BBa_K902052 1 dszB HBPS desulfinase (DszB) from R. erythropolis (mutated for improved activity) 2012-09-30T11:00:00Z 2015-05-08T01:13:43Z Amplified from a plasmid in an environmental isolate of Rhodococcus shown to be an active desulfurizer. DszB - HBPS desulfinase which carries out the last step of the 4S pathway, converting 2-hydroxybiphenyl-2-sulfinic acid (HBPS) to 2-hydroxybiphenyl. The sequence has been mutated Y63F to increase enzyme activity. false false _1167_ 0 13399 9 In stock false Mutations of internal PstI and NotI cut sites in order to make biobrick compatible, as well as an added mutation of Y63F in the protein to increase its activity. false Lisa Oberding, Ali Honarmand annotation2205134 1 Y63F range2205134 1 187 189 annotation2205136 1 G->A range2205136 1 1026 1026 annotation2205132 1 DszB range2205132 1 1 1098 annotation2205135 1 G->A range2205135 1 339 339 annotation2205133 1 start range2205133 1 1 3 annotation2211179 1 stop range2211179 1 1096 1098 BBa_R0010 1 LacI promoter (lacI regulated) 2003-01-31T12:00:00Z 2015-05-08T01:14:14Z The Plac insert was PCR'd from the MG1655 strain of E.coli K12. Released HQ 2013 Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) <p> The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG. false true _1_ 0 24 7 In stock false <P> <P><P> LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs. true annotation1961222 1 BBa_R0010 range1961222 1 1 200 annotation1961223 1 CAP binding site range1961223 1 89 126 annotation1961225 1 -10 range1961225 1 161 166 annotation1961227 1 start range1961227 1 173 173 annotation1961221 1 end of LacI coding region (inactive) range1961221 1 1 88 annotation1961226 1 LacI binding site range1961226 1 166 200 annotation1961224 1 -35 range1961224 1 137 142 BBa_J331028 1 BBa_J331028 Enzymatic Pathway for Desulfurization 2016-12-13T12:00:00Z 2016-12-13T09:39:37Z Sulfur: Sulfur or sulphur is a chemical element with symbol S and atomic number 16. It is an abundant, multivalent nonmetal. Under normal conditions, sulfur atoms form cyclic octatomic molecules with chemical formula S8. Elemental sulfur is a bright yellow crystalline solid at room temperature. Chemically, sulfur reacts with all elements except for gold, platinum, iridium, nitrogen, tellurium, iodine and the noble gases. Sulfur third most abundant element in crude oil, when hydrocarbons that have sulfur are burnt they release S02 and S03 gases, sulfur reduces the quality of oil while at the same time polluting the earth. We are going to remove the sulfur in petroleum while keeping the hydrocarbon backbone intact because hurting it will have adverse effects on the petroleums quality. We are going to use a 4s pathway found in Rhodococcus spp, we will remove sulfur from the substrate dibenzothiophene (DBT) and convert it to 2-hydroxybiphenyl (2-HBP), a substrate that does not contain sulfur, we are going to do this without breaking the hydrocarbon backbone false false _1979_2626_ 24251 24251 