473 /label=BBa_B0012 terminator 3433..3473 /label=BBa_B0012 terminator 3345..3473 /label=BBa_B0015 misc_feature 3482..3701 /label=unidentified/uncharacterized CinR dependent stem_loop 3516..3555 /label=stem_loop stem_loop 3572..3617 /label=stem_loop promoter 3482..3706 /label=BBa_R0078 misc_feature 3719..3722 /label=conserved RBS 3715..3726 /label=BBa_B0034 misc_feature 4822..4860 /label=LVA CDS 3733..4860 /label=lacI-LVA misc_feature 3733..4860 /label=BBa_C0012 misc_feature 4861..4885 /label=Help:Barcodes CDS 3733..4860 /label=BBa_C0012 misc_feature 4898..4901 /label=conserved RBS 4894..4905 /label=BBa_B0034 promoter 4951..4956 /label=-10 promoter 4928..4933 /label=-35 misc_feature 4938..4954 /label=OR1 misc_feature 4914..4930 /label=OR2 misc_feature 4914..4962 /label=BBa_R0051 promoter 4914..4962 /label=BBa_R0051 misc_feature 5548..5579 /label=LVA CDS 4969..5547 /label=luxI misc_feature 4969..5586 /label=BBa_C0061 misc_feature 5587..5611 /label=Help:Barcodes CDS 4969..5586 /label=BBa_C0061 stem_loop 5631..5674 /label=stem_loop misc_feature 5620..5699 /label=BBa_B0010 terminator 5620..5699 /label=BBa_B0010 stem_loop 5715..5734 /label=T7 TE misc_feature 5741..5741 /label=stop polyA_site 5735..5748 /label=polya misc_feature 5708..5748 /label=BBa_B0012 terminator 5708..5748 /label=BBa_B0012 terminator 5620..5748 /label=BBa_B0015 ORIGIN 1 acctgtagga tcgtacaggt ttacgcaaga aaatggtttg ttatagtcga ataaatacta 61 gagaaagagg agaaatacta gatgagcaca aaaaagaaac cattaacaca agagcagctt 121 gaggacgcac gtcgccttaa agcaatttat gaaaaaaaga aaaatgaact tggcttatcc 181 caggaatctg tcgcagacaa gatggggatg gggcagtcag gcgttggtgc tttatttaat 241 ggcatcaatg cattaaatgc ttataacgcc gcattgcttg caaaaattct caaagttagc 301 gttgaagaat ttagcccttc aatcgccaga gaaatctacg agatgtatga agcggttagt 361 atgcagccgt cacttagaag tgagtatgag taccctgttt tttctcatgt tcaggcaggg 421 atgttctcac ctgagcttag aacctttacc aaaggtgatg cggagagatg ggtaagcaca 481 accaaaaaag ccagtgattc tgcattctgg cttgaggttg aaggtaattc catgaccgca 541 ccaacaggct ccaagccaag ctttcctgac ggaatgttaa ttctcgttga ccctgagcag 601 gctgttgagc caggtgattt ctgcatagcc agacttgggg gtgatgagtt taccttcaag 661 aaactgatca gggatagcgg tcaggtgttt ttacaaccac taaacccaca gtacccaatg 721 atcccatgca atgagagttg ttccgttgtg gggaaagtta tcgctagtca gtggcctgaa 781 gagacgtttg gcgctgcaaa cgacgaaaac tacgctttag tagcttaata acgctgatag 841 tgctagtgta gatcgctact agagaaagag gagaaatact agagtcccta tcagtgatag 901 agattgacat ccctatcagt gatagagata ctgagcacta ctagatgatc gaactgctgt 961 ccgaatccct ggaaggtctg tccgctgcta tgatcgctga actgggtcgt taccgtcacc 1021 aggttttcat cgaaaaactg ggttgggacg ttgtttccac ctcccgtgtt cgtgaccagg 1081 agttcgacca gttcgaccac ccgcagaccc gttacatcgt tgctatgtcc cgtcagggta 1141 tctgcggttg