LOCUS       BBa_J33205               353 bp    DNA     linear       29-Nov-2024
DEFINITION  hybrid lambda PR-lac promoter, repressed by lambda cI and lacI, 
            plus lacZ
ACCESSION   BBa_J33205
VERSION     BBa_J33205.1  
FEATURES             Location/Qualifiers
     promoter        9..14
                     /label=PRM -10
     misc_feature    13..29
                     /label=cI binding site OR3
     promoter        32..37
                     /label=PRM -35
     misc_feature    36..52
                     /label=cI binding site OR2
     promoter        50..55
                     /label=PR -35
     misc_feature    60..76
                     /label=cI binding site OR1
     promoter        73..78
                     /label=PR -10
     misc_feature    79..84
                     /label=XhoI fusion site
     misc_feature    85..105
                     /label=LacI binding site
     RBS             112..115
                     /label=rbs
     CDS             123..353
                     /label=lacZ'
ORIGIN
        1 ctccgttaaa tctatcaccg caagggataa atatctaaca acgtgcgcgt tgactatttt
       61 acctctggcg gtgataatct cgagaattgt gagcggataa caatttcaca caggaaacag
      121 ctatgaccat gattacggat tcactggccg tcgttttaca acgtcgtgac tgggaaaacc
      181 ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata
      241 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggc
      301 gctttgcctg gtttccggca ccagaagcgg tgccggaaag ctggctggag tga
//