##gff-version 3
##sequence-region BBa_J329021 1 3856
BBa_J329021	.	engineered_region	1	2968	.	+	0	ID=BBa_J329010;Name=BBa_J329010
BBa_J329021	.	promoter	1104	1158	.	+	0	ID=BBa_R0062;Name=BBa_R0062;Parent=BBa_J329010
BBa_J329021	.	engineered_region	1104	1158	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_J329021	.	promoter	1123	1128	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_J329021	.	promoter	1145	1150	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_J329021	.	start_codon	1156	1156	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_J329021	.	non_covalent_binding_site	1104	1123	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_J329021	.	terminator	1855	1983	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_J329010
BBa_J329021	.	terminator	1943	1983	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_J329021	.	polyA_site	1970	1983	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J329021	.	engineered_region	1943	1983	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J329021	.	stem_loop	1950	1969	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J329021	.	stop_codon	1976	1976	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J329021	.	terminator	1855	1934	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_J329021	.	stem_loop	1866	1909	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J329021	.	engineered_region	1855	1934	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J329021	.	CDS	178	933	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_J329010
BBa_J329021	.	engineered_tag	934	958	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_J329021	.	engineered_region	178	933	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_J329021	.	CDS	178	927	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_J329021	.	point_mutation	351	351	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_J329021	.	point_mutation	669	669	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_J329021	.	engineered_region	178	179	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_J329021	.	terminator	967	1095	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_J329010
BBa_J329021	.	terminator	1055	1095	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_J329021	.	polyA_site	1082	1095	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J329021	.	engineered_region	1055	1095	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J329021	.	stem_loop	1062	1081	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J329021	.	stop_codon	1088	1088	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J329021	.	terminator	967	1046	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_J329021	.	stem_loop	978	1021	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J329021	.	engineered_region	967	1046	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J329021	.	ribosome_entry_site	1167	1197	.	+	0	ID=BBa_J329999;Name=BBa_J329999;Parent=BBa_J329010
BBa_J329021	.	ribosome_entry_site	160	171	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J329010
BBa_J329021	.	conserved_region	164	167	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J329021	.	promoter	1	151	.	+	0	ID=BBa_R0063;Name=BBa_R0063;Parent=BBa_J329010
BBa_J329021	.	start_codon	128	128	.	+	0	ID=annotation2054;Name=start;Parent=BBa_R0063
BBa_J329021	.	engineered_region	1	151	.	+	0	ID=annotation7071;Name=BBa_R0063;Parent=BBa_R0063
BBa_J329021	.	promoter	89	94	.	+	0	ID=annotation2053;Name=-35;Parent=BBa_R0063
BBa_J329021	.	non_covalent_binding_site	1	20	.	+	0	ID=annotation2051;Name=LuxR/HSL;Parent=BBa_R0063
BBa_J329021	.	non_covalent_binding_site	130	149	.	+	0	ID=annotation2055;Name=Putative LuxR/HSL;Parent=BBa_R0063
BBa_J329021	.	promoter	115	122	.	+	0	ID=annotation2052;Name=-10;Parent=BBa_R0063
BBa_J329021	.	engineered_region	2055	2968	.	+	0	ID=BBa_I763020;Name=BBa_I763020;Parent=BBa_J329010
BBa_J329021	.	engineered_region	2073	2831	.	+	0	ID=BBa_J04031;Name=BBa_J04031;Parent=BBa_I763020
BBa_J329021	.	start_codon	2073	2075	.	+	0	ID=annotation1507246;Name=Start;Parent=BBa_J04031
BBa_J329021	.	tag	2787	2825	.	+	0	ID=annotation1507235;Name=LVA;Parent=BBa_J04031
BBa_J329021	.	engineered_region	2073	2792	.	+	0	ID=annotation1507202;Name=BBa_E0040;Parent=BBa_J04031
BBa_J329021	.	stop_codon	2826	2831	.	+	0	ID=annotation1507242;Name=Double Stop;Parent=BBa_J04031
BBa_J329021	.	ribosome_entry_site	2055	2066	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I763020
BBa_J329021	.	conserved_region	2059	2062	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J329021	.	terminator	2840	2919	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I763020
BBa_J329021	.	stem_loop	2851	2894	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J329021	.	engineered_region	2840	2919	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J329021	.	terminator	2928	2968	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I763020
BBa_J329021	.	polyA_site	2955	2968	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J329021	.	engineered_region	2928	2968	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J329021	.	stem_loop	2935	2954	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J329021	.	stop_codon	2961	2961	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J329021	.	CDS	1204	1821	.	+	0	ID=BBa_C0061;Name=BBa_C0061;Parent=BBa_J329010
BBa_J329021	.	engineered_region	1204	1821	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_J329021	.	tag	1783	1814	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_J329021	.	engineered_tag	1822	1846	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_J329021	.	CDS	1204	1782	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_J329021	.	promoter	1992	2046	.	+	0	ID=BBa_R0062;Name=BBa_R0062;Parent=BBa_J329010
