BBa_J32027
type: ComponentDefinition
persistent identity: BBa J32027
display id: BBa_J32027
version: 1
was generated by: iGEM to SBOL conversion
description: Prey II
created: 2006-09-01T11:00:00Z
modified: 2015-08-31T04:08:46Z
mutable provenance: .
owned by: james
owned by: myers
mutable description: .
discontinued: false
dominant: false
group u list: _50_
m user id: 0
owner id: 495
owning group id: 50
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: .
star: false
creator: Austen Heinz
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_B0034
component: BBa_R0011
component: BBa_B0015
component: BBa_C0177
component: BBa_E0040
component: BBa_C0061
component: BBa_J32026
component: BBa_R0078
sequence annotation: BBa_R0011
sequence annotation: BBa_B0034
sequence annotation: BBa_C0061
sequence annotation: BBa_B0015
sequence annotation: BBa_E0040
sequence annotation: BBa_C0177
sequence annotation: BBa_R0078
sequence annotation: BBa_J32026
sequence: https://synbiohub.org/public/igem/BBa_J32027_sequence/1

BBa_B0034
type: Component
persistent identity: component2314817
display id: component2314817
version: 1
top level: BBa_J32027
definition: BBa_B0034
access: public

BBa_R0011
type: Component
persistent identity: component2314811
display id: component2314811
version: 1
top level: BBa_J32027
definition: lacI+pL
access: public

BBa_B0015
type: Component
persistent identity: component2314847
display id: component2314847
version: 1
top level: BBa_J32027
definition: BBa_B0015
access: public

BBa_C0177
type: Component
persistent identity: component2314840
display id: component2314840
version: 1
top level: BBa_J32027
definition: cinR
access: public

BBa_B0034
type: Component
persistent identity: component2314839
display id: component2314839
version: 1
top level: BBa_J32027
definition: BBa_B0034
access: public

BBa_E0040
type: Component
persistent identity: component2314837
display id: component2314837
version: 1
top level: BBa_J32027
definition: GFP
access: public

BBa_B0034
type: Component
persistent identity: component2314835
display id: component2314835
version: 1
top level: BBa_J32027
definition: BBa_B0034
access: public

BBa_R0011
type: Component
persistent identity: component2314829
display id: component2314829
version: 1
top level: BBa_J32027
definition: lacI+pL
access: public

BBa_B0015
type: Component
persistent identity: component2314828
display id: component2314828
version: 1
top level: BBa_J32027
definition: BBa_B0015
access: public

BBa_C0061
type: Component
persistent identity: component2314819
display id: component2314819
version: 1
top level: BBa_J32027
definition: luxI
access: public

BBa_B0015
type: Component
persistent identity: component2314861
display id: component2314861
version: 1
top level: BBa_J32027
definition: BBa_B0015
access: public

BBa_J32026
type: Component
persistent identity: component2314854
display id: component2314854
version: 1
top level: BBa_J32027
definition: LacZA-ccdB
access: public

BBa_B0034
type: Component
persistent identity: component2314853
display id: component2314853
version: 1
top level: BBa_J32027
definition: BBa_B0034
access: public

BBa_R0078
type: Component
persistent identity: component2314851
display id: component2314851
version: 1
top level: BBa_J32027
definition: cinR
access: public

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation2314811
display id: annotation2314811
version: 1
top level: BBa_J32027
location: https://synbiohub.org/public/igem/BBa_J32027/annotation2314811/range2314811/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_J32027/annotation2314811/range2314811/1
type: Range
persistent identity: range2314811
display id: range2314811
version: 1
top level: BBa_J32027
direction: direction/forward
start: 1
end: 54
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2314817
display id: annotation2314817
version: 1
top level: BBa_J32027
location: https://synbiohub.org/public/igem/BBa_J32027/annotation2314817/range2314817/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J32027/annotation2314817/range2314817/1
type: Range
persistent identity: range2314817
display id: range2314817
version: 1
top level: BBa_J32027
direction: direction/reverse
start: 64
end: 75
orientation: inline

