LOCUS       BBa_J33210               600 bp    DNA     linear       22-Dec-2024
DEFINITION  lac promoter and lacZ
ACCESSION   BBa_J33210
VERSION     BBa_J33210.1  
FEATURES             Location/Qualifiers
     misc_feature    248..285
                     /label=CAP binding site
     promoter        297..302
                     /label=-35
     promoter        320..325
                     /label=-10
     misc_feature    332..352
                     /label=LacI binding site
     RBS             359..362
                     /label=RBS
     CDS             370..600
                     /label=lacZ'
     misc_feature    228..228
                     /label=G->C transversion
ORIGIN
        1 ctcatgttat atcccgccgt taaccaccat caaacaggat tttcgcctgc tggggcaaac
       61 cagcgtggac cgcttgctgc aactctctca gggccaggcg gtgaagggca atcagctgtt
      121 gcccgtctca ctggtgaaaa gaaaaaccac cctggcgccc aatacgcaaa ccgcctctcc
      181 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttccccac tggaaagcgg
      241 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac
      301 actttatgct tccggctcgt atgttgtgtg aaattgtgag cggataacaa tttcacacag
      361 gaaacagcta tgaccatgat tacggattca ctggccgtcg ttttacaacg tcgtgactgg
      421 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
      481 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
      541 gaatggcgct ttgcctggtt tccggcacca gaagcggtgc cggaaagctg gctggagtga
//