BBa_J331025 1 BBa_J331025 Anthranilate 1,2-dioxygenase - Small Subunit 2016-12-13T12:00:00Z 2016-12-13T09:03:02Z UT26S DNA, chromosome 1 In enzymology, an anthranilate 1,2-dioxygenase (deaminating, decarboxylating) (EC 1.14.12.1) is an enzyme that catalyzes the chemical reaction anthranilate + NAD(P)H + 2 H+ + O2 {\displaystyle \rightleftharpoons } \rightleftharpoons catechol + CO2 + NAD(P)+ + NH3 This enzyme belongs to the family of oxidoreductases, specifically those acting on paired donors, with O2 as oxidant and incorporation or reduction of oxygen. The oxygen incorporated need not be derived from O2 with NADH or NADPH as one donor, and incorporation of two atoms o oxygen into the other donor. false false _1979_2626_ 24251 24251 9 false N/A false Gilles Yvan Buck BBa_J331024 1 BBa_J331024 anthranilate 1,2-dioxygenase - Large Subunit 2016-12-13T12:00:00Z 2016-12-13T08:59:46Z Pseudomonas protegens Pf-5 an enzyme that catalyzes the chemical reaction anthranilate + NAD(P)H + 2 H+ + O2 {\displaystyle \rightleftharpoons } \rightleftharpoons catechol + CO2 + NAD(P)+ + NH3 This enzyme belongs to the family of oxidoreductases, specifically those acting on paired donors, with O2 as oxidant and incorporation or reduction of oxygen. The oxygen incorporated need not be derived from O2 with NADH or NADPH as one donor, and incorporation of two atoms o oxygen into the other donor. false false _1979_2626_ 24251 24251 9 false N/A false Gilles Yvan Buck BBa_R0040 1 p(tetR) TetR repressible promoter 2003-01-31T12:00:00Z 2015-05-08T01:14:14Z Lutz, R., Bujard, H., <em>Nucleic Acids Research</em> (1997) 25, 1203-1210. Released HQ 2013 Sequence for pTet inverting regulator driven by the TetR protein.</P> false true _1_ 0 24 7 In stock false <P> <P>BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.<P> true June Rhee, Connie Tao, Ty Thomson, Louis Waldman annotation1986787 1 -10 range1986787 1 43 48 annotation1986786 1 TetR 2 range1986786 1 26 44 annotation1986784 1 BBa_R0040 range1986784 1 1 54 annotation1986783 1 TetR 1 range1986783 1 1 19 annotation1986785 1 -35 range1986785 1 20 25 BBa_K902034 1 BBa_K902034 CarAd Coding Sequence 2012-09-30T11:00:00Z 2015-05-08T01:13:43Z Pseudomonas putida plasmid pCAR1.2 The CarAd gene in Pseudomonas putida codes for the ferredoxin reductase component of carbazole-1,9-dioxygenase. Together with CarAa and CarAc it forms the carbazole-1,9-dioxygenase (CARDO) enzyme which converts carbazole into 2'-aminobiphenyl-2,3-diol by cleaving one C-N bond in the original carbazole molecule. false false _1167_ 0 13454 9 In stock false Site directed mutagenesis was required to remove a NotI site. false Jeff Addison annotation2205696 1 TAA Stop Codon range2205696 1 988 990 annotation2205695 1 ATG Start Codon range2205695 1 1 3 annotation2205697 1 NotI mutation (G --> A) range2205697 1 657 657 BBa_K902032 1 BBa_K902032 CarAa Coding Sequence 2012-10-01T11:00:00Z 2015-05-08T01:13:43Z tbd tbd false false _1167_ 0 13454 9 In stock false none false Jeff Addison annotation2207748 1 ATG Start Codon range2207748 1 1 3 annotation2207749 1 TGA Stop Codon range2207749 1 1153 1155 annotation2207750 1 G --> A range2207750 1 1 1 BBa_J331026 1 BBa_J331026 Enzymatic Pathway for Denitrogenation 2016-12-13T12:00:00Z 2016-12-13T09:12:05Z Nitrogen: Nitrogen is a chemical element with symbol N and atomic number 7. It was first discovered and isolated by Scottish physician Daniel Rutherford in 1772. Although Carl Wilhelm Scheele and Henry Cavendish had independently done so at about the same time, Rutherford is generally accorded the credit because his work was published first. The name nitrogen was suggested by Jean-Antoine-Claude Chaptal in 1790, when it was found that nitrogen was present in nitric acid and nitrates; this name derives from the Greek roots &#957;&#7984;&#964;&#961;&#959;&#957; "nitre" and -&#947;&#949;&#957;&#957;&#8118;&#957; "to form". Antoine Lavoisier suggested instead the name azote, from the Greek &#940;&#950;&#969;&#964;&#953;&#954;&#972;&#962; "no life", as it is an asphyxiant gas; his name is instead used in many languages, such as French, Russian, and Turkish, and appears in the English names of some nitrogen compounds such as hydrazine, azides and azo compounds. We are going to be targeting, the compound carbazole, it contains 70% of nitrogen by mass in petroleum tailing ponds, it's hard compound to attack and if our solution works it might also work in other lesser compounds. We are going to make a bacteria that is able to convert carbazole into catechol, another compound. That doesn???t contain nitrogen or any other polluting substance. The bacteria will have an enzymatic pathway that can accomplish this. true false _1979_2626_ 24251 24251 9 false N/A false Gilles Yvan Buck component2532719 1 BBa_B0034 component2532711 1 BBa_K902033 component2532703 1 BBa_B0034 component2532720 1 BBa_J331025 component2532713 1 BBa_B0034 component2532697 1 BBa_R0040 component2532717 1 BBa_K902034 component2532708 1 BBa_B0034 component2532706 1 BBa_K902032 component2532721 1 BBa_J331024 annotation2532711 1 BBa_K902033 range2532711 1 1262 1585 annotation2532720 1 BBa_J331025 range2532720 1 2628 4889 annotation2532706 1 BBa_K902032 range2532706 1 81 1235 annotation2532713 1 BBa_B0034 range2532713 1 1594 1605 annotation2532708 1 BBa_B0034 range2532708 1 1244 1255 annotation2532719 1 BBa_B0034 range2532719 1 2610 2621 annotation2532721 1 BBa_J331024 range2532721 1 4896 6284 annotation2532703 1 BBa_B0034 range2532703 1 63 74 annotation2532717 1 BBa_K902034 range2532717 1 1612 2601 annotation2532697 1 BBa_R0040 range2532697 1 1 54 BBa_K902033 1 BBa_K902033 CarAc Coding Sequence 2012-09-30T11:00:00Z 2015-05-08T01:13:43Z Pseudomonas putida plasmid pCAR1.2 The CarAc gene in Pseudomonas codes for the ferredoxin component of carbazole-1,9-dioxygenase. Together with CarAa and CarAd it forms the carbazole-1,9-dioxygenase (CARDO) enzyme which converts carbazole into 2'-aminobiphenyl-2,3-diol by cleaving one C-N bond in the original carbazole molecule. false false _1167_ 0 13454 9 In stock false None false Jeff Addison, Anya Kornilo annotation2205029 1 ATG start codon range2205029 1 1 3 annotation2205036 1 TAG stop codon range2205036 1 322 324 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_J331026_sequence 1 tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatggcgaacgttgatgaggcaattttaaaaagagtaaaaggctgggcgccctacgtggatgcgaagctaggctttcgcaatcattggtacccggtgatgttttcgaaagagatcgacgagggcgagccgaagacactaaaactgctcggtgagaacttgctcgtcaatcgtatcgatgggaagctgtattgcctcaaggaccgctgcctgcatcgcggcgtccagttgtcggtcaaagtcgagtgcaaaacgaagtcgacgatcacatgctggtaccacgcgtggacctatcgctgggaagacggcgttctgtgcgacatcttgacgaatccgacaagcgcacagatcggtcgacaaaagctgaaaacttacccagtgcaggaagccaagggctgcgtcttcatttatcttggcgatggcgaccctcctcccttggcccgcgatacgccacccaatttccttgacgatgacatggaaatcctcgggaagaaccaaatcatcaagtctaactggcgcctcgctgtggaaaacggtttcgatccgagccacatttatattcacaaagactcaattctggtcaaggacaacgatcttgccttgccactaggtttcgcgccaggaggggatcgaaagcaacaaactcgtgtggttgacgatgacgtcgtcggacgcaagggtgtttacgatcttattggcgaacatggggtcccagtgtttgagggaactatcgggggcgaagtggtccgcgaaggtgcctacggcgaaaaaattgtagcgaacgatatctccatttggctcccgggtgttctcaaggtcaatccgttccccaatccggacatgatgcagttcgagtggtacgtgccgattgacgaaaacacacactattacttccaaactcttggcaaaccatgtgccaatgacgaggaacggaagaattacgaacaagagttcgaaagcaagtggaaaccgatggcgctcgaaggattcaacaacgatgacatctgggctcgcgaagctatggtggatttctacgccgatgataaaggctgggtcaacgagattttgttcgaggtggacgaggctatcgtggcatggcgcaagctggcgagcgaacacaatcagggtattcagacccaagcgcacgtttcgggctgatactagagaaagaggagaaatactagatgaaccaaatttggttgaaagtatgtgctgcatctgacatgcaacctggcacgatacgtcgcgtcaaccgcgtaggtgctgcacctctcgcagtctatcgtgttggcgatcagttctacgccactgaagatacgtgcacgcatggtattgcttcgctttcggaagggacactcgatggtgacgtgattgaatgtccctttcacggcggcgccttcaatgtttgtaccggcatgccggcatcaagtccatgtacagtgccgctaggagtgttcgaggtagaagtcaaagagggcgaagtttatgtcgccggagaaaagaagtagtactagagaaagaggagaaatactagatgtaccaactcaaaattgaagggcaagcgccagggacctgcggctcagggaagagcctgttggtctcagcacttgctaatggtatcggatttccgtacgagtgtgcatcgggaggttgcggagtatgcaaattcgagttactcgaagggaatgtccaatcaatgtggccggatgctccaggactttcttcgcgagatcgtgagaagggcaaccgccatcttgcatgccagtgcgttgcgctctcagacctgcggatcaaagtcgcagtgcaggacaagtacgtcccaacgattccaatctcaagaatggaagcggaagttgttgaggtccgggcgctaactcatgacctgctgtccgtgcgattacgcactgatgggccagcaaatttcctccccggccagttctgcctagtagaggcagagcagttgccaggcgtggttcgcgcatattcaatggcgaatttaaagaaccccgaaggcatatgggagttctatattaagagggtacccacaggacgatttagtccttggcttttcgaaaatagaaaagaaggcgctcgtctatttttgacgggaccaatgggcacatctttcttccgtccagggaccggccgaaagagtctttgcattggcggcggtgccgggctctcgtatgcagccgctattgcacgcgcctcgatgcgcgaaacagacaagccggtaaagttgttctacggctcaagaactccgcgcgacgctgttcggtggatcgatatcgacatcgatgaggacaagcttgaggtcgtccaggcagttacggaagacacggatagcctttggcaagggcccactggttttattcatcaggttgtcgacgcagcgctgcttgaaaccctaccggaatacgaaatttatcttgccggtccaccgcctatggtcgacgctactgtccgtatgctgctcggcaagggtgttccacgcgatcaaattcattttgacgcatttttctaatactagagaaagaggagaaatactagatgaccgacaagtcgaatgtggaattttccgagcgcgaggcgctgttcttccactcgacgggacgccccggcaagatcgagatcatcgcctcgaagccgatggcgacccagcgcgacctcagcctcgcctactcgccgggcgtcgcggtgccggtgcgcgccatcgcgaaagacccggcgaccgcctatgactataccgccaagggcaatctggtcgcggtgatctccaacggcacggcgatcctggggctgggcaatctgggcgcgctggcgtccaagccggtgatggagggcaagtcggtcctcttcaagcgcttcgccgacgtcgacagcatcgacatcgaactcaagaccgaggatgtcgacaagttcatcgacgcggtcgagctgatggagccgaccttcggcggcatcaacctggaggacatcaaggccccggaatgcttcatcatcgaacagacgctgaaggagcggatgaacattccggtcttccatgacgaccagcacggcaccgcgatcatcgcggcggcgggcgtcatcaacgcggcgatgctgaccggccgcgagatgaaggacatgaaggtggtggtgaacggcgcgggcgcggcgtccatctcctgcaccgaactgatcaaggcgctgggcgtgcagcatgacaatgtgatcatgtgcgacagcaagggcgtgatctaccagggccgcaccgagggcatgaaccagtggaagtcggcccatgcggtgaagaccgacgcccgttcgctggccgaagcggtgaagggcgcggacgtgttcctgggcctctccgtcgccggtgcgatgacgcaggacatggtgaagtccatggcggacaagccgatcatcttcgccatggcgaaccccgacccggaaatcgcgccgcccgacgccaaggcggtgcggcccgacgccatcgtcgccacgggccggtcggactatcccaaccaggtcaacaatgtgctgggcttccccttcatcttccgcggcgcgctggacgtgcgggccaccgggatcaacgaggcgatgaagctggccgccgccaaggccatcgccgaactggcgcgggaacaggtgccggaggaagtcgccaaggcctatggccgctcgcacagcttcggcccggactatatcatccccgcgcccttcgacccgcgcctgatggaggtcgtgccctccgccgtcgcgcaggcggcgatggagaccggcgtcgcgcagcggcccatcgcggacatggcggcctatcgccaatccttgaaggggcggctcaacccgaccacctccgtcctcaccaccgcctatgaggtggcgaaggccaatcccaagcgcgtcgtcttcgccgaggcggaagaggaagtagtgctgcgcgccgccatccagttccgcacccttggctatggcatccccgtgctggtcgggcgcgcccatgtctatgacaagctcaaggaactgggcgtggaggatccggaaagctacgaactgcacaacagcgtcaactcgccgctggtgccggaaatggtggacacgctctacaagcggctccagcgccggggctatctgcgccgcgattgcgagcggatggtcaatcgcgaccgtaacatcttcggctcgctgctggtgaagatgggccatgccgacgcgatgctgaccggcatgacccgtcccttcggacagacgctgcgcgaagtgaagcgggtgctggacccggcggcgggccgcacccccttcggcatccatgtgctggtgacgaaggacaagaccgtgttcctggccgacaccacggtgaacgagcgtcccacggcccaggaactggccgacatcgcggaaggtacggtggcggtcgcccggcgcatgggccatgatccgcgcgtcgcattcctgtcctattccaatttcggcaacccgccgggcggcttcctggaaaatgtccgcgacgcggtgaaggtgctggaagaccgcgaggtcgacttcgaattcgaaggggagatgaccgccgatgcggcgctcaacccgacggtgatgaagaattatcccttcagccgcctgtccggcccggccaatatattggtgatgccggggcttcagtcggccaatatctctgccaagctgctgcgcgaactgggcggcgcgaccatgatcgggccgatgctggtcggcatggacaagtcggtgcagatcgccaccatggcgtccaacgcatcggaattgctgacgctggcggtgctggcttcggccggcgtggcggtctgatactagatgagtggtgcaagaaacgtcgagcagtggcaagacttcatcggcagctgcctggactttcgtcccgcggacggggtgttccgtatcgcccgggacatgttcaccgaacccgagctgttcgacctggagatggagctgatcttcgagaagaattggatctacgcctgtcacgaaagcgagctgcccaacaaccacgacttcatcacgatgcgcgccgggcgccagccgatgatcatcacccgcgacggcgagggccggctcaatgccctggtcaacgcctgccagcaccgcggcaccaccctgacccgggttggcaagggcaaccagtccaccttcacctgccccttccatgcctggtgctacaagagcgacgggcgcctggtgaaggtcaaggcgccaggggaatacgacgagggcttcgacaaggccacccgcggcctgaagaaggcccgcatcgagagctacaagggcttcgtcttcatcagcctggacgtggccgccgacaattccctggaagacttcctcggcgacgccaggatcttcttcgacatgatggtcgcccagtcccccgacggtgagctggaggtgctgccgggcaagtccgcctacacctacgacggcaactggaagctgcagaacgagaacggcctggacggttatcacgtcagcaccgtgcactacaactacgtggccacggtgcagcaccgccagcaggtcaacgccgacagcggcgccggcagcagcgccaccctggactacagcaagctcggcgccggcgacgccagcaccgacgatggctggttcgccttcgagaacggccacagcgtgctgttcagcgaaatgcccaacccggcggtgcgctccggctacgccagcatcatgccgcgcctggtggccgagcacggccaggccaaggccgagtggatgatgcaccgcctgcgcaacctgaacatctaccccagcctgttcttcctcgaccagatcagttcccagttgcggatcatccgccccatcgcctggaacaagaccgagatcatcagccagtgcatcggggtcaagggcgagagcgacgccgaccgggaaaaccgcatccgccagttcgaggatttcttcaacgtctcgggcatgggcacgccggacgacctggtggagttccgcgaggcccagcgcggcttccaggggcgcctggagcgctggagcgacatctcccgcgggcgccagcattgggtcagcgggcccaccgccaacagcgagaacctgggcatccagccggccatgaccggcaccgagatcacccatgaggggctgtacgtcaaccagcaccgcaactggcagaaattcctcctcgacggcctgcagcgccggcccctgaaactgcgtgaggtgtga BBa_B0034_sequence 1 aaagaggagaaa BBa_R0040_sequence 1 tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac BBa_K902034_sequence 1 atgtaccaactcaaaattgaagggcaagcgccagggacctgcggctcagggaagagcctgttggtctcagcacttgctaatggtatcggatttccgtacgagtgtgcatcgggaggttgcggagtatgcaaattcgagttactcgaagggaatgtccaatcaatgtggccggatgctccaggactttcttcgcgagatcgtgagaagggcaaccgccatcttgcatgccagtgcgttgcgctctcagacctgcggatcaaagtcgcagtgcaggacaagtacgtcccaacgattccaatctcaagaatggaagcggaagttgttgaggtccgggcgctaactcatgacctgctgtccgtgcgattacgcactgatgggccagcaaatttcctccccggccagttctgcctagtagaggcagagcagttgccaggcgtggttcgcgcatattcaatggcgaatttaaagaaccccgaaggcatatgggagttctatattaagagggtacccacaggacgatttagtccttggcttttcgaaaatagaaaagaaggcgctcgtctatttttgacgggaccaatgggcacatctttcttccgtccagggaccggccgaaagagtctttgcattggcggcggtgccgggctctcgtatgcagccgctattgcacgcgcctcgatgcgcgaaacagacaagccggtaaagttgttctacggctcaagaactccgcgcgacgctgttcggtggatcgatatcgacatcgatgaggacaagcttgaggtcgtccaggcagttacggaagacacggatagcctttggcaagggcccactggttttattcatcaggttgtcgacgcagcgctgcttgaaaccctaccggaatacgaaatttatcttgccggtccaccgcctatggtcgacgctactgtccgtatgctgctcggcaagggtgttccacgcgatcaaattcattttgacgcatttttctaa