BBa_C0061
type: SequenceAnnotation
persistent identity: annotation2314819
display id: annotation2314819
version: 1
top level: BBa_J32027
location: https://synbiohub.org/public/igem/BBa_J32027/annotation2314819/range2314819/1
component: BBa_C0061

https://synbiohub.org/public/igem/BBa_J32027/annotation2314819/range2314819/1
type: Range
persistent identity: range2314819
display id: range2314819
version: 1
top level: BBa_J32027
direction: direction/forward
start: 82
end: 699
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2314828
display id: annotation2314828
version: 1
top level: BBa_J32027
location: https://synbiohub.org/public/igem/BBa_J32027/annotation2314828/range2314828/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_J32027/annotation2314828/range2314828/1
type: Range
persistent identity: range2314828
display id: range2314828
version: 1
top level: BBa_J32027
direction: direction/reverse
start: 733
end: 861
orientation: inline

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation2314829
display id: annotation2314829
version: 1
top level: BBa_J32027
location: https://synbiohub.org/public/igem/BBa_J32027/annotation2314829/range2314829/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_J32027/annotation2314829/range2314829/1
type: Range
persistent identity: range2314829
display id: range2314829
version: 1
top level: BBa_J32027
direction: direction/forward
start: 870
end: 923
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2314835
display id: annotation2314835
version: 1
top level: BBa_J32027
location: https://synbiohub.org/public/igem/BBa_J32027/annotation2314835/range2314835/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J32027/annotation2314835/range2314835/1
type: Range
persistent identity: range2314835
display id: range2314835
version: 1
top level: BBa_J32027
direction: direction/reverse
start: 933
end: 944
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2314837
display id: annotation2314837
version: 1
top level: BBa_J32027
location: https://synbiohub.org/public/igem/BBa_J32027/annotation2314837/range2314837/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_J32027/annotation2314837/range2314837/1
type: Range
persistent identity: range2314837
display id: range2314837
version: 1
top level: BBa_J32027
direction: direction/reverse
start: 951
end: 1670
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2314839
display id: annotation2314839
version: 1
top level: BBa_J32027
location: https://synbiohub.org/public/igem/BBa_J32027/annotation2314839/range2314839/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J32027/annotation2314839/range2314839/1
type: Range
persistent identity: range2314839
display id: range2314839
version: 1
top level: BBa_J32027
direction: direction/reverse
start: 1679
end: 1690
orientation: inline

BBa_C0177
type: SequenceAnnotation
persistent identity: annotation2314840
display id: annotation2314840
version: 1
top level: BBa_J32027
location: https://synbiohub.org/public/igem/BBa_J32027/annotation2314840/range2314840/1
component: BBa_C0177

https://synbiohub.org/public/igem/BBa_J32027/annotation2314840/range2314840/1
type: Range
persistent identity: range2314840
display id: range2314840
version: 1
top level: BBa_J32027
direction: direction/reverse
start: 1697
end: 2425
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2314847
display id: annotation2314847
version: 1
top level: BBa_J32027
location: https://synbiohub.org/public/igem/BBa_J32027/annotation2314847/range2314847/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_J32027/annotation2314847/range2314847/1
type: Range
persistent identity: range2314847
display id: range2314847
version: 1
top level: BBa_J32027
direction: direction/reverse
start: 2434
end: 2562
orientation: inline

BBa_R0078
type: SequenceAnnotation
persistent identity: annotation2314851
display id: annotation2314851
version: 1
top level: BBa_J32027
location: https://synbiohub.org/public/igem/BBa_J32027/annotation2314851/range2314851/1
component: BBa_R0078

https://synbiohub.org/public/igem/BBa_J32027/annotation2314851/range2314851/1
type: Range
persistent identity: range2314851
display id: range2314851
version: 1
top level: BBa_J32027
direction: direction/reverse
start: 2571
end: 2795
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2314853
display id: annotation2314853
version: 1
top level: BBa_J32027
location: https://synbiohub.org/public/igem/BBa_J32027/annotation2314853/range2314853/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J32027/annotation2314853/range2314853/1
type: Range
persistent identity: range2314853
display id: range2314853
version: 1
top level: BBa_J32027
direction: direction/reverse
start: 2804
end: 2815
orientation: inline

