##gff-version 3
##sequence-region BBa_J32012 1 5748
BBa_J32012	.	CDS	945	1611	.	+	0	ID=BBa_C0070;Name=BBa_C0070
BBa_J32012	.	engineered_tag	1587	1611	.	+	0	ID=annotation2213996;Name=Help:Barcodes;Parent=BBa_C0070
BBa_J32012	.	tag	1548	1580	.	+	0	ID=annotation306820;Name=LVA;Parent=BBa_C0070
BBa_J32012	.	CDS	945	1547	.	+	0	ID=annotation301203;Name=rhlL;Parent=BBa_C0070
BBa_J32012	.	promoter	2549	2602	.	+	0	ID=BBa_R0011;Name=BBa_R0011
BBa_J32012	.	promoter	2568	2573	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_J32012	.	promoter	2591	2596	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_J32012	.	operator	2574	2590	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_J32012	.	engineered_region	2549	2602	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_J32012	.	operator	2551	2567	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_J32012	.	terminator	5620	5748	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_J32012	.	terminator	5708	5748	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_J32012	.	stem_loop	5715	5734	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J32012	.	stop_codon	5741	5741	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J32012	.	engineered_region	5708	5748	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J32012	.	polyA_site	5735	5748	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J32012	.	terminator	5620	5699	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_J32012	.	stem_loop	5631	5674	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J32012	.	engineered_region	5620	5699	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J32012	.	terminator	3345	3473	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_J32012	.	terminator	3433	3473	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_J32012	.	stem_loop	3440	3459	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J32012	.	stop_codon	3466	3466	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J32012	.	engineered_region	3433	3473	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J32012	.	polyA_site	3460	3473	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J32012	.	terminator	3345	3424	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_J32012	.	stem_loop	3356	3399	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J32012	.	engineered_region	3345	3424	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J32012	.	promoter	885	938	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_J32012	.	promoter	927	932	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_J32012	.	promoter	904	909	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_J32012	.	non_covalent_binding_site	910	928	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_J32012	.	engineered_region	885	938	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_J32012	.	non_covalent_binding_site	885	903	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_J32012	.	ribosome_entry_site	1818	1829	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_J32012	.	conserved_region	1822	1825	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J32012	.	CDS	3733	4860	.	+	0	ID=BBa_C0012;Name=BBa_C0012
BBa_J32012	.	engineered_tag	4861	4885	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_J32012	.	CDS	3733	4860	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
BBa_J32012	.	engineered_region	3733	4860	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
BBa_J32012	.	tag	4822	4860	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
BBa_J32012	.	terminator	1620	1748	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_J32012	.	terminator	1708	1748	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_J32012	.	stem_loop	1715	1734	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J32012	.	stop_codon	1741	1741	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J32012	.	engineered_region	1708	1748	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J32012	.	polyA_site	1735	1748	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J32012	.	terminator	1620	1699	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_J32012	.	stem_loop	1631	1674	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J32012	.	engineered_region	1620	1699	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J32012	.	CDS	1836	2520	.	+	0	ID=BBa_C0040;Name=BBa_C0040
BBa_J32012	.	engineered_tag	2496	2520	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_J32012	.	tag	2456	2489	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_J32012	.	CDS	1839	2455	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_J32012	.	promoter	1	55	.	+	0	ID=BBa_R0062;Name=BBa_R0062
BBa_J32012	.	promoter	20	25	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_J32012	.	promoter	42	47	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_J32012	.	start_codon	53	53	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_J32012	.	engineered_region	1	55	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_J32012	.	non_covalent_binding_site	1	20	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_J32012	.	promoter	1757	1809	.	+	0	ID=BBa_R0071;Name=BBa_R0071
BBa_J32012	.	start_codon	1809	1809	.	+	0	ID=annotation297105;Name=start;Parent=BBa_R0071
BBa_J32012	.	non_covalent_binding_site	1757	1776	.	+	0	ID=annotation297102;Name=RhlR;Parent=BBa_R0071
BBa_J32012	.	promoter	1798	1803	.	+	0	ID=annotation297104;Name=-10;Parent=BBa_R0071
BBa_J32012	.	promoter	1775	1780	.	+	0	ID=annotation297103;Name=-35;Parent=BBa_R0071
BBa_J32012	.	ribosome_entry_site	2529	2540	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_J32012	.	conserved_region	2533	2536	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J32012	.	CDS	4969	5586	.	+	0	ID=BBa_C0061;Name=BBa_C0061
BBa_J32012	.	CDS	4969	5547	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_J32012	.	engineered_region	4969	5586	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_J32012	.	tag	5548	5579	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_J32012	.	engineered_tag	5587	5611	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_J32012	.	ribosome_entry_site	3715	3726	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_J32012	.	conserved_region	3719	3722	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J32012	.	ribosome_entry_site	865	876	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_J32012	.	conserved_region	869	872	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J32012	.	ribosome_entry_site	4894	4905	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_J32012	.	conserved_region	4898	4901	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J32012	.	CDS	2610	3336	.	+	0	ID=BBa_C0076;Name=BBa_C0076
