LOCUS BBa_J329006 3045 bp DNA linear 22-Nov-2024 DEFINITION Simple QS Circuit with GFP Controlled by LuxR Promoter ACCESSION BBa_J329006 VERSION BBa_J329006.1 FEATURES Location/Qualifiers misc_feature 1..20 /label=LuxR/HSL promoter 115..122 /label=-10 promoter 89..94 /label=-35 misc_feature 128..128 /label=start misc_feature 130..149 /label=Putative LuxR/HSL misc_feature 1..151 /label=BBa_R0063 promoter 1..151 /label=BBa_R0063 misc_feature 164..167 /label=conserved RBS 160..171 /label=BBa_B0034 misc_feature 178..179 /label=prefix misc_feature 351..351 /label=T misc_feature 669..669 /label=A CDS 178..927 /label=luxR misc_feature 178..933 /label=BBa_C0062 misc_feature 934..958 /label=Help:Barcodes CDS 178..933 /label=BBa_C0062 stem_loop 978..1021 /label=stem_loop misc_feature 967..1046 /label=BBa_B0010 terminator 967..1046 /label=BBa_B0010 stem_loop 1062..1081 /label=T7 TE misc_feature 1088..1088 /label=stop polyA_site 1082..1095 /label=polya misc_feature 1055..1095 /label=BBa_B0012 terminator 1055..1095 /label=BBa_B0012 misc_feature 1104..1123 /label=LuxR/HSL promoter 1123..1128 /label=-35 promoter 1145..1150 /label=-10 misc_feature 1156..1156 /label=start misc_feature 1104..1158 /label=BBa_R0062 promoter 1104..1158 /label=BBa_R0062 misc_feature 1..1158 /label=BBa_F2621 misc_feature 1171..1174 /label=conserved RBS 1167..1178 /label=BBa_B0034 misc_feature 1764..1795 /label=LVA CDS 1185..1763 /label=luxI misc_feature 1185..1802 /label=BBa_C0061 misc_feature 1803..1827 /label=Help:Barcodes CDS 1185..1802 /label=BBa_C0061 misc_feature 1167..1827 /label=BBa_C0261 stem_loop 1847..1890 /label=stem_loop misc_feature 1836..1915 /label=BBa_B0010 terminator 1836..1915 /label=BBa_B0010 stem_loop 1931..1950 /label=T7 TE misc_feature 1957..1957 /label=stop polyA_site 1951..1964 /label=polya misc_feature 1924..1964 /label=BBa_B0012 terminator 1924..1964 /label=BBa_B0012 terminator 1836..1964 /label=BBa_B0015 misc_feature 1167..1964 /label=BBa_K082014 misc_feature 1..1964 /label=BBa_K546000 misc_feature 1973..1992 /label=LuxR/HSL promoter 2087..2094 /label=-10 promoter 2061..2066 /label=-35 misc_feature 2100..2100 /label=start misc_feature 2102..2121 /label=Putative LuxR/HSL misc_feature 1973..2123 /label=BBa_R0063 promoter 1973..2123 /label=BBa_R0063 misc_feature 2136..2139 /label=conserved RBS 2132..2143 /label=BBa_B0034 misc_feature 2150..2869 /label=BBa_E0040 misc_feature 2864..2902 /label=LVA misc_feature 2903..2908 /label=Double Stop misc_feature 2150..2152 /label=Start misc_feature 2150..2908 /label=BBa_J04031 stem_loop 2928..2971 /label=stem_loop misc_feature 2917..2996 /label=BBa_B0010 terminator 2917..2996 /label=BBa_B0010 stem_loop 3012..3031 /label=T7 TE misc_feature 3038..3038 /label=stop polyA_site 3032..3045 /label=polya misc_feature 3005..3045 /label=BBa_B0012 terminator 3005..3045 /label=BBa_B0012 misc_feature 2132..3045 /label=BBa_I763020 ORIGIN 1 acctgtacga tcctacaggt gcttatgtta agtaattgta ttcccagcga tacaatagtg 61 tgacaaaaat ccaatttatt agaatcaaat gtcaatccat taccgtttta atgatatata 121 acacgcaaaa cttgcgacaa acaataggta atactagaga aagaggagaa atactagatg 181 aaaaacataa atgccgacga cacatacaga ataattaata aaattaaagc ttgtagaagc 241 aataatgata ttaatcaatg cttatctgat atgactaaaa tggtacattg