LOCUS       BBa_J36335              2883 bp    DNA     linear       30-Apr-2024
DEFINITION  Lac+RBS+KaiA+Lac+RBS+KaiC
ACCESSION   BBa_J36335
VERSION     BBa_J36335.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     CDS             229..1086
                     /label=BBa_J36801
     CDS             1095..1182
                     /label=end of LacI coding region (inactive)
     misc_feature    1095..1294
                     /label=BBa_R0010
     misc_feature    1183..1220
                     /label=CAP binding site
     promoter        1231..1236
                     /label=-35
     promoter        1255..1260
                     /label=-10
     misc_feature    1260..1294
                     /label=LacI binding site
     misc_feature    1267..1267
                     /label=start
     promoter        1095..1294
                     /label=BBa_R0010
     misc_feature    1307..1310
                     /label=conserved
     RBS             1303..1314
                     /label=BBa_B0034
     CDS             1321..2883
                     /label=BBa_J36804
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagaggt gctctcgcaa
      241 attgcaatct gcatttgggt ggaatcgacg gcaattttgc aggattgcca gcgggcgctg
      301 tcggccgatc gctatcaact ccaagtctgt gagtctggcg aaatgctctt ggagtatgcc
      361 caaacccatc gtgaccaaat cgactgcctg attttagtgg cagccaatcc cagcttcagg
      421 gcagttgttc agcagctctg ctttgaggga gtggtggtac cagcgattgt cgtaggcgat
      481 cgcgacagtg aggatcccga tgaaccagcc aaagaacagc tctatcacag cgctgaactg
      541 cacctcggta tccatcagct cgagcaattg ccctaccaag ttgatgctgc actggctgaa
      601 tttctgcgct tagccccggt cgagaccatg gccgaccaca tcatgctgat gggggccaac
      661 cacgatcccg agctatcgag ccagcagcgg gacctcgctc agcgactaca agagcgccta
      721 ggctatctcg gggtctacta caagcgtgat cccgatcgct ttctgcgcaa cctacccgcc
      781 tacgaaagcc aaaagctgca ccaagcgatg cagactagct atcgtgaaat cgttttgagc
      841 tatttttcgc cgaatagcaa cctcaaccag agcattgaca acttcgtcaa catggctttc
      901 tttgccgatg ttccagtcac caaagtggta gaaattcaca tggagctgat ggacgagttt
      961 gccaagaagc tccgcgtaga gggacgttca gaggacattt tgctggatta tcggctgact
     1021 ttaattgatg taattgcaca tctttgtgag atgtatcgac ggtctatccc acgagaaacc
     1081 tgatgatact agagcaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
     1141 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
     1201 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt
     1261 gtgtggaatt gtgagcggat aacaatttca cacatactag agaaagagga gaaatactag
     1321 atgacttccg ctgagatgac tagccctaat aataattctg agcaccaagc catcgctaag
     1381 atgcgcacga tgattgaagg ctttgatgat attagtcatg gcggtcttcc aatcgggcga
     1441 tcgaccctcg ttagtggtac ttcaggaacc ggcaagaccc ttttttctat tcaatttctc
     1501 tataacggta ttatcgagtt tgatgagcct ggggttttcg ttactttcga agaaaccccg
     1561 caagatatca ttaaaaacgc ccgtagtttt ggctgggatt tagccaagct ggtcgatgag
     1621 ggcaaactat ttattcttga tgcttcaccc gatccagaag gtcaagaggt tgttggcggc
     1681 ttcgatctct ctgctctgat tgagcggatt aattatgcaa ttcaaaagta tcgagcgcgg
     1741 cgggtttcaa ttgactcggt cacgtccgtt ttccagcaat atgatgcctc ttctgtggtt
     1801 cgccgcgaac tctttcggtt ggtagctcgc ctaaaacaaa ttggggcaac tacggtcatg
     1861 accaccgagc gtatcgagga atatggcccg atcgctcgtt acggtgttga ggaatttgtc
     1921 tccgataacg tcgtgattct ccgcaacgtt ttggaagggg agcgccgtcg ccgcaccctc
     1981 gaaatcctca agctacgtgg caccagccac atgaaagggg aatatccgtt cacgattacg
     2041 gatcatggca tcaatatctt cccgctcggg gcaatgcgcc ttacgcagcg atcgtcgaac
     2101 gtgcgtgttt catctggtgt cgtccgactc gatgaaatgt gtggtggggg cttctttaag
     2161 gactcaatca ttctggcaac tggcgctaca ggcactggta aaactctgtt agttagccgt
     2221 ttcgttgaga atgcttgtgc taacaaagag cgggcgattc tgttcgctta tgaagagtca
     2281 cgagctcagc tgctccgcaa tgcctattca tggggaatgg actttgagga gatggagcgc
     2341 caaaacctcc tcaaaattgt ttgcgcctat cctgaatccg caggtcttga agaccatttg
     2401 cagattatta agtcggagat caatgacttt aagccagctc gtattgcaat cgactccctc
     2461 tctgctttgg cgcggggcgt tagcaacaat gccttccgcc aatttgtaat tggtgtcact
     2521 ggctacgcga aacaagaaga aatcacggga ctattcacaa ataccagtga tcaatttatg
     2581 ggagcgcatt cgattactga ctcccatatc tcaacaatta cggatacgat tatcttgctc
     2641 caatacgtcg agattcgtgg cgaaatgtcc cgcgccatta acgtcttcaa gatgcgcgga
     2701 tcttggcatg acaaagcaat ccgcgagttc atgatcagcg acaaagggcc ggacatcaag
     2761 gattctttcc ggaactttga gcggattatt tcaggttcgc caacacggat taccgtcgat
     2821 gagaaaagcg aactctcgcg aattgtgcgc ggcgttcaag aaaaagggcc ggagagctag
     2881 tag
//