cgctcgtctg ctgccgacca ccgacgctta cctgctgaaa gacgttttcg 1201 cttacctgtg ctccgaaacc ccgccgtccg acccgtccgt ttgggaactg tcccgttacg 1261 ctgcttccgc tgctgacgac ccgcagctgg ctatgaaaat cttctggtcc tccctccagt 1321 gcgcttggta cctgggtgct tcctccgttg ttgctgttac caccaccgct atggaacgtt 1381 acttcgttcg taacggtgtt atcctccagc gtctgggtcc gccgcagaaa gttaaaggtg 1441 aaaccctggt tgctatctcc ttcccggctt accaggaacg tggtctggaa atgctgctgc 1501 gttaccaccc ggaatggctc cagggtgttc cgctgtccat ggctgttgct gcaaacgacg 1561 aaaactacgc tttagtagct taataactct gatagtgcta gtgtagatct ctactagagc 1621 caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg 1681 tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg 1741 cgtttatata ctagagtcct gtgaaatctg gcagttaccg ttagctttcg aattggctaa 1801 aaagtgttct actagagaaa gaggagaaat actagatgtc cagattagat aaaagtaaag 1861 tgattaacag cgcattagag ctgcttaatg aggtcggaat cgaaggttta acaacccgta 1921 aactcgccca gaagctaggt gtagagcagc ctacattgta ttggcatgta aaaaataagc 1981 gggctttgct cgacgcctta gccattgaga tgttagatag gcaccatact cacttttgcc 2041 ctttagaagg ggaaagctgg caagattttt tacgtaataa cgctaaaagt tttagatgtg 2101 ctttactaag tcatcgcgat ggagcaaaag tacatttagg tacacggcct acagaaaaac 2161 agtatgaaac tctcgaaaat caattagcct ttttatgcca acaaggtttt tcactagaga 2221 atgcattata tgcactcagc gctgtggggc attttacttt aggttgcgta ttggaagatc 2281 aagagcatca agtcgctaaa gaagaaaggg aaacacctac tactgatagt atgccgccat 2341 tattacgaca agctatcgaa ttatttgatc accaaggtgc agagccagcc ttcttattcg 2401 gccttgaatt gatcatatgc ggattagaaa aacaacttaa atgtgaaagt gggtccgctg 2461 caaacgacga aaactacgct ttagtagctt aataacactg atagtgctag tgtagatcac 2521 tactagagaa agaggagaaa tactagagaa ttgtgagcgg ataacaattg acattgtgag 2581 cggataacaa gatactgagc acatactaga tgttcgttat catccaggct cacgaatacc 2641 agaaatacgc tgctgttctg gaccagatgt tccgtctgcg taaaaaagtt ttcgctgaca 2701 ccctgtgctg ggacgttccg gttatcggtc cgtacgaacg tgactcctac gactccctgg 2761 ctccggctta cctggtttgg tgcaacgact cccgtacccg tctgtacggt ggtatgcgtc 2821 tgatgccgac caccggtccg accctgctgt acgacgtttt ccgtgaaacc ttcccggacg 2881 ctgctgacct gatcgctccg ggtatctggg aaggtacccg tatgtgcatc gacgaagaag 2941 ctatcgctaa agacttcccg gaaatcgacg ctggtcgtgc tttctccatg atgctgctgg 3001 ctctgtgcga atgcgctctg gaccacggta tccacaccat gatctccaac tacgaaccgt 3061 acctgaaacg tgtttacaaa cgtgctggtg ctgaagttga agaactgggt cgtgctgacg 3121 gttacggtaa atacccggtt tgctgcggtg ctttcgaagt ttccgaccgt gttctgcgta 3181 aaatgcgtgc tgctctgggt ctgaccctgc cgctgtacgt tcgtcacgtt ccggctcgtt 3241 ccgttgttac ccagttcctg gaaatggctg ctgctgctaa cgacgaaaac tacgctctgg 3301 ttgcttaata accctgatag tgctagtgta gatccctact agagccaggc atcaaataaa 3361 acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc 3421 tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt