BBa_J331027
type: ComponentDefinition
persistent identity: BBa J331027
display id: BBa_J331027
version: 1
was generated by: iGEM to SBOL conversion
description: Enzymatic Pathway for Denitrogenation
created: 2016-12-13T12:00:00Z
modified: 2016-12-13T09:20:59Z
mutable provenance: Nitrogen: Nitrogen is a chemical element with symbol N and atomic number 7. It was first discovered and isolated by Scottish physician Daniel Rutherford in 1772. Although Carl Wilhelm Scheele and Henry Cavendish had independently done so at about the same time, Rutherford is generally accorded the credit because his work was published first. The name nitrogen was suggested by Jean-Antoine-Claude Chaptal in 1790, when it was found that nitrogen was present in nitric acid and nitrates; this name derives from the Greek roots νἰτρον "nitre" and -γεννᾶν "to form". Antoine Lavoisier suggested instead the name azote, from the Greek άζωτικός "no life", as it is an asphyxiant gas; his name is instead used in many languages, such as French, Russian, and Turkish, and appears in the English names of some nitrogen compounds such as hydrazine, azides and azo compounds.
owned by: james
owned by: myers
mutable description: We are going to be targeting, the compound carbazole, it contains 70% of nitrogen by mass in petroleum tailing ponds, it's hard compound to attack and if our solution works it might also work in other lesser compounds. We are going to make a bacteria that is able to convert carbazole into catechol, another compound. That doesn???t contain nitrogen or any other polluting substance. The bacteria will have an enzymatic pathway that can accomplish this.
discontinued: false
dominant: false
group u list: _1979_2626_
m user id: 24251
owner id: 24251
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: N/A
star: false
creator: Gilles Yvan Buck
type: DnaRegion
role: SO:0000110
role: partType/Temporary
component: BBa_B0034
component: BBa_K902033
component: BBa_K902034
component: BBa_R0040
component: BBa_K902032
component: BBa_J331024
component: BBa_J331025
component: BBa_B1006
sequence annotation: BBa_R0040
sequence annotation: BBa_B0034
sequence annotation: BBa_K902032
sequence annotation: BBa_K902033
sequence annotation: BBa_K902034
sequence annotation: BBa_J331025
sequence annotation: BBa_J331024
sequence annotation: BBa_B1006
sequence: https://synbiohub.org/public/igem/BBa_J331027_sequence/1

BBa_B0034
type: Component
persistent identity: component2532738
display id: component2532738
version: 1
top level: BBa_J331027
definition: BBa_B0034
access: public

BBa_K902033
type: Component
persistent identity: component2532736
display id: component2532736
version: 1
top level: BBa_J331027
definition: BBa_K902033
access: public

BBa_B0034
type: Component
persistent identity: component2532744
display id: component2532744
version: 1
top level: BBa_J331027
definition: BBa_B0034
access: public

BBa_K902034
type: Component
persistent identity: component2532742
display id: component2532742
version: 1
top level: BBa_J331027
definition: BBa_K902034
access: public

BBa_B0034
type: Component
persistent identity: component2532728
display id: component2532728
version: 1
top level: BBa_J331027
definition: BBa_B0034
access: public

BBa_R0040
type: Component
persistent identity: component2532722
display id: component2532722
version: 1
top level: BBa_J331027
definition: p(tetR)
access: public

BBa_B0034
type: Component
persistent identity: component2532733
display id: component2532733
version: 1
top level: BBa_J331027
definition: BBa_B0034
access: public

BBa_K902032
type: Component
persistent identity: component2532731
display id: component2532731
version: 1
top level: BBa_J331027
definition: BBa_K902032
access: public

BBa_J331024
type: Component
persistent identity: component2532746
display id: component2532746
version: 1
top level: BBa_J331027
definition: BBa_J331024
access: public

BBa_J331025
type: Component
persistent identity: component2532745
display id: component2532745
version: 1
top level: BBa_J331027
definition: BBa_J331025
access: public

BBa_B1006
type: Component
persistent identity: component2532751
display id: component2532751
version: 1
top level: BBa_J331027
definition: BBa_B1006
access: public

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2532722
display id: annotation2532722
version: 1
top level: BBa_J331027
location: https://synbiohub.org/public/igem/BBa_J331027/annotation2532722/range2532722/1
component: BBa_R0040

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type: Range
persistent identity: range2532722
display id: range2532722
version: 1
top level: BBa_J331027
direction: direction/forward
start: 1
end: 54
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2532728
display id: annotation2532728
version: 1
top level: BBa_J331027
location: https://synbiohub.org/public/igem/BBa_J331027/annotation2532728/range2532728/1
component: BBa_B0034

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persistent identity: range2532728
display id: range2532728
version: 1
top level: BBa_J331027
direction: direction/reverse
start: 63
end: 74
orientation: inline

BBa_K902032
type: SequenceAnnotation
persistent identity: annotation2532731
display id: annotation2532731
version: 1
top level: BBa_J331027
location: https://synbiohub.org/public/igem/BBa_J331027/annotation2532731/range2532731/1
component: BBa_K902032

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persistent identity: range2532731
display id: range2532731
version: 1
top level: BBa_J331027
direction: direction/reverse
start: 81
end: 1235
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2532733
display id: annotation2532733
version: 1
top level: BBa_J331027
location: https://synbiohub.org/public/igem/BBa_J331027/annotation2532733/range2532733/1
component: BBa_B0034

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type: Range
persistent identity: range2532733
display id: range2532733
version: 1
top level: BBa_J331027
direction: direction/reverse
start: 1244
end: 1255
orientation: inline

BBa_K902033
type: SequenceAnnotation
persistent identity: annotation2532736
display id: annotation2532736
version: 1
top level: BBa_J331027
location: https://synbiohub.org/public/igem/BBa_J331027/annotation2532736/range2532736/1
component: BBa_K902033

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type: Range
persistent identity: range2532736
display id: range2532736
version: 1
top level: BBa_J331027
direction: direction/reverse
start: 1262
end: 1585
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2532738
display id: annotation2532738
version: 1
top level: BBa_J331027
location: https://synbiohub.org/public/igem/BBa_J331027/annotation2532738/range2532738/1
component: BBa_B0034

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persistent identity: range2532738
display id: range2532738
version: 1
top level: BBa_J331027
direction: direction/reverse
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end: 1605
orientation: inline

BBa_K902034
type: SequenceAnnotation
persistent identity: annotation2532742
display id: annotation2532742
version: 1
top level: BBa_J331027
location: https://synbiohub.org/public/igem/BBa_J331027/annotation2532742/range2532742/1
component: BBa_K902034

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type: Range
persistent identity: range2532742
display id: range2532742
version: 1
top level: BBa_J331027
direction: direction/reverse
start: 1612
end: 2601
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2532744
display id: annotation2532744
version: 1
top level: BBa_J331027
location: https://synbiohub.org/public/igem/BBa_J331027/annotation2532744/range2532744/1
component: BBa_B0034

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type: Range
persistent identity: range2532744
display id: range2532744
version: 1
top level: BBa_J331027
direction: direction/reverse
start: 2610
end: 2621
orientation: inline

BBa_J331025
type: SequenceAnnotation
persistent identity: annotation2532745
display id: annotation2532745
version: 1
top level: BBa_J331027
location: https://synbiohub.org/public/igem/BBa_J331027/annotation2532745/range2532745/1
component: BBa_J331025

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type: Range
persistent identity: range2532745
display id: range2532745
version: 1
top level: BBa_J331027
direction: direction/reverse
start: 2628
end: 4889
orientation: inline

BBa_J331024
type: SequenceAnnotation
persistent identity: annotation2532746
display id: annotation2532746
version: 1
top level: BBa_J331027
location: https://synbiohub.org/public/igem/BBa_J331027/annotation2532746/range2532746/1
component: BBa_J331024

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type: Range
persistent identity: range2532746
display id: range2532746
version: 1
top level: BBa_J331027
direction: direction/reverse
start: 4896
end: 6284
orientation: inline

BBa_B1006
type: SequenceAnnotation
persistent identity: annotation2532751
display id: annotation2532751
version: 1
top level: BBa_J331027
location: https://synbiohub.org/public/igem/BBa_J331027/annotation2532751/range2532751/1
component: BBa_B1006

https://synbiohub.org/public/igem/BBa_J331027/annotation2532751/range2532751/1
type: Range
persistent identity: range2532751
display id: range2532751
version: 1
top level: BBa_J331027
direction: direction/reverse
start: 6293
end: 6331
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

p(tetR)
type: ComponentDefinition
persistent identity: BBa R0040
display id: BBa_R0040
version: 1
was generated by: iGEM to SBOL conversion
description: TetR repressible promoter
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: Lutz, R., Bujard, H., Nucleic Acids Research (1997) 25, 1203-1210.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Sequence for pTet inverting regulator driven by the TetR protein.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0040
sequence annotation: TetR 1
sequence annotation: -35
sequence annotation: TetR 2
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0040_sequence/1

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1986784
display id: annotation1986784
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
type: Range
persistent identity: range1986784
display id: range1986784
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 54
orientation: inline

TetR 1
type: SequenceAnnotation
persistent identity: annotation1986783
display id: annotation1986783
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
type: Range
persistent identity: range1986783
display id: range1986783
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 19
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1986785
display id: annotation1986785
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
type: Range
persistent identity: range1986785
display id: range1986785
version: 1
top level: p(tetR)
direction: direction/forward
start: 20
end: 25
orientation: inline

TetR 2
type: SequenceAnnotation
persistent identity: annotation1986786
display id: annotation1986786
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
type: Range
persistent identity: range1986786
display id: range1986786
version: 1
top level: p(tetR)
direction: direction/forward
start: 26
end: 44
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1986787
display id: annotation1986787
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
type: Range
persistent identity: range1986787
display id: range1986787
version: 1
top level: p(tetR)
direction: direction/forward
start: 43
end: 48
orientation: inline

BBa_B1006
type: ComponentDefinition
persistent identity: BBa B1006
display id: BBa_B1006
version: 1
was generated by: iGEM to SBOL conversion
description: Terminator (artificial, large, %T~>90)
created: 2006-08-30T11:00:00Z
modified: 2015-08-31T04:07:21Z
mutable provenance: modified E. coli thr terminator, replaced all A-T pairs in stem with C-G pairs
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Artificial terminator, estimated %T~>90% *8bp stem, 6nt loop *Bidirectional, estimated reverse %T~>90%
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_
m user id: 0
owner id: 745
owning group id: 41
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Bidirectional, with the reverse estimated to be less effective than the forward. Has a polyA tail of 9 residues.
star: true
creator: Haiyao Huang
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: B1006
sequence annotation: PolyA
sequence annotation: modified thr terminator
sequence: https://synbiohub.org/public/igem/BBa_B1006_sequence/1

B1006
type: SequenceAnnotation
persistent identity: annotation1898428
display id: annotation1898428
version: 1
top level: BBa_B1006
location: https://synbiohub.org/public/igem/BBa_B1006/annotation1898428/range1898428/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B1006/annotation1898428/range1898428/1
type: Range
persistent identity: range1898428
display id: range1898428
version: 1
top level: BBa_B1006
direction: direction/forward
start: 1
end: 39
orientation: inline

PolyA
type: SequenceAnnotation
persistent identity: annotation1898431
display id: annotation1898431
version: 1
top level: BBa_B1006
location: https://synbiohub.org/public/igem/BBa_B1006/annotation1898431/range1898431/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B1006/annotation1898431/range1898431/1
type: Range
persistent identity: range1898431
display id: range1898431
version: 1
top level: BBa_B1006
direction: direction/reverse
start: 1
end: 9
orientation: inline

modified thr terminator
type: SequenceAnnotation
persistent identity: annotation1898429
display id: annotation1898429
version: 1
top level: BBa_B1006
location: https://synbiohub.org/public/igem/BBa_B1006/annotation1898429/range1898429/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B1006/annotation1898429/range1898429/1
type: Range
persistent identity: range1898429
display id: range1898429
version: 1
top level: BBa_B1006
direction: direction/forward
start: 10
end: 31
orientation: inline

PolyA
type: SequenceAnnotation
persistent identity: annotation1898430
display id: annotation1898430
version: 1
top level: BBa_B1006
location: https://synbiohub.org/public/igem/BBa_B1006/annotation1898430/range1898430/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B1006/annotation1898430/range1898430/1
type: Range
persistent identity: range1898430
display id: range1898430
version: 1
top level: BBa_B1006
direction: direction/forward
start: 32
end: 39
orientation: inline

BBa_K902033
type: ComponentDefinition
persistent identity: BBa K902033
display id: BBa_K902033
version: 1
was generated by: iGEM to SBOL conversion
description: CarAc Coding Sequence
created: 2012-09-30T11:00:00Z
modified: 2015-05-08T01:13:43Z
mutable provenance: Pseudomonas putida plasmid pCAR1.2
owned by: james
owned by: myers
mutable description: The CarAc gene in Pseudomonas codes for the ferredoxin component of carbazole-1,9-dioxygenase. Together with CarAa and CarAd it forms the carbazole-1,9-dioxygenase (CARDO) enzyme which converts carbazole into 2'-aminobiphenyl-2,3-diol by cleaving one C-N bond in the original carbazole molecule.
discontinued: false
dominant: false
experience: experience/None
group u list: _1167_
m user id: 0
owner id: 13454
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: None
star: false
creator: Jeff Addison, Anya Kornilo
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: ATG start codon
sequence annotation: TAG stop codon
sequence: https://synbiohub.org/public/igem/BBa_K902033_sequence/1

ATG start codon
type: SequenceAnnotation
persistent identity: annotation2205029
display id: annotation2205029
version: 1
top level: BBa_K902033
location: https://synbiohub.org/public/igem/BBa_K902033/annotation2205029/range2205029/1
role: SO:0000318
role: feature/start

https://synbiohub.org/public/igem/BBa_K902033/annotation2205029/range2205029/1
type: Range
persistent identity: range2205029
display id: range2205029
version: 1
top level: BBa_K902033
direction: direction/forward
start: 1
end: 3
orientation: inline

TAG stop codon
type: SequenceAnnotation
persistent identity: annotation2205036
display id: annotation2205036
version: 1
top level: BBa_K902033
location: https://synbiohub.org/public/igem/BBa_K902033/annotation2205036/range2205036/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_K902033/annotation2205036/range2205036/1
type: Range
persistent identity: range2205036
display id: range2205036
version: 1
top level: BBa_K902033
direction: direction/forward
start: 322
end: 324
orientation: inline

BBa_K902034
type: ComponentDefinition
persistent identity: BBa K902034
display id: BBa_K902034
version: 1
was generated by: iGEM to SBOL conversion
description: CarAd Coding Sequence
created: 2012-09-30T11:00:00Z
modified: 2015-05-08T01:13:43Z
mutable provenance: Pseudomonas putida plasmid pCAR1.2
owned by: james
owned by: myers
mutable description: The CarAd gene in Pseudomonas putida codes for the ferredoxin reductase component of carbazole-1,9-dioxygenase. Together with CarAa and CarAc it forms the carbazole-1,9-dioxygenase (CARDO) enzyme which converts carbazole into 2'-aminobiphenyl-2,3-diol by cleaving one C-N bond in the original carbazole molecule.
discontinued: false
dominant: false
group u list: _1167_
m user id: 0
owner id: 13454
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Site directed mutagenesis was required to remove a NotI site.
star: false
creator: Jeff Addison
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: ATG Start Codon
sequence annotation: NotI mutation (G --> A)
sequence annotation: TAA Stop Codon
sequence: https://synbiohub.org/public/igem/BBa_K902034_sequence/1

ATG Start Codon
type: SequenceAnnotation
persistent identity: annotation2205695
display id: annotation2205695
version: 1
top level: BBa_K902034
location: https://synbiohub.org/public/igem/BBa_K902034/annotation2205695/range2205695/1
role: SO:0000318
role: feature/start

https://synbiohub.org/public/igem/BBa_K902034/annotation2205695/range2205695/1
type: Range
persistent identity: range2205695
display id: range2205695
version: 1
top level: BBa_K902034
direction: direction/forward
start: 1
end: 3
orientation: inline

NotI mutation (G --> A)
type: SequenceAnnotation
persistent identity: annotation2205697
display id: annotation2205697
version: 1
top level: BBa_K902034
location: https://synbiohub.org/public/igem/BBa_K902034/annotation2205697/range2205697/1
role: feature/mutation
role: SO:0001059

https://synbiohub.org/public/igem/BBa_K902034/annotation2205697/range2205697/1
type: Range
persistent identity: range2205697
display id: range2205697
version: 1
top level: BBa_K902034
direction: direction/forward
start: 657
end: 657
orientation: inline

TAA Stop Codon
type: SequenceAnnotation
persistent identity: annotation2205696
display id: annotation2205696
version: 1
top level: BBa_K902034
location: https://synbiohub.org/public/igem/BBa_K902034/annotation2205696/range2205696/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_K902034/annotation2205696/range2205696/1
type: Range
persistent identity: range2205696
display id: range2205696
version: 1
top level: BBa_K902034
direction: direction/forward
start: 988
end: 990
orientation: inline

BBa_K902032
type: ComponentDefinition
persistent identity: BBa K902032
display id: BBa_K902032
version: 1
was generated by: iGEM to SBOL conversion
description: CarAa Coding Sequence
created: 2012-10-01T11:00:00Z
modified: 2015-05-08T01:13:43Z
mutable provenance: tbd
owned by: james
owned by: myers
mutable description: tbd
discontinued: false
dominant: false
experience: experience/None
group u list: _1167_
m user id: 0
owner id: 13454
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: none
star: false
creator: Jeff Addison
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: G --> A
sequence annotation: ATG Start Codon
sequence annotation: TGA Stop Codon
sequence: https://synbiohub.org/public/igem/BBa_K902032_sequence/1

G --> A
type: SequenceAnnotation
persistent identity: annotation2207750
display id: annotation2207750
version: 1
top level: BBa_K902032
location: https://synbiohub.org/public/igem/BBa_K902032/annotation2207750/range2207750/1
role: SO:0001059
role: feature/mutation

https://synbiohub.org/public/igem/BBa_K902032/annotation2207750/range2207750/1
type: Range
persistent identity: range2207750
display id: range2207750
version: 1
top level: BBa_K902032
direction: direction/forward
start: 1
end: 1
orientation: inline

ATG Start Codon
type: SequenceAnnotation
persistent identity: annotation2207748
display id: annotation2207748
version: 1
top level: BBa_K902032
location: https://synbiohub.org/public/igem/BBa_K902032/annotation2207748/range2207748/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_K902032/annotation2207748/range2207748/1
type: Range
persistent identity: range2207748
display id: range2207748
version: 1
top level: BBa_K902032
direction: direction/forward
start: 1
end: 3
orientation: inline

TGA Stop Codon
type: SequenceAnnotation
persistent identity: annotation2207749
display id: annotation2207749
version: 1
top level: BBa_K902032
location: https://synbiohub.org/public/igem/BBa_K902032/annotation2207749/range2207749/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_K902032/annotation2207749/range2207749/1
type: Range
persistent identity: range2207749
display id: range2207749
version: 1
top level: BBa_K902032
direction: direction/forward
start: 1153
end: 1155
orientation: inline

BBa_J331024
type: ComponentDefinition
persistent identity: BBa J331024
display id: BBa_J331024
version: 1
was generated by: iGEM to SBOL conversion
description: anthranilate 1,2-dioxygenase - Large Subunit
created: 2016-12-13T12:00:00Z
modified: 2016-12-13T08:59:46Z
mutable provenance: Pseudomonas protegens Pf-5
owned by: james
owned by: myers
mutable description: an enzyme that catalyzes the chemical reaction anthranilate + NAD(P)H + 2 H+ + O2 {\displaystyle \rightleftharpoons } \rightleftharpoons catechol + CO2 + NAD(P)+ + NH3 This enzyme belongs to the family of oxidoreductases, specifically those acting on paired donors, with O2 as oxidant and incorporation or reduction of oxygen. The oxygen incorporated need not be derived from O2 with NADH or NADPH as one donor, and incorporation of two atoms o oxygen into the other donor.
discontinued: false
dominant: false
group u list: _1979_2626_
m user id: 24251
owner id: 24251
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: N/A
star: false
creator: Gilles Yvan Buck
type: DnaRegion
role: partType/Temporary
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_J331024_sequence/1

BBa_J331025
type: ComponentDefinition
persistent identity: BBa J331025
display id: BBa_J331025
version: 1
was generated by: iGEM to SBOL conversion
description: Anthranilate 1,2-dioxygenase - Small Subunit
created: 2016-12-13T12:00:00Z
modified: 2016-12-13T09:03:02Z
mutable provenance: UT26S DNA, chromosome 1
owned by: james
owned by: myers
mutable description: In enzymology, an anthranilate 1,2-dioxygenase (deaminating, decarboxylating) (EC 1.14.12.1) is an enzyme that catalyzes the chemical reaction anthranilate + NAD(P)H + 2 H+ + O2 {\displaystyle \rightleftharpoons } \rightleftharpoons catechol + CO2 + NAD(P)+ + NH3 This enzyme belongs to the family of oxidoreductases, specifically those acting on paired donors, with O2 as oxidant and incorporation or reduction of oxygen. The oxygen incorporated need not be derived from O2 with NADH or NADPH as one donor, and incorporation of two atoms o oxygen into the other donor.
discontinued: false
dominant: false
group u list: _1979_2626_
m user id: 24251
owner id: 24251
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: N/A
star: false
creator: Gilles Yvan Buck
type: DnaRegion
role: partType/Temporary
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_J331025_sequence/1

