##gff-version 3
##sequence-region BBa_J32027 1 3612
BBa_J32027	.	CDS	951	1670	.	+	0	ID=BBa_E0040;Name=BBa_E0040
BBa_J32027	.	CDS	951	1670	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_J32027	.	promoter	2571	2795	.	+	0	ID=BBa_R0078;Name=BBa_R0078
BBa_J32027	.	sequence_feature	2571	2790	.	+	0	ID=annotation318234;Name=unidentified/uncharacterized CinR dependent;Parent=BBa_R0078
BBa_J32027	.	stem_loop	2661	2706	.	+	0	ID=annotation318250;Name=stem_loop;Parent=BBa_R0078
BBa_J32027	.	stem_loop	2605	2644	.	+	0	ID=annotation318244;Name=stem_loop;Parent=BBa_R0078
BBa_J32027	.	promoter	1	54	.	+	0	ID=BBa_R0011;Name=BBa_R0011
BBa_J32027	.	operator	26	42	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_J32027	.	engineered_region	1	54	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_J32027	.	operator	3	19	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_J32027	.	promoter	20	25	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_J32027	.	promoter	43	48	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_J32027	.	terminator	2434	2562	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_J32027	.	terminator	2434	2513	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_J32027	.	engineered_region	2434	2513	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J32027	.	stem_loop	2445	2488	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J32027	.	terminator	2522	2562	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_J32027	.	engineered_region	2522	2562	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J32027	.	stem_loop	2529	2548	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J32027	.	polyA_site	2549	2562	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J32027	.	stop_codon	2555	2555	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J32027	.	CDS	82	699	.	+	0	ID=BBa_C0061;Name=BBa_C0061
BBa_J32027	.	CDS	82	660	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_J32027	.	tag	661	692	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_J32027	.	engineered_tag	700	724	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_J32027	.	engineered_region	82	699	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_J32027	.	ribosome_entry_site	933	944	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_J32027	.	conserved_region	937	940	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J32027	.	ribosome_entry_site	2804	2815	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_J32027	.	conserved_region	2808	2811	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J32027	.	CDS	2822	3475	.	+	0	ID=BBa_J32026;Name=BBa_J32026
BBa_J32027	.	terminator	733	861	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_J32027	.	terminator	733	812	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_J32027	.	engineered_region	733	812	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J32027	.	stem_loop	744	787	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J32027	.	terminator	821	861	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_J32027	.	engineered_region	821	861	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J32027	.	stem_loop	828	847	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J32027	.	polyA_site	848	861	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J32027	.	stop_codon	854	854	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J32027	.	ribosome_entry_site	1679	1690	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_J32027	.	conserved_region	1683	1686	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J32027	.	ribosome_entry_site	64	75	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_J32027	.	conserved_region	68	71	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J32027	.	terminator	3484	3612	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_J32027	.	terminator	3484	3563	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_J32027	.	engineered_region	3484	3563	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J32027	.	stem_loop	3495	3538	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J32027	.	terminator	3572	3612	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_J32027	.	engineered_region	3572	3612	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J32027	.	stem_loop	3579	3598	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J32027	.	polyA_site	3599	3612	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J32027	.	stop_codon	3605	3605	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J32027	.	CDS	1697	2425	.	+	0	ID=BBa_C0177;Name=BBa_C0177
BBa_J32027	.	promoter	870	923	.	+	0	ID=BBa_R0011;Name=BBa_R0011
BBa_J32027	.	operator	895	911	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_J32027	.	engineered_region	870	923	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_J32027	.	operator	872	888	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_J32027	.	promoter	889	894	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_J32027	.	promoter	912	917	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
>BBa_J32027
aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagaaagaggagaaatact
agatgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgtta
tcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaat
gcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattata
tgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttt
tgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaa
cacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaag
ttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaa
tgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgc
tagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctg
ttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagag
aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagaaagaggagaaatact
agatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaa
attttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaa
ctacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatga
aacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaa
ctacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaa
gaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaa
agaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaaca
aaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagat
cccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactat
acaaataataatactagagaaagaggagaaatactagatgattgagaatacctatagcgaaaagttcgagtccgcgttc
gaacagatcaaggcggcggccaacgtggatgccgccatccgtattctccaggcggaatataacctcgatttcgtcacct
accatctcgcccagacgatcgcgagcaagatcgattcgcccttcgtgcgcaccacctatccggatgcctgggtttcccg
ctacctcctcaacagctatgtgaaggtcgatccgatcgtcaagcagggcttcgaacgccagctgcccttcgactggagc
gaggtcgaaccgacgccggaggcctatgccatgctggtcgacgcccagaaacacggcatcggtggcaatggctactcca
tccccgtcgccgacaaggcgcagcgccgcgccctgctgtcgctgaatgcccgtataccggccgacgaatggaccgagct
cgtgcgccgctgccgcaacgagtggatcgagatcgcccatctgatccaccgcaaggccgtctatgagctgcatggcgaa
aacgatccggtgccggcattgtcgccgcgcgagatcgagtgtctgcactggaccgccctcggcaaggattacaaggata
tttcggtcatcctgggcatatcagagcataccacacgcgattacctgaagaccgcccgcttcaagctcggctgcgccac
gatctcggccgccgcgtcgcgggctgttcaattgcgcatcatcaatccctaataatactagagccaggcatcaaataaa
acgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacact
ggctcaccttcgggtgggcctttctgcgtttatatactagagccctttgtgcgtccaaacggacgcacggcgctctaaa
gcgggtcgcgatctttcagattcgctcctcgcgctttcagtctttgttttggcgcatgtcgttatcgcaaaaccgctgc
acacttttgcgcgacatgctctgatccccctcatctgggggggcctatctgagggaatttccgatccggctcgcctgaa
ccattctgctttccacgaacttgaaaacgctactagagaaagaggagaaatactagatgaccatgattacgccaagcta
tttaggtgacactatagaatactcaagctatgcatcaagcttggtaccgagctcggatccgctagtaacggccgccagt
gtgctggagttctgtagatatccatcacactggagaccgctcgagcatgcatccagagggcccaattcgccctatagtg
agtcgtattacaattcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgcct
tgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagc
ctatacgtacggcagtttaaggtttacacctataaaagagagagccgttatcgtctgtttgtggatgtacagagtgata
ttattgacacgccggggcgacggatggtgatccccctggccagtgcacgtctgctgtcagataaagtctcccgtgaact
ttacccggtggtgcatatcggggatgaaagctggcgcatgatgaccaccgatatggccagtgtgccggtctccgttatc
ggggaagaagtggctgatctcagccaccgcgaaaatgacatcaaaaacgccattaacctgatgttctggggaatataat
actagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtga
acgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata