BBa_J331011
type: ComponentDefinition
persistent identity: BBa J331011
display id: BBa_J331011
version: 1
was generated by: iGEM to SBOL conversion
description: Water Purification from Lead
created: 2016-12-03T12:00:00Z
modified: 2016-12-03T03:34:21Z
mutable provenance: Anderson promoter comes from part BBa_J23119. The RBS comes from part BBa_0030. CBP4 is naturally found in Nicotina tabacum, but it also comes from part BBa_J331007. Terminator comes from part BBa_B0014. T7 promoter comes from part BBa_I719005. GST is naturally found in S. japonicum, but it also comes from part BBa_T2025. CRS5 is a metallothionein found in S. serevisiae, but also comes from part BBa_ J331009. The linking sequence that will link CRS5 and GST comes from part BBa_J331010.
owned by: james
owned by: myers
mutable description: This main purpose of this composite is to purify water from lead, so that it is cleaner for its use. This gene can be inserted into E.coli so that the bacteria can be placed in a tank filled with contaminated water and purify the water from lead.
discontinued: false
dominant: false
group u list: _1979_
m user id: 24251
owner id: 24251
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: None
star: false
creator: Gilles Yvan Buck
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_J23119
component: BBa_J331007
component: BBa_B0030
component: BBa_B0014
component: BBa_J331010
component: BBa_T2025
component: BBa_I719005
component: BBa_J331009
sequence annotation: BBa_J23119
sequence annotation: BBa_B0030
sequence annotation: BBa_J331007
sequence annotation: BBa_B0014
sequence annotation: BBa_I719005
sequence annotation: BBa_T2025
sequence annotation: BBa_J331010
sequence annotation: BBa_J331009
sequence: https://synbiohub.org/public/igem/BBa_J331011_sequence/1

BBa_J23119
type: Component
persistent identity: component2531686
display id: component2531686
version: 1
top level: BBa_J331011
definition: BBa_J23119
access: public

BBa_J331007
type: Component
persistent identity: component2531690
display id: component2531690
version: 1
top level: BBa_J331011
definition: BBa_J331007
access: public

BBa_B0030
type: Component
persistent identity: component2531688
display id: component2531688
version: 1
top level: BBa_J331011
definition: BBa_B0030
access: public

BBa_B0014
type: Component
persistent identity: component2531700
display id: component2531700
version: 1
top level: BBa_J331011
definition: BBa_B0014
access: public

BBa_B0030
type: Component
persistent identity: component2531692
display id: component2531692
version: 1
top level: BBa_J331011
definition: BBa_B0030
access: public

BBa_J331010
type: Component
persistent identity: component2531707
display id: component2531707
version: 1
top level: BBa_J331011
definition: BBa_J331010
access: public

BBa_B0030
type: Component
persistent identity: component2531703
display id: component2531703
version: 1
top level: BBa_J331011
definition: BBa_B0030
access: public

BBa_T2025
type: Component
persistent identity: component2531706
display id: component2531706
version: 1
top level: BBa_J331011
definition: BBa_T2025
access: public

BBa_B0030
type: Component
persistent identity: component2531710
display id: component2531710
version: 1
top level: BBa_J331011
definition: BBa_B0030
access: public

BBa_I719005
type: Component
persistent identity: component2531701
display id: component2531701
version: 1
top level: BBa_J331011
definition: pT7
access: public

BBa_J331009
type: Component
persistent identity: component2531708
display id: component2531708
version: 1
top level: BBa_J331011
definition: BBa_J331009
access: public

BBa_B0014
type: Component
persistent identity: component2531718
display id: component2531718
version: 1
top level: BBa_J331011
definition: BBa_B0014
access: public

BBa_J23119
type: SequenceAnnotation
persistent identity: annotation2531686
display id: annotation2531686
version: 1
top level: BBa_J331011
location: https://synbiohub.org/public/igem/BBa_J331011/annotation2531686/range2531686/1
component: BBa_J23119

https://synbiohub.org/public/igem/BBa_J331011/annotation2531686/range2531686/1
type: Range
persistent identity: range2531686
display id: range2531686
version: 1
top level: BBa_J331011
direction: direction/reverse
start: 1
end: 35
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2531688
display id: annotation2531688
version: 1
top level: BBa_J331011
location: https://synbiohub.org/public/igem/BBa_J331011/annotation2531688/range2531688/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_J331011/annotation2531688/range2531688/1
type: Range
persistent identity: range2531688
display id: range2531688
version: 1
top level: BBa_J331011
direction: direction/forward
start: 44
end: 58
orientation: inline

BBa_J331007
type: SequenceAnnotation
persistent identity: annotation2531690
display id: annotation2531690
version: 1
top level: BBa_J331011
location: https://synbiohub.org/public/igem/BBa_J331011/annotation2531690/range2531690/1
component: BBa_J331007

https://synbiohub.org/public/igem/BBa_J331011/annotation2531690/range2531690/1
type: Range
persistent identity: range2531690
display id: range2531690
version: 1
top level: BBa_J331011
direction: direction/reverse
start: 65
end: 577
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2531692
display id: annotation2531692
version: 1
top level: BBa_J331011
location: https://synbiohub.org/public/igem/BBa_J331011/annotation2531692/range2531692/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_J331011/annotation2531692/range2531692/1
type: Range
persistent identity: range2531692
display id: range2531692
version: 1
top level: BBa_J331011
direction: direction/forward
start: 586
end: 600
orientation: inline

BBa_B0014
type: SequenceAnnotation
persistent identity: annotation2531700
display id: annotation2531700
version: 1
top level: BBa_J331011
location: https://synbiohub.org/public/igem/BBa_J331011/annotation2531700/range2531700/1
component: BBa_B0014

https://synbiohub.org/public/igem/BBa_J331011/annotation2531700/range2531700/1
type: Range
persistent identity: range2531700
display id: range2531700
version: 1
top level: BBa_J331011
direction: direction/reverse
start: 609
end: 703
orientation: inline

BBa_I719005
type: SequenceAnnotation
persistent identity: annotation2531701
display id: annotation2531701
version: 1
top level: BBa_J331011
location: https://synbiohub.org/public/igem/BBa_J331011/annotation2531701/range2531701/1
component: BBa_I719005

https://synbiohub.org/public/igem/BBa_J331011/annotation2531701/range2531701/1
type: Range
persistent identity: range2531701
display id: range2531701
version: 1
top level: BBa_J331011
direction: direction/reverse
start: 712
end: 734
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2531703
display id: annotation2531703
version: 1
top level: BBa_J331011
location: https://synbiohub.org/public/igem/BBa_J331011/annotation2531703/range2531703/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_J331011/annotation2531703/range2531703/1
type: Range
persistent identity: range2531703
display id: range2531703
version: 1
top level: BBa_J331011
direction: direction/forward
start: 743
end: 757
orientation: inline

BBa_T2025
type: SequenceAnnotation
persistent identity: annotation2531706
display id: annotation2531706
version: 1
top level: BBa_J331011
location: https://synbiohub.org/public/igem/BBa_J331011/annotation2531706/range2531706/1
component: BBa_T2025

https://synbiohub.org/public/igem/BBa_J331011/annotation2531706/range2531706/1
type: Range
persistent identity: range2531706
display id: range2531706
version: 1
top level: BBa_J331011
direction: direction/reverse
start: 766
end: 1422
orientation: inline

BBa_J331010
type: SequenceAnnotation
persistent identity: annotation2531707
display id: annotation2531707
version: 1
top level: BBa_J331011
location: https://synbiohub.org/public/igem/BBa_J331011/annotation2531707/range2531707/1
component: BBa_J331010

https://synbiohub.org/public/igem/BBa_J331011/annotation2531707/range2531707/1
type: Range
persistent identity: range2531707
display id: range2531707
version: 1
top level: BBa_J331011
direction: direction/reverse
start: 1431
end: 1462
orientation: inline