BBa_J329021	.	engineered_region	1992	2046	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_J329021	.	promoter	2011	2016	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_J329021	.	promoter	2033	2038	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_J329021	.	start_codon	2044	2044	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_J329021	.	non_covalent_binding_site	1992	2011	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_J329021	.	promoter	2977	3031	.	+	0	ID=BBa_R0062;Name=BBa_R0062
BBa_J329021	.	engineered_region	2977	3031	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_J329021	.	promoter	2996	3001	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_J329021	.	promoter	3018	3023	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_J329021	.	start_codon	3029	3029	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_J329021	.	non_covalent_binding_site	2977	2996	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_J329021	.	ribosome_entry_site	3040	3070	.	+	0	ID=BBa_J329999;Name=BBa_J329999
BBa_J329021	.	CDS	3077	3694	.	+	0	ID=BBa_C0061;Name=BBa_C0061
BBa_J329021	.	engineered_region	3077	3694	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_J329021	.	tag	3656	3687	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_J329021	.	engineered_tag	3695	3719	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_J329021	.	CDS	3077	3655	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_J329021	.	terminator	3728	3856	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_J329021	.	terminator	3816	3856	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_J329021	.	polyA_site	3843	3856	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J329021	.	engineered_region	3816	3856	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J329021	.	stem_loop	3823	3842	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J329021	.	stop_codon	3849	3849	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J329021	.	terminator	3728	3807	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_J329021	.	stem_loop	3739	3782	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J329021	.	engineered_region	3728	3807	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
>BBa_J329021
acctgtacgatcctacaggtgcttatgttaagtaattgtattcccagcgatacaatagtgtgacaaaaatccaatttat
tagaatcaaatgtcaatccattaccgttttaatgatatataacacgcaaaacttgcgacaaacaataggtaatactaga
gaaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtaga
agcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcattt
atcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgc
taatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaac
aatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttcccta
ttcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttattttt
acatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaa
tcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaa
aaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctg
ccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctag
tgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttg
tttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacc
tgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaatactagagagtaataagtcaagcgcac
tacaaatcccgttactagatgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggta
ttctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatga
gtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacct
acaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatag
tcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatt
tgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgattt
ttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtat
tgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagctta
ataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactggg
cctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctg
cgtttatatactagagacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaatactagag
aaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtg
atgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttat
ttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgaga
tacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttt
tcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaa
aggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatc
atggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactag
cagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatc
tgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacat
ggcatggatgaactatacaaaaggcctgctgcaaacgacgaaaactacgctttagtagcttaataatactagagccagg
catcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctact
agagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtttacgc
aagaaaatggtttgttatagtcgaataaatactagagagtaataagtcaagcgcactacaaatcccgttactagatgac
tataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatcaagtg
tttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaat
atatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaa
aagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgta
ggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaacacgctg
ttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttccttg
tcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatgaacag
tttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgta
gatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttg
tcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata