##gff-version 3
##sequence-region BBa_J329030 1 4028
BBa_J329030	.	promoter	1975	2029	.	+	0	ID=BBa_R0062;Name=BBa_R0062
BBa_J329030	.	start_codon	2027	2027	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_J329030	.	promoter	1994	1999	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_J329030	.	promoter	2016	2021	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_J329030	.	engineered_region	1975	2029	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_J329030	.	non_covalent_binding_site	1975	1994	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_J329030	.	engineered_region	2960	4028	.	+	0	ID=BBa_J04450;Name=BBa_J04450
BBa_J329030	.	terminator	3988	4028	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_J04450
BBa_J329030	.	polyA_site	4015	4028	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J329030	.	stem_loop	3995	4014	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J329030	.	stop_codon	4021	4021	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J329030	.	engineered_region	3988	4028	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J329030	.	ribosome_entry_site	3168	3179	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J04450
BBa_J329030	.	conserved_region	3172	3175	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J329030	.	CDS	3186	3866	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_J04450
BBa_J329030	.	engineered_tag	3867	3891	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_J329030	.	CDS	3186	3860	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_J329030	.	terminator	3900	3979	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_J04450
BBa_J329030	.	stem_loop	3911	3954	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J329030	.	engineered_region	3900	3979	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J329030	.	promoter	2960	3159	.	+	0	ID=BBa_R0010;Name=BBa_R0010;Parent=BBa_J04450
BBa_J329030	.	non_covalent_binding_site	3125	3159	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_J329030	.	promoter	3096	3101	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_J329030	.	CDS	2960	3047	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_J329030	.	start_codon	3132	3132	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_J329030	.	non_covalent_binding_site	3048	3085	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_J329030	.	promoter	3120	3125	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_J329030	.	engineered_region	2960	3159	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_J329030	.	CDS	178	933	.	+	0	ID=BBa_C0062;Name=BBa_C0062
BBa_J329030	.	CDS	178	927	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_J329030	.	point_mutation	669	669	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_J329030	.	point_mutation	351	351	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_J329030	.	engineered_tag	934	958	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_J329030	.	engineered_region	178	179	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_J329030	.	engineered_region	178	933	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_J329030	.	promoter	1104	1158	.	+	0	ID=BBa_R0062;Name=BBa_R0062
BBa_J329030	.	start_codon	1156	1156	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_J329030	.	promoter	1123	1128	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_J329030	.	promoter	1145	1150	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_J329030	.	engineered_region	1104	1158	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_J329030	.	non_covalent_binding_site	1104	1123	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_J329030	.	engineered_region	2038	2951	.	+	0	ID=BBa_I763020;Name=BBa_I763020
BBa_J329030	.	ribosome_entry_site	2038	2049	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I763020
BBa_J329030	.	conserved_region	2042	2045	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J329030	.	engineered_region	2056	2814	.	+	0	ID=BBa_J04031;Name=BBa_J04031;Parent=BBa_I763020
BBa_J329030	.	start_codon	2056	2058	.	+	0	ID=annotation1507246;Name=Start;Parent=BBa_J04031
BBa_J329030	.	tag	2770	2808	.	+	0	ID=annotation1507235;Name=LVA;Parent=BBa_J04031
BBa_J329030	.	engineered_region	2056	2775	.	+	0	ID=annotation1507202;Name=BBa_E0040;Parent=BBa_J04031
BBa_J329030	.	stop_codon	2809	2814	.	+	0	ID=annotation1507242;Name=Double Stop;Parent=BBa_J04031
BBa_J329030	.	terminator	2823	2902	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I763020
BBa_J329030	.	stem_loop	2834	2877	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J329030	.	engineered_region	2823	2902	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J329030	.	terminator	2911	2951	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I763020
BBa_J329030	.	polyA_site	2938	2951	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J329030	.	stem_loop	2918	2937	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J329030	.	stop_codon	2944	2944	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J329030	.	engineered_region	2911	2951	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J329030	.	ribosome_entry_site	160	171	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_J329030	.	conserved_region	164	167	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J329030	.	ribosome_entry_site	1167	1180	.	+	0	ID=BBa_J329998;Name=BBa_J329998
BBa_J329030	.	terminator	1838	1966	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_J329030	.	terminator	1926	1966	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_J329030	.	polyA_site	1953	1966	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J329030	.	stem_loop	1933	1952	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J329030	.	stop_codon	1959	1959	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J329030	.	engineered_region	1926	1966	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J329030	.	terminator	1838	1917	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_J329030	.	stem_loop	1849	1892	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J329030	.	engineered_region	1838	1917	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J329030	.	terminator	967	1095	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_J329030	.	terminator	1055	1095	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_J329030	.	polyA_site	1082	1095	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J329030	.	stem_loop	1062	1081	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J329030	.	stop_codon	1088	1088	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J329030	.	engineered_region	1055	1095	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J329030	.	terminator	967	1046	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_J329030	.	stem_loop	978	1021	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J329030	.	engineered_region	967	1046	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J329030	.	promoter	1	151	.	+	0	ID=BBa_R0063;Name=BBa_R0063
BBa_J329030	.	non_covalent_binding_site	1	20	.	+	0	ID=annotation2051;Name=LuxR/HSL;Parent=BBa_R0063
BBa_J329030	.	promoter	115	122	.	+	0	ID=annotation2052;Name=-10;Parent=BBa_R0063
BBa_J329030	.	engineered_region	1	151	.	+	0	ID=annotation7071;Name=BBa_R0063;Parent=BBa_R0063
BBa_J329030	.	start_codon	128	128	.	+	0	ID=annotation2054;Name=start;Parent=BBa_R0063
BBa_J329030	.	promoter	89	94	.	+	0	ID=annotation2053;Name=-35;Parent=BBa_R0063
BBa_J329030	.	non_covalent_binding_site	130	149	.	+	0	ID=annotation2055;Name=Putative LuxR/HSL;Parent=BBa_R0063
BBa_J329030	.	CDS	1187	1804	.	+	0	ID=BBa_C0061;Name=BBa_C0061
BBa_J329030	.	CDS	1187	1765	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_J329030	.	engineered_region	1187	1804	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_J329030	.	engineered_tag	1805	1829	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_J329030	.	tag	1766	1797	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
>BBa_J329030
acctgtacgatcctacaggtgcttatgttaagtaattgtattcccagcgatacaatagtgtgacaaaaatccaatttat
tagaatcaaatgtcaatccattaccgttttaatgatatataacacgcaaaacttgcgacaaacaataggtaatactaga
gaaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtaga
agcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcattt
atcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgc
taatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaac
aatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttcccta
ttcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttattttt
acatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaa
tcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaa
aaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctg
ccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctag
tgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttg
tttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacc
tgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaatactagagggtccacgaatatctacta
gatgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttat
caagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatg
cagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatat
gctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgtttt
gctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaac
acgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagt
tccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaat
gaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgct
agtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgt
tgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagaga
cctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaatactagagaaagaggagaaatacta
gatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaa
ttttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaac
tacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaa
acagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaac
tacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaag
aagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaa
gaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaa
aatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatc
ccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactata
caaaaggcctgctgcaaacgacgaaaactacgctttagtagcttaataatactagagccaggcatcaaataaaacgaaa
ggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctca
ccttcgggtgggcctttctgcgtttatatactagagcaatacgcaaaccgcctctccccgcgcgttggccgattcatta
atgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcat
taggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacat
actagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatg
gaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgcta
aactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagc
ttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatg
aacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaac
tgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtat
gtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaa
gttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacct
cccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctg
atagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgtt
ttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata