LOCUS       BBa_J329060             2089 bp    DNA     linear       22-Nov-2024
DEFINITION  Strong AiiA Expression with RFP
ACCESSION   BBa_J329060
VERSION     BBa_J329060.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23100
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     misc_feature    1..55
                     /label=BBa_K880005
     misc_feature    62..64
                     /label=start
     misc_feature    845..850
                     /label=2
     misc_feature    812..844
                     /label=LVA
     CDS             62..811
                     /label=aiiA
     misc_feature    62..850
                     /label=BBa_C0060
     misc_feature    851..875
                     /label=Help:Barcodes
     CDS             62..850
                     /label=BBa_C0060
     stem_loop       895..938
                     /label=stem_loop
     misc_feature    884..963
                     /label=BBa_B0010
     terminator      884..963
                     /label=BBa_B0010
     stem_loop       979..998
                     /label=T7 TE
     misc_feature    1005..1005
                     /label=stop
     polyA_site      999..1012
                     /label=polya
     misc_feature    972..1012
                     /label=BBa_B0012
     terminator      972..1012
                     /label=BBa_B0012
     terminator      884..1012
                     /label=BBa_B0015
     misc_feature    1..1012
                     /label=BBa_J329050
     CDS             1021..1108
                     /label=end of LacI coding region (inactive)
     misc_feature    1021..1220
                     /label=BBa_R0010
     misc_feature    1109..1146
                     /label=CAP binding site
     promoter        1157..1162
                     /label=-35
     promoter        1181..1186
                     /label=-10
     misc_feature    1186..1220
                     /label=LacI binding site
     misc_feature    1193..1193
                     /label=start
     promoter        1021..1220
                     /label=BBa_R0010
     misc_feature    1233..1236
                     /label=conserved
     RBS             1229..1240
                     /label=BBa_B0034
     CDS             1247..1921
                     /label=mrfp1
     misc_feature    1928..1952
                     /label=Help:Barcodes
     CDS             1247..1927
                     /label=BBa_E1010
     stem_loop       1972..2015
                     /label=stem_loop
     misc_feature    1961..2040
                     /label=BBa_B0010
     terminator      1961..2040
                     /label=BBa_B0010
     stem_loop       2056..2075
                     /label=T7 TE
     misc_feature    2082..2082
                     /label=stop
     polyA_site      2076..2089
                     /label=polya
     misc_feature    2049..2089
                     /label=BBa_B0012
     terminator      2049..2089
                     /label=BBa_B0012
     misc_feature    1021..2089
                     /label=BBa_J04450
ORIGIN
        1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gagaaagagg agaaatacta
       61 gatgacagta aagaagcttt atttcgtccc agcaggtcgt tgtatgttgg atcattcgtc
      121 tgttaatagt acattaacac caggagaatt attagactta ccggtttggt gttatctttt
      181 ggagactgaa gaaggaccta ttttagtaga tacaggtatg ccagaaagtg cagttaataa
      241 tgaaggtctt tttaacggta catttgtcga agggcaggtt ttaccgaaaa tgactgaaga
      301 agatagaatc gtgaatattt taaaacgggt tggttatgag ccggaagacc ttctttatat
      361 tattagttct cacttgcatt ttgatcatgc aggaggaaat ggcgctttta taaatacacc
      421 aatcattgta cagcgtgctg aatatgaggc ggcgcagcat agcgaagaat atttgaaaga
      481 atgtatattg ccgaatttaa actacaaaat cattgaaggt gattatgaag tcgtaccagg
      541 agttcaatta ttgcatacac caggccatac tccagggcat caatcgctat taattgagac
      601 agaaaaatcc ggtcctgtat tattaacgat tgatgcatcg tatacgaaag agaattttga
      661 aaatgaagtg ccatttgcgg gatttgattc agaattagct ttatcttcaa ttaaacgttt
      721 aaaagaagtg gtgatgaaag agaagccgat tgttttcttt ggacatgata tagagcagga
      781 aaggggatgt aaagtgttcc ctgaatatat agctgcaaac gacgaaaact acgctttagt
      841 agcttaataa cgctgatagt gctagtgtag atcgctacta gagccaggca tcaaataaaa
      901 cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct
      961 ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta tatactagag
     1021 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
     1081 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
     1141 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
     1201 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgg cttcctccga
     1261 agacgttatc aaagagttca tgcgtttcaa agttcgtatg gaaggttccg ttaacggtca
     1321 cgagttcgaa atcgaaggtg aaggtgaagg tcgtccgtac gaaggtaccc agaccgctaa
     1381 actgaaagtt accaaaggtg gtccgctgcc gttcgcttgg gacatcctgt ccccgcagtt
     1441 ccagtacggt tccaaagctt acgttaaaca cccggctgac atcccggact acctgaaact
     1501 gtccttcccg gaaggtttca aatgggaacg tgttatgaac ttcgaagacg gtggtgttgt
     1561 taccgttacc caggactcct ccctgcaaga cggtgagttc atctacaaag ttaaactgcg
     1621 tggtaccaac ttcccgtccg acggtccggt tatgcagaaa aaaaccatgg gttgggaagc
     1681 ttccaccgaa cgtatgtacc cggaagacgg tgctctgaaa ggtgaaatca aaatgcgtct
     1741 gaaactgaaa gacggtggtc actacgacgc tgaagttaaa accacctaca tggctaaaaa
     1801 accggttcag ctgccgggtg cttacaaaac cgacatcaaa ctggacatca cctcccacaa
     1861 cgaagactac accatcgttg aacagtacga acgtgctgaa ggtcgtcact ccaccggtgc
     1921 ttaataacgc tgatagtgct agtgtagatc gctactagag ccaggcatca aataaaacga
     1981 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc
     2041 tactagagtc acactggctc accttcgggt gggcctttct gcgtttata
//