LOCUS BBa_J329001 2910 bp DNA linear 22-Dec-2024 DEFINITION Simple lux-based QS circuit expressing untagged GFP ACCESSION BBa_J329001 VERSION BBa_J329001.1 FEATURES Location/Qualifiers misc_feature 1..20 /label=LuxR/HSL promoter 115..122 /label=-10 promoter 89..94 /label=-35 misc_feature 128..128 /label=start misc_feature 130..149 /label=Putative LuxR/HSL misc_feature 1..151 /label=BBa_R0063 promoter 1..151 /label=BBa_R0063 misc_feature 164..167 /label=conserved RBS 160..171 /label=BBa_B0034 misc_feature 178..179 /label=prefix misc_feature 351..351 /label=T misc_feature 669..669 /label=A CDS 178..927 /label=luxR misc_feature 178..933 /label=BBa_C0062 misc_feature 934..958 /label=Help:Barcodes CDS 178..933 /label=BBa_C0062 stem_loop 978..1021 /label=stem_loop misc_feature 967..1046 /label=BBa_B0010 terminator 967..1046 /label=BBa_B0010 stem_loop 1062..1081 /label=T7 TE misc_feature 1088..1088 /label=stop polyA_site 1082..1095 /label=polya misc_feature 1055..1095 /label=BBa_B0012 terminator 1055..1095 /label=BBa_B0012 misc_feature 1104..1123 /label=LuxR/HSL promoter 1123..1128 /label=-35 promoter 1145..1150 /label=-10 misc_feature 1156..1156 /label=start misc_feature 1104..1158 /label=BBa_R0062 promoter 1104..1158 /label=BBa_R0062 misc_feature 1..1158 /label=BBa_F2621 misc_feature 1171..1174 /label=conserved RBS 1167..1178 /label=BBa_B0034 misc_feature 1764..1795 /label=LVA CDS 1185..1763 /label=luxI misc_feature 1185..1802 /label=BBa_C0061 misc_feature 1803..1827 /label=Help:Barcodes CDS 1185..1802 /label=BBa_C0061 misc_feature 1167..1827 /label=BBa_C0261 stem_loop 1847..1890 /label=stem_loop misc_feature 1836..1915 /label=BBa_B0010 terminator 1836..1915 /label=BBa_B0010 stem_loop 1931..1950 /label=T7 TE misc_feature 1957..1957 /label=stop polyA_site 1951..1964 /label=polya misc_feature 1924..1964 /label=BBa_B0012 terminator 1924..1964 /label=BBa_B0012 terminator 1836..1964 /label=BBa_B0015 misc_feature 1167..1964 /label=BBa_K082014 misc_feature 1..1964 /label=BBa_K546000 misc_feature 1973..1992 /label=LuxR/HSL promoter 1992..1997 /label=-35 promoter 2014..2019 /label=-10 misc_feature 2025..2025 /label=start misc_feature 1973..2027 /label=BBa_R0062 promoter 1973..2027 /label=BBa_R0062 misc_feature 2040..2043 /label=conserved RBS 2036..2047 /label=BBa_B0034 CDS 2054..2773 /label=GFP protein CDS 2054..2773 /label=BBa_E0040 stem_loop 2793..2836 /label=stem_loop misc_feature 2782..2861 /label=BBa_B0010 terminator 2782..2861 /label=BBa_B0010 stem_loop 2877..2896 /label=T7 TE misc_feature 2903..2903 /label=stop polyA_site 2897..2910 /label=polya misc_feature 2870..2910 /label=BBa_B0012 terminator 2870..2910 /label=BBa_B0012 misc_feature 1973..2910 /label=BBa_J37032 ORIGIN 1 acctgtacga tcctacaggt gcttatgtta agtaattgta ttcccagcga tacaatagtg 61 tgacaaaaat ccaatttatt agaatcaaat gtcaatccat taccgtttta atgatatata 121 acacgcaaaa cttgcgacaa acaataggta atactagaga aagaggagaa atactagatg 181 aaaaacataa atgccgacga cacatacaga ataattaata aaattaaagc ttgtagaagc 241 aataatgata ttaatcaatg cttatctgat atgactaaaa tggtacattg tgaatattat 