BBa_J331009
type: SequenceAnnotation
persistent identity: annotation2531708
display id: annotation2531708
version: 1
top level: BBa_J331011
location: https://synbiohub.org/public/igem/BBa_J331011/annotation2531708/range2531708/1
component: BBa_J331009

https://synbiohub.org/public/igem/BBa_J331011/annotation2531708/range2531708/1
type: Range
persistent identity: range2531708
display id: range2531708
version: 1
top level: BBa_J331011
direction: direction/reverse
start: 1469
end: 1678
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2531710
display id: annotation2531710
version: 1
top level: BBa_J331011
location: https://synbiohub.org/public/igem/BBa_J331011/annotation2531710/range2531710/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_J331011/annotation2531710/range2531710/1
type: Range
persistent identity: range2531710
display id: range2531710
version: 1
top level: BBa_J331011
direction: direction/forward
start: 1687
end: 1701
orientation: inline

BBa_B0014
type: SequenceAnnotation
persistent identity: annotation2531718
display id: annotation2531718
version: 1
top level: BBa_J331011
location: https://synbiohub.org/public/igem/BBa_J331011/annotation2531718/range2531718/1
component: BBa_B0014

https://synbiohub.org/public/igem/BBa_J331011/annotation2531718/range2531718/1
type: Range
persistent identity: range2531718
display id: range2531718
version: 1
top level: BBa_J331011
direction: direction/reverse
start: 1710
end: 1804
orientation: inline

BBa_B0030
type: ComponentDefinition
persistent identity: BBa B0030
display id: BBa_B0030
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.1 (strong) -- modified from R. Weiss
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _44_46_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: RBS-1\Strong
sequence annotation: BBa_B0030
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0030_sequence/1

RBS-1\Strong
type: SequenceAnnotation
persistent identity: annotation1701
display id: annotation1701
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
type: Range
persistent identity: range1701
display id: range1701
version: 1
top level: BBa_B0030
direction: direction/forward
start: 1
end: 15
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation7025
display id: annotation7025
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
type: Range
persistent identity: range7025
display id: range7025
version: 1
top level: BBa_B0030
direction: direction/forward
start: 1
end: 15
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1702
display id: annotation1702
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
type: Range
persistent identity: range1702
display id: range1702
version: 1
top level: BBa_B0030
direction: direction/forward
start: 8
end: 12
orientation: inline

BBa_B0014
type: ComponentDefinition
persistent identity: BBa B0014
display id: BBa_B0014
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0012-B0011)
created: 2003-07-15T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0012 and BBa_B0011
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
component: BBa_B0011
component: BBa_B0012
sequence annotation: BBa_B0012
sequence annotation: BBa_B0011
sequence: https://synbiohub.org/public/igem/BBa_B0014_sequence/1

BBa_B0011
type: Component
persistent identity: component939311
display id: component939311
version: 1
top level: BBa_B0014
definition: BBa_B0011
access: public

BBa_B0012
type: Component
persistent identity: component939303
display id: component939303
version: 1
top level: BBa_B0014
definition: BBa_B0012
access: public

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation939303
display id: annotation939303
version: 1
top level: BBa_B0014
location: https://synbiohub.org/public/igem/BBa_B0014/annotation939303/range939303/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0014/annotation939303/range939303/1
type: Range
persistent identity: range939303
display id: range939303
version: 1
top level: BBa_B0014
direction: direction/forward
start: 1
end: 41
orientation: inline

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation939311
display id: annotation939311
version: 1
top level: BBa_B0014
location: https://synbiohub.org/public/igem/BBa_B0014/annotation939311/range939311/1
component: BBa_B0011

https://synbiohub.org/public/igem/BBa_B0014/annotation939311/range939311/1
type: Range
persistent identity: range939311
display id: range939311
version: 1
top level: BBa_B0014
direction: direction/forward
start: 50
end: 95
orientation: inline

pT7
type: ComponentDefinition
persistent identity: BBa I719005
display id: BBa_I719005
version: 1
was generated by: iGEM to SBOL conversion
description: T7 Promoter
created: 2007-10-23T11:00:00Z
modified: 2015-08-31T04:07:53Z
mutable provenance: ---
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Just a T7 Promoter
discontinued: false
dominant: false
experience: experience/Works
group u list: _128_
m user id: 0
owner id: 2097
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: None
star: true
creator: Imperial 2007
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence: https://synbiohub.org/public/igem/BBa_I719005_sequence/1

BBa_J23119
type: ComponentDefinition
persistent identity: BBa J23119
display id: BBa_J23119
version: 1
was generated by: iGEM to SBOL conversion
description: constitutive promoter family member
created: 2006-08-23T11:00:00Z
modified: 2015-08-31T04:08:40Z
mutable provenance: Overlap extension of synthetic oligonucleotides
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Later
discontinued: false
dominant: true
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence: https://synbiohub.org/public/igem/BBa_J23119_sequence/1

BBa_J331007
type: ComponentDefinition
persistent identity: BBa J331007
display id: BBa_J331007
version: 1
was generated by: iGEM to SBOL conversion
description: CBP4
created: 2016-12-03T12:00:00Z
modified: 2016-12-03T10:23:32Z
mutable provenance: It comes from a genomic sequence. http://www.yeastgenome.org/locus/S000003406/sequence
owned by: james
owned by: myers
mutable description: Mitochondrial protein required for gathering of cytochrome bc1 complex; interfaces with the Cbp3p-Cbp6p complex and recently integrated cytochrome b (Cobp) to advance get together of Cobp into the cytochrome bc1 complex. It was used by the 2014 Cornell Project as a part to remove lead from water.
discontinued: false
dominant: false
experience: experience/None
group u list: _1979_
m user id: 24251
owner id: 24251
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: None
star: false
creator: Gilles Yvan Buck
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence: https://synbiohub.org/public/igem/BBa_J331007_sequence/1

BBa_J331010
type: ComponentDefinition
persistent identity: BBa J331010
display id: BBa_J331010
version: 1
was generated by: iGEM to SBOL conversion
description: Linking Sequence for Metallothionein (CRS5) and Glutathione S-Transferase (GST)
created: 2016-12-03T12:00:00Z
modified: 2016-12-03T02:29:14Z
mutable provenance: It comes from a genomic sequence.
owned by: james
owned by: myers
mutable description: This is a linking sequence used to link two coding regions (CRS5 and GST) without any RBS. These two are used as part of the process of removing lead (Pb) from water.
discontinued: false
dominant: false
group u list: _1979_
m user id: 24251
owner id: 24251
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: None
star: false
creator: Gilles Yvan Buck
type: DnaRegion
role: partType/Other
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_J331010_sequence/1

BBa_T2025
type: ComponentDefinition
persistent identity: BBa T2025
display id: BBa_T2025
version: 1
was generated by: iGEM to SBOL conversion
description: Glutathione S-transferase internal domain
created: 2009-09-16T11:00:00Z
modified: 2015-05-08T01:14:52Z
mutable provenance: This is a synthetic sequence.
owned by: james
owned by: myers
mutable description: This is a GST internal domain designed to be between protein domains. GST domains are a type of affinity domain that can be used to purify and/or measure the quantity of proteins.
discontinued: false
dominant: false
group u list: _41_
m user id: 0
owner id: 126
owning group id: 162
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Rare codons in common model organisms were avoided along with restriction sites used in various assembly standards.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: GST
sequence: https://synbiohub.org/public/igem/BBa_T2025_sequence/1

GST
type: SequenceAnnotation
persistent identity: annotation2021346
display id: annotation2021346
version: 1
top level: BBa_T2025
location: https://synbiohub.org/public/igem/BBa_T2025/annotation2021346/range2021346/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_T2025/annotation2021346/range2021346/1
type: Range
persistent identity: range2021346
display id: range2021346
version: 1
top level: BBa_T2025
direction: direction/forward
start: 1
end: 657
orientation: inline

BBa_J331009
type: ComponentDefinition
persistent identity: BBa J331009
display id: BBa_J331009
version: 1
was generated by: iGEM to SBOL conversion
description: CSR5 (Metallothionein)
created: 2016-12-03T12:00:00Z
modified: 2016-12-03T11:21:39Z
mutable provenance: Metallothionein. Genomic Sequence
owned by: james
owned by: myers
mutable description: This coding region codes for the removal of heavy metals and the purification of substances. It can be used for the removal of lead, mercury, nickel, and others when combined with other biobricks to create a composite part. It was used by Cornell in 2014 to purify water from heavy metals.
discontinued: false
dominant: false
group u list: _1979_
m user id: 24251
owner id: 24251
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: None
star: false
creator: Gilles Yvan Buck
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_J331009_sequence/1

BBa_B0011
type: ComponentDefinition
persistent identity: BBa B0011
display id: BBa_B0011
version: 1
was generated by: iGEM to SBOL conversion
description: LuxICDABEG (+/-)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from luxICDABEG operon terminator of Vibrio fischeri <genbank>AF170104</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Bidirectional transcriptional terminator consisting of a 22 bp stem-loop.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: In the naturally-occuring sequence there is a mismatch in the stem of the stem loop. This can be corrected via an A->G mutation (at position 40 -- sequence coordinate/not MFOLD coordinate). The above sequence does not reflect this mutation (but the MFOLD image does). This terminator's location cannot be found using some inverted repeat detectors like PALINDROME because it is too short and contains a mismatch. This one was found with the help of Tom Knight. It lies between two coding regions that point towards eachother.
star: true
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0011
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0011_sequence/1

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation7019
display id: annotation7019
version: 1
top level: BBa_B0011
location: https://synbiohub.org/public/igem/BBa_B0011/annotation7019/range7019/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0011/annotation7019/range7019/1
type: Range
persistent identity: range7019
display id: range7019
version: 1
top level: BBa_B0011
direction: direction/forward
start: 1
end: 46
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation1683
display id: annotation1683
version: 1
top level: BBa_B0011
location: https://synbiohub.org/public/igem/BBa_B0011/annotation1683/range1683/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0011/annotation1683/range1683/1
type: Range
persistent identity: range1683
display id: range1683
version: 1
top level: BBa_B0011
direction: direction/forward
start: 13
end: 35
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

