LOCUS BBa_J331028 7029 bp DNA linear 17-Dec-2024 DEFINITION Enzymatic Pathway for Desulfurization ACCESSION BBa_J331028 VERSION BBa_J331028.1 FEATURES Location/Qualifiers CDS 1..88 /label=end of LacI coding region (inactive) misc_feature 1..200 /label=BBa_R0010 misc_feature 89..126 /label=CAP binding site promoter 137..142 /label=-35 promoter 161..166 /label=-10 misc_feature 166..200 /label=LacI binding site misc_feature 173..173 /label=start promoter 1..200 /label=BBa_R0010 misc_feature 213..216 /label=conserved RBS 209..220 /label=BBa_B0034 CDS 227..1324 /label=DszB misc_feature 227..229 /label=start misc_feature 413..415 /label=Y63F misc_feature 565..565 /label=G->A misc_feature 1252..1252 /label=G->A misc_feature 1322..1324 /label=stop CDS 227..1324 /label=BBa_K902052 misc_feature 1337..1340 /label=conserved RBS 1333..1344 /label=BBa_B0034 CDS 1351..2604 /label=BBa_K804005 misc_feature 2617..2620 /label=conserved RBS 2613..2624 /label=BBa_B0034 misc_feature 2631..2633 /label=start misc_feature 3269..3269 /label=G->A misc_feature 3308..3308 /label=G->A misc_feature 3641..3641 /label=G->A misc_feature 3896..3896 /label=G->A CDS 2631..3992 /label=DszA misc_feature 3990..3992 /label=stop CDS 2631..3992 /label=BBa_K902050 CDS 4001..4088 /label=end of LacI coding region (inactive) misc_feature 4001..4200 /label=BBa_R0010 misc_feature 4089..4126 /label=CAP binding site promoter 4137..4142 /label=-35 promoter 4161..4166 /label=-10 misc_feature 4166..4200 /label=LacI binding site misc_feature 4173..4173 /label=start promoter 4001..4200 /label=BBa_R0010 misc_feature 4213..4216 /label=conserved RBS 4209..4220 /label=BBa_B0034 misc_feature 4227..4229 /label=start CDS 4227..4739 /label=HpaC misc_feature 4737..4739 /label=stop CDS 4227..4739 /label=BBa_K902057 misc_feature 4752..4755 /label=conserved RBS 4748..4759 /label=BBa_B0034 misc_feature 4963..4963 /label=C->T CDS 4766..6982 /label=BBa_K137068 misc_feature 6991..7029 /label=B1006 stem_loop 7000..7021 /label=modified thr terminator polyA_site 7022..7029 /label=PolyA polyA_site 6991..6999 /label=PolyA terminator 6991..7029 /label=BBa_B1006 ORIGIN 1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc 121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga 181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatga caagccgcgt 241 cgaccccgca aaccccggtt cagaactcga ttccgccatc cgcgacacac tgacctacag 301 caactgcccg gtacccaacg ctctgctcac ggcatcggaa tcgggcttcc tcgacgccgc 361 cggcatcgaa ctcgacgtcc tcagcggcca gcagggcacg gttcatttca ccttcgacca 421 gcctgcctac acccgttttg ggggtgagat cccgccactg