BBa_J32012	.	engineered_tag	3312	3336	.	+	0	ID=annotation2214001;Name=Help:Barcodes;Parent=BBa_C0076
BBa_J32012	.	tag	3273	3305	.	+	0	ID=annotation306586;Name=LVA;Parent=BBa_C0076
BBa_J32012	.	CDS	2610	3272	.	+	0	ID=annotation301035;Name=CinI;Parent=BBa_C0076
BBa_J32012	.	ribosome_entry_site	64	75	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_J32012	.	conserved_region	68	71	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J32012	.	promoter	3482	3706	.	+	0	ID=BBa_R0078;Name=BBa_R0078
BBa_J32012	.	sequence_feature	3482	3701	.	+	0	ID=annotation318234;Name=unidentified/uncharacterized CinR dependent;Parent=BBa_R0078
BBa_J32012	.	stem_loop	3516	3555	.	+	0	ID=annotation318244;Name=stem_loop;Parent=BBa_R0078
BBa_J32012	.	stem_loop	3572	3617	.	+	0	ID=annotation318250;Name=stem_loop;Parent=BBa_R0078
BBa_J32012	.	CDS	82	856	.	+	0	ID=BBa_C0051;Name=BBa_C0051
BBa_J32012	.	tag	793	825	.	+	0	ID=annotation23335;Name=LVA;Parent=BBa_C0051
BBa_J32012	.	CDS	85	792	.	+	0	ID=annotation23334;Name=cI lambda;Parent=BBa_C0051
BBa_J32012	.	engineered_tag	832	856	.	+	0	ID=annotation2213991;Name=Help:Barcodes;Parent=BBa_C0051
BBa_J32012	.	promoter	4914	4962	.	+	0	ID=BBa_R0051;Name=BBa_R0051
BBa_J32012	.	engineered_region	4914	4962	.	+	0	ID=annotation7067;Name=BBa_R0051;Parent=BBa_R0051
BBa_J32012	.	promoter	4951	4956	.	+	0	ID=annotation2022;Name=-10;Parent=BBa_R0051
BBa_J32012	.	promoter	4928	4933	.	+	0	ID=annotation2023;Name=-35;Parent=BBa_R0051
BBa_J32012	.	non_covalent_binding_site	4938	4954	.	+	0	ID=annotation2024;Name=OR1;Parent=BBa_R0051
BBa_J32012	.	non_covalent_binding_site	4914	4930	.	+	0	ID=annotation2025;Name=OR2;Parent=BBa_R0051
>BBa_J32012
acctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaatactagagaaagaggagaaatact
agatgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaa
gaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttattt
aatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcc
cttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccc
tgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggta
agcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctcca
agccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccag
acttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacag
tacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttg
gcgctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgctactagagaaaga
ggagaaatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagatga
tcgaactgctgtccgaatccctggaaggtctgtccgctgctatgatcgctgaactgggtcgttaccgtcaccaggtttt
catcgaaaaactgggttgggacgttgtttccacctcccgtgttcgtgaccaggagttcgaccagttcgaccacccgcag
acccgttacatcgttgctatgtcccgtcagggtatctgcggttgcgctcgtctgctgccgaccaccgacgcttacctgc
tgaaagacgttttcgcttacctgtgctccgaaaccccgccgtccgacccgtccgtttgggaactgtcccgttacgctgc
ttccgctgctgacgacccgcagctggctatgaaaatcttctggtcctccctccagtgcgcttggtacctgggtgcttcc
tccgttgttgctgttaccaccaccgctatggaacgttacttcgttcgtaacggtgttatcctccagcgtctgggtccgc
cgcagaaagttaaaggtgaaaccctggttgctatctccttcccggcttaccaggaacgtggtctggaaatgctgctgcg
ttaccacccggaatggctccagggtgttccgctgtccatggctgttgctgcaaacgacgaaaactacgctttagtagct
taataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactg
ggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttc
tgcgtttatatactagagtcctgtgaaatctggcagttaccgttagctttcgaattggctaaaaagtgttctactagag
aaagaggagaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcg
gaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaa
taagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaa
agctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtac
atttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttc
actagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaa
gtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatc
accaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaag
tgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagag
aaagaggagaaatactagagaattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatact
agatgttcgttatcatccaggctcacgaataccagaaatacgctgctgttctggaccagatgttccgtctgcgtaaaaa
agttttcgctgacaccctgtgctgggacgttccggttatcggtccgtacgaacgtgactcctacgactccctggctccg
gcttacctggtttggtgcaacgactcccgtacccgtctgtacggtggtatgcgtctgatgccgaccaccggtccgaccc
tgctgtacgacgttttccgtgaaaccttcccggacgctgctgacctgatcgctccgggtatctgggaaggtacccgtat
gtgcatcgacgaagaagctatcgctaaagacttcccggaaatcgacgctggtcgtgctttctccatgatgctgctggct
ctgtgcgaatgcgctctggaccacggtatccacaccatgatctccaactacgaaccgtacctgaaacgtgtttacaaac
gtgctggtgctgaagttgaagaactgggtcgtgctgacggttacggtaaatacccggtttgctgcggtgctttcgaagt
ttccgaccgtgttctgcgtaaaatgcgtgctgctctgggtctgaccctgccgctgtacgttcgtcacgttccggctcgt
tccgttgttacccagttcctggaaatggctgctgctgctaacgacgaaaactacgctctggttgcttaataaccctgat
agtgctagtgtagatccctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgtttt
atctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatac
tagagccctttgtgcgtccaaacggacgcacggcgctctaaagcgggtcgcgatctttcagattcgctcctcgcgcttt
cagtctttgttttggcgcatgtcgttatcgcaaaaccgctgcacacttttgcgcgacatgctctgatccccctcatctg
ggggggcctatctgagggaatttccgatccggctcgcctgaaccattctgctttccacgaacttgaaaacgctactaga
gaaagaggagaaatactagatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttat
cagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcgg
agctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccag
tctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtg
tcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctga
tcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttga
tgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtc
gcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggc
ataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaaca
aaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatg
cgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcat
gttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaact
ctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaat
acgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggc
aggctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagaaaga
ggagaaatactagagtaacaccgtgcgtgttgactattttacctctggcggtgataatggttgctactagatgactata
atgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatcaagtgttta
agcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatat
ttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagt
gtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggta
aaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttag
tcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcat
cgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatgaacagttta
aaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatc
tctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcgg
tgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata