LOCUS       BBa_J33206               956 bp    DNA     linear       22-Dec-2024
DEFINITION  Bacillus subtilis ars promoter and arsR gene plus E. coli lacZ
ACCESSION   BBa_J33206
VERSION     BBa_J33206.1  
FEATURES             Location/Qualifiers
     misc_feature    98..115
                     /label=ArsR binding site
     promoter        118..123
                     /label=-35
     promoter        142..147
                     /label=-10
     RBS             172..178
                     /label=rbs
     CDS             187..504
                     /label=arsR
     misc_feature    505..510
                     /label=fusion site
     RBS             510..516
                     /label=rbs
     CDS             524..934
                     /label=lacZ'
ORIGIN
        1 ctccgttgct gtagtagcaa agtagcgatg gaggaatata aaaatggaaa acctctatct
       61 ggttacacaa gcttttagcg ggttttacaa ttaatcaaaa taaattgatt tatttgcttg
      121 cattaattta aaaatcatga gtataataaa tacatcaaaa aaaattgatt aaagaaggcg
      181 atatgaatgg atgagacgaa atcagaactg ctacggaaat atgaacaaaa atttaaggct
      241 cttgctgatc agaaacgatt agagatcatg tatgaacttt gtcaaagggg aaaaacctgt
      301 gtttgtgatc tgactgagat ttttgaggtg acgcaatcta aactttctta tcatctaaaa
      361 attttattgg atgccaactt aataacgaaa gagacaaagg gaacatggag ttattatgat
      421 ctaaatgatg aggaagtaaa tggtttatta tcagaagagc tttgttgtat atttagaaaa
      481 aaaggtgaag gagattgctg ctaaactaga ggaggaaaca gctatgacca tgattacgga
      541 ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa
      601 tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga
      661 tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg cgctttgcct ggtttccggc
      721 accagaagcg gtgccggaaa gctggctgga gtgcgatctt cctgaggccg atactgtcgt
      781 cgtcccctca aactggcaga tgcacggcta cgatgcgccc atctacacca acgtgaccta
      841 tcccattacg gtcaatccgc cgtttgttcc cacggagaat ccgacgggtt gttactcgct
      901 cacatttaat gttgatgaaa gctggctgga gtgatactag agtcacacag gaaacc
//