LOCUS BBa_J331021 6455 bp DNA linear 16-Jun-2024 DEFINITION Tuberculosis Detection ACCESSION BBa_J331021 VERSION BBa_J331021.1 FEATURES Location/Qualifiers CDS 211..1113 /label=NahR promoter 1192..1262 /label=pSal CDS 1275..1997 /label=sfGFP stem_loop 2004..2132 /label=B0015 misc_feature 1..2132 /label=BBa_K1031610 CDS 2141..3043 /label=NahR promoter 3044..3133 /label=Pr promoter promoter 3122..3190 /label=pSal promoter 2141..3192 /label=BBa_K228004 misc_feature 3201..3219 /label=TetR 1 misc_feature 3201..3254 /label=BBa_R0040 promoter 3220..3225 /label=-35 misc_feature 3226..3244 /label=TetR 2 promoter 3243..3248 /label=-10 promoter 3201..3254 /label=BBa_R0040 RBS 3263..3275 /label=RBS-3\Weak RBS 3269..3272 /label=RBS misc_feature 3263..3275 /label=BBa_B0032 RBS 3263..3275 /label=BBa_B0032 CDS 3282..4355 /label=BMST1 misc_feature 3282..3284 /label=start codon misc_feature 4353..4355 /label=stop codon CDS 3282..4355 /label=BBa_J45004 stem_loop 4375..4418 /label=stem_loop misc_feature 4364..4443 /label=BBa_B0010 terminator 4364..4443 /label=BBa_B0010 stem_loop 4459..4478 /label=T7 TE misc_feature 4485..4485 /label=stop polyA_site 4479..4492 /label=polya misc_feature 4452..4492 /label=BBa_B0012 terminator 4452..4492 /label=BBa_B0012 misc_feature 4503..4519 /label=lac O1 promoter 4520..4525 /label=-35 misc_feature 4526..4542 /label=lac O1 promoter 4543..4548 /label=-10 misc_feature 4501..4554 /label=BBa_R0011 promoter 4501..4554 /label=BBa_R0011 RBS 4564..4576 /label=RBS-3\Weak RBS 4570..4573 /label=RBS misc_feature 4564..4576 /label=BBa_B0032 RBS 4564..4576 /label=BBa_B0032 misc_feature 5022..5022 /label=g -> a to eliminate PstI site misc_feature 4885..4887 /label=pchA start codon misc_feature 4583..4585 /label=pchB start codon misc_feature 4886..4888 /label=pchB stop codon misc_feature 6313..6318 /label=pchA double TAA stop codon CDS 4583..4888 /label=PchB CDS 4885..6318 /label=PchA CDS 4583..6318 /label=BBa_J45017 stem_loop 6338..6381 /label=stem_loop misc_feature 6327..6406 /label=BBa_B0010 terminator 6327..6406 /label=BBa_B0010 stem_loop 6422..6441 /label=T7 TE misc_feature 6448..6448 /label=stop polyA_site 6442..6455 /label=polya misc_feature 6415..6455 /label=BBa_B0012 terminator 6415..6455 /label=BBa_B0012 misc_feature 3201..6455 /label=BBa_J45700 ORIGIN 1 ggccgctgcg atcccgcgaa gaaccaaaaa agctcgacag agggcgcggt cattttaggt 61 cgggcggatc ggcgccgccg gctcggctgg tgtgccgcac agcaccgcct acgtgagctg 121 ccagttgatg aacttccccc gttgccagct agggcgcaag cgggctgtat aagatcactg 181 cccatcacat tgatcggctc ggattttttc tcaatccgta aacaggtcaa acatcagttg 241 ccgcaaccaa atattggcta ggtccttgtg gtacttcgca tgccagaaca tgttgatggc 301 tatttcaggc aagacgactg ggtgcggcaa ggcgcttagg ccgaagggct ccacgcagca 361 gtcggctaaa cgtatcggca cagtggcgag cagatcggtg cgctggagga tgtggccaac 421 ggcggcgaag tgcggcactt ccagacggat gtcgcgccgg atgccgaccc gtgtcatgta 481 cgtgtccacc tcgccgtggc cggtgccagc ggcgatgaca cgcacgtggc cgtaggaaca 541 gaagcgctcc agagtcaggg gttcgcgggt gactggatgg tccttgcgac ataggcacac 601 gtagtgattc tggagcagcc ggcgctgaaa gaagccagtt tgcagattgg gaagcaggcc 661 cacggccaag tccacggttc cgttctgcaa ggcctgcatc aggctcatcg aactgtcgcg 721 caccgtactg atcacgcaat tgggggcctg gtgagccagc acatccatca gccgcggcat 781 gaagtagatc tcgccaatgt cggtcatggc cagggtgaag gtacgctcgc tggtcagcgg 841 atcgaagctt tcatggtgct