BBa_J32029
type: ComponentDefinition
persistent identity: BBa J32029
display id: BBa_J32029
version: 1
was generated by: iGEM to SBOL conversion
description: Luminescent Reporter
created: 2006-10-31T12:00:00Z
modified: 2015-08-31T04:08:46Z
mutable provenance: a
owned by: james
owned by: myers
mutable description: _
discontinued: false
dominant: false
experience: experience/None
group u list: _50_
m user id: 0
owner id: 495
owning group id: 50
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: a
star: false
creator: Austen Heinz
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_R0011
component: BBa_J32007
component: BBa_B0034
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_R0011
sequence annotation: BBa_B0034
sequence annotation: BBa_J32007
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_J32029_sequence/1

BBa_R0011
type: Component
persistent identity: component1909484
display id: component1909484
version: 1
top level: BBa_J32029
definition: lacI+pL
access: public

BBa_J32007
type: Component
persistent identity: component1909498
display id: component1909498
version: 1
top level: BBa_J32029
definition: LuxCDABE
access: public

BBa_B0034
type: Component
persistent identity: component1909490
display id: component1909490
version: 1
top level: BBa_J32029
definition: BBa_B0034
access: public

BBa_B0012
type: Component
persistent identity: component1909501
display id: component1909501
version: 1
top level: BBa_J32029
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1909499
display id: component1909499
version: 1
top level: BBa_J32029
definition: BBa_B0010
access: public

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation1909484
display id: annotation1909484
version: 1
top level: BBa_J32029
location: https://synbiohub.org/public/igem/BBa_J32029/annotation1909484/range1909484/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_J32029/annotation1909484/range1909484/1
type: Range
persistent identity: range1909484
display id: range1909484
version: 1
top level: BBa_J32029
direction: direction/forward
start: 1
end: 54
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1909490
display id: annotation1909490
version: 1
top level: BBa_J32029
location: https://synbiohub.org/public/igem/BBa_J32029/annotation1909490/range1909490/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J32029/annotation1909490/range1909490/1
type: Range
persistent identity: range1909490
display id: range1909490
version: 1
top level: BBa_J32029
direction: direction/reverse
start: 64
end: 75
orientation: inline

BBa_J32007
type: SequenceAnnotation
persistent identity: annotation1909498
display id: annotation1909498
version: 1
top level: BBa_J32029
location: https://synbiohub.org/public/igem/BBa_J32029/annotation1909498/range1909498/1
component: BBa_J32007

https://synbiohub.org/public/igem/BBa_J32029/annotation1909498/range1909498/1
type: Range
persistent identity: range1909498
display id: range1909498
version: 1
top level: BBa_J32029
direction: direction/reverse
start: 82
end: 5879
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1909499
display id: annotation1909499
version: 1
top level: BBa_J32029
location: https://synbiohub.org/public/igem/BBa_J32029/annotation1909499/range1909499/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J32029/annotation1909499/range1909499/1
type: Range
persistent identity: range1909499
display id: range1909499
version: 1
top level: BBa_J32029
direction: direction/forward
start: 5888
end: 5967
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1909501
display id: annotation1909501
version: 1
top level: BBa_J32029
location: https://synbiohub.org/public/igem/BBa_J32029/annotation1909501/range1909501/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J32029/annotation1909501/range1909501/1
type: Range
persistent identity: range1909501
display id: range1909501
version: 1
top level: BBa_J32029
direction: direction/forward
start: 5976
end: 6016
orientation: inline

LuxCDABE
type: ComponentDefinition
persistent identity: BBa J32007
display id: BBa_J32007
version: 1
was generated by: iGEM to SBOL conversion
description: Photorhabdus luminescens LuxCDABE
created: 2006-07-13T11:00:00Z
modified: 2015-08-31T04:08:45Z
mutable provenance: Mike Winson NCBI Accession M90093
owned by: james
owned by: myers
mutable description: Photorhabdus luminescens fatty acid reductase (luxC), acyltransferase (luxD), luciferase (luxA), luciferase (luxB), and luxE (luxE) genes
discontinued: false
dominant: false
experience: experience/None
group u list: _50_
m user id: 0
owner id: 495
owning group id: 50
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: PstI site needs to be removed
star: true
creator: Austen Heinz, Pat O
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: LuxC
sequence annotation: Photorhabdus luminescens LuxCDABE
sequence annotation: J32007
sequence annotation: LuxD
sequence annotation: LuxA
sequence annotation: LuxB
sequence annotation: LuxE
sequence: https://synbiohub.org/public/igem/BBa_J32007_sequence/1

