LOCUS BBa_J32027 3612 bp DNA linear 22-Nov-2024 DEFINITION Prey II ACCESSION BBa_J32027 VERSION BBa_J32027.1 FEATURES Location/Qualifiers misc_feature 3..19 /label=lac O1 promoter 20..25 /label=-35 misc_feature 26..42 /label=lac O1 promoter 43..48 /label=-10 misc_feature 1..54 /label=BBa_R0011 promoter 1..54 /label=BBa_R0011 misc_feature 68..71 /label=conserved RBS 64..75 /label=BBa_B0034 misc_feature 661..692 /label=LVA CDS 82..660 /label=luxI misc_feature 82..699 /label=BBa_C0061 misc_feature 700..724 /label=Help:Barcodes CDS 82..699 /label=BBa_C0061 stem_loop 744..787 /label=stem_loop misc_feature 733..812 /label=BBa_B0010 terminator 733..812 /label=BBa_B0010 stem_loop 828..847 /label=T7 TE misc_feature 854..854 /label=stop polyA_site 848..861 /label=polya misc_feature 821..861 /label=BBa_B0012 terminator 821..861 /label=BBa_B0012 terminator 733..861 /label=BBa_B0015 misc_feature 872..888 /label=lac O1 promoter 889..894 /label=-35 misc_feature 895..911 /label=lac O1 promoter 912..917 /label=-10 misc_feature 870..923 /label=BBa_R0011 promoter 870..923 /label=BBa_R0011 misc_feature 937..940 /label=conserved RBS 933..944 /label=BBa_B0034 CDS 951..1670 /label=GFP protein CDS 951..1670 /label=BBa_E0040 misc_feature 1683..1686 /label=conserved RBS 1679..1690 /label=BBa_B0034 CDS 1697..2425 /label=BBa_C0177 stem_loop 2445..2488 /label=stem_loop misc_feature 2434..2513 /label=BBa_B0010 terminator 2434..2513 /label=BBa_B0010 stem_loop 2529..2548 /label=T7 TE misc_feature 2555..2555 /label=stop polyA_site 2549..2562 /label=polya misc_feature 2522..2562 /label=BBa_B0012 terminator 2522..2562 /label=BBa_B0012 terminator 2434..2562 /label=BBa_B0015 misc_feature 2571..2790 /label=unidentified/uncharacterized CinR dependent stem_loop 2605..2644 /label=stem_loop stem_loop 2661..2706 /label=stem_loop promoter 2571..2795 /label=BBa_R0078 misc_feature 2808..2811 /label=conserved RBS 2804..2815 /label=BBa_B0034 CDS 2822..3475 /label=BBa_J32026 stem_loop 3495..3538 /label=stem_loop misc_feature 3484..3563 /label=BBa_B0010 terminator 3484..3563 /label=BBa_B0010 stem_loop 3579..3598 /label=T7 TE misc_feature 3605..3605 /label=stop polyA_site 3599..3612 /label=polya misc_feature 3572..3612 /label=BBa_B0012 terminator 3572..3612 /label=BBa_B0012 terminator 3484..3612 /label=BBa_B0015 ORIGIN 1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta 61 gagaaagagg agaaatacta gatgactata atgataaaaa aatcggattt tttggcaatt 121 ccatcggagg agtataaagg tattctaagt cttcgttatc aagtgtttaa gcaaagactt 181 gagtgggact tagttgtaga aaataacctt gaatcagatg agtatgataa ctcaaatgca 241 gaatatattt atgcttgtga tgatactgaa aatgtaagtg gatgctggcg tttattacct 301 acaacaggtg attatatgct gaaaagtgtt tttcctgaat tgcttggtca acagagtgct 361 cccaaagatc ctaatatagt cgaattaagt cgttttgctg taggtaaaaa tagctcaaag 421 ataaataact ctgctagtga aattacaatg aaactatttg aagctatata taaacacgct 481 gttagtcaag gtattacaga atatgtaaca gtaacatcaa cagcaataga gcgattttta 541 aagcgtatta aagttccttg tcatcgtatt ggagacaaag aaattcatgt attaggtgat 601 actaaatcgg ttgtattgtc tatgcctatt aatgaacagt ttaaaaaagc agtcttaaat 661 gctgcaaacg acgaaaacta cgctttagta gcttaataac tctgatagtg ctagtgtaga 721 tctctactag agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc 781 gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg 841 gtgggccttt ctgcgtttat atactagaga attgtgagcg gataacaatt gacattgtga 901 gcggataaca agatactgag cacatactag agaaagagga gaaatactag atgcgtaaag 961 gagaagaact tttcactgga gttgtcccaa ttcttgttga attagatggt gatgttaatg 1021 ggcacaaatt ttctgtcagt ggagagggtg aaggtgatgc aacatacgga aaacttaccc 1081 ttaaatttat ttgcactact ggaaaactac ctgttccatg gccaacactt gtcactactt 1141 tcggttatgg tgttcaatgc tttgcgagat acccagatca tatgaaacag catgactttt 1201 tcaagagtgc catgcccgaa ggttatgtac aggaaagaac tatatttttc aaagatgacg 1261 ggaactacaa gacacgtgct gaagtcaagt ttgaaggtga tacccttgtt aatagaatcg 1321 agttaaaagg tattgatttt aaagaagatg gaaacattct tggacacaaa ttggaataca 1381 actataactc acacaatgta tacatcatgg cagacaaaca aaagaatgga atcaaagtta 1441 acttcaaaat tagacacaac attgaagatg gaagcgttca actagcagac cattatcaac 1501 aaaatactcc aattggcgat ggccctgtcc ttttaccaga caaccattac ctgtccacac 1561 aatctgccct ttcgaaagat cccaacgaaa agagagacca catggtcctt cttgagtttg 1621 taacagctgc tgggattaca catggcatgg atgaactata caaataataa tactagagaa 1681 agaggagaaa tactagatga ttgagaatac ctatagcgaa aagttcgagt ccgcgttcga 1741 acagatcaag gcggcggcca acgtggatgc cgccatccgt attctccagg cggaatataa 1801 cctcgatttc gtcacctacc atctcgccca gacgatcgcg agcaagatcg attcgccctt 1861 cgtgcgcacc acctatccgg atgcctgggt ttcccgctac ctcctcaaca gctatgtgaa 1921 ggtcgatccg atcgtcaagc agggcttcga acgccagctg cccttcgact ggagcgaggt 1981 cgaaccgacg ccggaggcct atgccatgct ggtcgacgcc cagaaacacg gcatcggtgg 2041 caatggctac tccatccccg tcgccgacaa ggcgcagcgc cgcgccctgc tgtcgctgaa 2101 tgcccgtata ccggccgacg aatggaccga gctcgtgcgc cgctgccgca acgagtggat 2161 cgagatcgcc catctgatcc accgcaaggc cgtctatgag ctgcatggcg aaaacgatcc 2221 ggtgccggca ttgtcgccgc gcgagatcga gtgtctgcac tggaccgccc tcggcaagga 2281 ttacaaggat atttcggtca tcctgggcat atcagagcat accacacgcg attacctgaa 2341 gaccgcccgc ttcaagctcg gctgcgccac gatctcggcc gccgcgtcgc gggctgttca 2401 attgcgcatc atcaatccct aataatacta gagccaggca tcaaataaaa cgaaaggctc 2461 agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga 2521 gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta tatactagag ccctttgtgc 2581 gtccaaacgg acgcacggcg ctctaaagcg ggtcgcgatc tttcagattc gctcctcgcg 2641 ctttcagtct ttgttttggc gcatgtcgtt atcgcaaaac cgctgcacac ttttgcgcga 2701 catgctctga tccccctcat ctgggggggc ctatctgagg gaatttccga tccggctcgc 2761 ctgaaccatt ctgctttcca cgaacttgaa aacgctacta gagaaagagg agaaatacta 2821 gatgaccatg attacgccaa gctatttagg tgacactata gaatactcaa gctatgcatc 2881 aagcttggta ccgagctcgg atccgctagt aacggccgcc agtgtgctgg agttctgtag 2941 atatccatca cactggagac cgctcgagca tgcatccaga gggcccaatt cgccctatag 3001 tgagtcgtat tacaattcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg 3061 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga 3121 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctatacgtac ggcagtttaa 3181 ggtttacacc tataaaagag agagccgtta tcgtctgttt gtggatgtac agagtgatat 3241 tattgacacg ccggggcgac ggatggtgat ccccctggcc agtgcacgtc tgctgtcaga 3301 taaagtctcc cgtgaacttt acccggtggt gcatatcggg gatgaaagct ggcgcatgat 3361 gaccaccgat atggccagtg tgccggtctc cgttatcggg gaagaagtgg ctgatctcag 3421 ccaccgcgaa aatgacatca aaaacgccat taacctgatg ttctggggaa tataatacta 3481 gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct 3541 gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt 3601 tctgcgttta ta //