https://synbiohub.org/public/igem/BBa_J331011_sequence/1
type: Sequence
persistent identity: BBa J331011 sequence
display id: BBa_J331011_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacagctagctcagtcctaggtataatgctagctactagagattaaagaggagaaatactagatgcagtgcgcgataactcctagagaggcagtaatagcaaaacaaagacagtataagcattatttgggtatggaaagaccattgtgggtacgttggttgaaagtctatgcaattggtggcgctattattggaagcgggtttctgcttttcaagtacacaacgccaacagatcaacagttaatcagccagctttccccagaattacgtttacaatatgagagggagaaaaaactacgtcagtcggagcagcaggctttgatgaaaattgttaaggaaacttctcaaagtgacgatccaatttggaagacgggcccccttcaatcaccttgggaaaggaacggtgataacgttcaaagcagagatcactttgctaaggtgagggcggaggaggtgcaaaaagaagagttggctaggatcagaaatgaactgtctcagctaagatctgaaacggaggagaaaacaaaggaaatagtccaggataagcaggttaaaagctggtggcgcttctggtagtactagagattaaagaggagaaatactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattttactagagtaatacgactcactatagggagatactagagattaaagaggagaaatactagagtccccgattctgggttattggaaaatcaagggtcttgtccagccgacccgccttctcctggagtatcttgaggaaaaatatgaggaacacctttacgaacgtgacgagggcgataaatggcgtaacaaaaagttcgagcttggcctcgaatttccgaatcttccgtactacattgacggcgacgtcaaactgacccagagcatggcgattatccgttacatcgcggataaacataacatgctcggcggttgtccgaaagagcgcgccgaaatttccatgcttgaaggtgccgtcctggatattcgttacggtgtgtcccgtattgcctacagcaaagactttgaaacccttaaagtggattttctctcgaaacttccggaaatgcttaaaatgttcgaagatcgtctgtgtcacaagacctacctcaacggcgaccatgtgacccacccggacttcatgctttatgacgcgcttgacgtggtgctctacatggacccgatgtgcctggacgcgttcccgaaacttgtgtgctttaaaaagcgcattgaagcgatcccgcagatcgacaagtaccttaagtccagcaagtatatcgcgtggccgcttcagggttggcaagccaccttcggtggcggtgatcacccgccgaaatccgactactagagatcggatctggttccgcgtggatccattcatatactagatgactgtaaaaatatgtgactgttaaggcgaatgttgtaaggactcttgtcattgtgggagcacctgccttccaagctgttctggcggtgaaaagtgcaaatgtgatcacagcaccggaagccctcaatgtaagagttgtggtgaaaaatgcaaatgcgaaaccacgtgcacttgtgaaaagagtaaatgcaattgtgaaaaatgttagtactagagattaaagaggagaaatactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0030_sequence/1
type: Sequence
persistent identity: BBa B0030 sequence
display id: BBa_B0030_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attaaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0014_sequence/1
type: Sequence
persistent identity: BBa B0014 sequence
display id: BBa_B0014_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I719005_sequence/1
type: Sequence
persistent identity: BBa I719005 sequence
display id: BBa_I719005_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taatacgactcactatagggaga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23119_sequence/1
type: Sequence
persistent identity: BBa J23119 sequence
display id: BBa_J23119_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacagctagctcagtcctaggtataatgctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J331007_sequence/1
type: Sequence
persistent identity: BBa J331007 sequence
display id: BBa_J331007_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgcagtgcgcgataactcctagagaggcagtaatagcaaaacaaagacagtataagcattatttgggtatggaaagaccattgtgggtacgttggttgaaagtctatgcaattggtggcgctattattggaagcgggtttctgcttttcaagtacacaacgccaacagatcaacagttaatcagccagctttccccagaattacgtttacaatatgagagggagaaaaaactacgtcagtcggagcagcaggctttgatgaaaattgttaaggaaacttctcaaagtgacgatccaatttggaagacgggcccccttcaatcaccttgggaaaggaacggtgataacgttcaaagcagagatcactttgctaaggtgagggcggaggaggtgcaaaaagaagagttggctaggatcagaaatgaactgtctcagctaagatctgaaacggaggagaaaacaaaggaaatagtccaggataagcaggttaaaagctggtggcgcttctggtag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J331009_sequence/1
type: Sequence
persistent identity: BBa J331009 sequence
display id: BBa_J331009_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgactgtaaaaatatgtgactgttaaggcgaatgttgtaaggactcttgtcattgtgggagcacctgccttccaagctgttctggcggtgaaaagtgcaaatgtgatcacagcaccggaagccctcaatgtaagagttgtggtgaaaaatgcaaatgcgaaaccacgtgcacttgtgaaaagagtaaatgcaattgtgaaaaatgttag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J331010_sequence/1
type: Sequence
persistent identity: BBa J331010 sequence
display id: BBa_J331010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atcggatctggttccgcgtggatccattcata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_T2025_sequence/1
type: Sequence
persistent identity: BBa T2025 sequence
display id: BBa_T2025_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccccgattctgggttattggaaaatcaagggtcttgtccagccgacccgccttctcctggagtatcttgaggaaaaatatgaggaacacctttacgaacgtgacgagggcgataaatggcgtaacaaaaagttcgagcttggcctcgaatttccgaatcttccgtactacattgacggcgacgtcaaactgacccagagcatggcgattatccgttacatcgcggataaacataacatgctcggcggttgtccgaaagagcgcgccgaaatttccatgcttgaaggtgccgtcctggatattcgttacggtgtgtcccgtattgcctacagcaaagactttgaaacccttaaagtggattttctctcgaaacttccggaaatgcttaaaatgttcgaagatcgtctgtgtcacaagacctacctcaacggcgaccatgtgacccacccggacttcatgctttatgacgcgcttgacgtggtgctctacatggacccgatgtgcctggacgcgttcccgaaacttgtgtgctttaaaaagcgcattgaagcgatcccgcagatcgacaagtaccttaagtccagcaagtatatcgcgtggccgcttcagggttggcaagccaccttcggtggcggtgatcacccgccgaaatccgac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0011_sequence/1
type: Sequence
persistent identity: BBa B0011 sequence
display id: BBa_B0011_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: agagaatataaaaagccagattattaatccggcttttttattattt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z