Anderson promoter comes from part BBa_J23119. The RBS comes from part BBa_0030. CBP4 is naturally found in Nicotina tabacum, but it also comes from part BBa_J331007. Terminator comes from part BBa_B0014. T7 promoter comes from part BBa_I719005. GST is naturally found in S. japonicum, but it also comes from part BBa_T2025. CRS5 is a metallothionein found in S. serevisiae, but also comes from part BBa_ J331009. The linking sequence that will link CRS5 and GST comes from part BBa_J331010.
This main purpose of this composite is to purify water from lead, so that it is cleaner for its use. This gene can be inserted into E.coli so that the bacteria can be placed in a tank filled with contaminated water and purify the water from lead.
RBS.1 (strong) -- modified from R. Weiss
Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info Brian Chow
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
Double terminator consisting of BBa_B0012 and BBa_B0011
Overlap extension of synthetic oligonucleotides
It comes from a genomic sequence. http://www.yeastgenome.org/locus/S000003406/sequence
Mitochondrial protein required for gathering of cytochrome bc1 complex; interfaces with the Cbp3p-Cbp6p complex and recently integrated cytochrome b (Cobp) to advance get together of Cobp into the cytochrome bc1 complex. It was used by the 2014 Cornell Project as a part to remove lead from water.
Linking Sequence for Metallothionein (CRS5) and Glutathione S-Transferase (GST)
This is a linking sequence used to link two coding regions (CRS5 and GST) without any RBS. These two are used as part of the process of removing lead (Pb) from water.
Glutathione S-transferase internal domain
This is a GST internal domain designed to be between protein domains. GST domains are a type of affinity domain that can be used to purify and/or measure the quantity of proteins.
Rare codons in common model organisms were avoided along with restriction sites used in various assembly standards.
This coding region codes for the removal of heavy metals and the purification of substances. It can be used for the removal of lead, mercury, nickel, and others when combined with other biobricks to create a composite part. It was used by Cornell in 2014 to purify water from heavy metals.
Derived from luxICDABEG operon terminator of Vibrio fischeri <genbank>AF170104</genbank>.
Bidirectional transcriptional terminator consisting of a 22 bp stem-loop.
In the naturally-occuring sequence there is a mismatch in the stem of the stem loop. This can be corrected via an A->G mutation (at position 40 -- sequence coordinate/not MFOLD coordinate). The above sequence does not reflect this mutation (but the MFOLD image does). This terminator's location cannot be found using some inverted repeat detectors like PALINDROME because it is too short and contains a mismatch. This one was found with the help of Tom Knight. It lies between two coding regions that point towards eachother.
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Transcription terminator for the E.coli RNA polymerase.
Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
Conversion of the iGEM parts registry to SBOL2.1