9 false N/A false Gilles Yvan Buck component2532760 1 BBa_B0034 component2532793 1 BBa_K902057 component2532795 1 BBa_B0034 component2532772 1 BBa_B0034 component2532770 1 BBa_K804005 component2532802 1 BBa_B1006 component2532781 1 BBa_R0010 component2532767 1 BBa_K902052 component2532780 1 BBa_K902050 component2532797 1 BBa_K137068 component2532769 1 BBa_B0034 component2532752 1 BBa_R0010 component2532789 1 BBa_B0034 annotation2532770 1 BBa_K804005 range2532770 1 1351 2604 annotation2532793 1 BBa_K902057 range2532793 1 4227 4739 annotation2532789 1 BBa_B0034 range2532789 1 4209 4220 annotation2532769 1 BBa_B0034 range2532769 1 1333 1344 annotation2532797 1 BBa_K137068 range2532797 1 4766 6982 annotation2532802 1 BBa_B1006 range2532802 1 6991 7029 annotation2532752 1 BBa_R0010 range2532752 1 1 200 annotation2532767 1 BBa_K902052 range2532767 1 227 1324 annotation2532780 1 BBa_K902050 range2532780 1 2631 3992 annotation2532795 1 BBa_B0034 range2532795 1 4748 4759 annotation2532772 1 BBa_B0034 range2532772 1 2613 2624 annotation2532760 1 BBa_B0034 range2532760 1 209 220 annotation2532781 1 BBa_R0010 range2532781 1 4001 4200 BBa_K902057 1 hpaC HpaC- 4-hydroxyphenylacetate 3-monooxygenase reductase subunit 2012-09-30T11:00:00Z 2015-05-08T01:13:43Z Escherichia coli W Recycles FMNH2 utilizing NADH and FMN. false false _1167_ 0 13399 9 In stock false None false Lisa Oberding, Ali Honarmand annotation2211176 1 stop range2211176 1 511 513 annotation2205092 1 HpaC range2205092 1 1 513 annotation2205091 1 start range2205091 1 1 3 BBa_B1006 1 BBa_B1006 Terminator (artificial, large, %T~>90) 2006-08-30T11:00:00Z 2015-08-31T04:07:21Z modified E. coli thr terminator, replaced all A-T pairs in stem with C-G pairs Released HQ 2013 Artificial terminator, estimated %T~>90% *8bp stem, 6nt loop *Bidirectional, estimated reverse %T~>90% false true _41_ 0 745 41 In stock false Bidirectional, with the reverse estimated to be less effective than the forward. Has a polyA tail of 9 residues. true Haiyao Huang annotation1898430 1 PolyA range1898430 1 32 39 annotation1898429 1 modified thr terminator range1898429 1 10 31 annotation1898431 1 PolyA range1898431 1 1 9 annotation1898428 1 B1006 range1898428 1 1 39 BBa_K137068 1 katG katG +LAA 2008-07-30T11:00:00Z 2015-05-08T01:10:09Z Genomic PCR off of DH10B This is one of two catalases from E. coli. It has a C-term LAA ssrA tag. false false _187_ 0 3049 9 It's complicated false Site directed mutagenesis to remove an EcoRI site. false Doug Tischer annotation1969499 1 C->T range1969499 1 198 198 BBa_K804005 1 BBa_K804005 DszC coding gene (from DszABC operon) 2012-09-30T11:00:00Z 2015-05-08T01:13:25Z http://www.ncbi.nlm.nih.gov/nuccore/L37363.1 (GenBank: L37363.1) This is a DszC coding gene from the DszABC operon in the 4S pathway of Rhodococcus erythropolis IGTS8. It encodes one of the three biodesulfurizing enzymes in the DszABC operon. DszC enzymes have been shown to catalyze dibenzothiophene (DBT)to