https://synbiohub.org/public/igem/BBa_J331027_sequence/1
type: Sequence
persistent identity: BBa J331027 sequence
display id: BBa_J331027_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatggcgaacgttgatgaggcaattttaaaaagagtaaaaggctgggcgccctacgtggatgcgaagctaggctttcgcaatcattggtacccggtgatgttttcgaaagagatcgacgagggcgagccgaagacactaaaactgctcggtgagaacttgctcgtcaatcgtatcgatgggaagctgtattgcctcaaggaccgctgcctgcatcgcggcgtccagttgtcggtcaaagtcgagtgcaaaacgaagtcgacgatcacatgctggtaccacgcgtggacctatcgctgggaagacggcgttctgtgcgacatcttgacgaatccgacaagcgcacagatcggtcgacaaaagctgaaaacttacccagtgcaggaagccaagggctgcgtcttcatttatcttggcgatggcgaccctcctcccttggcccgcgatacgccacccaatttccttgacgatgacatggaaatcctcgggaagaaccaaatcatcaagtctaactggcgcctcgctgtggaaaacggtttcgatccgagccacatttatattcacaaagactcaattctggtcaaggacaacgatcttgccttgccactaggtttcgcgccaggaggggatcgaaagcaacaaactcgtgtggttgacgatgacgtcgtcggacgcaagggtgtttacgatcttattggcgaacatggggtcccagtgtttgagggaactatcgggggcgaagtggtccgcgaaggtgcctacggcgaaaaaattgtagcgaacgatatctccatttggctcccgggtgttctcaaggtcaatccgttccccaatccggacatgatgcagttcgagtggtacgtgccgattgacgaaaacacacactattacttccaaactcttggcaaaccatgtgccaatgacgaggaacggaagaattacgaacaagagttcgaaagcaagtggaaaccgatggcgctcgaaggattcaacaacgatgacatctgggctcgcgaagctatggtggatttctacgccgatgataaaggctgggtcaacgagattttgttcgaggtggacgaggctatcgtggcatggcgcaagctggcgagcgaacacaatcagggtattcagacccaagcgcacgtttcgggctgatactagagaaagaggagaaatactagatgaaccaaatttggttgaaagtatgtgctgcatctgacatgcaacctggcacgatacgtcgcgtcaaccgcgtaggtgctgcacctctcgcagtctatcgtgttggcgatcagttctacgccactgaagatacgtgcacgcatggtattgcttcgctttcggaagggacactcgatggtgacgtgattgaatgtccctttcacggcggcgccttcaatgtttgtaccggcatgccggcatcaagtccatgtacagtgccgctaggagtgttcgaggtagaagtcaaagagggcgaagtttatgtcgccggagaaaagaagtagtactagagaaagaggagaaatactagatgtaccaactcaaaattgaagggcaagcgccagggacctgcggctcagggaagagcctgttggtctcagcacttgctaatggtatcggatttccgtacgagtgtgcatcgggaggttgcggagtatgcaaattcgagttactcgaagggaatgtccaatcaatgtggccggatgctccaggactttcttcgcgagatcgtgagaagggcaaccgccatcttgcatgccagtgcgttgcgctctcagacctgcggatcaaagtcgcagtgcaggacaagtacgtcccaacgattccaatctcaagaatggaagcggaagttgttgaggtccgggcgctaactcatgacctgctgtccgtgcgattacgcactgatgggccagcaaattt…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0040_sequence/1
type: Sequence
persistent identity: BBa R0040 sequence
display id: BBa_R0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B1006_sequence/1
type: Sequence
persistent identity: BBa B1006 sequence
display id: BBa_B1006_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaaaaaaaaccccgcccctgacagggcggggtttttttt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K902033_sequence/1
type: Sequence
persistent identity: BBa K902033 sequence
display id: BBa_K902033_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaaccaaatttggttgaaagtatgtgctgcatctgacatgcaacctggcacgatacgtcgcgtcaaccgcgtaggtgctgcacctctcgcagtctatcgtgttggcgatcagttctacgccactgaagatacgtgcacgcatggtattgcttcgctttcggaagggacactcgatggtgacgtgattgaatgtccctttcacggcggcgccttcaatgtttgtaccggcatgccggcatcaagtccatgtacagtgccgctaggagtgttcgaggtagaagtcaaagagggcgaagtttatgtcgccggagaaaagaagtag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K902034_sequence/1
type: Sequence
persistent identity: BBa K902034 sequence