BBa_J32026
type: SequenceAnnotation
persistent identity: annotation2314854
display id: annotation2314854
version: 1
top level: BBa_J32027
location: https://synbiohub.org/public/igem/BBa_J32027/annotation2314854/range2314854/1
component: BBa_J32026

https://synbiohub.org/public/igem/BBa_J32027/annotation2314854/range2314854/1
type: Range
persistent identity: range2314854
display id: range2314854
version: 1
top level: BBa_J32027
direction: direction/reverse
start: 2822
end: 3475
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2314861
display id: annotation2314861
version: 1
top level: BBa_J32027
location: https://synbiohub.org/public/igem/BBa_J32027/annotation2314861/range2314861/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_J32027/annotation2314861/range2314861/1
type: Range
persistent identity: range2314861
display id: range2314861
version: 1
top level: BBa_J32027
direction: direction/reverse
start: 3484
end: 3612
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

luxI
type: ComponentDefinition
persistent identity: BBa C0061
display id: BBa_C0061
version: 1
was generated by: iGEM to SBOL conversion
description: autoinducer synthetase for AHL
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: V. fischeri <genbank>AF170104</genbank>
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Synthesizes 3OC6HSL, which binds to LuxR. The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the Lux repressor, LuxR. Two molecules of LuxR protein form a complex with two molecules the signalling compound HSL. This complex binds to a palindromic site on the promoter, increasing the rate of transcription.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: An LVA tail (sequence: AANDENYALVA) was added to increase protein degradation. .
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: BBa_C0061
sequence annotation: luxI
sequence annotation: LVA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0061_sequence/1

BBa_C0061
type: SequenceAnnotation
persistent identity: annotation7038
display id: annotation7038
version: 1
top level: luxI
location: https://synbiohub.org/public/igem/BBa_C0061/annotation7038/range7038/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_C0061/annotation7038/range7038/1
type: Range
persistent identity: range7038
display id: range7038
version: 1
top level: luxI
direction: direction/forward
start: 1
end: 618
orientation: inline

luxI
type: SequenceAnnotation
persistent identity: annotation1761
display id: annotation1761
version: 1
top level: luxI
location: https://synbiohub.org/public/igem/BBa_C0061/annotation1761/range1761/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_C0061/annotation1761/range1761/1
type: Range
persistent identity: range1761
display id: range1761
version: 1
top level: luxI
direction: direction/forward
start: 1
end: 579
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation1760
display id: annotation1760
version: 1
top level: luxI
location: https://synbiohub.org/public/igem/BBa_C0061/annotation1760/range1760/1
role: SO:0000324
role: feature/tag

https://synbiohub.org/public/igem/BBa_C0061/annotation1760/range1760/1
type: Range
persistent identity: range1760
display id: range1760
version: 1
top level: luxI
direction: direction/forward
start: 580
end: 611
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213985
display id: annotation2213985
version: 1
top level: luxI
location: https://synbiohub.org/public/igem/BBa_C0061/annotation2213985/range2213985/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_C0061/annotation2213985/range2213985/1
type: Range
persistent identity: range2213985
display id: range2213985
version: 1
top level: luxI
direction: direction/forward
start: 619
end: 643
orientation: inline

GFP
type: ComponentDefinition
persistent identity: BBa E0040
display id: BBa_E0040
version: 1
was generated by: iGEM to SBOL conversion
description: green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212
created: 2004-09-29T11:00:00Z
modified: 2016-01-26T02:09:38Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: GFP (mut3b) [note that this part does not have a barcode]
discontinued: false
dominant: true
experience: experience/Works
group u list: _11_1_
m user id: 4206
owner id: 61
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: jcbraff
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: GFP protein
sequence: https://synbiohub.org/public/igem/BBa_E0040_sequence/1

GFP protein
type: SequenceAnnotation
persistent identity: annotation1934520
display id: annotation1934520
version: 1
top level: GFP
location: https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
type: Range
persistent identity: range1934520
display id: range1934520
version: 1
top level: GFP
direction: direction/forward
start: 1
end: 720
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

lacI+pL
type: ComponentDefinition
persistent identity: BBa R0011
display id: BBa_R0011
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter (lacI regulated, lambda pL hybrid)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: represillator of Elowitz and Leibler (2000)
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) The PLlac 0-1 promoter is a hybrid regulatory region consisting of the promoter P(L) of phage lambda with the cI binding sites replaced with lacO1. The hybrid design allows for strong promotion that can nevertheless be tightly repressed by LacI, the Lac inhibitor (i.e. repressor) (<bb_part>BBa_C0010</bb_part>) ([LUTZ97]). The activity of the promoter can be regulated over a >600-fold range by IPTG in E.Coli DH5-alpha-Z1 (same paper reference).
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: hybrid promoter design to create strong promoter that is, at the same time, highly repressible. note that the upstream operator installed in this hybrid is slightly different than the one in the original source (Lutz and Bujard, 1997). the most upstream operator region is slightly truncated in the represillator version, so that both operators in the hybrid are the same sequence. see references for details. also, the sequence has been truncated after the transcriptional start site. LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and increase transcription. This part is incompatible with environments containing lactose or lactose analogs.
star: true
creator: Neelaksh Varshney, Grace Kenney, Daniel Shen, Samantha Sutton
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0011
sequence annotation: lac O1
sequence annotation: -35
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0011_sequence/1

