LOCUS BBa_J329030 4028 bp DNA linear 22-Nov-2024 DEFINITION QS System with BBa_J329998 Synthetic RBS + Const. RFP Expression ACCESSION BBa_J329030 VERSION BBa_J329030.1 FEATURES Location/Qualifiers misc_feature 1..20 /label=LuxR/HSL promoter 115..122 /label=-10 promoter 89..94 /label=-35 misc_feature 128..128 /label=start misc_feature 130..149 /label=Putative LuxR/HSL misc_feature 1..151 /label=BBa_R0063 promoter 1..151 /label=BBa_R0063 misc_feature 164..167 /label=conserved RBS 160..171 /label=BBa_B0034 misc_feature 178..179 /label=prefix misc_feature 351..351 /label=T misc_feature 669..669 /label=A CDS 178..927 /label=luxR misc_feature 178..933 /label=BBa_C0062 misc_feature 934..958 /label=Help:Barcodes CDS 178..933 /label=BBa_C0062 stem_loop 978..1021 /label=stem_loop misc_feature 967..1046 /label=BBa_B0010 terminator 967..1046 /label=BBa_B0010 stem_loop 1062..1081 /label=T7 TE misc_feature 1088..1088 /label=stop polyA_site 1082..1095 /label=polya misc_feature 1055..1095 /label=BBa_B0012 terminator 1055..1095 /label=BBa_B0012 terminator 967..1095 /label=BBa_B0015 misc_feature 1104..1123 /label=LuxR/HSL promoter 1123..1128 /label=-35 promoter 1145..1150 /label=-10 misc_feature 1156..1156 /label=start misc_feature 1104..1158 /label=BBa_R0062 promoter 1104..1158 /label=BBa_R0062 RBS 1167..1180 /label=BBa_J329998 misc_feature 1766..1797 /label=LVA CDS 1187..1765 /label=luxI misc_feature 1187..1804 /label=BBa_C0061 misc_feature 1805..1829 /label=Help:Barcodes CDS 1187..1804 /label=BBa_C0061 stem_loop 1849..1892 /label=stem_loop misc_feature 1838..1917 /label=BBa_B0010 terminator 1838..1917 /label=BBa_B0010 stem_loop 1933..1952 /label=T7 TE misc_feature 1959..1959 /label=stop polyA_site 1953..1966 /label=polya misc_feature 1926..1966 /label=BBa_B0012 terminator 1926..1966 /label=BBa_B0012 terminator 1838..1966 /label=BBa_B0015 misc_feature 1975..1994 /label=LuxR/HSL promoter 1994..1999 /label=-35 promoter 2016..2021 /label=-10 misc_feature 2027..2027 /label=start misc_feature 1975..2029 /label=BBa_R0062 promoter 1975..2029 /label=BBa_R0062 misc_feature 2042..2045 /label=conserved RBS 2038..2049 /label=BBa_B0034 misc_feature 2056..2775 /label=BBa_E0040 misc_feature 2770..2808 /label=LVA misc_feature 2809..2814 /label=Double Stop misc_feature 2056..2058 /label=Start misc_feature 2056..2814 /label=BBa_J04031 stem_loop 2834..2877 /label=stem_loop misc_feature 2823..2902 /label=BBa_B0010 terminator 2823..2902 /label=BBa_B0010 stem_loop 2918..2937 /label=T7 TE misc_feature 2944..2944 /label=stop polyA_site 2938..2951 /label=polya misc_feature 2911..2951 /label=BBa_B0012 terminator 2911..2951 /label=BBa_B0012 misc_feature 2038..2951 /label=BBa_I763020 CDS 2960..3047 /label=end of LacI coding region (inactive) misc_feature 2960..3159 /label=BBa_R0010 misc_feature 3048..3085 /label=CAP binding site promoter 3096..3101 /label=-35 promoter 3120..3125 /label=-10 misc_feature 3125..3159 /label=LacI binding site misc_feature 3132..3132 /label=start promoter 2960..3159 /label=BBa_R0010 misc_feature 3172..3175 /label=conserved RBS 3168..3179 /label=BBa_B0034 CDS 3186..3860 /label=mrfp1 misc_feature 3867..3891 /label=Help:Barcodes CDS 3186..3866 /label=BBa_E1010 stem_loop 3911..3954 /label=stem_loop misc_feature 3900..3979 /label=BBa_B0010 terminator 3900..3979 /label=BBa_B0010 stem_loop 3995..4014 /label=T7 TE misc_feature 4021..4021 /label=stop polyA_site 4015..4028 /label=polya misc_feature 3988..4028 /label=BBa_B0012 terminator 3988..4028 /label=BBa_B0012 misc_feature 2960..4028 /label=BBa_J04450 ORIGIN 1 acctgtacga tcctacaggt gcttatgtta agtaattgta ttcccagcga tacaatagtg 61 tgacaaaaat ccaatttatt agaatcaaat gtcaatccat taccgtttta atgatatata 121 acacgcaaaa cttgcgacaa acaataggta atactagaga aagaggagaa atactagatg 181 aaaaacataa atgccgacga cacatacaga ataattaata aaattaaagc ttgtagaagc 241 aataatgata ttaatcaatg cttatctgat atgactaaaa tggtacattg tgaatattat 301 ttactcgcga tcatttatcc tcattctatg gttaaatctg atatttcaat cctagataat 361 taccctaaaa aatggaggca atattatgat gacgctaatt taataaaata tgatcctata 421 gtagattatt ctaactccaa tcattcacca attaattgga atatatttga aaacaatgct 481 gtaaataaaa aatctccaaa tgtaattaaa gaagcgaaaa catcaggtct tatcactggg 541 tttagtttcc ctattcatac ggctaacaat ggcttcggaa tgcttagttt tgcacattca 601 gaaaaagaca actatataga tagtttattt ttacatgcgt gtatgaacat accattaatt 661 gttccttctc tagttgataa ttatcgaaaa ataaatatag caaataataa atcaaacaac 721 gatttaacca aaagagaaaa agaatgttta gcgtgggcat gcgaaggaaa aagctcttgg 781 gatatttcaa aaatattagg ttgcagtgag cgtactgtca ctttccattt aaccaatgcg 841 caaatgaaac tcaatacaac aaaccgctgc caaagtattt ctaaagcaat tttaacagga 901 gcaattgatt gcccatactt taaaaattaa taacactgat agtgctagtg tagatcacta 961 ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta 1021 tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc 1081 ctttctgcgt ttatatacta gagacctgta ggatcgtaca ggtttacgca agaaaatggt 1141 ttgttatagt cgaataaata ctagagggtc cacgaatatc tactagatga ctataatgat 1201 aaaaaaatcg gattttttgg caattccatc ggaggagtat aaaggtattc taagtcttcg 1261 ttatcaagtg tttaagcaaa gacttgagtg ggacttagtt gtagaaaata accttgaatc 1321 agatgagtat gataactcaa atgcagaata tatttatgct tgtgatgata ctgaaaatgt 1381 aagtggatgc tggcgtttat tacctacaac aggtgattat atgctgaaaa gtgtttttcc 1441 tgaattgctt ggtcaacaga gtgctcccaa agatcctaat atagtcgaat taagtcgttt 1501 tgctgtaggt aaaaatagct caaagataaa taactctgct agtgaaatta caatgaaact 1561 atttgaagct atatataaac acgctgttag tcaaggtatt acagaatatg taacagtaac 1621 atcaacagca atagagcgat ttttaaagcg tattaaagtt ccttgtcatc gtattggaga 1681 caaagaaatt catgtattag gtgatactaa atcggttgta ttgtctatgc ctattaatga 1741 acagtttaaa aaagcagtct taaatgctgc aaacgacgaa aactacgctt tagtagctta 1801 ataactctga tagtgctagt gtagatctct actagagcca ggcatcaaat aaaacgaaag 1861 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac 1921 tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttatatact agagacctgt 1981 aggatcgtac aggtttacgc aagaaaatgg tttgttatag tcgaataaat actagagaaa 2041 gaggagaaat actagatgcg taaaggagaa gaacttttca ctggagttgt cccaattctt 2101 gttgaattag atggtgatgt taatgggcac aaattttctg tcagtggaga gggtgaaggt 2161 gatgcaacat acggaaaact tacccttaaa tttatttgca ctactggaaa actacctgtt 2221 ccatggccaa cacttgtcac tactttcggt tatggtgttc aatgctttgc gagataccca 2281 gatcatatga aacagcatga ctttttcaag agtgccatgc ccgaaggtta tgtacaggaa 2341 agaactatat ttttcaaaga tgacgggaac tacaagacac gtgctgaagt caagtttgaa 2401 ggtgataccc ttgttaatag aatcgagtta aaaggtattg attttaaaga agatggaaac 2461 attcttggac acaaattgga atacaactat aactcacaca atgtatacat catggcagac 2521 aaacaaaaga atggaatcaa agttaacttc aaaattagac acaacattga agatggaagc 2581 gttcaactag cagaccatta tcaacaaaat actccaattg gcgatggccc tgtcctttta 2641 ccagacaacc attacctgtc cacacaatct gccctttcga aagatcccaa cgaaaagaga 2701 gaccacatgg tccttcttga gtttgtaaca gctgctggga ttacacatgg catggatgaa 2761 ctatacaaaa ggcctgctgc aaacgacgaa aactacgctt tagtagctta ataatactag 2821 agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg 2881 ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt 2941 ctgcgtttat atactagagc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat 3001 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt 3061 aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt 3121 atgttgtgtg gaattgtgag cggataacaa tttcacacat actagagaaa gaggagaaat 3181 actagatggc ttcctccgaa gacgttatca aagagttcat gcgtttcaaa gttcgtatgg 3241 aaggttccgt taacggtcac gagttcgaaa tcgaaggtga aggtgaaggt cgtccgtacg 3301 aaggtaccca gaccgctaaa ctgaaagtta ccaaaggtgg tccgctgccg ttcgcttggg 3361 acatcctgtc cccgcagttc cagtacggtt ccaaagctta cgttaaacac ccggctgaca 3421 tcccggacta cctgaaactg tccttcccgg aaggtttcaa atgggaacgt gttatgaact 3481 tcgaagacgg tggtgttgtt accgttaccc aggactcctc cctgcaagac ggtgagttca 3541 tctacaaagt taaactgcgt ggtaccaact tcccgtccga cggtccggtt atgcagaaaa 3601 aaaccatggg ttgggaagct tccaccgaac gtatgtaccc ggaagacggt gctctgaaag 3661 gtgaaatcaa aatgcgtctg aaactgaaag acggtggtca ctacgacgct gaagttaaaa 3721 ccacctacat ggctaaaaaa ccggttcagc tgccgggtgc ttacaaaacc gacatcaaac 3781 tggacatcac ctcccacaac gaagactaca ccatcgttga acagtacgaa cgtgctgaag 3841 gtcgtcactc caccggtgct taataacgct gatagtgcta gtgtagatcg ctactagagc 3901 caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg 3961 tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg 4021 cgtttata //