BBa_K902032_sequence 1 atggcgaacgttgatgaggcaattttaaaaagagtaaaaggctgggcgccctacgtggatgcgaagctaggctttcgcaatcattggtacccggtgatgttttcgaaagagatcgacgagggcgagccgaagacactaaaactgctcggtgagaacttgctcgtcaatcgtatcgatgggaagctgtattgcctcaaggaccgctgcctgcatcgcggcgtccagttgtcggtcaaagtcgagtgcaaaacgaagtcgacgatcacatgctggtaccacgcgtggacctatcgctgggaagacggcgttctgtgcgacatcttgacgaatccgacaagcgcacagatcggtcgacaaaagctgaaaacttacccagtgcaggaagccaagggctgcgtcttcatttatcttggcgatggcgaccctcctcccttggcccgcgatacgccacccaatttccttgacgatgacatggaaatcctcgggaagaaccaaatcatcaagtctaactggcgcctcgctgtggaaaacggtttcgatccgagccacatttatattcacaaagactcaattctggtcaaggacaacgatcttgccttgccactaggtttcgcgccaggaggggatcgaaagcaacaaactcgtgtggttgacgatgacgtcgtcggacgcaagggtgtttacgatcttattggcgaacatggggtcccagtgtttgagggaactatcgggggcgaagtggtccgcgaaggtgcctacggcgaaaaaattgtagcgaacgatatctccatttggctcccgggtgttctcaaggtcaatccgttccccaatccggacatgatgcagttcgagtggtacgtgccgattgacgaaaacacacactattacttccaaactcttggcaaaccatgtgccaatgacgaggaacggaagaattacgaacaagagttcgaaagcaagtggaaaccgatggcgctcgaaggattcaacaacgatgacatctgggctcgcgaagctatggtggatttctacgccgatgataaaggctgggtcaacgagattttgttcgaggtggacgaggctatcgtggcatggcgcaagctggcgagcgaacacaatcagggtattcagacccaagcgcacgtttcgggctga BBa_J331025_sequence 1 atgaccgacaagtcgaatgtggaattttccgagcgcgaggcgctgttcttccactcgacgggacgccccggcaagatcgagatcatcgcctcgaagccgatggcgacccagcgcgacctcagcctcgcctactcgccgggcgtcgcggtgccggtgcgcgccatcgcgaaagacccggcgaccgcctatgactataccgccaagggcaatctggtcgcggtgatctccaacggcacggcgatcctggggctgggcaatctgggcgcgctggcgtccaagccggtgatggagggcaagtcggtcctcttcaagcgcttcgccgacgtcgacagcatcgacatcgaactcaagaccgaggatgtcgacaagttcatcgacgcggtcgagctgatggagccgaccttcggcggcatcaacctggaggacatcaaggccccggaatgcttcatcatcgaacagacgctgaaggagcggatgaacattccggtcttccatgacgaccagcacggcaccgcgatcatcgcggcggcgggcgtcatcaacgcggcgatgctgaccggccgcgagatgaaggacatgaaggtggtggtgaacggcgcgggcgcggcgtccatctcctgcaccgaactgatcaaggcgctgggcgtgcagcatgacaatgtgatcatgtgcgacagcaagggcgtgatctaccagggccgcaccgagggcatgaaccagtggaagtcggcccatgcggtgaagaccgacgcccgttcgctggccgaagcggtgaagggcgcggacgtgttcctgggcctctccgtcgccggtgcgatgacgcaggacatggtgaagtccatggcggacaagccgatcatcttcgccatggcgaaccccgacccggaaatcgcgccgcccgacgccaaggcggtgcggcccgacgccatcgtcgccacgggccggtcggactatcccaaccaggtcaacaatgtgctgggcttccccttcatcttccgcggcgcgctggacgtgcgggccaccgggatcaacgaggcgatgaagctggccgccgccaaggccatcgccgaactggcgcgggaacaggtgccggaggaagtcgccaaggcctatggccgctcgcacagcttcggcccggactatatcatccccgcgcccttcgacccgcgcctgatggaggtcgtgccctccgccgtcgcgcaggcggcgatggagaccggcgtcgcgcagcggcccatcgcggacatggcggcctatcgccaatccttgaaggggcggctcaacccgaccacctccgtcctcaccaccgcctatgaggtggcgaaggccaatcccaagcgcgtcgtcttcgccgaggcggaagaggaagtagtgctgcgcgccgccatccagttccgcacccttggctatggcatccccgtgctggtcgggcgcgcccatgtctatgacaagctcaaggaactgggcgtggaggatccggaaagctacgaactgcacaacagcgtcaactcgccgctggtgccggaaatggtggacacgctctacaagcggctccagcgccggggctatctgcgccgcgattgcgagcggatggtcaatcgcgaccgtaacatcttcggctcgctgctggtgaagatgggccatgccgacgcgatgctgaccggcatgacccgtcccttcggacagacgctgcgcgaagtgaagcgggtgctggacccggcggcgggccgcacccccttcggcatccatgtgctggtgacgaaggacaagaccgtgttcctggccgacaccacggtgaacgagcgtcccacggcccaggaactggccgacatcgcggaaggtacggtggcggtcgcccggcgcatgggccatgatccgcgcgtcgcattcctgtcctattccaatttcggcaacccgccgggcggcttcctggaaaatgtccgcgacgcggtgaaggtgctggaagaccgcgaggtcgacttcgaattcgaaggggagatgaccgccgatgcggcgctcaacccgacggtgatgaagaattatcccttcagccgcctgtccggcccggccaatatattggtgatgccggggcttcagtcggccaatatctctgccaagctgctgcgcgaactgggcggcgcgaccatgatcgggccgatgctggtcggcatggacaagtcggtgcagatcgccaccatggcgtccaacgcatcggaattgctgacgctggcggtgctggcttcggccggcgtggcggtctga BBa_K902033_sequence 1 atgaaccaaatttggttgaaagtatgtgctgcatctgacatgcaacctggcacgatacgtcgcgtcaaccgcgtaggtgctgcacctctcgcagtctatcgtgttggcgatcagttctacgccactgaagatacgtgcacgcatggtattgcttcgctttcggaagggacactcgatggtgacgtgattgaatgtccctttcacggcggcgccttcaatgtttgtaccggcatgccggcatcaagtccatgtacagtgccgctaggagtgttcgaggtagaagtcaaagagggcgaagtttatgtcgccggagaaaagaagtag BBa_J331024_sequence 1 atgagtggtgcaagaaacgtcgagcagtggcaagacttcatcggcagctgcctggactttcgtcccgcggacggggtgttccgtatcgcccgggacatgttcaccgaacccgagctgttcgacctggagatggagctgatcttcgagaagaattggatctacgcctgtcacgaaagcgagctgcccaacaaccacgacttcatcacgatgcgcgccgggcgccagccgatgatcatcacccgcgacggcgagggccggctcaatgccctggtcaacgcctgccagcaccgcggcaccaccctgacccgggttggcaagggcaaccagtccaccttcacctgccccttccatgcctggtgctacaagagcgacgggcgcctggtgaaggtcaaggcgccaggggaatacgacgagggcttcgacaaggccacccgcggcctgaagaaggcccgcatcgagagctacaagggcttcgtcttcatcagcctggacgtggccgccgacaattccctggaagacttcctcggcgacgccaggatcttcttcgacatgatggtcgcccagtcccccgacggtgagctggaggtgctgccgggcaagtccgcctacacctacgacggcaactggaagctgcagaacgagaacggcctggacggttatcacgtcagcaccgtgcactacaactacgtggccacggtgcagcaccgccagcaggtcaacgccgacagcggcgccggcagcagcgccaccctggactacagcaagctcggcgccggcgacgccagcaccgacgatggctggttcgccttcgagaacggccacagcgtgctgttcagcgaaatgcccaacccggcggtgcgctccggctacgccagcatcatgccgcgcctggtggccgagcacggccaggccaaggccgagtggatgatgcaccgcctgcgcaacctgaacatctaccccagcctgttcttcctcgaccagatcagttcccagttgcggatcatccgccccatcgcctggaacaagaccgagatcatcagccagtgcatcggggtcaagggcgagagcgacgccgaccgggaaaaccgcatccgccagttcgaggatttcttcaacgtctcgggcatgggcacgccggacgacctggtggagttccgcgaggcccagcgcggcttccaggggcgcctggagcgctggagcgacatctcccgcgggcgccagcattgggtcagcgggcccaccgccaacagcgagaacctgggcatccagccggccatgaccggcaccgagatcacccatgaggggctgtacgtcaaccagcaccgcaactggcagaaattcctcctcgacggcctgcagcgccggcccctgaaactgcgtgaggtgtga igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z