BBa_B0011
1
BBa_B0011
LuxICDABEG (+/-)
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from luxICDABEG operon terminator of Vibrio fischeri <genbank>AF170104</genbank>.
Released HQ 2013
Bidirectional transcriptional terminator consisting of a 22 bp stem-loop.</p>
false
false
_1_
0
24
7
In stock
false
<P> <P>In the naturally-occuring sequence there is a mismatch in the stem of the stem loop. This can be corrected via an A->G mutation (at position 40 -- sequence coordinate/not MFOLD coordinate). The above sequence does not reflect this mutation (but the MFOLD image does). This terminator's location cannot be found using some inverted repeat detectors like PALINDROME because it is too short and contains a mismatch. This one was found with the help of Tom Knight. It lies between two coding regions that point towards eachother.<P>
true
Reshma Shetty
annotation7019
1
BBa_B0011
range7019
1
1
46
annotation1683
1
stem_loop
range1683
1
13
35
BBa_B0030
1
BBa_B0030
RBS.1 (strong) -- modified from R. Weiss
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
false
true
_44_46_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation1701
1
RBS-1\Strong
range1701
1
1
15
annotation1702
1
RBS
range1702
1
8
12
annotation7025
1
BBa_B0030
range7025
1
1
15
BBa_B0014
1
BBa_B0014
double terminator (B0012-B0011)
2003-07-15T11:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Double terminator consisting of BBa_B0012 and BBa_B0011
false
true
_1_
0
24
7
In stock
false
true
Reshma Shetty
component939311
1
BBa_B0011
component939303
1
BBa_B0012
annotation939311
1
BBa_B0011
range939311
1
50
95
annotation939303
1
BBa_B0012
range939303
1
1
41
BBa_J331014
1
BBa_J331014
Family 5 extracellular solute-binding protein gene sequence
2016-12-09T12:00:00Z
2016-12-09T12:15:13Z
none
extracellular solute-binding protein
false
false
_1979_
24251
24251
9
false
none
false
Gilles Yvan Buck
BBa_J331015
1
BBa_J331015
Destructor of mast cells
2016-12-09T12:00:00Z
2016-12-09T12:17:45Z
none
It destructs mast cells, neutralizing allergic response.
false
false
_1979_
24251
24251
9
false
none
false
Gilles Yvan Buck
component2531946
1
BBa_J331014
component2531947
1
BBa_J23119
component2531957
1
BBa_B0014
component2531949
1
BBa_B0030
annotation2531946
1
BBa_J331014
range2531946
1
1
630
annotation2531947
1
BBa_J23119
range2531947
1
639
673
annotation2531957
1
BBa_B0014
range2531957
1
705
799
annotation2531949
1
BBa_B0030
range2531949
1
682
696
BBa_J23119
1
BBa_J23119
constitutive promoter family member
2006-08-23T11:00:00Z
2015-08-31T04:08:40Z
Overlap extension of synthetic oligonucleotides
Released HQ 2013
Later
false
true
_52_
0
483
95
In stock
false
N/A
true
John Anderson
BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation1690
1
polya
range1690
1
28
41
annotation1686
1
T7 TE
range1686
1
8
27
annotation7020
1
BBa_B0012
range7020
1
1
41
annotation1687
1
stop
range1687
1
34
34
BBa_B0014_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt
BBa_B0030_sequence
1
attaaagaggagaaa
BBa_J331014_sequence
1
ccgggagaaactttgagttgatgaagtgaaacgtgttgtctttcagcttctggatttctaggatggggtattgccgctgggtgccagccgctgtttacagctcgggaatgggtcccttcgtgccacctccagcctactacgctcaaaaccagatcgtccccgctatcggaaaccgctgccaccgcttgtgtaactgatggcacgatgactcttcactatttaaatgtgacggacgttgtagatttgaaggcagtttttctcgagaggtttatgtcagcgatgcacttgagaccattactattaaaggagctcgaccccgaaacgtcacccattccttctctccagggatgctgcctgcccagctgagttactcagcattttgtgtctgtcttcactgttcaatcttatgtgcaggaaggaaatgcagatgctggtttataccaaatatagacgcgaaatgctggggtaagtcggcggatcaaggcagcgtgactggcgtaaaagaataggtgacgataatatttgagatccttcatctcccctcctccaccctgctcgtttaaccagttccacagttcactgcattgtatctctcttgagatcgcatcttcccaagccaacagggggcct
BBa_B0011_sequence
1
agagaatataaaaagccagattattaatccggcttttttattattt
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_J23119_sequence
1
ttgacagctagctcagtcctaggtataatgctagc
BBa_J331015_sequence
1
ccgggagaaactttgagttgatgaagtgaaacgtgttgtctttcagcttctggatttctaggatggggtattgccgctgggtgccagccgctgtttacagctcgggaatgggtcccttcgtgccacctccagcctactacgctcaaaaccagatcgtccccgctatcggaaaccgctgccaccgcttgtgtaactgatggcacgatgactcttcactatttaaatgtgacggacgttgtagatttgaaggcagtttttctcgagaggtttatgtcagcgatgcacttgagaccattactattaaaggagctcgaccccgaaacgtcacccattccttctctccagggatgctgcctgcccagctgagttactcagcattttgtgtctgtcttcactgttcaatcttatgtgcaggaaggaaatgcagatgctggtttataccaaatatagacgcgaaatgctggggtaagtcggcggatcaaggcagcgtgactggcgtaaaagaataggtgacgataatatttgagatccttcatctcccctcctccaccctgctcgtttaaccagttccacagttcactgcattgtatctctcttgagatcgcatcttcccaagccaacagggggccttactagagttgacagctagctcagtcctaggtataatgctagctactagagattaaagaggagaaatactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z