301 ttactcgcga tcatttatcc tcattctatg gttaaatctg atatttcaat cctagataat 361 taccctaaaa aatggaggca atattatgat gacgctaatt taataaaata tgatcctata 421 gtagattatt ctaactccaa tcattcacca attaattgga atatatttga aaacaatgct 481 gtaaataaaa aatctccaaa tgtaattaaa gaagcgaaaa catcaggtct tatcactggg 541 tttagtttcc ctattcatac ggctaacaat ggcttcggaa tgcttagttt tgcacattca 601 gaaaaagaca actatataga tagtttattt ttacatgcgt gtatgaacat accattaatt 661 gttccttctc tagttgataa ttatcgaaaa ataaatatag caaataataa atcaaacaac 721 gatttaacca aaagagaaaa agaatgttta gcgtgggcat gcgaaggaaa aagctcttgg 781 gatatttcaa aaatattagg ttgcagtgag cgtactgtca ctttccattt aaccaatgcg 841 caaatgaaac tcaatacaac aaaccgctgc caaagtattt ctaaagcaat tttaacagga 901 gcaattgatt gcccatactt taaaaattaa taacactgat agtgctagtg tagatcacta 961 ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta 1021 tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc 1081 ctttctgcgt ttatatacta gagacctgta ggatcgtaca ggtttacgca agaaaatggt 1141 ttgttatagt cgaataaata ctagagaaag aggagaaata ctagatgact ataatgataa 1201 aaaaatcgga ttttttggca attccatcgg aggagtataa aggtattcta agtcttcgtt 1261 atcaagtgtt taagcaaaga cttgagtggg acttagttgt agaaaataac cttgaatcag 1321 atgagtatga taactcaaat gcagaatata tttatgcttg tgatgatact gaaaatgtaa 1381 gtggatgctg gcgtttatta cctacaacag gtgattatat gctgaaaagt gtttttcctg 1441 aattgcttgg tcaacagagt gctcccaaag atcctaatat agtcgaatta agtcgttttg 1501 ctgtaggtaa aaatagctca aagataaata actctgctag tgaaattaca atgaaactat 1561 ttgaagctat atataaacac gctgttagtc aaggtattac agaatatgta acagtaacat 1621 caacagcaat agagcgattt ttaaagcgta ttaaagttcc ttgtcatcgt attggagaca 1681 aagaaattca tgtattaggt gatactaaat cggttgtatt gtctatgcct attaatgaac 1741 agtttaaaaa agcagtctta aatgctgcaa acgacgaaaa ctacgcttta gtagcttaat 1801 aactctgata gtgctagtgt agatctctac tagagccagg catcaaataa aacgaaaggc 1861 tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta 1921 gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tatatactag agacctgtag 1981 gatcgtacag gtttacgcaa gaaaatggtt tgttatagtc gaataaatac tagagaaaga 2041 ggagaaatac tagatgcgta aaggagaaga acttttcact ggagttgtcc caattcttgt 2101 tgaattagat ggtgatgtta atgggcacaa attttctgtc agtggagagg gtgaaggtga 2161 tgcaacatac ggaaaactta cccttaaatt tatttgcact actggaaaac tacctgttcc 2221 atggccaaca cttgtcacta ctttcggtta tggtgttcaa tgctttgcga gatacccaga 2281 tcatatgaaa cagcatgact ttttcaagag tgccatgccc gaaggttatg tacaggaaag 2341 aactatattt ttcaaagatg acgggaacta caagacacgt gctgaagtca agtttgaagg 2401 tgataccctt gttaatagaa tcgagttaaa aggtattgat tttaaagaag atggaaacat 2461 tcttggacac aaattggaat acaactataa ctcacacaat gtatacatca tggcagacaa 2521 acaaaagaat ggaatcaaag ttaacttcaa aattagacac aacattgaag atggaagcgt 2581 tcaactagca gaccattatc aacaaaatac tccaattggc gatggccctg tccttttacc 2641 agacaaccat tacctgtcca cacaatctgc cctttcgaaa gatcccaacg aaaagagaga 2701 ccacatggtc cttcttgagt ttgtaacagc tgctgggatt acacatggca tggatgaact 2761 atacaaataa taatactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact 2821 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct 2881 caccttcggg tgggcctttc tgcgtttata //