LOCUS BBa_J329003 2852 bp DNA linear 25-Nov-2024 DEFINITION Simple Quorum Sensing System Expressing YFP ACCESSION BBa_J329003 VERSION BBa_J329003.1 FEATURES Location/Qualifiers misc_feature 1..20 /label=LuxR/HSL promoter 115..122 /label=-10 promoter 89..94 /label=-35 misc_feature 128..128 /label=start misc_feature 130..149 /label=Putative LuxR/HSL misc_feature 1..151 /label=BBa_R0063 promoter 1..151 /label=BBa_R0063 misc_feature 164..167 /label=conserved RBS 160..171 /label=BBa_B0034 misc_feature 178..179 /label=prefix misc_feature 351..351 /label=T misc_feature 669..669 /label=A CDS 178..927 /label=luxR misc_feature 178..933 /label=BBa_C0062 misc_feature 934..958 /label=Help:Barcodes CDS 178..933 /label=BBa_C0062 stem_loop 978..1021 /label=stem_loop misc_feature 967..1046 /label=BBa_B0010 terminator 967..1046 /label=BBa_B0010 stem_loop 1062..1081 /label=T7 TE misc_feature 1088..1088 /label=stop polyA_site 1082..1095 /label=polya misc_feature 1055..1095 /label=BBa_B0012 terminator 1055..1095 /label=BBa_B0012 misc_feature 1104..1123 /label=LuxR/HSL promoter 1123..1128 /label=-35 promoter 1145..1150 /label=-10 misc_feature 1156..1156 /label=start misc_feature 1104..1158 /label=BBa_R0062 promoter 1104..1158 /label=BBa_R0062 misc_feature 1171..1174 /label=conserved RBS 1167..1178 /label=BBa_B0034 CDS 1185..1907 /label=BBa_E0030 stem_loop 1927..1970 /label=stem_loop misc_feature 1916..1995 /label=BBa_B0010 terminator 1916..1995 /label=BBa_B0010 stem_loop 2011..2030 /label=T7 TE misc_feature 2037..2037 /label=stop polyA_site 2031..2044 /label=polya misc_feature 2004..2044 /label=BBa_B0012 terminator 2004..2044 /label=BBa_B0012 misc_feature 1..2044 /label=BBa_I13263 misc_feature 2059..2062 /label=conserved RBS 2053..2066 /label=BBa_B0031 misc_feature 2652..2683 /label=LVA CDS 2073..2651 /label=luxI misc_feature 2073..2690 /label=BBa_C0061 misc_feature 2691..2715 /label=Help:Barcodes CDS 2073..2690 /label=BBa_C0061 misc_feature 2053..2715 /label=BBa_K516011 stem_loop 2735..2778 /label=stem_loop misc_feature 2724..2803 /label=BBa_B0010 terminator 2724..2803 /label=BBa_B0010 stem_loop 2819..2838 /label=T7 TE misc_feature 2845..2845 /label=stop polyA_site 2839..2852 /label=polya misc_feature 2812..2852 /label=BBa_B0012 terminator 2812..2852 /label=BBa_B0012 terminator 2724..2852 /label=BBa_B0015 misc_feature 2053..2852 /label=BBa_J329002 ORIGIN 1 acctgtacga tcctacaggt gcttatgtta agtaattgta ttcccagcga tacaatagtg 61 tgacaaaaat ccaatttatt agaatcaaat gtcaatccat taccgtttta atgatatata 121 acacgcaaaa cttgcgacaa acaataggta atactagaga aagaggagaa atactagatg 181 aaaaacataa atgccgacga cacatacaga ataattaata aaattaaagc ttgtagaagc 241 aataatgata ttaatcaatg cttatctgat atgactaaaa tggtacattg tgaatattat 301 ttactcgcga tcatttatcc tcattctatg gttaaatctg atatttcaat cctagataat 361 taccctaaaa aatggaggca atattatgat gacgctaatt taataaaata tgatcctata 421 gtagattatt ctaactccaa tcattcacca attaattgga atatatttga aaacaatgct 481 gtaaataaaa aatctccaaa tgtaattaaa gaagcgaaaa catcaggtct tatcactggg 541 tttagtttcc ctattcatac ggctaacaat ggcttcggaa tgcttagttt tgcacattca 601 gaaaaagaca actatataga tagtttattt ttacatgcgt gtatgaacat accattaatt 661 gttccttctc tagttgataa ttatcgaaaa ataaatatag caaataataa atcaaacaac 721 gatttaacca aaagagaaaa agaatgttta gcgtgggcat gcgaaggaaa aagctcttgg 781 gatatttcaa aaatattagg ttgcagtgag cgtactgtca ctttccattt aaccaatgcg 841 caaatgaaac tcaatacaac aaaccgctgc caaagtattt ctaaagcaat tttaacagga 901 gcaattgatt gcccatactt taaaaattaa taacactgat agtgctagtg tagatcacta 961 ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta 1021 tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc 1081 ctttctgcgt ttatatacta gagacctgta ggatcgtaca ggtttacgca agaaaatggt 1141 ttgttatagt cgaataaata ctagagaaag aggagaaata ctagatggtg agcaagggcg 1201 aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc 1261 acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga 1321 agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccttcg 1381 gctacggcct gcaatgcttc gcccgctacc ccgaccacat gaagctgcac gacttcttca 1441 agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca 1501 actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc 1561 tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact 1621 acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact 1681 tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga 1741 acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agctaccagt 1801 ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga 1861 ccgccgccgg gatcactctc ggcatggacg agctgtacaa gtaataatac tagagccagg 1921 catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg 1981 tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt 2041 tatatactag agtcacacag gaaacctact agatgactat aatgataaaa aaatcggatt 2101 ttttggcaat tccatcggag gagtataaag gtattctaag tcttcgttat caagtgttta 2161 agcaaagact tgagtgggac ttagttgtag aaaataacct tgaatcagat gagtatgata 2221 actcaaatgc agaatatatt tatgcttgtg atgatactga aaatgtaagt ggatgctggc 2281 gtttattacc tacaacaggt gattatatgc tgaaaagtgt ttttcctgaa ttgcttggtc 2341 aacagagtgc tcccaaagat cctaatatag tcgaattaag tcgttttgct gtaggtaaaa 2401 atagctcaaa gataaataac tctgctagtg aaattacaat gaaactattt gaagctatat 2461 ataaacacgc tgttagtcaa ggtattacag aatatgtaac agtaacatca acagcaatag 2521 agcgattttt aaagcgtatt aaagttcctt gtcatcgtat tggagacaaa gaaattcatg 2581 tattaggtga tactaaatcg gttgtattgt ctatgcctat taatgaacag tttaaaaaag 2641 cagtcttaaa tgctgcaaac gacgaaaact acgctttagt agcttaataa ctctgatagt 2701 gctagtgtag atctctacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga 2761 ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg 2821 ctcaccttcg ggtgggcctt tctgcgttta ta //