LOCUS BBa_J331027 6331 bp DNA linear 29-Nov-2024 DEFINITION Enzymatic Pathway for Denitrogenation ACCESSION BBa_J331027 VERSION BBa_J331027.1 FEATURES Location/Qualifiers misc_feature 1..19 /label=TetR 1 misc_feature 1..54 /label=BBa_R0040 promoter 20..25 /label=-35 misc_feature 26..44 /label=TetR 2 promoter 43..48 /label=-10 promoter 1..54 /label=BBa_R0040 misc_feature 67..70 /label=conserved RBS 63..74 /label=BBa_B0034 misc_feature 81..83 /label=ATG Start Codon misc_feature 1233..1235 /label=TGA Stop Codon misc_feature 81..81 /label=G --> A CDS 81..1235 /label=BBa_K902032 misc_feature 1248..1251 /label=conserved RBS 1244..1255 /label=BBa_B0034 misc_feature 1262..1264 /label=ATG start codon misc_feature 1583..1585 /label=TAG stop codon CDS 1262..1585 /label=BBa_K902033 misc_feature 1598..1601 /label=conserved RBS 1594..1605 /label=BBa_B0034 misc_feature 1612..1614 /label=ATG Start Codon misc_feature 2599..2601 /label=TAA Stop Codon misc_feature 2268..2268 /label=NotI mutation (G --> A) CDS 1612..2601 /label=BBa_K902034 misc_feature 2614..2617 /label=conserved RBS 2610..2621 /label=BBa_B0034 misc_feature 2628..4889 /label=BBa_J331025 misc_feature 4896..6284 /label=BBa_J331024 misc_feature 6293..6331 /label=B1006 stem_loop 6302..6323 /label=modified thr terminator polyA_site 6324..6331 /label=PolyA polyA_site 6293..6301 /label=PolyA terminator 6293..6331 /label=BBa_B1006 ORIGIN 1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag 61 agaaagagga gaaatactag atggcgaacg ttgatgaggc aattttaaaa agagtaaaag 121 gctgggcgcc ctacgtggat gcgaagctag gctttcgcaa tcattggtac ccggtgatgt 181 tttcgaaaga gatcgacgag ggcgagccga agacactaaa actgctcggt gagaacttgc 241 tcgtcaatcg tatcgatggg aagctgtatt gcctcaagga ccgctgcctg catcgcggcg 301 tccagttgtc ggtcaaagtc gagtgcaaaa cgaagtcgac gatcacatgc tggtaccacg 361 cgtggaccta tcgctgggaa gacggcgttc tgtgcgacat cttgacgaat ccgacaagcg 421 cacagatcgg tcgacaaaag ctgaaaactt acccagtgca ggaagccaag ggctgcgtct 481 tcatttatct tggcgatggc gaccctcctc ccttggcccg cgatacgcca cccaatttcc 541 ttgacgatga catggaaatc ctcgggaaga accaaatcat caagtctaac tggcgcctcg 601 ctgtggaaaa cggtttcgat ccgagccaca tttatattca caaagactca attctggtca 661 aggacaacga tcttgccttg ccactaggtt tcgcgccagg aggggatcga aagcaacaaa 721 ctcgtgtggt tgacgatgac gtcgtcggac gcaagggtgt ttacgatctt attggcgaac 781 atggggtccc agtgtttgag ggaactatcg ggggcgaagt ggtccgcgaa ggtgcctacg 841 gcgaaaaaat tgtagcgaac gatatctcca tttggctccc gggtgttctc aaggtcaatc 901 cgttccccaa tccggacatg atgcagttcg agtggtacgt gccgattgac gaaaacacac 961 actattactt ccaaactctt ggcaaaccat gtgccaatga cgaggaacgg aagaattacg 1021 aacaagagtt cgaaagcaag tggaaaccga tggcgctcga aggattcaac aacgatgaca 1081 tctgggctcg cgaagctatg gtggatttct acgccgatga taaaggctgg gtcaacgaga 1141 ttttgttcga ggtggacgag gctatcgtgg catggcgcaa gctggcgagc gaacacaatc 1201 agggtattca gacccaagcg cacgtttcgg gctgatacta gagaaagagg agaaatacta 1261 gatgaaccaa atttggttga aagtatgtgc tgcatctgac atgcaacctg gcacgatacg 1321 tcgcgtcaac cgcgtaggtg ctgcacctct cgcagtctat cgtgttggcg atcagttcta 1381 cgccactgaa gatacgtgca cgcatggtat tgcttcgctt tcggaaggga cactcgatgg 1441 tgacgtgatt gaatgtccct ttcacggcgg cgccttcaat gtttgtaccg gcatgccggc 1501 atcaagtcca tgtacagtgc cgctaggagt gttcgaggta gaagtcaaag agggcgaagt 1561 ttatgtcgcc ggagaaaaga agtagtacta gagaaagagg agaaatacta gatgtaccaa 1621 ctcaaaattg aagggcaagc gccagggacc tgcggctcag ggaagagcct gttggtctca 1681 gcacttgcta atggtatcgg atttccgtac gagtgtgcat cgggaggttg cggagtatgc 1741 aaattcgagt tactcgaagg gaatgtccaa tcaatgtggc cggatgctcc aggactttct 1801 tcgcgagatc gtgagaaggg caaccgccat cttgcatgcc agtgcgttgc gctctcagac 1861 ctgcggatca aagtcgcagt gcaggacaag tacgtcccaa cgattccaat ctcaagaatg 1921 gaagcggaag ttgttgaggt ccgggcgcta actcatgacc tgctgtccgt gcgattacgc 1981 actgatgggc cagcaaattt cctccccggc cagttctgcc tagtagaggc agagcagttg 2041 ccaggcgtgg ttcgcgcata ttcaatggcg aatttaaaga accccgaagg catatgggag 2101 ttctatatta agagggtacc cacaggacga tttagtcctt ggcttttcga aaatagaaaa 2161 gaaggcgctc gtctattttt gacgggacca atgggcacat ctttcttccg tccagggacc 2221 ggccgaaaga gtctttgcat tggcggcggt gccgggctct cgtatgcagc cgctattgca 2281 cgcgcctcga tgcgcgaaac agacaagccg gtaaagttgt tctacggctc aagaactccg 2341 cgcgacgctg ttcggtggat cgatatcgac atcgatgagg acaagcttga ggtcgtccag 2401 gcagttacgg aagacacgga tagcctttgg caagggccca ctggttttat tcatcaggtt 2461 gtcgacgcag cgctgcttga aaccctaccg gaatacgaaa tttatcttgc cggtccaccg 2521 cctatggtcg acgctactgt ccgtatgctg ctcggcaagg gtgttccacg cgatcaaatt 2581 cattttgacg catttttcta atactagaga aagaggagaa atactagatg accgacaagt 2641 cgaatgtgga attttccgag cgcgaggcgc tgttcttcca ctcgacggga cgccccggca 2701 agatcgagat catcgcctcg aagccgatgg cgacccagcg cgacctcagc ctcgcctact 2761 cgccgggcgt cgcggtgccg gtgcgcgcca tcgcgaaaga cccggcgacc gcctatgact 2821 ataccgccaa gggcaatctg gtcgcggtga tctccaacgg cacggcgatc ctggggctgg 2881 gcaatctggg cgcgctggcg tccaagccgg tgatggaggg caagtcggtc ctcttcaagc 2941 gcttcgccga cgtcgacagc atcgacatcg aactcaagac cgaggatgtc gacaagttca 3001 tcgacgcggt cgagctgatg gagccgacct tcggcggcat caacctggag gacatcaagg 3061 ccccggaatg cttcatcatc gaacagacgc tgaaggagcg gatgaacatt ccggtcttcc 3121 atgacgacca gcacggcacc gcgatcatcg cggcggcggg cgtcatcaac gcggcgatgc 3181 tgaccggccg cgagatgaag gacatgaagg tggtggtgaa cggcgcgggc gcggcgtcca 3241 tctcctgcac cgaactgatc aaggcgctgg gcgtgcagca tgacaatgtg atcatgtgcg 3301 acagcaaggg cgtgatctac cagggccgca ccgagggcat gaaccagtgg aagtcggccc 3361 atgcggtgaa gaccgacgcc cgttcgctgg ccgaagcggt gaagggcgcg gacgtgttcc 3421 tgggcctctc cgtcgccggt gcgatgacgc aggacatggt gaagtccatg gcggacaagc 3481 cgatcatctt cgccatggcg aaccccgacc cggaaatcgc gccgcccgac gccaaggcgg 3541 tgcggcccga cgccatcgtc gccacgggcc ggtcggacta tcccaaccag gtcaacaatg 3601 tgctgggctt ccccttcatc ttccgcggcg cgctggacgt gcgggccacc gggatcaacg 3661 aggcgatgaa gctggccgcc gccaaggcca tcgccgaact ggcgcgggaa caggtgccgg 3721 aggaagtcgc caaggcctat ggccgctcgc acagcttcgg cccggactat atcatccccg 3781 cgcccttcga cccgcgcctg atggaggtcg tgccctccgc cgtcgcgcag gcggcgatgg 