LuxC
type: SequenceAnnotation
persistent identity: annotation1885689
display id: annotation1885689
version: 1
top level: LuxCDABE
location: https://synbiohub.org/public/igem/BBa_J32007/annotation1885689/range1885689/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_J32007/annotation1885689/range1885689/1
type: Range
persistent identity: range1885689
display id: range1885689
version: 1
top level: LuxCDABE
direction: direction/forward
start: 1
end: 1444
orientation: inline

Photorhabdus luminescens LuxCDABE
type: SequenceAnnotation
persistent identity: annotation1885688
display id: annotation1885688
version: 1
top level: LuxCDABE
location: https://synbiohub.org/public/igem/BBa_J32007/annotation1885688/range1885688/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_J32007/annotation1885688/range1885688/1
type: Range
persistent identity: range1885688
display id: range1885688
version: 1
top level: LuxCDABE
direction: direction/forward
start: 1
end: 5798
orientation: inline

J32007
type: SequenceAnnotation
persistent identity: annotation1885687
display id: annotation1885687
version: 1
top level: LuxCDABE
location: https://synbiohub.org/public/igem/BBa_J32007/annotation1885687/range1885687/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_J32007/annotation1885687/range1885687/1
type: Range
persistent identity: range1885687
display id: range1885687
version: 1
top level: LuxCDABE
direction: direction/forward
start: 1
end: 5798
orientation: inline

LuxD
type: SequenceAnnotation
persistent identity: annotation1885690
display id: annotation1885690
version: 1
top level: LuxCDABE
location: https://synbiohub.org/public/igem/BBa_J32007/annotation1885690/range1885690/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_J32007/annotation1885690/range1885690/1
type: Range
persistent identity: range1885690
display id: range1885690
version: 1
top level: LuxCDABE
direction: direction/forward
start: 1455
end: 2378
orientation: inline

LuxA
type: SequenceAnnotation
persistent identity: annotation1885691
display id: annotation1885691
version: 1
top level: LuxCDABE
location: https://synbiohub.org/public/igem/BBa_J32007/annotation1885691/range1885691/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_J32007/annotation1885691/range1885691/1
type: Range
persistent identity: range1885691
display id: range1885691
version: 1
top level: LuxCDABE
direction: direction/forward
start: 2427
end: 3509
orientation: inline

LuxB
type: SequenceAnnotation
persistent identity: annotation1885692
display id: annotation1885692
version: 1
top level: LuxCDABE
location: https://synbiohub.org/public/igem/BBa_J32007/annotation1885692/range1885692/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_J32007/annotation1885692/range1885692/1
type: Range
persistent identity: range1885692
display id: range1885692
version: 1
top level: LuxCDABE
direction: direction/forward
start: 3524
end: 4507
orientation: inline

LuxE
type: SequenceAnnotation
persistent identity: annotation1885693
display id: annotation1885693
version: 1
top level: LuxCDABE
location: https://synbiohub.org/public/igem/BBa_J32007/annotation1885693/range1885693/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_J32007/annotation1885693/range1885693/1
type: Range
persistent identity: range1885693
display id: range1885693
version: 1
top level: LuxCDABE
direction: direction/forward
start: 4686
end: 5798
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

lacI+pL
type: ComponentDefinition
persistent identity: BBa R0011
display id: BBa_R0011
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter (lacI regulated, lambda pL hybrid)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: represillator of Elowitz and Leibler (2000)
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) The PLlac 0-1 promoter is a hybrid regulatory region consisting of the promoter P(L) of phage lambda with the cI binding sites replaced with lacO1. The hybrid design allows for strong promotion that can nevertheless be tightly repressed by LacI, the Lac inhibitor (i.e. repressor) (<bb_part>BBa_C0010</bb_part>) ([LUTZ97]). The activity of the promoter can be regulated over a >600-fold range by IPTG in E.Coli DH5-alpha-Z1 (same paper reference).
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: hybrid promoter design to create strong promoter that is, at the same time, highly repressible. note that the upstream operator installed in this hybrid is slightly different than the one in the original source (Lutz and Bujard, 1997). the most upstream operator region is slightly truncated in the represillator version, so that both operators in the hybrid are the same sequence. see references for details. also, the sequence has been truncated after the transcriptional start site. LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and increase transcription. This part is incompatible with environments containing lactose or lactose analogs.
star: true
creator: Neelaksh Varshney, Grace Kenney, Daniel Shen, Samantha Sutton
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0011
sequence annotation: lac O1
sequence annotation: -35
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0011_sequence/1

