##gff-version 3
##sequence-region BBa_J329055 1 1177
BBa_J329055	.	engineered_region	1	220	.	+	0	ID=BBa_J04500;Name=BBa_J04500
BBa_J329055	.	promoter	1	200	.	+	0	ID=BBa_R0010;Name=BBa_R0010;Parent=BBa_J04500
BBa_J329055	.	non_covalent_binding_site	89	126	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_J329055	.	promoter	137	142	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_J329055	.	start_codon	173	173	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_J329055	.	CDS	1	88	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_J329055	.	promoter	161	166	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_J329055	.	non_covalent_binding_site	166	200	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_J329055	.	engineered_region	1	200	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_J329055	.	ribosome_entry_site	209	220	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J04500
BBa_J329055	.	conserved_region	213	216	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J329055	.	CDS	227	1015	.	+	0	ID=BBa_C0060;Name=BBa_C0060
BBa_J329055	.	engineered_region	227	1015	.	+	0	ID=annotation7037;Name=BBa_C0060;Parent=BBa_C0060
BBa_J329055	.	tag	977	1009	.	+	0	ID=annotation1756;Name=LVA;Parent=BBa_C0060
BBa_J329055	.	start_codon	227	229	.	+	0	ID=annotation1754;Name=start;Parent=BBa_C0060
BBa_J329055	.	stop_codon	1010	1015	.	+	0	ID=annotation1755;Name=2;Parent=BBa_C0060
BBa_J329055	.	engineered_tag	1016	1040	.	+	0	ID=annotation2213987;Name=Help:Barcodes;Parent=BBa_C0060
BBa_J329055	.	CDS	227	976	.	+	0	ID=annotation1757;Name=aiiA;Parent=BBa_C0060
BBa_J329055	.	terminator	1049	1177	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_J329055	.	terminator	1137	1177	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_J329055	.	polyA_site	1164	1177	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J329055	.	engineered_region	1137	1177	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J329055	.	stem_loop	1144	1163	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J329055	.	stop_codon	1170	1170	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J329055	.	terminator	1049	1128	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_J329055	.	engineered_region	1049	1128	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J329055	.	stem_loop	1060	1103	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
>BBa_J329055
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagc
gggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggct
cgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatgacagtaaa
gaagctttatttcgtcccagcaggtcgttgtatgttggatcattcgtctgttaatagtacattaacaccaggagaatta
ttagacttaccggtttggtgttatcttttggagactgaagaaggacctattttagtagatacaggtatgccagaaagtg
cagttaataatgaaggtctttttaacggtacatttgtcgaagggcaggttttaccgaaaatgactgaagaagatagaat
cgtgaatattttaaaacgggttggttatgagccggaagaccttctttatattattagttctcacttgcattttgatcat
gcaggaggaaatggcgcttttataaatacaccaatcattgtacagcgtgctgaatatgaggcggcgcagcatagcgaag
aatatttgaaagaatgtatattgccgaatttaaactacaaaatcattgaaggtgattatgaagtcgtaccaggagttca
attattgcatacaccaggccatactccagggcatcaatcgctattaattgagacagaaaaatccggtcctgtattatta
acgattgatgcatcgtatacgaaagagaattttgaaaatgaagtgccatttgcgggatttgattcagaattagctttat
cttcaattaaacgtttaaaagaagtggtgatgaaagagaagccgattgttttctttggacatgatatagagcaggaaag
gggatgtaaagtgttccctgaatatatagctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgc
tagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctg
ttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata