BBa_J331028
type: ComponentDefinition
persistent identity: BBa J331028
display id: BBa_J331028
version: 1
was generated by: iGEM to SBOL conversion
description: Enzymatic Pathway for Desulfurization
created: 2016-12-13T12:00:00Z
modified: 2016-12-13T09:39:37Z
mutable provenance: Sulfur: Sulfur or sulphur is a chemical element with symbol S and atomic number 16. It is an abundant, multivalent nonmetal. Under normal conditions, sulfur atoms form cyclic octatomic molecules with chemical formula S8. Elemental sulfur is a bright yellow crystalline solid at room temperature. Chemically, sulfur reacts with all elements except for gold, platinum, iridium, nitrogen, tellurium, iodine and the noble gases.
owned by: james
owned by: myers
mutable description: Sulfur third most abundant element in crude oil, when hydrocarbons that have sulfur are burnt they release S02 and S03 gases, sulfur reduces the quality of oil while at the same time polluting the earth. We are going to remove the sulfur in petroleum while keeping the hydrocarbon backbone intact because hurting it will have adverse effects on the petroleums quality. We are going to use a 4s pathway found in Rhodococcus spp, we will remove sulfur from the substrate dibenzothiophene (DBT) and convert it to 2-hydroxybiphenyl (2-HBP), a substrate that does not contain sulfur, we are going to do this without breaking the hydrocarbon backbone
discontinued: false
dominant: false
group u list: _1979_2626_
m user id: 24251
owner id: 24251
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: N/A
star: false
creator: Gilles Yvan Buck
type: DnaRegion
role: partType/Temporary
role: SO:0000110
component: BBa_R0010
component: BBa_K902050
component: BBa_B0034
component: BBa_K804005
component: BBa_K902052
component: BBa_B1006
component: BBa_K137068
component: BBa_K902057
sequence annotation: BBa_R0010
sequence annotation: BBa_B0034
sequence annotation: BBa_K902052
sequence annotation: BBa_K804005
sequence annotation: BBa_K902050
sequence annotation: BBa_K902057
sequence annotation: BBa_K137068
sequence annotation: BBa_B1006
sequence: https://synbiohub.org/public/igem/BBa_J331028_sequence/1

BBa_R0010
type: Component
persistent identity: component2532781
display id: component2532781
version: 1
top level: BBa_J331028
definition: LacI
access: public

BBa_K902050
type: Component
persistent identity: component2532780
display id: component2532780
version: 1
top level: BBa_J331028
definition: dszA
access: public

BBa_B0034
type: Component
persistent identity: component2532772
display id: component2532772
version: 1
top level: BBa_J331028
definition: BBa_B0034
access: public

BBa_K804005
type: Component
persistent identity: component2532770
display id: component2532770
version: 1
top level: BBa_J331028
definition: BBa_K804005
access: public

BBa_B0034
type: Component
persistent identity: component2532769
display id: component2532769
version: 1
top level: BBa_J331028
definition: BBa_B0034
access: public

BBa_K902052
type: Component
persistent identity: component2532767
display id: component2532767
version: 1
top level: BBa_J331028
definition: dszB
access: public

BBa_B0034
type: Component
persistent identity: component2532760
display id: component2532760
version: 1
top level: BBa_J331028
definition: BBa_B0034
access: public

BBa_R0010
type: Component
persistent identity: component2532752
display id: component2532752
version: 1
top level: BBa_J331028
definition: LacI
access: public

BBa_B1006
type: Component
persistent identity: component2532802
display id: component2532802
version: 1
top level: BBa_J331028
definition: BBa_B1006
access: public

BBa_K137068
type: Component
persistent identity: component2532797
display id: component2532797
version: 1
top level: BBa_J331028
definition: katG
access: public

BBa_B0034
type: Component
persistent identity: component2532795
display id: component2532795
version: 1
top level: BBa_J331028
definition: BBa_B0034
access: public

BBa_K902057
type: Component
persistent identity: component2532793
display id: component2532793
version: 1
top level: BBa_J331028
definition: hpaC
access: public

BBa_B0034
type: Component
persistent identity: component2532789
display id: component2532789
version: 1
top level: BBa_J331028
definition: BBa_B0034
access: public

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2532752
display id: annotation2532752
version: 1
top level: BBa_J331028
location: https://synbiohub.org/public/igem/BBa_J331028/annotation2532752/range2532752/1
component: BBa_R0010

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type: Range
persistent identity: range2532752
display id: range2532752
version: 1
top level: BBa_J331028
direction: direction/forward
start: 1
end: 200
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2532760
display id: annotation2532760
version: 1
top level: BBa_J331028
location: https://synbiohub.org/public/igem/BBa_J331028/annotation2532760/range2532760/1
component: BBa_B0034

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persistent identity: range2532760
display id: range2532760
version: 1
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direction: direction/reverse
start: 209
end: 220
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BBa_K902052
type: SequenceAnnotation
persistent identity: annotation2532767
display id: annotation2532767
version: 1
top level: BBa_J331028
location: https://synbiohub.org/public/igem/BBa_J331028/annotation2532767/range2532767/1
component: BBa_K902052

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type: Range
persistent identity: range2532767
display id: range2532767
version: 1
top level: BBa_J331028
direction: direction/reverse
start: 227
end: 1324
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2532769
display id: annotation2532769
version: 1
top level: BBa_J331028
location: https://synbiohub.org/public/igem/BBa_J331028/annotation2532769/range2532769/1
component: BBa_B0034

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type: Range
persistent identity: range2532769
display id: range2532769
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top level: BBa_J331028
direction: direction/reverse
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end: 1344
orientation: inline

BBa_K804005
type: SequenceAnnotation
persistent identity: annotation2532770
display id: annotation2532770
version: 1
top level: BBa_J331028
location: https://synbiohub.org/public/igem/BBa_J331028/annotation2532770/range2532770/1
component: BBa_K804005

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type: Range
persistent identity: range2532770
display id: range2532770
version: 1
top level: BBa_J331028
direction: direction/reverse
start: 1351
end: 2604
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2532772
display id: annotation2532772
version: 1
top level: BBa_J331028
location: https://synbiohub.org/public/igem/BBa_J331028/annotation2532772/range2532772/1
component: BBa_B0034

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type: Range
persistent identity: range2532772
display id: range2532772
version: 1
top level: BBa_J331028
direction: direction/reverse
start: 2613
end: 2624
orientation: inline

BBa_K902050
type: SequenceAnnotation
persistent identity: annotation2532780
display id: annotation2532780
version: 1
top level: BBa_J331028
location: https://synbiohub.org/public/igem/BBa_J331028/annotation2532780/range2532780/1
component: BBa_K902050

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type: Range
persistent identity: range2532780
display id: range2532780
version: 1
top level: BBa_J331028
direction: direction/reverse
start: 2631
end: 3992
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2532781
display id: annotation2532781
version: 1
top level: BBa_J331028
location: https://synbiohub.org/public/igem/BBa_J331028/annotation2532781/range2532781/1
component: BBa_R0010

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type: Range
persistent identity: range2532781
display id: range2532781
version: 1
top level: BBa_J331028
direction: direction/forward
start: 4001
end: 4200
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2532789
display id: annotation2532789
version: 1
top level: BBa_J331028
location: https://synbiohub.org/public/igem/BBa_J331028/annotation2532789/range2532789/1
component: BBa_B0034

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type: Range
persistent identity: range2532789
display id: range2532789
version: 1
top level: BBa_J331028
direction: direction/reverse
start: 4209
end: 4220
orientation: inline

BBa_K902057
type: SequenceAnnotation
persistent identity: annotation2532793
display id: annotation2532793
version: 1
top level: BBa_J331028
location: https://synbiohub.org/public/igem/BBa_J331028/annotation2532793/range2532793/1
component: BBa_K902057

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type: Range
persistent identity: range2532793
display id: range2532793
version: 1
top level: BBa_J331028
direction: direction/reverse
start: 4227
end: 4739
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2532795
display id: annotation2532795
version: 1
top level: BBa_J331028
location: https://synbiohub.org/public/igem/BBa_J331028/annotation2532795/range2532795/1
component: BBa_B0034

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type: Range
persistent identity: range2532795
display id: range2532795
version: 1
top level: BBa_J331028
direction: direction/reverse
start: 4748
end: 4759
orientation: inline

BBa_K137068
type: SequenceAnnotation
persistent identity: annotation2532797
display id: annotation2532797
version: 1
top level: BBa_J331028
location: https://synbiohub.org/public/igem/BBa_J331028/annotation2532797/range2532797/1
component: BBa_K137068

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type: Range
persistent identity: range2532797
display id: range2532797
version: 1
top level: BBa_J331028
direction: direction/reverse
start: 4766
end: 6982
orientation: inline

BBa_B1006
type: SequenceAnnotation
persistent identity: annotation2532802
display id: annotation2532802
version: 1
top level: BBa_J331028
location: https://synbiohub.org/public/igem/BBa_J331028/annotation2532802/range2532802/1
component: BBa_B1006

https://synbiohub.org/public/igem/BBa_J331028/annotation2532802/range2532802/1
type: Range
persistent identity: range2532802
display id: range2532802
version: 1
top level: BBa_J331028
direction: direction/reverse
start: 6991
end: 7029
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

LacI
type: ComponentDefinition
persistent identity: BBa R0010
display id: BBa_R0010
version: 1
was generated by: iGEM to SBOL conversion
description: promoter (lacI regulated)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: The Plac insert was PCR'd from the MG1655 strain of E.coli K12.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs.
star: true
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: end of LacI coding region (inactive)
sequence annotation: BBa_R0010
sequence annotation: CAP binding site
sequence annotation: -35
sequence annotation: -10
sequence annotation: LacI binding site
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_R0010_sequence/1

end of LacI coding region (inactive)
type: SequenceAnnotation
persistent identity: annotation1961221
display id: annotation1961221
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
type: Range
persistent identity: range1961221
display id: range1961221
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 88
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation1961222
display id: annotation1961222
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
type: Range
persistent identity: range1961222
display id: range1961222
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 200
orientation: inline

CAP binding site
type: SequenceAnnotation
persistent identity: annotation1961223
display id: annotation1961223
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
type: Range
persistent identity: range1961223
display id: range1961223
version: 1
top level: LacI
direction: direction/forward
start: 89
end: 126
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1961224
display id: annotation1961224
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
type: Range
persistent identity: range1961224
display id: range1961224
version: 1
top level: LacI
direction: direction/forward
start: 137
end: 142
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1961225
display id: annotation1961225
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
type: Range
persistent identity: range1961225
display id: range1961225
version: 1
top level: LacI
direction: direction/forward
start: 161
end: 166
orientation: inline

LacI binding site
type: SequenceAnnotation
persistent identity: annotation1961226
display id: annotation1961226
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
type: Range
persistent identity: range1961226
display id: range1961226
version: 1
top level: LacI
direction: direction/forward
start: 166
end: 200
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1961227
display id: annotation1961227
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
type: Range
persistent identity: range1961227
display id: range1961227
version: 1
top level: LacI
direction: direction/forward
start: 173
end: 173
orientation: inline

dszA
type: ComponentDefinition
persistent identity: BBa K902050
display id: BBa_K902050
version: 1
was generated by: iGEM to SBOL conversion
description: DBT-sulfone monooxygenase (DszA) from R. erythropolis
created: 2012-09-30T11:00:00Z
modified: 2015-05-08T01:13:43Z
mutable provenance: Obtained from PCR amplification from a plasmid found in an environmental isolate of R. erythropolis shown to be an active desulfurizer.
owned by: james
owned by: myers
mutable description: A monooxygenase enzyme involved in the 4S desulfurization pathway. This protein encoded by this sequence carries out the third step in the pathway, converting DBT-sulfone to 2-hydroxybiphenyl-2-sulfinic acid through addition of an oxygen to the sulfur heteroatom. FMNH2 is consumed by this reaction.
discontinued: false
dominant: false
experience: experience/None
group u list: _1167_
m user id: 0
owner id: 13399
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: 4 internal PstI cut-sites had to be mutated with site-directed mutagenesis.
star: false
creator: Lisa Oberding, Ali Honarmand
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: DszA
sequence annotation: start
sequence annotation: G->A
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_K902050_sequence/1

DszA
type: SequenceAnnotation
persistent identity: annotation2205093
display id: annotation2205093
version: 1
top level: dszA
location: https://synbiohub.org/public/igem/BBa_K902050/annotation2205093/range2205093/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K902050/annotation2205093/range2205093/1
type: Range
persistent identity: range2205093
display id: range2205093
version: 1
top level: dszA
direction: direction/forward
start: 1
end: 1362
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2205051
display id: annotation2205051
version: 1
top level: dszA
location: https://synbiohub.org/public/igem/BBa_K902050/annotation2205051/range2205051/1
role: SO:0000318
role: feature/start

https://synbiohub.org/public/igem/BBa_K902050/annotation2205051/range2205051/1
type: Range
persistent identity: range2205051
display id: range2205051
version: 1
top level: dszA
direction: direction/forward
start: 1
end: 3
orientation: inline

G->A
type: SequenceAnnotation
persistent identity: annotation2205052
display id: annotation2205052
version: 1
top level: dszA
location: https://synbiohub.org/public/igem/BBa_K902050/annotation2205052/range2205052/1
role: SO:0001059
role: feature/mutation

https://synbiohub.org/public/igem/BBa_K902050/annotation2205052/range2205052/1
type: Range
persistent identity: range2205052
display id: range2205052
version: 1
top level: dszA
direction: direction/forward
start: 639
end: 639
orientation: inline

G->A
type: SequenceAnnotation
persistent identity: annotation2205053
display id: annotation2205053
version: 1
top level: dszA
location: https://synbiohub.org/public/igem/BBa_K902050/annotation2205053/range2205053/1
role: SO:0001059
role: feature/mutation

https://synbiohub.org/public/igem/BBa_K902050/annotation2205053/range2205053/1
type: Range
persistent identity: range2205053
display id: range2205053
version: 1
top level: dszA
direction: direction/forward
start: 678
end: 678
orientation: inline

G->A
type: SequenceAnnotation
persistent identity: annotation2205054
display id: annotation2205054
version: 1
top level: dszA
location: https://synbiohub.org/public/igem/BBa_K902050/annotation2205054/range2205054/1
role: SO:0001059
role: feature/mutation

https://synbiohub.org/public/igem/BBa_K902050/annotation2205054/range2205054/1
type: Range
persistent identity: range2205054
display id: range2205054
version: 1
top level: dszA
direction: direction/forward
start: 1011
end: 1011
orientation: inline

G->A
type: SequenceAnnotation
persistent identity: annotation2205055
display id: annotation2205055
version: 1
top level: dszA
location: https://synbiohub.org/public/igem/BBa_K902050/annotation2205055/range2205055/1
role: SO:0001059
role: feature/mutation

https://synbiohub.org/public/igem/BBa_K902050/annotation2205055/range2205055/1
type: Range
persistent identity: range2205055
display id: range2205055
version: 1
top level: dszA
direction: direction/forward
start: 1266
end: 1266
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2211180
display id: annotation2211180
version: 1
top level: dszA
location: https://synbiohub.org/public/igem/BBa_K902050/annotation2211180/range2211180/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_K902050/annotation2211180/range2211180/1
type: Range
persistent identity: range2211180
display id: range2211180
version: 1
top level: dszA
direction: direction/forward
start: 1360
end: 1362
orientation: inline

hpaC
type: ComponentDefinition
persistent identity: BBa K902057
display id: BBa_K902057
version: 1
was generated by: iGEM to SBOL conversion
description: HpaC- 4-hydroxyphenylacetate 3-monooxygenase reductase subunit
created: 2012-09-30T11:00:00Z
modified: 2015-05-08T01:13:43Z
mutable provenance: Escherichia coli W
owned by: james
owned by: myers
mutable description: Recycles FMNH2 utilizing NADH and FMN.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1167_
m user id: 0
owner id: 13399
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: None
star: false
creator: Lisa Oberding, Ali Honarmand
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: start
sequence annotation: HpaC
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_K902057_sequence/1

start
type: SequenceAnnotation
persistent identity: annotation2205091
display id: annotation2205091
version: 1
top level: hpaC
location: https://synbiohub.org/public/igem/BBa_K902057/annotation2205091/range2205091/1
role: SO:0000318
role: feature/start

https://synbiohub.org/public/igem/BBa_K902057/annotation2205091/range2205091/1
type: Range
persistent identity: range2205091
display id: range2205091
version: 1
top level: hpaC
direction: direction/forward
start: 1
end: 3
orientation: inline

HpaC
type: SequenceAnnotation
persistent identity: annotation2205092
display id: annotation2205092
version: 1
top level: hpaC
location: https://synbiohub.org/public/igem/BBa_K902057/annotation2205092/range2205092/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K902057/annotation2205092/range2205092/1
type: Range
persistent identity: range2205092
display id: range2205092
version: 1
top level: hpaC
direction: direction/forward
start: 1
end: 513
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2211176
display id: annotation2211176
version: 1
top level: hpaC
location: https://synbiohub.org/public/igem/BBa_K902057/annotation2211176/range2211176/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_K902057/annotation2211176/range2211176/1
type: Range
persistent identity: range2211176
display id: range2211176
version: 1
top level: hpaC
direction: direction/forward
start: 511
end: 513
orientation: inline

BBa_K804005
type: ComponentDefinition
persistent identity: BBa K804005
display id: BBa_K804005
version: 1
was generated by: iGEM to SBOL conversion
description: DszC coding gene (from DszABC operon)
created: 2012-09-30T11:00:00Z
modified: 2015-05-08T01:13:25Z
mutable provenance: http://www.ncbi.nlm.nih.gov/nuccore/L37363.1 (GenBank: L37363.1)
owned by: james
owned by: myers
mutable description: This is a DszC coding gene from the DszABC operon in the 4S pathway of Rhodococcus erythropolis IGTS8. It encodes one of the three biodesulfurizing enzymes in the DszABC operon. DszC enzymes have been shown to catalyze dibenzothiophene (DBT)to dibenzothiophene-5-oxide (DBTO) in the first reaction and then from DBTO to DBT sulfoxide (DBTO2) in the second reaction, both in the presence of NADH, FMN and flavin reductases.
discontinued: false
dominant: false
group u list: _1060_
m user id: 0
owner id: 9774
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: 2 PstI sites were removed using site-directed mutagenesis
star: false
creator: Joe Ho
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K804005_sequence/1

dszB
type: ComponentDefinition
persistent identity: BBa K902052
display id: BBa_K902052
version: 1
was generated by: iGEM to SBOL conversion
description: HBPS desulfinase (DszB) from R. erythropolis (mutated for improved activity)
created: 2012-09-30T11:00:00Z
modified: 2015-05-08T01:13:43Z
mutable provenance: Amplified from a plasmid in an environmental isolate of Rhodococcus shown to be an active desulfurizer.
owned by: james
owned by: myers
mutable description: DszB - HBPS desulfinase which carries out the last step of the 4S pathway, converting 2-hydroxybiphenyl-2-sulfinic acid (HBPS) to 2-hydroxybiphenyl. The sequence has been mutated Y63F to increase enzyme activity.
discontinued: false
dominant: false
experience: experience/None
group u list: _1167_
m user id: 0
owner id: 13399
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Mutations of internal PstI and NotI cut sites in order to make biobrick compatible, as well as an added mutation of Y63F in the protein to increase its activity.
star: false
creator: Lisa Oberding, Ali Honarmand
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: DszB
sequence annotation: start
sequence annotation: Y63F
sequence annotation: G->A
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_K902052_sequence/1

DszB
type: SequenceAnnotation
persistent identity: annotation2205132
display id: annotation2205132
version: 1
top level: dszB
location: https://synbiohub.org/public/igem/BBa_K902052/annotation2205132/range2205132/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K902052/annotation2205132/range2205132/1
type: Range
persistent identity: range2205132
display id: range2205132
version: 1
top level: dszB
direction: direction/forward
start: 1
end: 1098
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2205133
display id: annotation2205133
version: 1
top level: dszB
location: https://synbiohub.org/public/igem/BBa_K902052/annotation2205133/range2205133/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_K902052/annotation2205133/range2205133/1
type: Range
persistent identity: range2205133
display id: range2205133
version: 1
top level: dszB
direction: direction/forward
start: 1
end: 3
orientation: inline

Y63F
type: SequenceAnnotation
persistent identity: annotation2205134
display id: annotation2205134
version: 1
top level: dszB
location: https://synbiohub.org/public/igem/BBa_K902052/annotation2205134/range2205134/1
role: feature/mutation
role: SO:0001059

https://synbiohub.org/public/igem/BBa_K902052/annotation2205134/range2205134/1
type: Range
persistent identity: range2205134
display id: range2205134
version: 1
top level: dszB
direction: direction/forward
start: 187
end: 189
orientation: inline

G->A
type: SequenceAnnotation
persistent identity: annotation2205135
display id: annotation2205135
version: 1
top level: dszB
location: https://synbiohub.org/public/igem/BBa_K902052/annotation2205135/range2205135/1
role: feature/mutation
role: SO:0001059

https://synbiohub.org/public/igem/BBa_K902052/annotation2205135/range2205135/1
type: Range
persistent identity: range2205135
display id: range2205135
version: 1
top level: dszB
direction: direction/forward
start: 339
end: 339
orientation: inline

G->A
type: SequenceAnnotation
persistent identity: annotation2205136
display id: annotation2205136
version: 1
top level: dszB
location: https://synbiohub.org/public/igem/BBa_K902052/annotation2205136/range2205136/1
role: feature/mutation
role: SO:0001059

https://synbiohub.org/public/igem/BBa_K902052/annotation2205136/range2205136/1
type: Range
persistent identity: range2205136
display id: range2205136
version: 1
top level: dszB
direction: direction/forward
start: 1026
end: 1026
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2211179
display id: annotation2211179
version: 1
top level: dszB
location: https://synbiohub.org/public/igem/BBa_K902052/annotation2211179/range2211179/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_K902052/annotation2211179/range2211179/1
type: Range
persistent identity: range2211179
display id: range2211179
version: 1
top level: dszB
direction: direction/forward
start: 1096
end: 1098
orientation: inline

BBa_B1006
type: ComponentDefinition
persistent identity: BBa B1006
display id: BBa_B1006
version: 1
was generated by: iGEM to SBOL conversion
description: Terminator (artificial, large, %T~>90)
created: 2006-08-30T11:00:00Z
modified: 2015-08-31T04:07:21Z
mutable provenance: modified E. coli thr terminator, replaced all A-T pairs in stem with C-G pairs
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Artificial terminator, estimated %T~>90% *8bp stem, 6nt loop *Bidirectional, estimated reverse %T~>90%
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_
m user id: 0
owner id: 745
owning group id: 41
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Bidirectional, with the reverse estimated to be less effective than the forward. Has a polyA tail of 9 residues.
star: true
creator: Haiyao Huang
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: B1006
sequence annotation: PolyA
sequence annotation: modified thr terminator
sequence: https://synbiohub.org/public/igem/BBa_B1006_sequence/1

B1006
type: SequenceAnnotation
persistent identity: annotation1898428
display id: annotation1898428
version: 1
top level: BBa_B1006
location: https://synbiohub.org/public/igem/BBa_B1006/annotation1898428/range1898428/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B1006/annotation1898428/range1898428/1
type: Range
persistent identity: range1898428
display id: range1898428
version: 1
top level: BBa_B1006
direction: direction/forward
start: 1
end: 39
orientation: inline

PolyA
type: SequenceAnnotation
persistent identity: annotation1898431
display id: annotation1898431
version: 1
top level: BBa_B1006
location: https://synbiohub.org/public/igem/BBa_B1006/annotation1898431/range1898431/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B1006/annotation1898431/range1898431/1
type: Range
persistent identity: range1898431
display id: range1898431
version: 1
top level: BBa_B1006
direction: direction/reverse
start: 1
end: 9
orientation: inline

modified thr terminator
type: SequenceAnnotation
persistent identity: annotation1898429
display id: annotation1898429
version: 1
top level: BBa_B1006
location: https://synbiohub.org/public/igem/BBa_B1006/annotation1898429/range1898429/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B1006/annotation1898429/range1898429/1
type: Range
persistent identity: range1898429
display id: range1898429
version: 1
top level: BBa_B1006
direction: direction/forward
start: 10
end: 31
orientation: inline

PolyA
type: SequenceAnnotation
persistent identity: annotation1898430
display id: annotation1898430
version: 1
top level: BBa_B1006
location: https://synbiohub.org/public/igem/BBa_B1006/annotation1898430/range1898430/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B1006/annotation1898430/range1898430/1
type: Range
persistent identity: range1898430
display id: range1898430
version: 1
top level: BBa_B1006
direction: direction/forward
start: 32
end: 39
orientation: inline

katG
type: ComponentDefinition
persistent identity: BBa K137068
display id: BBa_K137068
version: 1
was generated by: iGEM to SBOL conversion
description: katG +LAA
created: 2008-07-30T11:00:00Z
modified: 2015-05-08T01:10:09Z
mutable provenance: Genomic PCR off of DH10B
owned by: james
owned by: myers
mutable description: This is one of two catalases from E. coli. It has a C-term LAA ssrA tag.
discontinued: false
dominant: false
experience: experience/Works
group u list: _187_
m user id: 0
owner id: 3049
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Site directed mutagenesis to remove an EcoRI site.
star: false
creator: Doug Tischer
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: C->T
sequence: https://synbiohub.org/public/igem/BBa_K137068_sequence/1

C->T
type: SequenceAnnotation
persistent identity: annotation1969499
display id: annotation1969499
version: 1
top level: katG
location: https://synbiohub.org/public/igem/BBa_K137068/annotation1969499/range1969499/1
role: SO:0001059
role: feature/mutation

https://synbiohub.org/public/igem/BBa_K137068/annotation1969499/range1969499/1
type: Range
persistent identity: range1969499
display id: range1969499
version: 1
top level: katG
direction: direction/forward
start: 198
end: 198
orientation: inline

https://synbiohub.org/public/igem/BBa_J331028_sequence/1
type: Sequence
persistent identity: BBa J331028 sequence
display id: BBa_J331028_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatgacaagccgcgtcgaccccgcaaaccccggttcagaactcgattccgccatccgcgacacactgacctacagcaactgcccggtacccaacgctctgctcacggcatcggaatcgggcttcctcgacgccgccggcatcgaactcgacgtcctcagcggccagcagggcacggttcatttcaccttcgaccagcctgcctacacccgttttgggggtgagatcccgccactgctcagcgaggggttgcgggcacctgggcgcacgcgtctactcggcatcaccccgctcttggggcgccagggcttctttgtccgcgacgacagcccgatcacagcagccgccgaccttgccggacgtcgaatcggcgtctcggcctcggcaattcgcatcctgcgcggccagctgggcgactacctcgagttggatccctggcggcaaacgctggtagcgctgggctcgtgggaggcgcgcgccttgttgcacacccttgagcacggtgaactgggtgtggacgacgtcgagctggtgccgatcagcagtcctggtgtcgatgttcccgctgagcagctcgaagaatcggcgaccgtcaagggtgcggacctctttcccgatgtcgcccgcggtcaggccgcggtgttggccagcggagacgttgacgccctgtacagttggctgccctgggccggggagttgcaagccaccggggcccgcccagtggtggatctcggcctcgatgagcgcaatgcctacgccagtgtgtggacggtcagcagcgggctggttcgccagcgacctggccttgttcaacgactggtcgacgcggccgtcgacgccgggctgtgggcacgcgatcattccgacgcggtgaccagcctgcacgccgcgaacctgggcgtatcgaccggagcagtaggccagggcttcggcgccgacttccagcagcgtctggttccacgcctggatcacgacgccctcgccctcctggagcgcacacagcaattcctgctcaccaacaacttgctacaggaacccgtcgccctcgatcagtgggcggctccggaatttctgaacaacagcctcaatcgccaccgatagtactagagaaagaggagaaatactagatgacactgtcacctgaaaagcagcacgttcgaccacgcgacgccgccgacaacgatcccgtcgcggttgcccgtgggctagccgaaaagtggcgagccaccgccgtcgagcgtgatcgcgccgggggttcggcaacagccgagcgcgaagacctgcgcgcgagcgcgctgctgtcgctcctcgtcccgcgcgaatacggcggctggggcgcagactggcccaccgccatcgaggtcgtccgcgaaatcgcggcagccgatggatctttgggacacctgttcggataccacctcaccaacgccccgatgatcgaactgatcggctcgcaggaacaagaagaacacctgtacacccagatcgcgcagaacaactggtggaccggaaatgcctccagcgagaacaacagccacgtgctggactggaaggtcagcgccaccccgaccgaagacggcggctacgtgctcaatggcacgaagcacttctacagcggcgccaaggggtcggacctgctgttcgtgttcggcgtcgtccaggatgattctccgcagcagggtgcgatcattgctgccgctatcccgacatcgcgggctggcgttacgcccaacgacgactgggccgccatcggcatgcggcagaccgacagcggttc…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0010_sequence/1
type: Sequence
persistent identity: BBa R0010 sequence
display id: BBa_R0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K902050_sequence/1
type: Sequence
persistent identity: BBa K902050 sequence
display id: BBa_K902050_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgactcaacaacgacaaatgcatctggccggtttcttctcggccggcaatgtgactcatgcacatggggcgtggcggcacacggacgcgtcgaatgactttctgtcggggaagtactaccaacacatcgcccgtactctggagcgcggcaagttcgatctgttgtttctgcctgacgggttggccgtcgaggacagctacggggacaacctggacaccggtgtcggcctgggcgggcagggtgcagtcgccttggagccggccagtgtggtcgcaaccatggccgcggtgaccgagcacctgggtcttggggcaaccatttcggcgacctactatcccccgtatcacgttgctcgggtgttcgcgacgctcgatcagttgtcagggggtcgggtgtcctggaacgtcgtcacctcgctcaacgacgctgaagcgcgcaacttcggcattaatcagcatctggaacacgacgcccgctatgaccgcgccgatgagttcttggaagcggtcaagaaactctggaacagctgggacgaggacgccctcgtgctggacaaggcggccggcgtgttcgccgatcccgcgaaggtgcactacgtcgatcaccacggggagtggctgaatgtgcgcggacctctacaggtaccgcgttcacctcagggtgagccggtgatcctacaggccggcctgtcgccccggggtcggcgcttcgccgggaagtgggccgaggccgtcttcagtcttgcacccaacctcgaggtgatgcaggccacctaccagggcatcaaagccgaggtcgacgctgcggggcgcgatcccgatcagacgaaaatcttcaccgccgtgatgccggtactcggcgaaagccaggcggtggcacaggaacgactggaatatctcaacagtctggtccatccggaagtgggactgtcgacgctatccagtcacaccggcatcaacctggcggcgtaccctctcgacactccgatcaaggacatcctgcgggatctacaggatcggaatgtcccgacgcaactgcacatgttcgccgccgcaacgcacagcgaagagctcacgctggcggaaatgggtcggcgctatggaaccaacgtggggttcgttcctcagtgggccggtaccggggagcagatcgctgacgagctgatccgccacttcgagggcggcgccgcggatggtttcatcatctctccggccttcctgccgggctcctacgacgagttcgtcgaccaggtggttccggttctacaggatcgcggctacttccgcaccgagtaccagggcaacactctgcgcgaccacttgggtctgcgcgtaccacaactgcaaggacaaccttcatga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K902057_sequence/1
type: Sequence
persistent identity: BBa K902057 sequence
display id: BBa_K902057_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgcaattagatgaacaacgcctgcgctttcgtgacgcaatggccagcctgtcggcagcggtaaatattatcaccaccgagggcgacgccggacaatgcgggattacggcaacggccgtctgctcggtcacggatacaccaccatcgctgatggtgtgcattaacgccaacagtgcgatgaacccggtttttcagggcaacggtaagttgtgcgtcaacgtcctcaaccatgagcaggaactgatggcacgccacttcgcgggcatgacaggcatggcgatggaagagcgttttagcctctcatgctggcaaaaaggtccgctggcgcagccggtgctaaaaggttcgctggccagtcttgaaggtgagatccgcgatgtgcaggcaattggcacacatctggtgtatctggtggagattaaaaacatcatcctcagtgcagaaggtcacggacttatctactttaaacgccgtttccatccggtgatgctggaaatggaagctgcgatttaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K804005_sequence/1
type: Sequence
persistent identity: BBa K804005 sequence
display id: BBa_K804005_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgacactgtcacctgaaaagcagcacgttcgaccacgcgacgccgccgacaacgatcccgtcgcggttgcccgtgggctagccgaaaagtggcgagccaccgccgtcgagcgtgatcgcgccgggggttcggcaacagccgagcgcgaagacctgcgcgcgagcgcgctgctgtcgctcctcgtcccgcgcgaatacggcggctggggcgcagactggcccaccgccatcgaggtcgtccgcgaaatcgcggcagccgatggatctttgggacacctgttcggataccacctcaccaacgccccgatgatcgaactgatcggctcgcaggaacaagaagaacacctgtacacccagatcgcgcagaacaactggtggaccggaaatgcctccagcgagaacaacagccacgtgctggactggaaggtcagcgccaccccgaccgaagacggcggctacgtgctcaatggcacgaagcacttctacagcggcgccaaggggtcggacctgctgttcgtgttcggcgtcgtccaggatgattctccgcagcagggtgcgatcattgctgccgctatcccgacatcgcgggctggcgttacgcccaacgacgactgggccgccatcggcatgcggcagaccgacagcggttccacggacttccacaacgtcaaggtcgagcctgacgaagtgctgggcgcgcccaacgccttcgttctcgccttcatacaatccgagcgcggcagcctcttccggcccatagcgcaattgatcttcgccaacgtctatctggggatcgcgcacggcgcactcgatgccgccagggagtacacccgtacccaggcgaggccctggacaccggccggtattcaacaggcaaccgaggatccctacaccatccgctcctacggtgagttcaccatcgcattgcagggagctgacgccgccgcccgtgaagcggcccacctgctacagacggtgtgggacaagggcgacgcgctcacccccgaggaccgcggcgaactgatggtgaaggtctcgggagtcaaagcgttggccaccaacgccgccctcaacatcagcagcggcgtcttcgaggtgatcggcgcgcgcggaacacatcccaggtacggtttcgaccgcttctggcgcaacgtgcgcacccactccctgcacgacccggtgtcctacaagatcgccgacgtcggcaagcacaccttgaacggtcaatacccgattcccggcttcacctcctga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B1006_sequence/1
type: Sequence
persistent identity: BBa B1006 sequence
display id: BBa_B1006_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaaaaaaaaccccgcccctgacagggcggggtttttttt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K137068_sequence/1
type: Sequence
persistent identity: BBa K137068 sequence