atatactaga 3481 gccctttgtg cgtccaaacg gacgcacggc gctctaaagc gggtcgcgat ctttcagatt 3541 cgctcctcgc gctttcagtc tttgttttgg cgcatgtcgt tatcgcaaaa ccgctgcaca 3601 cttttgcgcg acatgctctg atccccctca tctggggggg cctatctgag ggaatttccg 3661 atccggctcg cctgaaccat tctgctttcc acgaacttga aaacgctact agagaaagag 3721 gagaaatact agatggtgaa tgtgaaacca gtaacgttat acgatgtcgc agagtatgcc 3781 ggtgtctctt atcagaccgt ttcccgcgtg gtgaaccagg ccagccacgt ttctgcgaaa 3841 acgcgggaaa aagtggaagc ggcgatggcg gagctgaatt acattcccaa ccgcgtggca 3901 caacaactgg cgggcaaaca gtcgttgctg attggcgttg ccacctccag tctggccctg 3961 cacgcgccgt cgcaaattgt cgcggcgatt aaatctcgcg ccgatcaact gggtgccagc 4021 gtggtggtgt cgatggtaga acgaagcggc gtcgaagcct gtaaagcggc ggtgcacaat 4081 cttctcgcgc aacgcgtcag tgggctgatc attaactatc cgctggatga ccaggatgcc 4141 attgctgtgg aagctgcctg cactaatgtt ccggcgttat ttcttgatgt ctctgaccag 4201 acacccatca acagtattat tttctcccat gaagacggta cgcgactggg cgtggagcat 4261 ctggtcgcat tgggtcacca gcaaatcgcg ctgttagcgg gcccattaag ttctgtctcg 4321 gcgcgtctgc gtctggctgg ctggcataaa tatctcactc gcaatcaaat tcagccgata 4381 gcggaacggg aaggcgactg gagtgccatg tccggttttc aacaaaccat gcaaatgctg 4441 aatgagggca tcgttcccac tgcgatgctg gttgccaacg atcagatggc gctgggcgca 4501 atgcgcgcca ttaccgagtc cgggctgcgc gttggtgcgg atatctcggt agtgggatac 4561 gacgataccg aagacagctc atgttatatc ccgccgttaa ccaccatcaa acaggatttt 4621 cgcctgctgg ggcaaaccag cgtggaccgc ttgctgcaac tctctcaggg ccaggcggtg 4681 aagggcaatc agctgttgcc cgtctcactg gtgaaaagaa aaaccaccct ggcgcccaat 4741 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt 4801 tcccgactgg aaagcgggca ggctgcaaac gacgaaaact acgctttagt agcttaataa 4861 ctctgatagt gctagtgtag atctctacta gagaaagagg agaaatacta gagtaacacc 4921 gtgcgtgttg actattttac ctctggcggt gataatggtt gctactagat gactataatg 4981 ataaaaaaat cggatttttt ggcaattcca tcggaggagt ataaaggtat tctaagtctt 5041 cgttatcaag tgtttaagca aagacttgag tgggacttag ttgtagaaaa taaccttgaa 5101 tcagatgagt atgataactc aaatgcagaa tatatttatg cttgtgatga tactgaaaat 5161 gtaagtggat gctggcgttt attacctaca acaggtgatt atatgctgaa aagtgttttt 5221 cctgaattgc ttggtcaaca gagtgctccc aaagatccta atatagtcga attaagtcgt 5281 tttgctgtag gtaaaaatag ctcaaagata aataactctg ctagtgaaat tacaatgaaa 5341 ctatttgaag ctatatataa acacgctgtt agtcaaggta ttacagaata tgtaacagta 5401 acatcaacag caatagagcg atttttaaag cgtattaaag ttccttgtca tcgtattgga 5461 gacaaagaaa ttcatgtatt aggtgatact aaatcggttg tattgtctat gcctattaat 5521 gaacagttta aaaaagcagt cttaaatgct gcaaacgacg aaaactacgc tttagtagct 5581 taataactct gatagtgcta gtgtagatct ctactagagc caggcatcaa ataaaacgaa 5641 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct 5701 actagagtca cactggctca ccttcgggtg ggcctttctg cgtttata //