dibenzothiophene-5-oxide (DBTO) in the first reaction and then from DBTO to DBT sulfoxide (DBTO2) in the second reaction, both in the presence of NADH, FMN and flavin reductases. false false _1060_ 0 9774 9 It's complicated false 2 PstI sites were removed using site-directed mutagenesis false Joe Ho BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_K902050 1 dszA DBT-sulfone monooxygenase (DszA) from R. erythropolis 2012-09-30T11:00:00Z 2015-05-08T01:13:43Z Obtained from PCR amplification from a plasmid found in an environmental isolate of R. erythropolis shown to be an active desulfurizer. A monooxygenase enzyme involved in the 4S desulfurization pathway. This protein encoded by this sequence carries out the third step in the pathway, converting DBT-sulfone to 2-hydroxybiphenyl-2-sulfinic acid through addition of an oxygen to the sulfur heteroatom. FMNH2 is consumed by this reaction. false false _1167_ 0 13399 9 It's complicated false 4 internal PstI cut-sites had to be mutated with site-directed mutagenesis. false Lisa Oberding, Ali Honarmand annotation2211180 1 stop range2211180 1 1360 1362 annotation2205051 1 start range2205051 1 1 3 annotation2205054 1 G->A range2205054 1 1011 1011 annotation2205055 1 G->A range2205055 1 1266 1266 annotation2205052 1 G->A range2205052 1 639 639 annotation2205053 1 G->A range2205053 1 678 678 annotation2205093 1 DszA range2205093 1 1 1362 BBa_R0010_sequence 1 caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca BBa_B0034_sequence 1 aaagaggagaaa BBa_K902050_sequence 1 atgactcaacaacgacaaatgcatctggccggtttcttctcggccggcaatgtgactcatgcacatggggcgtggcggcacacggacgcgtcgaatgactttctgtcggggaagtactaccaacacatcgcccgtactctggagcgcggcaagttcgatctgttgtttctgcctgacgggttggccgtcgaggacagctacggggacaacctggacaccggtgtcggcctgggcgggcagggtgcagtcgccttggagccggccagtgtggtcgcaaccatggccgcggtgaccgagcacctgggtcttggggcaaccatttcggcgacctactatcccccgtatcacgttgctcgggtgttcgcgacgctcgatcagttgtcagggggtcgggtgtcctggaacgtcgtcacctcgctcaacgacgctgaagcgcgcaacttcggcattaatcagcatctggaacacgacgcccgctatgaccgcgccgatgagttcttggaagcggtcaagaaactctggaacagctgggacgaggacgccctcgtgctggacaaggcggccggcgtgttcgccgatcccgcgaaggtgcactacgtcgatcaccacggggagtggctgaatgtgcgcggacctctacaggtaccgcgttcacctcagggtgagccggtgatcctacaggccggcctgtcgccccggggtcggcgcttcgccgggaagtgggccgaggccgtcttcagtcttgcacccaacctcgaggtgatgcaggccacctaccagggcatcaaagccgaggtcgacgctgcggggcgcgatcccgatcagacgaaaatcttcaccgccgtgatgccggtactcggcgaaagccaggcggtggcacaggaacgactggaatatctcaacagtctggtccatccggaagtgggactgtcgacgctatccagtcacaccggcatcaacctggcggcgtaccctctcgacactccgatcaaggacatcctgcgggatctacaggatcggaatgtcccgacgcaactgcacatgttcgccgccgcaacgcacagcgaagagctcacgctggcggaaatgggtcggcgctatggaaccaacgtggggttcgttcctcagtgggccggtaccggggagcagatcgctgacgagctgatccgccacttcgagggcggcgccgcggatggtttcatcatctctccggccttcctgccgggctcctacgacgagttcgtcgaccaggtggttccggttctacaggatcgcggctacttccgcaccgagtaccagggcaacactctgcgcgaccacttgggtctgcgcgtaccacaactgcaaggacaaccttcatga BBa_B1006_sequence 1 aaaaaaaaaccccgcccctgacagggcggggtttttttt BBa_J331028_sequence 