display id: BBa_K137068_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgagcacgtcagacgatatccataacaccacagccactggcaaatgcccgttccatcagggcggtcacgaccagagtgcgggggcgggcacaaccactcgcgactggtggccaaatcaacttcgtgttgacctgttaaaccaacattctaatcgttctaacccactgggtgaggactttgactaccgcaaagaatttagcaaattagattactacggcctgaaaaaagatctgaaagccctgttgacagaatctcaaccgtggtggccagccgactggggcagttacgccggtctgtttattcgtatggcctggcacggcgcggggacttaccgttcaatcgatggacgcggtggcgcgggtcgtggtcagcaacgttttgcaccgctgaactcctggccggataacgtaagcctcgataaagcgcgtcgcctgttgtggccaatcaaacagaaatatggtcagaaaatctcctgggccgacctgtttatcctcgcgggtaacgtggcgctagaaaactccggcttccgtaccttcggttttggtgccggtcgtgaagacgtctgggaaccggatctggatgttaactggggtgatgaaaaagcctggctgactcaccgtcatccggaagcgctggcgaaagcaccgctgggtgcaaccgagatgggtctgatttacgttaacccggaaggcccggatcacagcggcgaaccgctttctgcggcagcagctatccgcgcgaccttcggcaacatgggcatgaacgacgaagaaaccgtggcgctgattgcgggtggtcatacgctgggtaaaacccacggtgccggtccgacatcaaatgtaggtcctgatccagaagctgcaccgattgaagaacaaggtttaggttgggcgagcacttacggcagcggcgttggcgcagatgccattacctctggtctggaagtagtctggacccagacgccgacccagtggagcaactatttcttcgagaacctgttcaagtatgagtgggtacagacccgcagcccggctggcgcaatccagttcgaagcggtagacgcaccggaaattatcccggatccgtttgatccgtcgaagaaacgtaaaccgacaatgctggtgaccgacctgacgctgcgttttgatcctgagttcgagaagatctctcgtcgtttcctcaacgatccgcaggcgttcaacgaagcctttgcccgtgcctggttcaaactgacgcacagggatatggggccgaaatctcgctacatcgggccggaagtgccgaaagaagatctgatctggcaagatccgctgccgcagccgatctacaacccgaccgagcaggacattatcgatctgaaattcgcgattgcggattctggtctgtctgttagtgagctggtatcggtggcctgggcatctgcttctaccttccgtggtggcgacaaacgcggtggtgccaacggtgcgcgtctggcattaatgccgcagcgcgactgggatgtgaacgccgcagccgttcgtgctctgcctgttctggagaaaatccagaaagagtctggtaaagcctcgctggcggatatcatagtgctggctggtgtggttggtgttgagaaagccgcaagcgccgcaggtttgagcattcatgtaccgtttgcgccgggtcgcgttgatgcgcgtcaggatcagactgacattgagatgtttgagctgctggagccaattgctgacggtttccgtaactatcgcgctcgtctggacgtttccaccaccgagtcactgctgatcgacaaagcacagcaactgacgctgaccgcgccggaaatgactgcgctggtgggcggcatgcgtgtactgggtgccaacttcgatggcagcaaaaacggcgtcttcactgaccgcgttggcgtattgagcaatgacttcttcgtgaacttgctggatatgcgttacgagtggaaagcgaccgacgaatcgaaagagctgttcgaaggccgtga…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K902052_sequence/1
type: Sequence
persistent identity: BBa K902052 sequence
display id: BBa_K902052_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgacaagccgcgtcgaccccgcaaaccccggttcagaactcgattccgccatccgcgacacactgacctacagcaactgcccggtacccaacgctctgctcacggcatcggaatcgggcttcctcgacgccgccggcatcgaactcgacgtcctcagcggccagcagggcacggttcatttcaccttcgaccagcctgcctacacccgttttgggggtgagatcccgccactgctcagcgaggggttgcgggcacctgggcgcacgcgtctactcggcatcaccccgctcttggggcgccagggcttctttgtccgcgacgacagcccgatcacagcagccgccgaccttgccggacgtcgaatcggcgtctcggcctcggcaattcgcatcctgcgcggccagctgggcgactacctcgagttggatccctggcggcaaacgctggtagcgctgggctcgtgggaggcgcgcgccttgttgcacacccttgagcacggtgaactgggtgtggacgacgtcgagctggtgccgatcagcagtcctggtgtcgatgttcccgctgagcagctcgaagaatcggcgaccgtcaagggtgcggacctctttcccgatgtcgcccgcggtcaggccgcggtgttggccagcggagacgttgacgccctgtacagttggctgccctgggccggggagttgcaagccaccggggcccgcccagtggtggatctcggcctcgatgagcgcaatgcctacgccagtgtgtggacggtcagcagcgggctggttcgccagcgacctggccttgttcaacgactggtcgacgcggccgtcgacgccgggctgtgggcacgcgatcattccgacgcggtgaccagcctgcacgccgcgaacctgggcgtatcgaccggagcagtaggccagggcttcggcgccgacttccagcagcgtctggttccacgcctggatcacgacgccctcgccctcctggagcgcacacagcaattcctgctcaccaacaacttgctacaggaacccgtcgccctcgatcagtgggcggctccggaatttctgaacaacagcctcaatcgccaccgatag
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z