LOCUS       BBa_J36336              2335 bp    DNA     linear       29-Nov-2024
DEFINITION  Lac+RBS+KaiB+Lac+RBS+KaiC
ACCESSION   BBa_J36336
VERSION     BBa_J36336.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     CDS             227..538
                     /label=BBa_J36802
     CDS             547..634
                     /label=end of LacI coding region (inactive)
     misc_feature    547..746
                     /label=BBa_R0010
     misc_feature    635..672
                     /label=CAP binding site
     promoter        683..688
                     /label=-35
     promoter        707..712
                     /label=-10
     misc_feature    712..746
                     /label=LacI binding site
     misc_feature    719..719
                     /label=start
     promoter        547..746
                     /label=BBa_R0010
     misc_feature    759..762
                     /label=conserved
     RBS             755..766
                     /label=BBa_B0034
     CDS             773..2335
                     /label=BBa_J36804
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatga gccctcgtaa
      241 aacctacatt ctcaagctct acgtcgccgg caatactcca aactcagtcc gtgccctcaa
      301 aacgctcaag aacattctcg aagttgaatt tcaaggtgtt tatgctctaa aggtgatcga
      361 tgttctcaaa aatcctcagt tggcagaaga ggataaaatc ctagcgacgc caaccctcgc
      421 caaggttcta ccactgcctg tccgacggat tattggtgat ttatccgacc gtgagaaagt
      481 tttgattggc cttgatttac tctacggcga acttcaagat tccgacgact tctaataata
      541 ctagagcaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
      601 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      661 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      721 ttgtgagcgg ataacaattt cacacatact agagaaagag gagaaatact agatgacttc
      781 cgctgagatg actagcccta ataataattc tgagcaccaa gccatcgcta agatgcgcac
      841 gatgattgaa ggctttgatg atattagtca tggcggtctt ccaatcgggc gatcgaccct
      901 cgttagtggt acttcaggaa ccggcaagac ccttttttct attcaatttc tctataacgg
      961 tattatcgag tttgatgagc ctggggtttt cgttactttc gaagaaaccc cgcaagatat
     1021 cattaaaaac gcccgtagtt ttggctggga tttagccaag ctggtcgatg agggcaaact
     1081 atttattctt gatgcttcac ccgatccaga aggtcaagag gttgttggcg gcttcgatct
     1141 ctctgctctg attgagcgga ttaattatgc aattcaaaag tatcgagcgc ggcgggtttc
     1201 aattgactcg gtcacgtccg ttttccagca atatgatgcc tcttctgtgg ttcgccgcga
     1261 actctttcgg ttggtagctc gcctaaaaca aattggggca actacggtca tgaccaccga
     1321 gcgtatcgag gaatatggcc cgatcgctcg ttacggtgtt gaggaatttg tctccgataa
     1381 cgtcgtgatt ctccgcaacg ttttggaagg ggagcgccgt cgccgcaccc tcgaaatcct
     1441 caagctacgt ggcaccagcc acatgaaagg ggaatatccg ttcacgatta cggatcatgg
     1501 catcaatatc ttcccgctcg gggcaatgcg ccttacgcag cgatcgtcga acgtgcgtgt
     1561 ttcatctggt gtcgtccgac tcgatgaaat gtgtggtggg ggcttcttta aggactcaat
     1621 cattctggca actggcgcta caggcactgg taaaactctg ttagttagcc gtttcgttga
     1681 gaatgcttgt gctaacaaag agcgggcgat tctgttcgct tatgaagagt cacgagctca
     1741 gctgctccgc aatgcctatt catggggaat ggactttgag gagatggagc gccaaaacct
     1801 cctcaaaatt gtttgcgcct atcctgaatc cgcaggtctt gaagaccatt tgcagattat
     1861 taagtcggag atcaatgact ttaagccagc tcgtattgca atcgactccc tctctgcttt
     1921 ggcgcggggc gttagcaaca atgccttccg ccaatttgta attggtgtca ctggctacgc
     1981 gaaacaagaa gaaatcacgg gactattcac aaataccagt gatcaattta tgggagcgca
     2041 ttcgattact gactcccata tctcaacaat tacggatacg attatcttgc tccaatacgt
     2101 cgagattcgt ggcgaaatgt cccgcgccat taacgtcttc aagatgcgcg gatcttggca
     2161 tgacaaagca atccgcgagt tcatgatcag cgacaaaggg ccggacatca aggattcttt
     2221 ccggaacttt gagcggatta tttcaggttc gccaacacgg attaccgtcg atgagaaaag
     2281 cgaactctcg cgaattgtgc gcggcgttca agaaaaaggg ccggagagct agtag
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