1 caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatgacaagccgcgtcgaccccgcaaaccccggttcagaactcgattccgccatccgcgacacactgacctacagcaactgcccggtacccaacgctctgctcacggcatcggaatcgggcttcctcgacgccgccggcatcgaactcgacgtcctcagcggccagcagggcacggttcatttcaccttcgaccagcctgcctacacccgttttgggggtgagatcccgccactgctcagcgaggggttgcgggcacctgggcgcacgcgtctactcggcatcaccccgctcttggggcgccagggcttctttgtccgcgacgacagcccgatcacagcagccgccgaccttgccggacgtcgaatcggcgtctcggcctcggcaattcgcatcctgcgcggccagctgggcgactacctcgagttggatccctggcggcaaacgctggtagcgctgggctcgtgggaggcgcgcgccttgttgcacacccttgagcacggtgaactgggtgtggacgacgtcgagctggtgccgatcagcagtcctggtgtcgatgttcccgctgagcagctcgaagaatcggcgaccgtcaagggtgcggacctctttcccgatgtcgcccgcggtcaggccgcggtgttggccagcggagacgttgacgccctgtacagttggctgccctgggccggggagttgcaagccaccggggcccgcccagtggtggatctcggcctcgatgagcgcaatgcctacgccagtgtgtggacggtcagcagcgggctggttcgccagcgacctggccttgttcaacgactggtcgacgcggccgtcgacgccgggctgtgggcacgcgatcattccgacgcggtgaccagcctgcacgccgcgaacctgggcgtatcgaccggagcagtaggccagggcttcggcgccgacttccagcagcgtctggttccacgcctggatcacgacgccctcgccctcctggagcgcacacagcaattcctgctcaccaacaacttgctacaggaacccgtcgccctcgatcagtgggcggctccggaatttctgaacaacagcctcaatcgccaccgatagtactagagaaagaggagaaatactagatgacactgtcacctgaaaagcagcacgttcgaccacgcgacgccgccgacaacgatcccgtcgcggttgcccgtgggctagccgaaaagtggcgagccaccgccgtcgagcgtgatcgcgccgggggttcggcaacagccgagcgcgaagacctgcgcgcgagcgcgctgctgtcgctcctcgtcccgcgcgaatacggcggctggggcgcagactggcccaccgccatcgaggtcgtccgcgaaatcgcggcagccgatggatctttgggacacctgttcggataccacctcaccaacgccccgatgatcgaactgatcggctcgcaggaacaagaagaacacctgtacacccagatcgcgcagaacaactggtggaccggaaatgcctccagcgagaacaacagccacgtgctggactggaaggtcagcgccaccccgaccgaagacggcggctacgtgctcaatggcacgaagcacttctacagcggcgccaaggggtcggacctgctgttcgtgttcggcgtcgtccaggatgattctccgcagcagggtgcgatcattgctgccgctatcccgacatcgcgggctggcgttacgcccaacgacgactgggccgccatcggcatgcggcagaccgacagcggttccacggacttccacaacgtcaaggtcgagcctgacgaagtgctgggcgcgcccaacgccttcgttctcgccttcatacaatccgagcgcggcagcctcttccggcccatagcgcaattgatcttcgccaacgtctatctggggatcgcgcacggcgcactcgatgccgccagggagtacacccgtacccaggcgaggccctggacaccggccggtattcaacaggcaaccgaggatccctacaccatccgctcctacggtgagttcaccatcgcattgcagggagctgacgccgccgcccgtgaagcggcccacctgctacagacggtgtgggacaagggcgacgcgctcacccccgaggaccgcggcgaactgatggtgaaggtctcgggagtcaaagcgttggccaccaacgccgccctcaacatcagcagcggcgtcttcgaggtgatcggcgcgcgcggaacacatcccaggtacggtttcgaccgcttctggcgcaacgtgcgcacccactccctgcacgacccggtgtcctacaagatcgccgacgtcggcaagcacaccttgaacggtcaatacccgattcccggcttcacctcctgatactagagaaagaggagaaatactagatgactcaacaacgacaaatgcatctggccggtttcttctcggccggcaatgtgactcatgcacatggggcgtggcggcacacggacgcgtcgaatgactttctgtcggggaagtactaccaacacatcgcccgtactctggagcgcggcaagttcgatctgttgtttctgcctgacgggttggccgtcgaggacagctacggggacaacctggacaccggtgtcggcctgggcgggcagggtgcagtcgccttggagccggccagtgtggtcgcaaccatggccgcggtgaccgagcacctgggtcttggggcaaccatttcggcgacctactatcccccgtatcacgttgctcgggtgttcgcgacgctcgatcagttgtcagggggtcgggtgtcctggaacgtcgtcacctcgctcaacgacgctgaagcgcgcaacttcggcattaatcagcatctggaacacgacgcccgctatgaccgcgccgatgagttcttggaagcggtcaagaaactctggaacagctgggacgaggacgccctcgtgctggacaaggcggccggcgtgttcgccgatcccgcgaaggtgcactacgtcgatcaccacggggagtggctgaatgtgcgcggacctctacaggtaccgcgttcacctcagggtgagccggtgatcctacaggccggcctgtcgccccggggtcggcgcttcgccgggaagtgggccgaggccgtcttcagtcttgcacccaacctcgaggtgatgcaggccacctaccagggcatcaaagccgaggtcgacgctgcggggcgcgatcccgatcagacgaaaatcttcaccgccgtgatgccggtactcggcgaaagccaggcggtggcacaggaacgactggaatatctcaacagtctggtccatccggaagtgggactgtcgacgctatccagtcacaccggcatcaacctggcggcgtaccctctcgacactccgatcaaggacatcctgcgggatctacaggatcggaatgtcccgacgcaactgcacatgttcgccgccgcaacgcacagcgaagagctcacgctggcggaaatgggtcggcgctatggaaccaacgtggggttcgttcctcagtgggccggtaccggggagcagatcgctgacgagctgatccgccacttcgagggcggcgccgcggatggtttcatcatctctccggccttcctgccgggctcctacgacgagttcgtcgaccaggtggttccggttctacaggatcgcggctacttccgcaccgagtaccagggcaacactctgcgcgaccacttgggtctgcgcgtaccacaactgcaaggacaaccttcatgatactagagcaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatgcaattagatgaacaacgcctgcgctttcgtgacgcaatggccagcctgtcggcagcggtaaatattatcaccaccgagggcgacgccggacaatgcgggattacggcaacggccgtctgctcggtcacggatacaccaccatcgctgatggtgtgcattaacgccaacagtgcgatgaacccggtttttcagggcaacggtaagttgtgcgtcaacgtcctcaaccatgagcaggaactgatggcacgccacttcgcgggcatgacaggcatggcgatggaagagcgttttagcctctcatgctggcaaaaaggtccgctggcgcagccggtgctaaaaggttcgctggccagtcttgaaggtgagatccgcgatgtgcaggcaattggcacacatctggtgtatctggtggagattaaaaacatcatcctcagtgcagaaggtcacggacttatctactttaaacgccgtttccatccggtgatgctggaaatggaagctgcgatttaatactagagaaagaggagaaatactagatgagcacgtcagacgatatccataacaccacagccactggcaaatgcccgttccatcagggcggtcacgaccagagtgcgggggcgggcacaaccactcgcgactggtggccaaatcaacttcgtgttgacctgttaaaccaacattctaatcgttctaacccactgggtgaggactttgactaccgcaaagaatttagcaaattagattactacggcctgaaaaaagatctgaaagccctgttgacagaatctcaaccgtggtggccagccgactggggcagttacgccggtctgtttattcgtatggcctggcacggcgcggggacttaccgttcaatcgatggacgcggtggcgcgggtcgtggtcagcaacgttttgcaccgctgaactcctggccggataacgtaagcctcgataaagcgcgtcgcctgttgtggccaatcaaacagaaatatggtcagaaaatctcctgggccgacctgtttatcctcgcgggtaacgtggcgctagaaaactccggcttccgtaccttcggttttggtgccggtcgtgaagacgtctgggaaccggatctggatgttaactggggtgatgaaaaagcctggctgactcaccgtcatccggaagcgctggcgaaagcaccgctgggtgcaaccgagatgggtctgatttacgttaacccggaaggcccggatcacagcggcgaaccgctttctgcggcagcagctatccgcgcgaccttcggcaacatgggcatgaacgacgaagaaaccgtggcgctgattgcgggtggtcatacgctgggtaaaacccacggtgccggtccgacatcaaatgtaggtcctgatccagaagctgcaccgattgaagaacaaggtttaggttgggcgagcacttacggcagcggcgttggcgcagatgccattacctctggtctggaagtagtctggacccagacgccgacccagtggagcaactatttcttcgagaacctgttcaagtatgagtgggtacagacccgcagcccggctggcgcaatccagttcgaagcggtagacgcaccggaaattatcccggatccgtttgatccgtcgaagaaacgtaaaccgacaatgctggtgaccgacctgacgctgcgttttgatcctgagttcgagaagatctctcgtcgtttcctcaacgatccgcaggcgttcaacgaagcctttgcccgtgcctggttcaaactgacgcacagggatatggggccgaaatctcgctacatcgggccggaagtgccgaaagaagatctgatctggcaagatccgctgccgcagccgatctacaacccgaccgagcaggacattatcgatctgaaattcgcgattgcggattctggtctgtctgttagtgagctggtatcggtggcctgggcatctgcttctaccttccgtggtggcgacaaacgcggtggtgccaacggtgcgcgtctggcattaatgccgcagcgcgactgggatgtgaacgccgcagccgttcgtgctctgcctgttctggagaaaatccagaaagagtctggtaaagcctcgctggcggatatcatagtgctggctggtgtggttggtgttgagaaagccgcaagcgccgcaggtttgagcattcatgtaccgtttgcgccgggtcgcgttgatgcgcgtcaggatcagactgacattgagatgtttgagctgctggagccaattgctgacggtttccgtaactatcgcgctcgtctggacgtttccaccaccgagtcactgctgatcgacaaagcacagcaactgacgctgaccgcgccggaaatgactgcgctggtgggcggcatgcgtgtactgggtgccaacttcgatggcagcaaaaacggcgtcttcactgaccgcgttggcgtattgagcaatgacttcttcgtgaacttgctggatatgcgttacgagtggaaagcgaccgacgaatcgaaagagctgttcgaaggccgtgaccgtgaaaccggcgaagtgaaatttacggccagccgtgcggatctggtgtttggttctaactccgtcctgcgtgcggtggcggaagtttacgccagtagcgatgcccacgagaagtttgttaaagacttcgtggcggcatgggtgaaagtgatgaacctcgaccgtttcgacctgctggctgctaacgacgaaaactacgctctggctgcttaataatactagagaaaaaaaaaccccgcccctgacagggcggggtttttttt BBa_K902052_sequence 1 atgacaagccgcgtcgaccccgcaaaccccggttcagaactcgattccgccatccgcgacacactgacctacagcaactgcccggtacccaacgctctgctcacggcatcggaatcgggcttcctcgacgccgccggcatcgaactcgacgtcctcagcggccagcagggcacggttcatttcaccttcgaccagcctgcctacacccgttttgggggtgagatcccgccactgctcagcgaggggttgcgggcacctgggcgcacgcgtctactcggcatcaccccgctcttggggcgccagggcttctttgtccgcgacgacagcccgatcacagcagccgccgaccttgccggacgtcgaatcggcgtctcggcctcggcaattcgcatcctgcgcggccagctgggcgactacctcgagttggatccctggcggcaaacgctggtagcgctgggctcgtgggaggcgcgcgccttgttgcacacccttgagcacggtgaactgggtgtggacgacgtcgagctggtgccgatcagcagtcctggtgtcgatgttcccgctgagcagctcgaagaatcggcgaccgtcaagggtgcggacctctttcccgatgtcgcccgcggtcaggccgcggtgttggccagcggagacgttgacgccctgtacagttggctgccctgggccggggagttgcaagccaccggggcccgcccagtggtggatctcggcctcgatgagcgcaatgcctacgccagtgtgtggacggtcagcagcgggctggttcgccagcgacctggccttgttcaacgactggtcgacgcggccgtcgacgccgggctgtgggcacgcgatcattccgacgcggtgaccagcctgcacgccgcgaacctgggcgtatcgaccggagcagtaggccagggcttcggcgccgacttccagcagcgtctggttccacgcctggatcacgacgccctcgccctcctggagcgcacacagcaattcctgctcaccaacaacttgctacaggaacccgtcgccctcgatcagtgggcggctccggaatttctgaacaacagcctcaatcgccaccgatag BBa_K804005_sequence 1 atgacactgtcacctgaaaagcagcacgttcgaccacgcgacgccgccgacaacgatcccgtcgcggttgcccgtgggctagccgaaaagtggcgagccaccgccgtcgagcgtgatcgcgccgggggttcggcaacagccgagcgcgaagacctgcgcgcgagcgcgctgctgtcgctcctcgtcccgcgcgaatacggcggctggggcgcagactggcccaccgccatcgaggtcgtccgcgaaatcgcggcagccgatggatctttgggacacctgttcggataccacctcaccaacgccccgatgatcgaactgatcggctcgcaggaacaagaagaacacctgtacacccagatcgcgcagaacaactggtggaccggaaatgcctccagcgagaacaacagccacgtgctggactggaaggtcagcgccaccccgaccgaagacggcggctacgtgctcaatggcacgaagcacttctacagcggcgccaaggggtcggacctgctgttcgtgttcggcgtcgtccaggatgattctccgcagcagggtgcgatcattgctgccgctatcccgacatcgcgggctggcgttacgcccaacgacgactgggccgccatcggcatgcggcagaccgacagcggttccacggacttccacaacgtcaaggtcgagcctgacgaagtgctgggcgcgcccaacgccttcgttctcgccttcatacaatccgagcgcggcagcctcttccggcccatagcgcaattgatcttcgccaacgtctatctggggatcgcgcacggcgcactcgatgccgccagggagtacacccgtacccaggcgaggccctggacaccggccggtattcaacaggcaaccgaggatccctacaccatccgctcctacggtgagttcaccatcgcattgcagggagctgacgccgccgcccgtgaagcggcccacctgctacagacggtgtgggacaagggcgacgcgctcacccccgaggaccgcggcgaactgatggtgaaggtctcgggagtcaaagcgttggccaccaacgccgccctcaacatcagcagcggcgtcttcgaggtgatcggcgcgcgcggaacacatcccaggtacggtttcgaccgcttctggcgcaacgtgcgcacccactccctgcacgacccggtgtcctacaagatcgccgacgtcggcaagcacaccttgaacggtcaatacccgattcccggcttcacctcctga BBa_K137068_sequence 1 atgagcacgtcagacgatatccataacaccacagccactggcaaatgcccgttccatcagggcggtcacgaccagagtgcgggggcgggcacaaccactcgcgactggtggccaaatcaacttcgtgttgacctgttaaaccaacattctaatcgttctaacccactgggtgaggactttgactaccgcaaagaatttagcaaattagattactacggcctgaaaaaagatctgaaagccctgttgacagaatctcaaccgtggtggccagccgactggggcagttacgccggtctgtttattcgtatggcctggcacggcgcggggacttaccgttcaatcgatggacgcggtggcgcgggtcgtggtcagcaacgttttgcaccgctgaactcctggccggataacgtaagcctcgataaagcgcgtcgcctgttgtggccaatcaaacagaaatatggtcagaaaatctcctgggccgacctgtttatcctcgcgggtaacgtggcgctagaaaactccggcttccgtaccttcggttttggtgccggtcgtgaagacgtctgggaaccggatctggatgttaactggggtgatgaaaaagcctggctgactcaccgtcatccggaagcgctggcgaaagcaccgctgggtgcaaccgagatgggtctgatttacgttaacccggaaggcccggatcacagcggcgaaccgctttctgcggcagcagctatccgcgcgaccttcggcaacatgggcatgaacgacgaagaaaccgtggcgctgattgcgggtggtcatacgctgggtaaaacccacggtgccggtccgacatcaaatgtaggtcctgatccagaagctgcaccgattgaagaacaaggtttaggttgggcgagcacttacggcagcggcgttggcgcagatgccattacctctggtctggaagtagtctggacccagacgccgacccagtggagcaactatttcttcgagaacctgttcaagtatgagtgggtacagacccgcagcccggctggcgcaatccagttcgaagcggtagacgcaccggaaattatcccggatccgtttgatccgtcgaagaaacgtaaaccgacaatgctggtgaccgacctgacgctgcgttttgatcctgagttcgagaagatctctcgtcgtttcctcaacgatccgcaggcgttcaacgaagcctttgcccgtgcctggttcaaactgacgcacagggatatggggccgaaatctcgctacatcgggccggaagtgccgaaagaagatctgatctggcaagatccgctgccgcagccgatctacaacccgaccgagcaggacattatcgatctgaaattcgcgattgcggattctggtctgtctgttagtgagctggtatcggtggcctgggcatctgcttctaccttccgtggtggcgacaaacgcggtggtgccaacggtgcgcgtctggcattaatgccgcagcgcgactgggatgtgaacgccgcagccgttcgtgctctgcctgttctggagaaaatccagaaagagtctggtaaagcctcgctggcggatatcatagtgctggctggtgtggttggtgttgagaaagccgcaagcgccgcaggtttgagcattcatgtaccgtttgcgccgggtcgcgttgatgcgcgtcaggatcagactgacattgagatgtttgagctgctggagccaattgctgacggtttccgtaactatcgcgctcgtctggacgtttccaccaccgagtcactgctgatcgacaaagcacagcaactgacgctgaccgcgccggaaatgactgcgctggtgggcggcatgcgtgtactgggtgccaacttcgatggcagcaaaaacggcgtcttcactgaccgcgttggcgtattgagcaatgacttcttcgtgaacttgctggatatgcgttacgagtggaaagcgaccgacgaatcgaaagagctgttcgaaggccgtgaccgtgaaaccggcgaagtgaaatttacggccagccgtgcggatctggtgtttggttctaactccgtcctgcgtgcggtggcggaagtttacgccagtagcgatgcccacgagaagtttgttaaagacttcgtggcggcatgggtgaaagtgatgaacctcgaccgtttcgacctgctggctgctaacgacgaaaactacgctctggctgcttaataa BBa_K902057_sequence 1 atgcaattagatgaacaacgcctgcgctttcgtgacgcaatggccagcctgtcggcagcggtaaatattatcaccaccgagggcgacgccggacaatgcgggattacggcaacggccgtctgctcggtcacggatacaccaccatcgctgatggtgtgcattaacgccaacagtgcgatgaacccggtttttcagggcaacggtaagttgtgcgtcaacgtcctcaaccatgagcaggaactgatggcacgccacttcgcgggcatgacaggcatggcgatggaagagcgttttagcctctcatgctggcaaaaaggtccgctggcgcagccggtgctaaaaggttcgctggccagtcttgaaggtgagatccgcgatgtgcaggcaattggcacacatctggtgtatctggtggagattaaaaacatcatcctcagtgcagaaggtcacggacttatctactttaaacgccgtttccatccggtgatgctggaaatggaagctgcgatttaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z