tgaatattat 301 ttactcgcga tcatttatcc tcattctatg gttaaatctg atatttcaat cctagataat 361 taccctaaaa aatggaggca atattatgat gacgctaatt taataaaata tgatcctata 421 gtagattatt ctaactccaa tcattcacca attaattgga atatatttga aaacaatgct 481 gtaaataaaa aatctccaaa tgtaattaaa gaagcgaaaa catcaggtct tatcactggg 541 tttagtttcc ctattcatac ggctaacaat ggcttcggaa tgcttagttt tgcacattca 601 gaaaaagaca actatataga tagtttattt ttacatgcgt gtatgaacat accattaatt 661 gttccttctc tagttgataa ttatcgaaaa ataaatatag caaataataa atcaaacaac 721 gatttaacca aaagagaaaa agaatgttta gcgtgggcat gcgaaggaaa aagctcttgg 781 gatatttcaa aaatattagg ttgcagtgag cgtactgtca ctttccattt aaccaatgcg 841 caaatgaaac tcaatacaac aaaccgctgc caaagtattt ctaaagcaat tttaacagga 901 gcaattgatt gcccatactt taaaaattaa taacactgat agtgctagtg tagatcacta 961 ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta 1021 tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc 1081 ctttctgcgt ttatatacta gagacctgta ggatcgtaca ggtttacgca agaaaatggt 1141 ttgttatagt cgaataaata ctagagaaag aggagaaata ctagatgact ataatgataa 1201 aaaaatcgga ttttttggca attccatcgg aggagtataa aggtattcta agtcttcgtt 1261 atcaagtgtt taagcaaaga cttgagtggg acttagttgt agaaaataac cttgaatcag 1321 atgagtatga taactcaaat gcagaatata tttatgcttg tgatgatact gaaaatgtaa 1381 gtggatgctg gcgtttatta cctacaacag gtgattatat gctgaaaagt gtttttcctg 1441 aattgcttgg tcaacagagt gctcccaaag atcctaatat agtcgaatta agtcgttttg 1501 ctgtaggtaa aaatagctca aagataaata actctgctag tgaaattaca atgaaactat 1561 ttgaagctat atataaacac gctgttagtc aaggtattac agaatatgta acagtaacat 1621 caacagcaat agagcgattt ttaaagcgta ttaaagttcc ttgtcatcgt attggagaca 1681 aagaaattca tgtattaggt gatactaaat cggttgtatt gtctatgcct attaatgaac 1741 agtttaaaaa agcagtctta aatgctgcaa acgacgaaaa ctacgcttta gtagcttaat 1801 aactctgata gtgctagtgt agatctctac tagagccagg catcaaataa aacgaaaggc 1861 tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta 1921 gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tatatactag agacctgtac 1981 gatcctacag gtgcttatgt taagtaattg tattcccagc gatacaatag tgtgacaaaa 2041 atccaattta ttagaatcaa atgtcaatcc attaccgttt taatgatata taacacgcaa 2101 aacttgcgac aaacaatagg taatactaga gaaagaggag aaatactaga tgcgtaaagg 2161 agaagaactt ttcactggag ttgtcccaat tcttgttgaa ttagatggtg atgttaatgg 2221 gcacaaattt tctgtcagtg gagagggtga aggtgatgca acatacggaa aacttaccct 2281 taaatttatt tgcactactg gaaaactacc tgttccatgg ccaacacttg tcactacttt 2341 cggttatggt gttcaatgct ttgcgagata cccagatcat atgaaacagc atgacttttt 2401 caagagtgcc atgcccgaag gttatgtaca ggaaagaact atatttttca aagatgacgg 2461 gaactacaag acacgtgctg aagtcaagtt tgaaggtgat acccttgtta atagaatcga 2521 gttaaaaggt attgatttta aagaagatgg aaacattctt ggacacaaat tggaatacaa 2581 ctataactca cacaatgtat acatcatggc agacaaacaa aagaatggaa tcaaagttaa 2641 cttcaaaatt agacacaaca ttgaagatgg aagcgttcaa ctagcagacc attatcaaca 2701 aaatactcca attggcgatg gccctgtcct tttaccagac aaccattacc tgtccacaca 2761 atctgccctt tcgaaagatc ccaacgaaaa gagagaccac atggtccttc ttgagtttgt 2821 aacagctgct gggattacac atggcatgga tgaactatac aaaaggcctg ctgcaaacga 2881 cgaaaactac gctttagtag cttaataata ctagagccag gcatcaaata aaacgaaagg 2941 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact 3001 agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttata //