LOCUS BBa_J329005 863 bp DNA linear 22-Nov-2024 DEFINITION LuxI for RBS Swapping ACCESSION BBa_J329005 VERSION BBa_J329005.1 FEATURES Location/Qualifiers misc_feature 1..20 /label=LuxR/HSL promoter 20..25 /label=-35 promoter 42..47 /label=-10 misc_feature 53..53 /label=start misc_feature 1..55 /label=BBa_R0062 promoter 1..55 /label=BBa_R0062 misc_feature 70..73 /label=conserved RBS 64..77 /label=BBa_B0031 misc_feature 663..694 /label=LVA CDS 84..662 /label=luxI misc_feature 84..701 /label=BBa_C0061 misc_feature 702..726 /label=Help:Barcodes CDS 84..701 /label=BBa_C0061 misc_feature 64..726 /label=BBa_K516011 stem_loop 746..789 /label=stem_loop misc_feature 735..814 /label=BBa_B0010 terminator 735..814 /label=BBa_B0010 stem_loop 830..849 /label=T7 TE misc_feature 856..856 /label=stop polyA_site 850..863 /label=polya misc_feature 823..863 /label=BBa_B0012 terminator 823..863 /label=BBa_B0012 terminator 735..863 /label=BBa_B0015 ORIGIN 1 acctgtagga tcgtacaggt ttacgcaaga aaatggtttg ttatagtcga ataaatacta 61 gagtcacaca ggaaacctac tagatgacta taatgataaa aaaatcggat tttttggcaa 121 ttccatcgga ggagtataaa ggtattctaa gtcttcgtta tcaagtgttt aagcaaagac 181 ttgagtggga cttagttgta gaaaataacc ttgaatcaga tgagtatgat aactcaaatg 241 cagaatatat ttatgcttgt gatgatactg aaaatgtaag tggatgctgg cgtttattac 301 ctacaacagg tgattatatg ctgaaaagtg tttttcctga attgcttggt caacagagtg 361 ctcccaaaga tcctaatata gtcgaattaa gtcgttttgc tgtaggtaaa aatagctcaa 421 agataaataa ctctgctagt gaaattacaa tgaaactatt tgaagctata tataaacacg 481 ctgttagtca aggtattaca gaatatgtaa cagtaacatc aacagcaata gagcgatttt 541 taaagcgtat taaagttcct tgtcatcgta ttggagacaa agaaattcat gtattaggtg 601 atactaaatc ggttgtattg tctatgccta ttaatgaaca gtttaaaaaa gcagtcttaa 661 atgctgcaaa cgacgaaaac tacgctttag tagcttaata actctgatag tgctagtgta 721 gatctctact agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt 781 tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc 841 gggtgggcct ttctgcgttt ata //