Nitrogen: Nitrogen is a chemical element with symbol N and atomic number 7. It was first discovered and isolated by Scottish physician Daniel Rutherford in 1772. Although Carl Wilhelm Scheele and Henry Cavendish had independently done so at about the same time, Rutherford is generally accorded the credit because his work was published first. The name nitrogen was suggested by Jean-Antoine-Claude Chaptal in 1790, when it was found that nitrogen was present in nitric acid and nitrates; this name derives from the Greek roots νἰτρον "nitre" and -γεννᾶν "to form". Antoine Lavoisier suggested instead the name azote, from the Greek άζωτικός "no life", as it is an asphyxiant gas; his name is instead used in many languages, such as French, Russian, and Turkish, and appears in the English names of some nitrogen compounds such as hydrazine, azides and azo compounds.
We are going to be targeting, the compound carbazole, it contains 70% of nitrogen by mass in petroleum tailing ponds, it's hard compound to attack and if our solution works it might also work in other lesser compounds. We are going to make a bacteria that is able to convert carbazole into catechol, another compound. That doesn???t contain nitrogen or any other polluting substance. The bacteria will have an enzymatic pathway that can accomplish this.
RBS (Elowitz 1999) -- defines RBS efficiency
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
Lutz, R., Bujard, H., Nucleic Acids Research (1997) 25, 1203-1210.
Sequence for pTet inverting regulator driven by the TetR protein.
BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.
June Rhee, Connie Tao, Ty Thomson, Louis Waldman
The CarAc gene in Pseudomonas codes for the ferredoxin component of carbazole-1,9-dioxygenase. Together with CarAa and CarAd it forms the carbazole-1,9-dioxygenase (CARDO) enzyme which converts carbazole into 2'-aminobiphenyl-2,3-diol by cleaving one C-N bond in the original carbazole molecule.
The CarAd gene in Pseudomonas putida codes for the ferredoxin reductase component of carbazole-1,9-dioxygenase. Together with CarAa and CarAc it forms the carbazole-1,9-dioxygenase (CARDO) enzyme which converts carbazole into 2'-aminobiphenyl-2,3-diol by cleaving one C-N bond in the original carbazole molecule.
Site directed mutagenesis was required to remove a NotI site.
anthranilate 1,2-dioxygenase - Large Subunit
an enzyme that catalyzes the chemical reaction anthranilate + NAD(P)H + 2 H+ + O2 {\displaystyle \rightleftharpoons } \rightleftharpoons catechol + CO2 + NAD(P)+ + NH3 This enzyme belongs to the family of oxidoreductases, specifically those acting on paired donors, with O2 as oxidant and incorporation or reduction of oxygen. The oxygen incorporated need not be derived from O2 with NADH or NADPH as one donor, and incorporation of two atoms o oxygen into the other donor.
Anthranilate 1,2-dioxygenase - Small Subunit
In enzymology, an anthranilate 1,2-dioxygenase (deaminating, decarboxylating) (EC 1.14.12.1) is an enzyme that catalyzes the chemical reaction anthranilate + NAD(P)H + 2 H+ + O2 {\displaystyle \rightleftharpoons } \rightleftharpoons catechol + CO2 + NAD(P)+ + NH3 This enzyme belongs to the family of oxidoreductases, specifically those acting on paired donors, with O2 as oxidant and incorporation or reduction of oxygen. The oxygen incorporated need not be derived from O2 with NADH or NADPH as one donor, and incorporation of two atoms o oxygen into the other donor.
Conversion of the iGEM parts registry to SBOL2.1