3841 agaccggcgt cgcgcagcgg cccatcgcgg acatggcggc ctatcgccaa tccttgaagg 3901 ggcggctcaa cccgaccacc tccgtcctca ccaccgccta tgaggtggcg aaggccaatc 3961 ccaagcgcgt cgtcttcgcc gaggcggaag aggaagtagt gctgcgcgcc gccatccagt 4021 tccgcaccct tggctatggc atccccgtgc tggtcgggcg cgcccatgtc tatgacaagc 4081 tcaaggaact gggcgtggag gatccggaaa gctacgaact gcacaacagc gtcaactcgc 4141 cgctggtgcc ggaaatggtg gacacgctct acaagcggct ccagcgccgg ggctatctgc 4201 gccgcgattg cgagcggatg gtcaatcgcg accgtaacat cttcggctcg ctgctggtga 4261 agatgggcca tgccgacgcg atgctgaccg gcatgacccg tcccttcgga cagacgctgc 4321 gcgaagtgaa gcgggtgctg gacccggcgg cgggccgcac ccccttcggc atccatgtgc 4381 tggtgacgaa ggacaagacc gtgttcctgg ccgacaccac ggtgaacgag cgtcccacgg 4441 cccaggaact ggccgacatc gcggaaggta cggtggcggt cgcccggcgc atgggccatg 4501 atccgcgcgt cgcattcctg tcctattcca atttcggcaa cccgccgggc ggcttcctgg 4561 aaaatgtccg cgacgcggtg aaggtgctgg aagaccgcga ggtcgacttc gaattcgaag 4621 gggagatgac cgccgatgcg gcgctcaacc cgacggtgat gaagaattat cccttcagcc 4681 gcctgtccgg cccggccaat atattggtga tgccggggct tcagtcggcc aatatctctg 4741 ccaagctgct gcgcgaactg ggcggcgcga ccatgatcgg gccgatgctg gtcggcatgg 4801 acaagtcggt gcagatcgcc accatggcgt ccaacgcatc ggaattgctg acgctggcgg 4861 tgctggcttc ggccggcgtg gcggtctgat actagatgag tggtgcaaga aacgtcgagc 4921 agtggcaaga cttcatcggc agctgcctgg actttcgtcc cgcggacggg gtgttccgta 4981 tcgcccggga catgttcacc gaacccgagc tgttcgacct ggagatggag ctgatcttcg 5041 agaagaattg gatctacgcc tgtcacgaaa gcgagctgcc caacaaccac gacttcatca 5101 cgatgcgcgc cgggcgccag ccgatgatca tcacccgcga cggcgagggc cggctcaatg 5161 ccctggtcaa cgcctgccag caccgcggca ccaccctgac ccgggttggc aagggcaacc 5221 agtccacctt cacctgcccc ttccatgcct ggtgctacaa gagcgacggg cgcctggtga 5281 aggtcaaggc gccaggggaa tacgacgagg gcttcgacaa ggccacccgc ggcctgaaga 5341 aggcccgcat cgagagctac aagggcttcg tcttcatcag cctggacgtg gccgccgaca 5401 attccctgga agacttcctc ggcgacgcca ggatcttctt cgacatgatg gtcgcccagt 5461 cccccgacgg tgagctggag gtgctgccgg gcaagtccgc ctacacctac gacggcaact 5521 ggaagctgca gaacgagaac ggcctggacg gttatcacgt cagcaccgtg cactacaact 5581 acgtggccac ggtgcagcac cgccagcagg tcaacgccga cagcggcgcc ggcagcagcg 5641 ccaccctgga ctacagcaag ctcggcgccg gcgacgccag caccgacgat ggctggttcg 5701 ccttcgagaa cggccacagc gtgctgttca gcgaaatgcc caacccggcg gtgcgctccg 5761 gctacgccag catcatgccg cgcctggtgg ccgagcacgg ccaggccaag gccgagtgga 5821 tgatgcaccg cctgcgcaac ctgaacatct accccagcct gttcttcctc gaccagatca 5881 gttcccagtt gcggatcatc cgccccatcg cctggaacaa gaccgagatc atcagccagt 5941 gcatcggggt caagggcgag agcgacgccg accgggaaaa ccgcatccgc cagttcgagg 6001 atttcttcaa cgtctcgggc atgggcacgc cggacgacct ggtggagttc cgcgaggccc 6061 agcgcggctt ccaggggcgc ctggagcgct ggagcgacat ctcccgcggg cgccagcatt 6121 gggtcagcgg gcccaccgcc aacagcgaga acctgggcat ccagccggcc atgaccggca 6181 ccgagatcac ccatgagggg ctgtacgtca accagcaccg caactggcag aaattcctcc 6241 tcgacggcct gcagcgccgg cccctgaaac tgcgtgaggt gtgatactag agaaaaaaaa 6301 accccgcccc tgacagggcg gggttttttt t //