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation7064
display id: annotation7064
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation7064/range7064/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0011/annotation7064/range7064/1
type: Range
persistent identity: range7064
display id: range7064
version: 1
top level: lacI+pL
direction: direction/forward
start: 1
end: 54
orientation: inline

lac O1
type: SequenceAnnotation
persistent identity: annotation1999
display id: annotation1999
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation1999/range1999/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_R0011/annotation1999/range1999/1
type: Range
persistent identity: range1999
display id: range1999
version: 1
top level: lacI+pL
direction: direction/forward
start: 3
end: 19
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2000
display id: annotation2000
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation2000/range2000/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0011/annotation2000/range2000/1
type: Range
persistent identity: range2000
display id: range2000
version: 1
top level: lacI+pL
direction: direction/forward
start: 20
end: 25
orientation: inline

lac O1
type: SequenceAnnotation
persistent identity: annotation2001
display id: annotation2001
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation2001/range2001/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_R0011/annotation2001/range2001/1
type: Range
persistent identity: range2001
display id: range2001
version: 1
top level: lacI+pL
direction: direction/forward
start: 26
end: 42
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2002
display id: annotation2002
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation2002/range2002/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0011/annotation2002/range2002/1
type: Range
persistent identity: range2002
display id: range2002
version: 1
top level: lacI+pL
direction: direction/forward
start: 43
end: 48
orientation: inline

cinR
type: ComponentDefinition
persistent identity: BBa C0177
display id: BBa_C0177
version: 1
was generated by: iGEM to SBOL conversion
description: cinR (-LVA)
created: 2004-05-26T11:00:00Z
modified: 2015-08-31T04:07:24Z
owned by: james
owned by: myers
mutable description: same as C0077 except no LVA tag
discontinued: false
dominant: false
experience: experience/None
group u list: _11_1_
m user id: 0
owner id: 61
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: jcbraff
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence: https://synbiohub.org/public/igem/BBa_C0177_sequence/1

LacZA-ccdB
type: ComponentDefinition
persistent identity: BBa J32026
display id: BBa_J32026
version: 1
was generated by: iGEM to SBOL conversion
description: lacZalpha-ccdB
created: 2006-09-01T11:00:00Z
modified: 2015-08-31T04:08:46Z
mutable provenance: a
owned by: james
owned by: myers
mutable description: a
discontinued: true
dominant: false
experience: experience/None
group u list: _50_
m user id: 0
owner id: 495
owning group id: 50
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable notes: a
star: false
creator: Austen Heinz
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence: https://synbiohub.org/public/igem/BBa_J32026_sequence/1

cinR
type: ComponentDefinition
persistent identity: BBa R0078
display id: BBa_R0078
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter (cinR and HSL regulated)
created: 2004-01-29T12:00:00Z
modified: 2015-05-08T01:14:15Z
mutable provenance: Rhizobium leguminosarum
owned by: james
owned by: myers
part status: Released HQ 2013
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Drew Endy
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: unidentified/uncharacterized CinR dependent
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_R0078_sequence/1

unidentified/uncharacterized CinR dependent
type: SequenceAnnotation
persistent identity: annotation318234
display id: annotation318234
version: 1
top level: cinR
location: https://synbiohub.org/public/igem/BBa_R0078/annotation318234/range318234/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_R0078/annotation318234/range318234/1
type: Range
persistent identity: range318234
display id: range318234
version: 1
top level: cinR
direction: direction/forward
start: 1
end: 220
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation318244
display id: annotation318244
version: 1
top level: cinR
location: https://synbiohub.org/public/igem/BBa_R0078/annotation318244/range318244/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_R0078/annotation318244/range318244/1
type: Range
persistent identity: range318244
display id: range318244
version: 1
top level: cinR
direction: direction/forward
start: 35
end: 74
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation318250
display id: annotation318250
version: 1
top level: cinR
location: https://synbiohub.org/public/igem/BBa_R0078/annotation318250/range318250/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_R0078/annotation318250/range318250/1
type: Range
persistent identity: range318250
display id: range318250
version: 1
top level: cinR
direction: direction/forward
start: 91
end: 136
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