display id: BBa_K902034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgtaccaactcaaaattgaagggcaagcgccagggacctgcggctcagggaagagcctgttggtctcagcacttgctaatggtatcggatttccgtacgagtgtgcatcgggaggttgcggagtatgcaaattcgagttactcgaagggaatgtccaatcaatgtggccggatgctccaggactttcttcgcgagatcgtgagaagggcaaccgccatcttgcatgccagtgcgttgcgctctcagacctgcggatcaaagtcgcagtgcaggacaagtacgtcccaacgattccaatctcaagaatggaagcggaagttgttgaggtccgggcgctaactcatgacctgctgtccgtgcgattacgcactgatgggccagcaaatttcctccccggccagttctgcctagtagaggcagagcagttgccaggcgtggttcgcgcatattcaatggcgaatttaaagaaccccgaaggcatatgggagttctatattaagagggtacccacaggacgatttagtccttggcttttcgaaaatagaaaagaaggcgctcgtctatttttgacgggaccaatgggcacatctttcttccgtccagggaccggccgaaagagtctttgcattggcggcggtgccgggctctcgtatgcagccgctattgcacgcgcctcgatgcgcgaaacagacaagccggtaaagttgttctacggctcaagaactccgcgcgacgctgttcggtggatcgatatcgacatcgatgaggacaagcttgaggtcgtccaggcagttacggaagacacggatagcctttggcaagggcccactggttttattcatcaggttgtcgacgcagcgctgcttgaaaccctaccggaatacgaaatttatcttgccggtccaccgcctatggtcgacgctactgtccgtatgctgctcggcaagggtgttccacgcgatcaaattcattttgacgcatttttctaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K902032_sequence/1
type: Sequence
persistent identity: BBa K902032 sequence
display id: BBa_K902032_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggcgaacgttgatgaggcaattttaaaaagagtaaaaggctgggcgccctacgtggatgcgaagctaggctttcgcaatcattggtacccggtgatgttttcgaaagagatcgacgagggcgagccgaagacactaaaactgctcggtgagaacttgctcgtcaatcgtatcgatgggaagctgtattgcctcaaggaccgctgcctgcatcgcggcgtccagttgtcggtcaaagtcgagtgcaaaacgaagtcgacgatcacatgctggtaccacgcgtggacctatcgctgggaagacggcgttctgtgcgacatcttgacgaatccgacaagcgcacagatcggtcgacaaaagctgaaaacttacccagtgcaggaagccaagggctgcgtcttcatttatcttggcgatggcgaccctcctcccttggcccgcgatacgccacccaatttccttgacgatgacatggaaatcctcgggaagaaccaaatcatcaagtctaactggcgcctcgctgtggaaaacggtttcgatccgagccacatttatattcacaaagactcaattctggtcaaggacaacgatcttgccttgccactaggtttcgcgccaggaggggatcgaaagcaacaaactcgtgtggttgacgatgacgtcgtcggacgcaagggtgtttacgatcttattggcgaacatggggtcccagtgtttgagggaactatcgggggcgaagtggtccgcgaaggtgcctacggcgaaaaaattgtagcgaacgatatctccatttggctcccgggtgttctcaaggtcaatccgttccccaatccggacatgatgcagttcgagtggtacgtgccgattgacgaaaacacacactattacttccaaactcttggcaaaccatgtgccaatgacgaggaacggaagaattacgaacaagagttcgaaagcaagtggaaaccgatggcgctcgaaggattcaacaacgatgacatctgggctcgcgaagctatggtggatttctacgccgatgataaaggctgggtcaacgagattttgttcgaggtggacgaggctatcgtggcatggcgcaagctggcgagcgaacacaatcagggtattcagacccaagcgcacgtttcgggctga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J331024_sequence/1
type: Sequence
persistent identity: BBa J331024 sequence
display id: BBa_J331024_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgagtggtgcaagaaacgtcgagcagtggcaagacttcatcggcagctgcctggactttcgtcccgcggacggggtgttccgtatcgcccgggacatgttcaccgaacccgagctgttcgacctggagatggagctgatcttcgagaagaattggatctacgcctgtcacgaaagcgagctgcccaacaaccacgacttcatcacgatgcgcgccgggcgccagccgatgatcatcacccgcgacggcgagggccggctcaatgccctggtcaacgcctgccagcaccgcggcaccaccctgacccgggttggcaagggcaaccagtccaccttcacctgccccttccatgcctggtgctacaagagcgacgggcgcctggtgaaggtcaaggcgccaggggaatacgacgagggcttcgacaaggccacccgcggcctgaagaaggcccgcatcgagagctacaagggcttcgtcttcatcagcctggacgtggccgccgacaattccctggaagacttcctcggcgacgccaggatcttcttcgacatgatggtcgcccagtcccccgacggtgagctggaggtgctgccgggcaagtccgcctacacctacgacggcaactggaagctgcagaacgagaacggcctggacggttatcacgtcagcaccgtgcactacaactacgtggccacggtgcagcaccgccagcaggtcaacgccgacagcggcgccggcagcagcgccaccctggactacagcaagctcggcgccggcgacgccagcaccgacgatggctggttcgccttcgagaacggccacagcgtgctgttcagcgaaatgcccaacccggcggtgcgctccggctacgccagcatcatgccgcgcctggtggccgagcacggccaggccaaggccgagtggatgatgcaccgcctgcgcaacctgaacatctaccccagcctgttcttcctcgaccagatcagttcccagttgcggatcatccgccccatcgcctggaacaagaccgagatcatcagccagtgcatcggggtcaagggcgagagcgacgccgaccgggaaaaccgcatccgccagttcgaggatttcttcaacgtctcgggcatgggcacgccggacgacctggtggagttccgcgaggcccagcgcggcttccaggggcgcctggagcgctggagcgacatctcccgcgggcgccagcattgggtcagcgggcccaccgccaacagcgagaacctgggcatccagccggccatgaccggcaccgagatcacccatgaggggctgtacgtcaaccagcaccgcaactggcagaaattcctcctcgacggcctgcagcgccggcccctgaaactgcgtgaggtgtga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J331025_sequence/1
type: Sequence
persistent identity: BBa J331025 sequence
display id: BBa_J331025_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaccgacaagtcgaatgtggaattttccgagcgcgaggcgctgttcttccactcgacgggacgccccggcaagatcgagatcatcgcctcgaagccgatggcgacccagcgcgacctcagcctcgcctactcgccgggcgtcgcggtgccggtgcgcgccatcgcgaaagacccggcgaccgcctatgactataccgccaagggcaatctggtcgcggtgatctccaacggcacggcgatcctggggctgggcaatctgggcgcgctggcgtccaagccggtgatggagggcaagtcggtcctcttcaagcgcttcgccgacgtcgacagcatcgacatcgaactcaagaccgaggatgtcgacaagttcatcgacgcggtcgagctgatggagccgaccttcggcggcatcaacctggaggacatcaaggccccggaatgcttcatcatcgaacagacgctgaaggagcggatgaacattccggtcttccatgacgaccagcacggcaccgcgatcatcgcggcggcgggcgtcatcaacgcggcgatgctgaccggccgcgagatgaaggacatgaaggtggtggtgaacggcgcgggcgcggcgtccatctcctgcaccgaactgatcaaggcgctgggcgtgcagcatgacaatgtgatcatgtgcgacagcaagggcgtgatctaccagggccgcaccgagggcatgaaccagtggaagtcggcccatgcggtgaagaccgacgcccgttcgctggccgaagcggtgaagggcgcggacgtgttcctgggcctctccgtcgccggtgcgatgacgcaggacatggtgaagtccatggcggacaagccgatcatcttcgccatggcgaaccccgacccggaaatcgcgccgcccgacgccaaggcggtgcggcccgacgccatcgtcgccacgggccggtcggactatcccaaccaggtcaacaatgtgctgggcttccccttcatcttccgcggcgcgctggacgtgcgggccaccgggatcaacgaggcgatgaagctggccgccgccaaggccatcgccgaactggcgcgggaacaggtgccggaggaagtcgccaaggcctatggccgctcgcacagcttcggcccggactatatcatccccgcgcccttcgacccgcgcctgatggaggtcgtgccctccgccgtcgcgcaggcggcgatggagaccggcgtcgcgcagcggcccatcgcggacatggcggcctatcgccaatccttgaaggggcggctcaacccgaccacctccgtcctcaccaccgcctatgaggtggcgaaggccaatcccaagcgcgtcgtcttcgccgaggcggaagaggaagtagtgctgcgcgccgccatccagttccgcacccttggctatggcatccccgtgctggtcgggcgcgcccatgtctatgacaagctcaaggaactgggcgtggaggatccggaaagctacgaactgcacaacagcgtcaactcgccgctggtgccggaaatggtggacacgctctacaagcggctccagcgccggggctatctgcgccgcgattgcgagcggatggtcaatcgcgaccgtaacatcttcggctcgctgctggtgaagatgggccatgccgacgcgatgctgaccggcatgacccgtcccttcggacagacgctgcgcgaagtgaagcgggtgctggacccggcggcgggccgcacccccttcggcatccatgtgctggtgacgaaggacaagaccgtgttcctggccgacaccacggtgaacgagcgtcccacggcccaggaactggccgacatcgcggaaggtacggtggcggtcgcccggcgcatgggccatgatccgcgcgtcgcattcctgtcctattccaatttcggcaacccgccgggcggcttcctggaaaatgtccgcgacgcggtgaaggtgctggaagaccgcgaggtcgacttcgaattcgaaggggagat…
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z