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation7064
display id: annotation7064
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation7064/range7064/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0011/annotation7064/range7064/1
type: Range
persistent identity: range7064
display id: range7064
version: 1
top level: lacI+pL
direction: direction/forward
start: 1
end: 54
orientation: inline

lac O1
type: SequenceAnnotation
persistent identity: annotation1999
display id: annotation1999
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation1999/range1999/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_R0011/annotation1999/range1999/1
type: Range
persistent identity: range1999
display id: range1999
version: 1
top level: lacI+pL
direction: direction/forward
start: 3
end: 19
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2000
display id: annotation2000
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation2000/range2000/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0011/annotation2000/range2000/1
type: Range
persistent identity: range2000
display id: range2000
version: 1
top level: lacI+pL
direction: direction/forward
start: 20
end: 25
orientation: inline

lac O1
type: SequenceAnnotation
persistent identity: annotation2001
display id: annotation2001
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation2001/range2001/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_R0011/annotation2001/range2001/1
type: Range
persistent identity: range2001
display id: range2001
version: 1
top level: lacI+pL
direction: direction/forward
start: 26
end: 42
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2002
display id: annotation2002
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation2002/range2002/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0011/annotation2002/range2002/1
type: Range
persistent identity: range2002
display id: range2002
version: 1
top level: lacI+pL
direction: direction/forward
start: 43
end: 48
orientation: inline

https://synbiohub.org/public/igem/BBa_J32029_sequence/1
type: Sequence
persistent identity: BBa J32029 sequence
display id: BBa_J32029_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagaaagaggagaaatactagatgactaaaaaaatttcattcattattaacggccaggttgaaatctttcccgaaagtgatgatttagtgcaatccattaattttggtgataatagtgtttacctgccaatattgaatgactctcatgtaaaaaacattattgattgtaatggaaataacgaattacggttgcataacattgtcaattttctctatacggtagggcaaagatggaaaaatgaagaatactcaagacgcaggacatacattcgtgacttaaaaaaatatatgggatattcagaagaaatggctaagctagaggccaattggatatctatgattttatgttctaaaggcggcctttatgatgttgtagaaaatgaacttggttctcgccatatcatggatgaatggctacctcaggatgaaagttatgttcgggcttttccgaaaggtaaatctgtacatctgttggcaggtaatgttccattatctgggatcatgtctatattacgcgcaattttaactaagaatcagtgtattataaaaacatcgtcaaccgatccttttaccgctaatgcattagcgttaagttttattgatgtagaccctaatcatccgataacgcgctctttatctgttatatattggccccaccaaggtgatacatcactcgcaaaagaaattatgcgacatgcggatgttattgtcgcttggggagggccagatgcgattaattgggcggtagagcatgcgccatcttatgctgatgtgattaaatttggttctaaaaagagtctttgcattatcgataatcctgttgatttgacgtccgcagcgacaggtgcggctcatgatgtttgtttttacgatcagcgagcttgtttttctgcccaaaacatatattacatgggaaatcattatgaggaatttaagttagcgttgatagaaaaacttaatctatatgcgcatatattaccgaatgccaaaaaagattttgatgaaaaggcggcctattctttagttcaaaaagaaagcttgtttgctggattaaaagtagaggtggatattcatcaacgttggatgattattgagtcaaatgcaggtgtggaatttaatcaaccacttggcagatgtgtgtaccttcatcacgtcgataatattgagcaaatattgccttatgttcaaaaaaataagacgcaaaccatatctatttttccttgggagtcatcatttaaatatcgagatgcgttagcattaaaaggtgcggaaaggattgtagaagcaggaatgaataacatatttcgagttggtggatctcatgacggaatgagaccgttgcaacgattagtgacatatatttctcatgaaaggccatctaactatacggctaaggatgttgcggttgaaatagaacagactcgattcctggaagaagataagttccttgtatttgtcccataataggtaaaagtatggaaaatgaatcaaaatataaaaccatcgaccacgttatttgtgttgaaggaaataaaaaaattcatgtttgggaaacgctgccagaagaaaacagcccaaagagaaagaatgccattattattgcgtctggttttgcccgcaggatggatcattttgctggtctggcggaatatttatcgcggaatggatttcatgtgatccgctatgattcgcttcaccacgttggattgagttcagggacaattgatgaatttacaatgtctataggaaagcagagcttgttagcagtggttgattggttaactacacgaaaaataaataacttcggtatgttggcttcaagcttatctgcgcggatagcttatgcaagcctatctgaaatcaatgcttcgtttttaatcaccgcagtcggtgttgttaacttaagatattctcttgaaagagctttagggtttgattat…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J32007_sequence/1
type: Sequence
persistent identity: BBa J32007 sequence
display id: BBa_J32007_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: myers
owned by: james
elements: atgactaaaaaaatttcattcattattaacggccaggttgaaatctttcccgaaagtgatgatttagtgcaatccattaattttggtgataatagtgtttacctgccaatattgaatgactctcatgtaaaaaacattattgattgtaatggaaataacgaattacggttgcataacattgtcaattttctctatacggtagggcaaagatggaaaaatgaagaatactcaagacgcaggacatacattcgtgacttaaaaaaatatatgggatattcagaagaaatggctaagctagaggccaattggatatctatgattttatgttctaaaggcggcctttatgatgttgtagaaaatgaacttggttctcgccatatcatggatgaatggctacctcaggatgaaagttatgttcgggcttttccgaaaggtaaatctgtacatctgttggcaggtaatgttccattatctgggatcatgtctatattacgcgcaattttaactaagaatcagtgtattataaaaacatcgtcaaccgatccttttaccgctaatgcattagcgttaagttttattgatgtagaccctaatcatccgataacgcgctctttatctgttatatattggccccaccaaggtgatacatcactcgcaaaagaaattatgcgacatgcggatgttattgtcgcttggggagggccagatgcgattaattgggcggtagagcatgcgccatcttatgctgatgtgattaaatttggttctaaaaagagtctttgcattatcgataatcctgttgatttgacgtccgcagcgacaggtgcggctcatgatgtttgtttttacgatcagcgagcttgtttttctgcccaaaacatatattacatgggaaatcattatgaggaatttaagttagcgttgatagaaaaacttaatctatatgcgcatatattaccgaatgccaaaaaagattttgatgaaaaggcggcctattctttagttcaaaaagaaagcttgtttgctggattaaaagtagaggtggatattcatcaacgttggatgattattgagtcaaatgcaggtgtggaatttaatcaaccacttggcagatgtgtgtaccttcatcacgtcgataatattgagcaaatattgccttatgttcaaaaaaataagacgcaaaccatatctatttttccttgggagtcatcatttaaatatcgagatgcgttagcattaaaaggtgcggaaaggattgtagaagcaggaatgaataacatatttcgagttggtggatctcatgacggaatgagaccgttgcaacgattagtgacatatatttctcatgaaaggccatctaactatacggctaaggatgttgcggttgaaatagaacagactcgattcctggaagaagataagttccttgtatttgtcccataataggtaaaagtatggaaaatgaatcaaaatataaaaccatcgaccacgttatttgtgttgaaggaaataaaaaaattcatgtttgggaaacgctgccagaagaaaacagcccaaagagaaagaatgccattattattgcgtctggttttgcccgcaggatggatcattttgctggtctggcggaatatttatcgcggaatggatttcatgtgatccgctatgattcgcttcaccacgttggattgagttcagggacaattgatgaatttacaatgtctataggaaagcagagcttgttagcagtggttgattggttaactacacgaaaaataaataacttcggtatgttggcttcaagcttatctgcgcggatagcttatgcaagcctatctgaaatcaatgcttcgtttttaatcaccgcagtcggtgttgttaacttaagatattctcttgaaagagctttagggtttgattatctcagtctacccattaatgaattgccggataacctagattttgaaggccataaattgggtgctgaagtctttgcgagagat…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0011_sequence/1
type: Sequence
persistent identity: BBa R0011 sequence
display id: BBa_R0011_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcaca
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z