https://synbiohub.org/public/igem/BBa_J32027_sequence/1
type: Sequence
persistent identity: BBa J32027 sequence
display id: BBa_J32027_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagaaagaggagaaatactagatgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagaattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagaaagaggagaaatactagatgattgagaatacctatagcgaaaagttcgagtccgcgttcgaacagatcaaggcggcggccaacgtggatgccgccatccgtattctccaggcggaatataacctcgatttcgtcacctaccatctcgcccagacgatcgcgagcaagatcgattcgcccttcgtgcgcaccacctatccggatgcctgggtttcccgctacctcctcaacagctatgtgaaggtcgatccgatcgtcaagcagggcttcgaacgccagctgcccttcgactggagcgaggtcgaaccgacgccggaggcct…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0177_sequence/1
type: Sequence
persistent identity: BBa C0177 sequence
display id: BBa_C0177_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgattgagaatacctatagcgaaaagttcgagtccgcgttcgaacagatcaaggcggcggccaacgtggatgccgccatccgtattctccaggcggaatataacctcgatttcgtcacctaccatctcgcccagacgatcgcgagcaagatcgattcgcccttcgtgcgcaccacctatccggatgcctgggtttcccgctacctcctcaacagctatgtgaaggtcgatccgatcgtcaagcagggcttcgaacgccagctgcccttcgactggagcgaggtcgaaccgacgccggaggcctatgccatgctggtcgacgcccagaaacacggcatcggtggcaatggctactccatccccgtcgccgacaaggcgcagcgccgcgccctgctgtcgctgaatgcccgtataccggccgacgaatggaccgagctcgtgcgccgctgccgcaacgagtggatcgagatcgcccatctgatccaccgcaaggccgtctatgagctgcatggcgaaaacgatccggtgccggcattgtcgccgcgcgagatcgagtgtctgcactggaccgccctcggcaaggattacaaggatatttcggtcatcctgggcatatcagagcataccacacgcgattacctgaagaccgcccgcttcaagctcggctgcgccacgatctcggccgccgcgtcgcgggctgttcaattgcgcatcatcaatccctaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0061_sequence/1
type: Sequence
persistent identity: BBa C0061 sequence
display id: BBa_C0061_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0040_sequence/1
type: Sequence
persistent identity: BBa E0040 sequence
display id: BBa_E0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: myers
owned by: james
elements: atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0011_sequence/1
type: Sequence
persistent identity: BBa R0011 sequence
display id: BBa_R0011_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J32026_sequence/1
type: Sequence
persistent identity: BBa J32026 sequence
display id: BBa_J32026_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaccatgattacgccaagctatttaggtgacactatagaatactcaagctatgcatcaagcttggtaccgagctcggatccgctagtaacggccgccagtgtgctggagttctgtagatatccatcacactggagaccgctcgagcatgcatccagagggcccaattcgccctatagtgagtcgtattacaattcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctatacgtacggcagtttaaggtttacacctataaaagagagagccgttatcgtctgtttgtggatgtacagagtgatattattgacacgccggggcgacggatggtgatccccctggccagtgcacgtctgctgtcagataaagtctcccgtgaactttacccggtggtgcatatcggggatgaaagctggcgcatgatgaccaccgatatggccagtgtgccggtctccgttatcggggaagaagtggctgatctcagccaccgcgaaaatgacatcaaaaacgccattaacctgatgttctggggaatataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0078_sequence/1
type: Sequence
persistent identity: BBa R0078 sequence
display id: BBa_R0078_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccctttgtgcgtccaaacggacgcacggcgctctaaagcgggtcgcgatctttcagattcgctcctcgcgctttcagtctttgttttggcgcatgtcgttatcgcaaaaccgctgcacacttttgcgcgacatgctctgatccccctcatctgggggggcctatctgagggaatttccgatccggctcgcctgaaccattctgctttccacgaacttgaaaacgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z