gtagggcgtt gcgcagtgcg tgcatggccg aagtgacggg 901 ctcggccaga tgcgcggcat agggtgtggg ttccattccc tgatgtgtgc gcacgaagag 961 tgggtcctgt agcgaggtgc gcaggcgttt cagcgcattg ctcacggcag gctgggtcag 1021 gcccaggttc tccgcagtga tagagacgcg tctgtcgacc agcaactggt tgaacaccac 1081 cagcaggttt aaatccaggt cacgcagttc catggggcct cgcttgggtt attgctggtg 1141 cccggccggg cgcaatattc atgttgatga tttattatat atcgagtggt gtatttatca 1201 atattgtttg ctccgttatc gttattaaca agtcatcaat aaagccatca cgagtaccat 1261 agaggatcac tagaatgcgt aaaggcgaag agctgttcac tggtgtcgtc cctattctgg 1321 tggaactgga tggtgatgtc aacggtcata agttttccgt gcgtggcgag ggtgaaggtg 1381 acgcaactaa tggtaaactg acgctgaagt tcatctgtac tactggtaaa ctgccggtac 1441 cttggccgac tctggtaacg acgctgactt atggtgttca gtgctttgct cgttatccgg 1501 accatatgaa gcagcatgac ttcttcaagt ccgccatgcc ggaaggctat gtgcaggaac 1561 gcacgatttc ctttaaggat gacggcacgt acaaaacgcg tgcggaagtg aaatttgaag 1621 gcgataccct ggtaaaccgc attgagctga aaggcattga ctttaaagaa gacggcaata 1681 tcctgggcca taagctggaa tacaatttta acagccacaa tgtttacatc accgccgata 1741 aacaaaaaaa tggcattaaa gcgaatttta aaattcgcca caacgtggag gatggcagcg 1801 tgcagctggc tgatcactac cagcaaaaca ctccaatcgg tgatggtcct gttctgctgc 1861 cagacaatca ctatctgagc acgcaaagcg ttctgtctaa agatccgaac gagaaacgcg 1921 atcatatggt tctgctggag ttcgtaaccg cagcgggcat cacgcatggt atggatgaac 1981 tgtacaaatg atgataaact agaccaggca tcaaataaaa cgaaaggctc agtcgaaaga 2041 ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg 2101 ctcaccttcg ggtgggcctt tctgcgttta tatactagag tcaatccgta aacaggtcaa 2161 acatcagttg ccgcaaccaa atattggcta ggtccttgtg gtacttcgca tgccagaaca 2221 tgttgatggc tatttcaggc aagacgactg ggtgcggcaa ggcgcttagg ccgaagggct 2281 ccacgcagca gtcggctaaa cgtatcggca cagtggcgag cagatcggtg cgctggagga 2341 tgtggccaac ggcggcgaag tgcggcactt ccagacggat gtcgcgccgg atgccgaccc 2401 gtgtcatgta cgtgtccacc tcgccgtggc cggtgccagc ggcgatgaca cgcacgtggc 2461 cgtaggaaca gaagcgctcc agagtcaggg gttcgcgggt gactggatgg tccttgcgac 2521 ataggcacac gtagtgattc tggagcagcc ggcgctgaaa gaagccagtt tgcagattgg 2581 gaagcaggcc cacggccaag tccacggttc cgttctgcaa ggcctgcatc aggctcatcg 2641 aactgtcgcg caccgtactg atcacgcaat tgggggcctg gtgagccagc acatccatca 2701 gccgcggcat gaagtagatc tcgccaatgt cggtcatggc cagggtgaag gtacgctcgc 2761 tggtcagcgg atcgaagctt tcatggtgct gtagggcgtt gcgcagtgcg tgcatggccg 2821 aagtgacggg ctcggccaga tgcgcggcat agggtgtggg ttccattccc tgatgtgtgc 2881 gcacgaagag tgggtcctgt agcgaggtgc gcaggcgttt cagcgcattg ctcacggcag 2941 gctgggtcag gcccaggttc tccgcagtga tagagacgcg tctgtcgacc agcaactggt 3001 tgaacaccac cagcaggttt aaatccaggt cacgcagttc catggggcct cgcttgggtt 3061 attgctggtg cccggccggg cgcaatattc atgttgatga tttattatat atcgagtggt 3121 gtatttatca atattgtttg ctccgttatc gttattaaca agtcatcaat aaagccatca 3181 cgagtaccat agtactagag tccctatcag tgatagagat tgacatccct atcagtgata 3241 gagatactga gcactactag agtcacacag gaaagtacta gatggaagtt gttgaagttc 3301 ttcacatgaa tggaggaaat ggagacagta gctatgcaaa caattctttg gttcagcaaa 3361 aggtgattct catgacaaag ccaataactg agcaagccat gattgatctc tacagcagcc 3421 tctttccaga aaccttatgc attgcagatt tgggttgttc tttgggagct aacactttct 3481 tggtggtctc acagcttgtt aaaatagtag aaaaagaacg aaaaaagcat ggttttaagt 3541 ctccagagtt ttattttcac ttcaatgatc ttcctggcaa tgattttaat acactttttc 3601 agtcactggg ggcatttcaa gaagatttga gaaagcatat aggggaaagc tttggtccat 