LOCUS BBa_J329021 3856 bp DNA linear 28-Nov-2024 DEFINITION LuxIR QS System with Double luxI with weaker RBS ACCESSION BBa_J329021 VERSION BBa_J329021.1 FEATURES Location/Qualifiers misc_feature 1..20 /label=LuxR/HSL promoter 115..122 /label=-10 promoter 89..94 /label=-35 misc_feature 128..128 /label=start misc_feature 130..149 /label=Putative LuxR/HSL misc_feature 1..151 /label=BBa_R0063 promoter 1..151 /label=BBa_R0063 misc_feature 164..167 /label=conserved RBS 160..171 /label=BBa_B0034 misc_feature 178..179 /label=prefix misc_feature 351..351 /label=T misc_feature 669..669 /label=A CDS 178..927 /label=luxR misc_feature 178..933 /label=BBa_C0062 misc_feature 934..958 /label=Help:Barcodes CDS 178..933 /label=BBa_C0062 stem_loop 978..1021 /label=stem_loop misc_feature 967..1046 /label=BBa_B0010 terminator 967..1046 /label=BBa_B0010 stem_loop 1062..1081 /label=T7 TE misc_feature 1088..1088 /label=stop polyA_site 1082..1095 /label=polya misc_feature 1055..1095 /label=BBa_B0012 terminator 1055..1095 /label=BBa_B0012 terminator 967..1095 /label=BBa_B0015 misc_feature 1104..1123 /label=LuxR/HSL promoter 1123..1128 /label=-35 promoter 1145..1150 /label=-10 misc_feature 1156..1156 /label=start misc_feature 1104..1158 /label=BBa_R0062 promoter 1104..1158 /label=BBa_R0062 RBS 1167..1197 /label=BBa_J329999 misc_feature 1783..1814 /label=LVA CDS 1204..1782 /label=luxI misc_feature 1204..1821 /label=BBa_C0061 misc_feature 1822..1846 /label=Help:Barcodes CDS 1204..1821 /label=BBa_C0061 stem_loop 1866..1909 /label=stem_loop misc_feature 1855..1934 /label=BBa_B0010 terminator 1855..1934 /label=BBa_B0010 stem_loop 1950..1969 /label=T7 TE misc_feature 1976..1976 /label=stop polyA_site 1970..1983 /label=polya misc_feature 1943..1983 /label=BBa_B0012 terminator 1943..1983 /label=BBa_B0012 terminator 1855..1983 /label=BBa_B0015 misc_feature 1992..2011 /label=LuxR/HSL promoter 2011..2016 /label=-35 promoter 2033..2038 /label=-10 misc_feature 2044..2044 /label=start misc_feature 1992..2046 /label=BBa_R0062 promoter 1992..2046 /label=BBa_R0062 misc_feature 2059..2062 /label=conserved RBS 2055..2066 /label=BBa_B0034 misc_feature 2073..2792 /label=BBa_E0040 misc_feature 2787..2825 /label=LVA misc_feature 2826..2831 /label=Double Stop misc_feature 2073..2075 /label=Start misc_feature 2073..2831 /label=BBa_J04031 stem_loop 2851..2894 /label=stem_loop misc_feature 2840..2919 /label=BBa_B0010 terminator 2840..2919 /label=BBa_B0010 stem_loop 2935..2954 /label=T7 TE misc_feature 2961..2961 /label=stop polyA_site 2955..2968 /label=polya misc_feature 2928..2968 /label=BBa_B0012 terminator 2928..2968 /label=BBa_B0012 misc_feature 2055..2968 /label=BBa_I763020 misc_feature 1..2968 /label=BBa_J329010 misc_feature 2977..2996 /label=LuxR/HSL promoter 2996..3001 /label=-35 promoter 3018..3023 /label=-10 misc_feature 3029..3029 /label=start misc_feature 2977..3031 /label=BBa_R0062 promoter 2977..3031 /label=BBa_R0062 RBS 3040..3070 /label=BBa_J329999 misc_feature 3656..3687 /label=LVA CDS 3077..3655 /label=luxI misc_feature 3077..3694 /label=BBa_C0061 misc_feature 3695..3719 /label=Help:Barcodes CDS 3077..3694 /label=BBa_C0061 stem_loop 3739..3782 /label=stem_loop misc_feature 3728..3807 /label=BBa_B0010 terminator 3728..3807 /label=BBa_B0010 stem_loop 3823..3842 /label=T7 TE misc_feature 3849..3849 /label=stop polyA_site 3843..3856 /label=polya misc_feature 3816..3856 /label=BBa_B0012 terminator 3816..3856 /label=BBa_B0012 terminator 3728..3856 /label=BBa_B0015 ORIGIN 1 acctgtacga tcctacaggt gcttatgtta agtaattgta ttcccagcga tacaatagtg 61 tgacaaaaat ccaatttatt agaatcaaat gtcaatccat taccgtttta atgatatata 121 acacgcaaaa cttgcgacaa acaataggta atactagaga aagaggagaa atactagatg 181 aaaaacataa atgccgacga cacatacaga ataattaata aaattaaagc ttgtagaagc 241 aataatgata ttaatcaatg cttatctgat atgactaaaa tggtacattg tgaatattat 301 ttactcgcga tcatttatcc tcattctatg gttaaatctg atatttcaat cctagataat 361 taccctaaaa aatggaggca atattatgat gacgctaatt taataaaata tgatcctata 421 gtagattatt ctaactccaa tcattcacca attaattgga atatatttga aaacaatgct 481 gtaaataaaa aatctccaaa tgtaattaaa gaagcgaaaa catcaggtct tatcactggg 541 tttagtttcc ctattcatac ggctaacaat ggcttcggaa tgcttagttt tgcacattca 601 gaaaaagaca actatataga tagtttattt ttacatgcgt gtatgaacat accattaatt 661 gttccttctc tagttgataa ttatcgaaaa ataaatatag caaataataa atcaaacaac 721 gatttaacca aaagagaaaa agaatgttta gcgtgggcat gcgaaggaaa aagctcttgg 781 gatatttcaa aaatattagg ttgcagtgag cgtactgtca ctttccattt aaccaatgcg 841 caaatgaaac tcaatacaac aaaccgctgc caaagtattt ctaaagcaat tttaacagga 901 gcaattgatt gcccatactt taaaaattaa taacactgat agtgctagtg tagatcacta 961 ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta 1021 tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc 1081 ctttctgcgt ttatatacta gagacctgta ggatcgtaca ggtttacgca agaaaatggt 1141 ttgttatagt cgaataaata ctagagagta ataagtcaag cgcactacaa atcccgttac 1201 tagatgacta taatgataaa aaaatcggat tttttggcaa ttccatcgga ggagtataaa 1261 ggtattctaa gtcttcgtta tcaagtgttt aagcaaagac ttgagtggga cttagttgta 1321 gaaaataacc ttgaatcaga tgagtatgat aactcaaatg cagaatatat ttatgcttgt 1381 gatgatactg aaaatgtaag tggatgctgg cgtttattac ctacaacagg tgattatatg 1441 ctgaaaagtg tttttcctga attgcttggt caacagagtg ctcccaaaga tcctaatata 1501 gtcgaattaa gtcgttttgc tgtaggtaaa aatagctcaa agataaataa ctctgctagt 1561 gaaattacaa tgaaactatt tgaagctata tataaacacg ctgttagtca aggtattaca 1621 gaatatgtaa cagtaacatc aacagcaata gagcgatttt taaagcgtat taaagttcct 1681 tgtcatcgta ttggagacaa agaaattcat gtattaggtg atactaaatc ggttgtattg 1741 tctatgccta ttaatgaaca gtttaaaaaa gcagtcttaa atgctgcaaa cgacgaaaac 1801 tacgctttag tagcttaata actctgatag tgctagtgta gatctctact agagccaggc 1861 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt 1921 cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt 1981 atatactaga gacctgtagg atcgtacagg tttacgcaag aaaatggttt gttatagtcg 2041 aataaatact agagaaagag gagaaatact agatgcgtaa aggagaagaa cttttcactg 2101 gagttgtccc aattcttgtt gaattagatg gtgatgttaa tgggcacaaa ttttctgtca 2161 gtggagaggg tgaaggtgat gcaacatacg gaaaacttac ccttaaattt atttgcacta 2221 ctggaaaact acctgttcca tggccaacac ttgtcactac tttcggttat ggtgttcaat 2281 gctttgcgag atacccagat catatgaaac agcatgactt tttcaagagt gccatgcccg 2341 aaggttatgt acaggaaaga actatatttt tcaaagatga cgggaactac aagacacgtg 2401 ctgaagtcaa gtttgaaggt gatacccttg ttaatagaat cgagttaaaa ggtattgatt 2461 ttaaagaaga tggaaacatt cttggacaca aattggaata caactataac tcacacaatg 2521 tatacatcat ggcagacaaa caaaagaatg gaatcaaagt taacttcaaa attagacaca 2581 acattgaaga tggaagcgtt caactagcag accattatca acaaaatact ccaattggcg 2641 atggccctgt ccttttacca gacaaccatt acctgtccac acaatctgcc ctttcgaaag 2701 atcccaacga aaagagagac cacatggtcc ttcttgagtt tgtaacagct gctgggatta 2761 cacatggcat ggatgaacta tacaaaaggc ctgctgcaaa cgacgaaaac tacgctttag 2821 tagcttaata atactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg 2881 gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca 2941 ccttcgggtg ggcctttctg cgtttatata ctagagacct gtaggatcgt acaggtttac 3001 gcaagaaaat ggtttgttat agtcgaataa atactagaga gtaataagtc aagcgcacta 3061 caaatcccgt tactagatga ctataatgat aaaaaaatcg gattttttgg caattccatc 3121 ggaggagtat aaaggtattc taagtcttcg ttatcaagtg tttaagcaaa gacttgagtg 3181 ggacttagtt gtagaaaata accttgaatc agatgagtat gataactcaa atgcagaata 3241 tatttatgct tgtgatgata ctgaaaatgt aagtggatgc tggcgtttat tacctacaac 3301 aggtgattat atgctgaaaa gtgtttttcc tgaattgctt ggtcaacaga gtgctcccaa 3361 agatcctaat atagtcgaat taagtcgttt tgctgtaggt aaaaatagct caaagataaa 3421 taactctgct agtgaaatta caatgaaact atttgaagct atatataaac acgctgttag 3481 tcaaggtatt acagaatatg taacagtaac atcaacagca atagagcgat ttttaaagcg 3541 tattaaagtt ccttgtcatc gtattggaga caaagaaatt catgtattag gtgatactaa 3601 atcggttgta ttgtctatgc ctattaatga acagtttaaa aaagcagtct taaatgctgc 3661 aaacgacgaa aactacgctt tagtagctta ataactctga tagtgctagt gtagatctct 3721 actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt 3781 atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg 3841 cctttctgcg tttata //