ctcagcgagg ggttgcgggc 481 acctgggcgc acgcgtctac tcggcatcac cccgctcttg gggcgccagg gcttctttgt 541 ccgcgacgac agcccgatca cagcagccgc cgaccttgcc ggacgtcgaa tcggcgtctc 601 ggcctcggca attcgcatcc tgcgcggcca gctgggcgac tacctcgagt tggatccctg 661 gcggcaaacg ctggtagcgc tgggctcgtg ggaggcgcgc gccttgttgc acacccttga 721 gcacggtgaa ctgggtgtgg acgacgtcga gctggtgccg atcagcagtc ctggtgtcga 781 tgttcccgct gagcagctcg aagaatcggc gaccgtcaag ggtgcggacc tctttcccga 841 tgtcgcccgc ggtcaggccg cggtgttggc cagcggagac gttgacgccc tgtacagttg 901 gctgccctgg gccggggagt tgcaagccac cggggcccgc ccagtggtgg atctcggcct 961 cgatgagcgc aatgcctacg ccagtgtgtg gacggtcagc agcgggctgg ttcgccagcg 1021 acctggcctt gttcaacgac tggtcgacgc ggccgtcgac gccgggctgt gggcacgcga 1081 tcattccgac gcggtgacca gcctgcacgc cgcgaacctg ggcgtatcga ccggagcagt 1141 aggccagggc ttcggcgccg acttccagca gcgtctggtt ccacgcctgg atcacgacgc 1201 cctcgccctc ctggagcgca cacagcaatt cctgctcacc aacaacttgc tacaggaacc 1261 cgtcgccctc gatcagtggg cggctccgga atttctgaac aacagcctca atcgccaccg 1321 atagtactag agaaagagga gaaatactag atgacactgt cacctgaaaa gcagcacgtt 1381 cgaccacgcg acgccgccga caacgatccc gtcgcggttg cccgtgggct agccgaaaag 1441 tggcgagcca ccgccgtcga gcgtgatcgc gccgggggtt cggcaacagc cgagcgcgaa 1501 gacctgcgcg cgagcgcgct gctgtcgctc ctcgtcccgc gcgaatacgg cggctggggc 1561 gcagactggc ccaccgccat cgaggtcgtc cgcgaaatcg cggcagccga tggatctttg 1621 ggacacctgt tcggatacca cctcaccaac gccccgatga tcgaactgat cggctcgcag 1681 gaacaagaag aacacctgta cacccagatc gcgcagaaca actggtggac cggaaatgcc 1741 tccagcgaga acaacagcca cgtgctggac tggaaggtca gcgccacccc gaccgaagac 1801 ggcggctacg tgctcaatgg cacgaagcac ttctacagcg gcgccaaggg gtcggacctg 1861 ctgttcgtgt tcggcgtcgt ccaggatgat tctccgcagc agggtgcgat cattgctgcc 1921 gctatcccga catcgcgggc tggcgttacg cccaacgacg actgggccgc catcggcatg 1981 cggcagaccg acagcggttc cacggacttc cacaacgtca aggtcgagcc tgacgaagtg 2041 ctgggcgcgc ccaacgcctt cgttctcgcc ttcatacaat ccgagcgcgg cagcctcttc 2101 cggcccatag cgcaattgat cttcgccaac gtctatctgg ggatcgcgca cggcgcactc 2161 gatgccgcca gggagtacac ccgtacccag gcgaggccct ggacaccggc cggtattcaa 2221 caggcaaccg aggatcccta caccatccgc tcctacggtg agttcaccat cgcattgcag 2281 ggagctgacg ccgccgcccg tgaagcggcc cacctgctac agacggtgtg ggacaagggc 2341 gacgcgctca cccccgagga ccgcggcgaa ctgatggtga aggtctcggg agtcaaagcg 2401 ttggccacca acgccgccct caacatcagc agcggcgtct tcgaggtgat cggcgcgcgc 2461 ggaacacatc ccaggtacgg tttcgaccgc ttctggcgca acgtgcgcac ccactccctg 2521 cacgacccgg tgtcctacaa gatcgccgac gtcggcaagc acaccttgaa cggtcaatac 2581 ccgattcccg gcttcacctc ctgatactag agaaagagga gaaatactag atgactcaac 2641 aacgacaaat gcatctggcc ggtttcttct cggccggcaa tgtgactcat gcacatgggg 2701 cgtggcggca cacggacgcg tcgaatgact ttctgtcggg gaagtactac caacacatcg 2761 cccgtactct ggagcgcggc aagttcgatc tgttgtttct gcctgacggg ttggccgtcg 2821 aggacagcta cggggacaac ctggacaccg gtgtcggcct gggcgggcag ggtgcagtcg 2881 ccttggagcc ggccagtgtg gtcgcaacca tggccgcggt gaccgagcac ctgggtcttg 2941 gggcaaccat ttcggcgacc tactatcccc cgtatcacgt tgctcgggtg ttcgcgacgc 3001 tcgatcagtt gtcagggggt cgggtgtcct ggaacgtcgt cacctcgctc aacgacgctg 3061 aagcgcgcaa cttcggcatt aatcagcatc tggaacacga cgcccgctat gaccgcgccg 3121 atgagttctt ggaagcggtc aagaaactct ggaacagctg ggacgaggac gccctcgtgc 3181 tggacaaggc ggccggcgtg ttcgccgatc ccgcgaaggt gcactacgtc gatcaccacg 3241 gggagtggct gaatgtgcgc ggacctctac aggtaccgcg ttcacctcag ggtgagccgg 3301 tgatcctaca ggccggcctg tcgccccggg gtcggcgctt cgccgggaag tgggccgagg 3361 ccgtcttcag tcttgcaccc aacctcgagg tgatgcaggc cacctaccag ggcatcaaag 3421 ccgaggtcga cgctgcgggg cgcgatcccg atcagacgaa aatcttcacc gccgtgatgc 3481 cggtactcgg cgaaagccag gcggtggcac aggaacgact ggaatatctc aacagtctgg 3541 tccatccgga agtgggactg tcgacgctat ccagtcacac cggcatcaac ctggcggcgt 3601 accctctcga cactccgatc aaggacatcc tgcgggatct acaggatcgg aatgtcccga 3661 cgcaactgca catgttcgcc gccgcaacgc acagcgaaga gctcacgctg gcggaaatgg 3721 gtcggcgcta tggaaccaac gtggggttcg ttcctcagtg ggccggtacc ggggagcaga 3781 tcgctgacga gctgatccgc cacttcgagg gcggcgccgc ggatggtttc atcatctctc 3841 cggccttcct gccgggctcc tacgacgagt tcgtcgacca ggtggttccg gttctacagg 3901 atcgcggcta cttccgcacc gagtaccagg gcaacactct gcgcgaccac ttgggtctgc 3961 gcgtaccaca actgcaagga caaccttcat gatactagag caatacgcaa accgcctctc 4021 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg 4081 ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta 4141 cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca 4201 tactagagaa agaggagaaa tactagatgc aattagatga acaacgcctg cgctttcgtg 4261 acgcaatggc cagcctgtcg gcagcggtaa atattatcac caccgagggc gacgccggac 4321 aatgcgggat tacggcaacg gccgtctgct cggtcacgga tacaccacca tcgctgatgg 4381 tgtgcattaa cgccaacagt gcgatgaacc cggtttttca gggcaacggt aagttgtgcg 4441 tcaacgtcct caaccatgag caggaactga tggcacgcca cttcgcgggc atgacaggca 4501 tggcgatgga agagcgtttt agcctctcat gctggcaaaa aggtccgctg gcgcagccgg 4561 tgctaaaagg ttcgctggcc agtcttgaag gtgagatccg cgatgtgcag gcaattggca 4621 cacatctggt gtatctggtg gagattaaaa acatcatcct cagtgcagaa ggtcacggac 4681 ttatctactt taaacgccgt ttccatccgg tgatgctgga aatggaagct gcgatttaat 4741 actagagaaa gaggagaaat actagatgag cacgtcagac gatatccata acaccacagc 4801 cactggcaaa tgcccgttcc atcagggcgg tcacgaccag agtgcggggg cgggcacaac 4861 cactcgcgac tggtggccaa atcaacttcg tgttgacctg ttaaaccaac attctaatcg 4921 ttctaaccca ctgggtgagg actttgacta ccgcaaagaa tttagcaaat tagattacta 4981 cggcctgaaa aaagatctga aagccctgtt gacagaatct caaccgtggt ggccagccga 5041 ctggggcagt tacgccggtc tgtttattcg tatggcctgg cacggcgcgg ggacttaccg 5101 ttcaatcgat ggacgcggtg gcgcgggtcg tggtcagcaa cgttttgcac cgctgaactc 5161 ctggccggat aacgtaagcc tcgataaagc gcgtcgcctg ttgtggccaa tcaaacagaa 5221 atatggtcag aaaatctcct gggccgacct gtttatcctc gcgggtaacg tggcgctaga 5281 aaactccggc ttccgtacct tcggttttgg tgccggtcgt gaagacgtct gggaaccgga 5341 tctggatgtt aactggggtg atgaaaaagc ctggctgact caccgtcatc cggaagcgct 5401 ggcgaaagca ccgctgggtg caaccgagat gggtctgatt tacgttaacc cggaaggccc 5461 ggatcacagc ggcgaaccgc tttctgcggc agcagctatc cgcgcgacct tcggcaacat 5521 gggcatgaac gacgaagaaa ccgtggcgct gattgcgggt ggtcatacgc tgggtaaaac 5581 ccacggtgcc ggtccgacat caaatgtagg tcctgatcca gaagctgcac cgattgaaga 5641 acaaggttta ggttgggcga gcacttacgg cagcggcgtt ggcgcagatg ccattacctc 5701 tggtctggaa gtagtctgga cccagacgcc gacccagtgg agcaactatt tcttcgagaa 5761 cctgttcaag tatgagtggg tacagacccg cagcccggct ggcgcaatcc agttcgaagc 5821 ggtagacgca ccggaaatta tcccggatcc gtttgatccg tcgaagaaac gtaaaccgac 5881 aatgctggtg accgacctga cgctgcgttt tgatcctgag ttcgagaaga tctctcgtcg 5941 tttcctcaac gatccgcagg cgttcaacga agcctttgcc cgtgcctggt tcaaactgac 6001 gcacagggat atggggccga aatctcgcta catcgggccg gaagtgccga aagaagatct 6061 gatctggcaa gatccgctgc cgcagccgat ctacaacccg accgagcagg acattatcga 6121 tctgaaattc gcgattgcgg attctggtct gtctgttagt gagctggtat cggtggcctg 6181 ggcatctgct tctaccttcc gtggtggcga caaacgcggt ggtgccaacg gtgcgcgtct 6241 ggcattaatg ccgcagcgcg actgggatgt gaacgccgca gccgttcgtg ctctgcctgt 6301 tctggagaaa atccagaaag agtctggtaa agcctcgctg gcggatatca tagtgctggc 6361 tggtgtggtt ggtgttgaga aagccgcaag cgccgcaggt ttgagcattc atgtaccgtt 6421 tgcgccgggt cgcgttgatg cgcgtcagga tcagactgac attgagatgt ttgagctgct 6481 ggagccaatt gctgacggtt tccgtaacta tcgcgctcgt ctggacgttt ccaccaccga 6541 gtcactgctg atcgacaaag cacagcaact gacgctgacc gcgccggaaa tgactgcgct 6601 ggtgggcggc atgcgtgtac tgggtgccaa cttcgatggc agcaaaaacg gcgtcttcac 6661 tgaccgcgtt ggcgtattga gcaatgactt cttcgtgaac ttgctggata tgcgttacga 6721 gtggaaagcg accgacgaat cgaaagagct gttcgaaggc cgtgaccgtg aaaccggcga 6781 agtgaaattt acggccagcc gtgcggatct ggtgtttggt tctaactccg tcctgcgtgc 6841 ggtggcggaa gtttacgcca gtagcgatgc ccacgagaag tttgttaaag acttcgtggc 6901 ggcatgggtg aaagtgatga acctcgaccg tttcgacctg ctggctgcta acgacgaaaa 6961 ctacgctctg gctgcttaat aatactagag aaaaaaaaac cccgcccctg acagggcggg 7021 gtttttttt //