3661 gttttttcag tggagtgcct ggttcatttt atactagact tttcccttcc aaaagtttac 3721 attttgttta ctcctcctac agtctcatgt ggctatctca ggtgcctaat gggattgaaa 3781 ataacaaggg aaacatttac atggcaagaa caagccctct aagtgttatt aaagcatact 3841 acaagcaata tgaaatagat ttttcaaatt ttctcaagta ccgttcagag gaattgatga 3901 aaggtggaaa gatggtgtta acactcctag gtagagaaag tgaggatcct actagcaaag 3961 aatgctgtta catttgggag cttctagcca tggccctcaa taagttggtt gaagagggat 4021 tgataaaaga agagaaagta gatgcattca atattcctca atacacacca tcaccagcag 4081 aagtaaagta catagttgag aaggaaggat cattcaccat taatcgcttg gaaacatcaa 4141 gagttcattg gaatgcttct aataatgaga agaatggtgg ttacaatgtg tcaaggtgca 4201 tgagagctgt ggctgagcct ttgcttgtca gccactttga caaggaattg atggatttag 4261 tgttccacaa gtacgaagag attgtttctg attgcatgtc caaagagaat actgagttta 4321 taaatgtcat catctccttg accaaaataa attaatacta gagccaggca tcaaataaaa 4381 cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct 4441 ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta tatactagag 4501 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta 4561 gagtcacaca ggaaagtact agatgaaaac tcccgaagac tgcaccggcc tggcggacat 4621 ccgcgaggcc atcgaccgga tcgacctgga tatcgtccag gccctcggcc gccgcatgga 4681 ctacgtcaag gcggcgtcgc gcttcaaggc cagcgaggcg gcgattccgg cgcccgagcg 4741 ggtcgccgcg atgctccccg agcgcgcccg ctgggccgag gaaaacggac tcgacgcgcc 4801 cttcgtcgag ggactgttcg cgcagatcat ccactggtac atcgccgagc agatcaagta 4861 ctggcgccag acacggggtg ccgcatgagc cggctggcgc ccctgagcca gtgcctgcac 4921 gccttgcgcg gcaccttcga gcgcgccatc ggccaggcgc aggcgctcga tcgtccggtg 4981 ctggtggcgg catcgttcga gatcgaccca ttggacccgc tacaggtatt cggtgcctgg 5041 gacgaccggc aaacgccctg cctgtactgg gaacagcccg agctggcgtt cttcgcctgg 5101 ggctgcgccc tggagctgca aggccacggc gaacagcgct tcgcccggat cgaggaaaac 5161 tggcaattgc tctgcgccga cgccgtggtc gagggcccgc tggcgccgcg cctgtgcggc 5221 ggattccgct tcgatccgcg cggcccgcgc gaggaacact ggcaagcctt cgccgatgcc 5281 agcctgatgc tcgccggcat caccgtgctg cgcgagggcg aacgctaccg ggtactctgc 5341 caacacctgg ccaagcccgg cgaagatgcc ctggccctgg ccgcctacca ctgctcggcg 5401 ctactgcgcc tgaggcagcc ggccagacgc cggccctcgg ggccgaccgc tggcgcgcag 5461 ggcgacgctt cggcgcagga gcgcaggcaa tgggaagcca aggtgagcga cgcggtaagc 5521 agtgtccgcc agggacgctt cggcaaggtc gtgctggccc gcacccaggc ccggcctctc 5581 ggcgacatcg agccgtggca ggtcatcgaa cacctgcgtc tgcaacatgc cgacgcccag 5641 ctgttcgcct gtcgccgcgg caacgcctgc ttcctcggcg cctccccgga acgcctggtc 5701 cgcattcgcg ccggcgaggc actcacccat gccctggccg ggaccatcgc ccgcggcggc 5761 gatgcccagg aagatgcgcg gctcggacag gccctgctgg acagcgccaa ggacaggcac 5821 gaacaccagt tggtggtgga ggcgatccgt acggccctgg aacccttcag cgaggtgctg 5881 gaaatccccg atgcgcccgg cctgaaacga ctggcgcgag tccagcacct gaacacgccg 5941 atccgcgccc gcctcgctga cgcaggcggc atcctgcggc tgctacaagc gctgcatccg 6001 acccccgcgg tgggcggcta cccacgcagc gcggcgctgg actacatccg ccagcacgaa 6061 gggatggacc gcggctggta cgccgcgccg ctgggctggc tcgacggcga aggcaacggc 6121 gatttcctgg tggcgctgcg ctcggccctg ctcacgccgg gccggggcta cctgttcgcc 6181 ggctgcggtc tggtaggcga ttcggaaccg gcccacgagt atcgcgaaac ctgccttaag 6241 ctcagtgcca tgcgggaagc tctatccgcc ataggcggcc tggacgaagt gcccttgcag 6301 cgcggcgtcg cctaataata ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa 6361 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac 6421 tggctcacct tcgggtgggc ctttctgcgt ttata //